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ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.

Identifikasi Teripang Laut (Holothuroidea) dari Perairan Laut Pulau Kabaruan Berdasarkan Identifikasi Morfologi dan DNA Barcoding Rattu, Ferencia Esananda; Mege, Revolson; Manampiring, Nonny; Roring, Verawati Ida Yani; Gedoan, Sukmarayu; Mokosuli, Yermia
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9649

Indonesia is known with its high biodiversity. One of them is sea cucumber. Various species of sea cucumbers are found in Indonesian sea in Talaud Regency at Kabaruan Island. Identification of sea cucumbers to reveal the biodiversity of sea cucumbers in Kabaruan Island, which is almost unknown. This research was to identify six samples of sea cucumber (Holothuroidea) in Kabaruan Island based on morphology and DNA barcoding using the COI gene. The differences between each species can be seen from the morphological characteristics, the length and width of sea cucumbers, papillae, pattern and color. The results showed that there were 4 species from 6 samples, which is Holothuria leucospilota, Holothuria whitmaei, Eleutherozoa sp., and Holothuria atra. That six samples with the code A1, A2, B1, C1, and C2 have a homology level of 99%-100% with the target nucleotide sequence from GenBank. Meanwhile, the sampleâ€™s code A3 has a homology level of 90% with the Eleutheroza sp. so there is a possibility that sample A3 is a new taxa that has the highest similarity to the Eleutheroza sp. From the six samples, it was found that samples A2 and B1 had the highest level of similarity which is 100%. Meanwhile, the samples with the lowest percentage values are samples A3 and B1 and samples A2 and A3, with a percentage value of 82.75%. There were 4 species from six samples found in Kabaruan Island. There is a possibility that Sample A3 is a new species that is similar to Eleutheroza sp.

The Antioxidant Activity of Red Fruit Extract (Pandanus conoideus L.) From Nabire Papua Migau, Natalina; Mokosuli, Yermia; Wurarah, Masje; Manampiring, Nonny
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9174

Red fruit (Pandanus conoideus Lam) is a native Papuan plant which has been used by indigenous Papuan people as a medicinal plant. Papuan people believe that red fruit has a broad spectrum of pharmacological activity. This research aims to obtain antioxidant activity, namely reducing DPPH free radicals from Papuan red fruit ethanol extract. Samples of red fruit obtained from Nabire Papua. After determination, the red fruit is extracted using the maceration method. The ethanol extract of red fruit was then used for the DPPH antioxidant test method. The results showed that the ethanol extract of red fruit contains phenolic compounds: flavonoids, tannins; and steroids in high intensity. Ethanol extract of red fruit has very strong antioxidant activity (46.99 ppm; R2=0.99). Ethanol extract of Papuan red fruit has potential as a source of bioactive natural antioxidants.

ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category

ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.

The Antioxidant Activity of Red Fruit Extract (Pandanus conoideus L.) From Nabire Papua Migau, Natalina; Mokosuli, Yermia; Wurarah, Masje; Manampiring, Nonny
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9174

Red fruit (Pandanus conoideus Lam) is a native Papuan plant which has been used by indigenous Papuan people as a medicinal plant. Papuan people believe that red fruit has a broad spectrum of pharmacological activity. This research aims to obtain antioxidant activity, namely reducing DPPH free radicals from Papuan red fruit ethanol extract. Samples of red fruit obtained from Nabire Papua. After determination, the red fruit is extracted using the maceration method. The ethanol extract of red fruit was then used for the DPPH antioxidant test method. The results showed that the ethanol extract of red fruit contains phenolic compounds: flavonoids, tannins; and steroids in high intensity. Ethanol extract of red fruit has very strong antioxidant activity (46.99 ppm; R2=0.99). Ethanol extract of Papuan red fruit has potential as a source of bioactive natural antioxidants.

ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category

Efficacy and Physiological Responses of Payangka Fish (Ophieleotris aporos) to Anesthetization with Clove Oil Rawung, Livana Dethris; Tanod, Wendy Alexander; Manampiring, Nonny
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.4726

This study aims to determine the effect of clove oil concentration and time of immersion in clove oil on the anesthesia, recovery time, and physiological responses in the payangka fish (Ophieleotris aporos). The experimental design was completely randomized with a 6 x 3 factorial arrangement. The first factor was a concentration of clove oil consisting of six levels i.e., 0.02; 0.03; 0.04; 0.05; 0.1, and 0.2 ml/L of water. The second factor was a time of immersion consisting of three levels i.e., 1; 3; and 6.5 hours. Results showed that the concentration of clove oil significantly (p<0.05) affected the anesthesia time and the recovery time after immersing. The length of immersing and concentration of clove oil also significantly (p<0.05) affected the concentration of glucose, but not significantly (p>0.05) affected the concentration of cholesterol. It showed that clove oil can be used as anesthetization for payangka fish in the appropriate dose. The optimal concentration of clove oil that can be used as anesthesia for the transportation of live payangka fish is 0.02 ml/L of water. Meanwhile, for handling activities on payangka fish, a clove oil concentration of 0.03 ml/L of water is recommended

Coconut Oil Processing as a Learning Medium and Implementation of P5 at SMA 1 Wori: Pengolahan Minyak Kelapa Sebagai Media Pembelajaran dan Implementasi P5 di SMA 1 Wori Mokosuli, Yermia; Mege, Revolson; Manampiring, Nonny; Matey, Meyer; Buyang, Dalle; Maidangkay, Vonny
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 9 No. 2 (2025): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar

North Sulawesi is identical with coconut plants, in addition to being a source of economy but also one of the leading commodities and inseparable from people's lives. However, until now coconut farmers still have difficulty getting quality and cheap consumption oil. Observing the problems of the community, State Senior High School 1 Wori raised it as material for the Pancasila Student Profile Strengthening Project (P5) Entrepreneurship Theme implementing coconut oil processing technology as well as a learning medium. However, the coconut oil processing carried out by students is still simple and of low quality. This activity aims to apply coconut oil processing technology as a learning medium and implementation of the Merdeka Belajar program at SMA 1 Wori to produce quality coconut oil. The method used is a combination of the Problem Based Learning method with the Life Skill Education approach to coconut processing entrepreneurship. Through this activity, students in addition to gaining knowledge and skills as well as direct experience of Life Skill Education to process coconut oil with technology and can produce quality coconut oil.

Anticancer Activity Of Apis Dorsata Binghami Nest Extract From West Uluway, Tana Toraja District Patandean, Selvrining; Mokosuli, Yermia Semuel; Ogi, Nova LIM; Djarang, Rievo H; Manampiring, Nonny; Moko, Emma Mauren
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12750

Cancer is the leading cause of death in the world, with a high prevalence in Indonesia, especially breast cancer. Conventional therapies such as chemotherapy and radiotherapy have various limitations, including severe side effects and cancer cell resistance. This study aims to explore the anticancer potential of the ethanol extract of Apis dorsata Binghami nests originating from Uluway Barat Village, Tana Toraja Regency. Extraction was carried out using the maceration method with 96% ethanol solvent, with a yield of 51.42%, and bioactive compounds were identified through GC-MS analysis. Anticancer activity tests were carried out in vitro on MCF-7 breast cancer cells using the resazurin assay method . The results showed that the extract contained various bioactive compounds such as flavonoids, furanones, and aromatic aldehydes which are known to have antioxidant and anticancer activities. The IC50 value obtained was 434.30 µg/mL, indicating weak cytotoxic activity. Nevertheless, this extract showed significant ability to kill cancer cells at certain concentrations, especially 15.63 µg/mL which reduced cell viability by 44.39%. These results indicate that A. dorsata Binghami nests contain compounds that have the potential as natural anticancer agents, but require further research for fractionation and identification of more specific active compounds and further testing in vivo

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