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ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.

Analisis Pengujian Biological Exygen Demand (BOD) dan Chemical Oxygen Demand (COD) di Inlet dan Outlet Instalasi Pengolahan Air Limbah (IPAL) RSU GMIM Tonsea Airmadidi Turambi, James S.; Naharia, Orbanus; Gedoan, Sukmarayu P.; Mokosuli, Yermia S.
Jurnal Pendidikan Tambusai Vol. 8 No. 2 (2024)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Rumah Sakit merupakan institusi pelayanan kesehatan bagi masyarakat. Limbah cair yang dihasilkan sebuah rumah sakit umumnya banyak mengandung virus, bakteri, obat-obatan dan senyawa kimia yang dapat membahayakan bagi kesehatan masyarakat sekitar. Hasil dari kualitas pengolahan limbah tidak terlepas dari dukungan dan sistem pengelolaan air limbah itu sendiri. Parameter pencemar air buangan rumah sakit yang perlu diperhatikan yaitu Biochemical Oxygen Demand (BOD), atau Kebutuhan Oksigen Biologi dan Chemical Oxygen Demand (COD). Rumah Sakit Umum GMIM Tonsea Airmadidi merupakan salah satu rumah sakit swasta milik GMIM yang ada di Kabupaten Minahasa Utara Provinsi Sulawesi Utara dan memiliki Instalasi Pengolahan Air Limbah untuk mengolah limbah cair. Penelitian ini termasuk penelitian cross sectional, bersifat observasional dengan tujuan untuk dapat mengetahui efektivitas sistem pengolahan limbah cair di Rumah Sakit Umum GMIM Tonsea. Hasil penelitian menunjukkan bahwa terdapat penurunan nilai BOD dan COD dari titik inlet ke titik outlet air limbah RSU GMIM Tonsea.

Identifikasi Teripang Laut (Holothuroidea) dari Perairan Laut Pulau Kabaruan Berdasarkan Identifikasi Morfologi dan DNA Barcoding Rattu, Ferencia Esananda; Mege, Revolson; Manampiring, Nonny; Roring, Verawati Ida Yani; Gedoan, Sukmarayu; Mokosuli, Yermia
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9649

Indonesia is known with its high biodiversity. One of them is sea cucumber. Various species of sea cucumbers are found in Indonesian sea in Talaud Regency at Kabaruan Island. Identification of sea cucumbers to reveal the biodiversity of sea cucumbers in Kabaruan Island, which is almost unknown. This research was to identify six samples of sea cucumber (Holothuroidea) in Kabaruan Island based on morphology and DNA barcoding using the COI gene. The differences between each species can be seen from the morphological characteristics, the length and width of sea cucumbers, papillae, pattern and color. The results showed that there were 4 species from 6 samples, which is Holothuria leucospilota, Holothuria whitmaei, Eleutherozoa sp., and Holothuria atra. That six samples with the code A1, A2, B1, C1, and C2 have a homology level of 99%-100% with the target nucleotide sequence from GenBank. Meanwhile, the sampleâ€™s code A3 has a homology level of 90% with the Eleutheroza sp. so there is a possibility that sample A3 is a new taxa that has the highest similarity to the Eleutheroza sp. From the six samples, it was found that samples A2 and B1 had the highest level of similarity which is 100%. Meanwhile, the samples with the lowest percentage values are samples A3 and B1 and samples A2 and A3, with a percentage value of 82.75%. There were 4 species from six samples found in Kabaruan Island. There is a possibility that Sample A3 is a new species that is similar to Eleutheroza sp.

Analysis of Dominant Number Values Weed Population in Paddy Rice Cultivation (Oryza sativa, L.) in Koya Village, Minahasa, North Sulawesi Kasim, Yuriko; Lihiang, Anatje; Gedoan, Sukmarayu; Tanor, Meity; Yalindua, Aser; Sumampouw, Herry
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9076

Paddy rice is the main food crop in Indonesia because rice is the primary food source of the Indonesian people. However, it faces obstacles caused, among others, by the presence of nuisance plants, namely weeds, that can reduce the quantity and quality of harvest or production. This study examines the value of the dominant Number of weed populations in rice field cultivation. The research method used was weed observation with analysis of the chief number value, using the square of a sample plot measuring 50 cm x 50 cm, randomly placed on the experimental land. The dominant number value analysis was carried out four times at the beginning of the study as an initial vegetation analysis, 21 DAP observations, 45 DAP observations, and 60 DAP observations. The results showed that the weed species with the highest Dominant Number Value at initial words in paddy fields were Paspalum disticum, Fibristylis litoralis, Marsilea crenata, Cyperus iria, and Ludwigia octovalvis; weed species with the highest Dominant Number Value at 21 DAP observations were P. disticum, Echinochloa crusgalli, F. litoralis, M. crenata, and Leersia hexandra; The weed species with the highest Dominant Number Value at 45 DAP observations were P. disticum, F. litoralis, C. iria, E. crusgalli, and Leersia hexandra and the weed species with the highest Dominant Number Values at 60 DAP observations were E. crusgalli, P. disticum, F. litoralis, Scirpus juncoides, and S. juncoides

CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.

ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.

Analysis of Dominant Number Values Weed Population in Paddy Rice Cultivation (Oryza sativa, L.) in Koya Village, Minahasa, North Sulawesi Kasim, Yuriko; Lihiang, Anatje; Gedoan, Sukmarayu; Tanor, Meity; Yalindua, Aser; Sumampouw, Herry
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9076

Paddy rice is the main food crop in Indonesia because rice is the primary food source of the Indonesian people. However, it faces obstacles caused, among others, by the presence of nuisance plants, namely weeds, that can reduce the quantity and quality of harvest or production. This study examines the value of the dominant Number of weed populations in rice field cultivation. The research method used was weed observation with analysis of the chief number value, using the square of a sample plot measuring 50 cm x 50 cm, randomly placed on the experimental land. The dominant number value analysis was carried out four times at the beginning of the study as an initial vegetation analysis, 21 DAP observations, 45 DAP observations, and 60 DAP observations. The results showed that the weed species with the highest Dominant Number Value at initial words in paddy fields were Paspalum disticum, Fibristylis litoralis, Marsilea crenata, Cyperus iria, and Ludwigia octovalvis; weed species with the highest Dominant Number Value at 21 DAP observations were P. disticum, Echinochloa crusgalli, F. litoralis, M. crenata, and Leersia hexandra; The weed species with the highest Dominant Number Value at 45 DAP observations were P. disticum, F. litoralis, C. iria, E. crusgalli, and Leersia hexandra and the weed species with the highest Dominant Number Values at 60 DAP observations were E. crusgalli, P. disticum, F. litoralis, Scirpus juncoides, and S. juncoides

CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.

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