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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Biotechnology Planta Tropika Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology BIOTROPIC The Journal of Tropical Biology PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Sains dan Matematika Community Empowerment Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Jurnal Pengabdian, Riset, Kreativitas, Inovasi, dan Teknologi Tepat Guna (Parikesit)

Ganies Riza Aristya

Fakultas Biologi, Universitas Gadjah Mada, Yogyakarta

Author-ID : 1555570

Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Veterinary

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Karakterisasi Morfologi dan Anatomi Stroberi (Fragaria x ananassa D. cv. Festival) Hasil Induksi Kolkisin Ana Susianti; Ganies Riza Aristya; Sutikno S; Rina Sri Kasiamdari
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 2 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i2.929

Strawberry is a plant cultivated in subtropical countries, while Indonesia’s tropical climate. This is not accordance with the conditional of the natural growth of strawberries. Strawberries cultivar Festival is one of holticulture products in society. The high rates of consumption of strawberries are not equal with production and quality of strawberries. The quality of strawberries were determined by fruit size and uniformity of fruit. Effort that can be used to improve the qualityof strawberry production is polyploiditation with mutagenic substance, example colchicine. Result of polyploidy in morphological character are bigger. Technique of polyploidy with colchicine will be success if the number and structure of chromosom in cell nucleus are changes. Chromosome number changes will be change to anatomy, morphology and physiology plants. The aims of this research were to know the differences of anatomical and morphological characters of strawberry cultivar Festival after colchicine induction and find out the influence of the colchicine concentration differences in morphological and anatomical character of strawberry cultivars Festival. The research was have two main stages. The first were observation and scoring of 25 morphological characters of strawberries after colchicine induction with 0%; 0,01% and 0,05%. The second, preparations with paraffin method on the root, steam, leaves and fruit of the strawberry plant after colchicine induction. The observation of anatomical characters are measurements number cell was from of the root, steam, leaves and fruits. The results showed that induction of colchicine in strawberry cultivars festival showed different morphological characters on the density of the leaves, number of stems, fruit ripening time, fruit size, fruit sweetness of the outer color of the fruit, fruit acidity compared to control plants. Anatomical characters of cell size increases the cortex and pith of stems, leaves, cell mesofil cells of the cortex and pith of fruit. Colchicine induction of 0.05% was more effective in increasing the size of the fruit while the induction of colchicine of 0.01% was more effective at increasing the size of vegetative organs of strawberry cultivars Festival plants.Keywords: anatomy, colchicine, Festival Cultivars, morphology, strawberry

Karakterisasi Kromosom Stroberi (Fragaria vesca L. subsp. californica Cham. & Schltdl. cv. Californica) Hasil Poliploidisasi Rosyidatul Khoiroh; Ganies Riza Aristya; Sutikno S; Niken Satuti Nur Handayani
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 2 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i2.932

Fruit consumption such as strawberries always increase in Indonesia and reaching about 210 tons in 2011. Strawberry (Fragaria vesca L. subsp. californica Cham. and Schltdl. cv. Californica) has been developed in Indonesia, especially in Banyuroto, Sawangan, the district of Magelang, Central Java. In order to improve the fruit productivity (quality and quantity), geneticcharacterization including chromosomes character of this fruit are needed to be investigate. Therefore, the research was conducted to determine the chromosomes number, chromosomes size chromosomes shape and karyotype of control and strawberries treated by colchicine as mutagenic agent. The method was squash and flow cytometry method. Whereas the cell suspension samplewere observed used flow cytometer instrument. The result of research shown that the chromosome number of strawberry Californica in normal and treatment strawberries was 2n=14. Thechromosomes shape of normal strawberry was 10 metacentric and 4 submetacentric. Whereas the chromosomes shape of treatment strawberries used colchicine was 10 metacentric and 4submetacentric. Flow cytometric analysis shown that control strawberries and treated strawberries has diploid ploidy level.Keywords: chromosomes, colchicine, flow cytometry, polyploidization, strawberry Californica

Analisis Kandungan Vitamin Pada Melon (Cucumis melo L.) Kultivar Melodi Gama 1 dan Melon Komersial Budi S Daryono; Sigit Dwi Maryanto; Sholihatun Nissa; Ganies Riza Aristya
Biogenesis: Jurnal Ilmiah Biologi Vol 4 No 1 (2016)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v4i1.1113

As many as 50 million people worldwide are currently experiencing a deficiency of vitamin A and about 250000-500000 malnourished children in developing countries become blind each year from vitamin A deficiency, with the highest prevalence in Southeast Asia and Africa which will result in visual impairment, and increased child mortality and pregnant women. Melon can be one of the solutions for higher productivity and fruit may contain carotene (pro-vitamin A) with varying levels. In the years 2009-2011 Genetics laboratory, faculty of Biology UGM has produced a new melon cultivar called Melodi Gama 1 (MG 1). MG 1 phenotype has a rounded character with rather thick rind and hard, green and fleshy with a smooth orange flesh color. The purpose of this research was to study and analyze the nutritional content of MG 1 compared to commercial cultivars from multilocation and multiseason cultivation. The methods were used vitamin A quantitative analysis by extraction and spectrophotometer, whereas vitamin C in quantitative analysis using the iodine titration method. The results obtained in the analysis of total carotene in the dry season for the MG 1 has the highest value compared with 4 other commercial cultivars, it was 706.61 μg/100gr for cultivation in Yogyakarta and 292.16 μg/100gr for cultivation in Magetan. In the rainy season total carotene MG 1 was 59.88 μg/100gr for cultivation in Yogyakarta, and 20.76 μg/100gr for cultivation in Magetan. The results of the quantitative analysis of vitamin C MG 1 in the dry season was 7.50 mg/100gr for cultivation in Yogyakarta and 16.50 mg/100gr for cultivation in Magetan, while the rainy season was 8.55 mg/100gr for cultivation in Yogyakarta and 6.75 mg/100gr for cultivation in Magetan. Based on these results it can be concluded that the content of Vitamin A and C from Melodi Gama 1 cultivar is higher in the dry season than in the rainy season.

Chromosome Characterization of Festival Strawberry (Fragaria x ananassa D. var. Festival) Result of Polyploidization Ganies Riza Aristya; Rezika Alyza
Biogenesis: Jurnal Ilmiah Biologi Vol 7 No 1 (2019)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i1.5250

Strawberry is fruit commodities which originating from the member of Rosaceae and has been widely cultivated in several countries of the world including Indonesia. One of the strawberry cultivars which consumed and cultivate in Indonesia is the strawberry Festival (Fragaria x ananassa D. var. Festival), which was developed in the area of Banyuroto, Magelang. In order to improve the quality and quantity of strawberry plants, polyploidization using colchicine has been employed in previous studies. The objectives of this research were study the chromosome character which include number, size, shape, and karyotype of normal strawberries and treatment strawberries with colchicine (0.05% 36 hours of root and leaf induction, 0.05% 24 hours of root and leaf induction, 0.05% 36 hours of leaf induction, 0.01% 36 hours of root and leaf induction). The results of this research shown that chromosome number difference and degree of ploidy between Fragaria x ananassa var Chandler in America with Fragaria x ananassa var. Festival in Indonesia due to natural mutation. While Festival strawberry control and treatment colchicine have same number chromosome is 2n=4x=28M and there are a difference long sleeve and short sleeve chromosome that may affect the content of chromosome expression and phenotype character are produced. The analysis of flow cytometry showed that treatment strawberry with colchicine has two peaks with intensity fluorescence equal to strawberry control so that it can be interpreted that the ploidy number of strawberries treatment with colchicine is tetraploid.

Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Budi Setiadi Daryono; Ganies Riza Aristya; Rina Sri Kasiamdari
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5

Implementasi Inovasi Budidaya Stroberi di Agrowisata Banyuroto Kabupaten Magelang Melalui Education for Sustainable Development Ganies Riza Aristya; Aries Bagus Sasongko; Lisna Hidayati; Agus Setiawan
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

The cultivation of strawberry needs of technological innovation to gain competitive advantage strawberry fruit quality and quantity. This is motivated because the decline in the production of strawberries in the area and quality of strawberry fruit is still low. Since 2012 until now, Laboratory of Genetics has identified and developed the character of phenotype and genotype of strawberry plants in Agro Banyuroto, Village Banyuroto Sawangan District of Magelang regency. Character genotypes studied through the identification of ploidy with cytogenetic approach and apply poliploidisasi technology that aims to double the number of sets of chromosomes in order to obtain a plant that has the character of superior phenotype compared with the control. The purpose of this activity was to awaken the capacity of communities that were able to develop and utilize natural resources to meet human needs while maintaining the environmental implementing action plans that lead to sustainable development in a sustainable manner. The method was executed in this activity was the cultivation of strawberries by exploiting natural resources Banyuroto village environment that was using bamboo, strawberry cultivation techniques with vertikultur systems. Moreover, the carrying capacity was also supported by the promotion of village tourism as central region Banyuroto strawberry cultivation of strawberries superior to tourists every week, promotion through Online system (in the network) by utilizing Information Technology and Computing and community empowerment with the diversification of food made from strawberries. The end result of this activity was obtained hallmarks of crops polyploidy were plant growth faster, the ability of the formation of stolons and nursery faster, leaf area and stem diameter was wider, the stem stout and roots were longer, the fruit produced was greater and a sweeter taste with more red fruit color. Innovation strawberry crop is expected to be followed by the cultivation of sustainable optimal as possible so that the results obtained are able to improve the welfare of farmers in Banyuroto strawberries.

Identifikasi Sifat dan Distribusi Sel Endokrin Ghrelin di Lambung Tikus (Rattus norvegicus): Studi Immunohis-Tokimia pada Kondisi Obesitas Teguh Budipitojo; Hevi Wihadmadyatami; Ganies Riza Aristya
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Obesity is one of major nutritional problems in the world. Obesity is very dangerous, especially when concentrated in the abdomen, because it is closely linked to various diseases such as diabetes, hypertension, heart disease, which can to causing death. This study aims to identify the nature and distribution of ghrelin on gastric endocrine cells in the obese rat (Rattus norvegicus) by using immunohistochemical techniques. The results will strengthen the understanding of the role and function of ghrelin as an alternative therapeutic target on obesity. The research used gastric tissues of ten obese and control rats which were stained with avidin-biotin-peroxidase complex method of immunohistochemistry. The results showed the existence of two types of ghrelin-producing cells (open and closed types) on the gastric mucosa of control rats, and only one type of ghrelin producing cells (open type) in obese rats. The intensity of ghrelin immunoreactive positive cells was detected weak in obese rats, but very strong in control rats. Ghrelin endocrine cells mainly distributed in the basal part of the gastric mucosa of the fundus parts, with a very small number in obese rats, but highly abundant in control rats. This study confirmed the decrease of the ghrelin synthesis and secretion in obese rat (Rattus norvegicus) at the cellular level. The decrease of ghrelin synthesis is characterized by a reduction on the number of ghrelin producing cells, the disappearance of the close type of ghrelin producing cells, and the low activity of protein synthesis in the ghrelin producing cells. Ghrelin endocrine cells distributed mainly in the basal part of the gastric mucosa, especially in the fundus parts.

Karakter Gen CmBG1 Melon (Cucumis melo) pada Pengaruh Cekaman Tanah Karst Ganies Riza Aristya; Budi Setiadi Daryono; Yuanita Rachmawati
Sains dan Matematika Vol. 3 No. 1 (2014): Oktober, Sains & Matematika
Publisher : Universitas Negeri Surabaya

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Lahan kritis berkapur, karst, memberikan cekaman abiotik pada tanaman karena hambatan hidrasi dan nutrisi. Asam absisat (ABA) adalah hormon yang diekspresikan tumbuhan pada kondisi cekaman abiotik. CmBG1 merupakan salah satu gen peregulasi hormon ABA pada melon yang akan terakumulasi saat tumbuhan mengalami cekaman. Tujuan penelitian ini adalah mendeskripsikan pengaruh lahan kritis karst terhadap ekspresi gen CmBG1 secara kualitatif dan kuantitatif melon hasil turunan kultivar TACAPA, yaitu kultivar PT dan AT yang ditanam pada medium tanah karst dari wilayah Agroekosistem II dan III Yogyakarta (Gunungsewu, Dlingo, maupun Sentolo). cDNA library diperoleh dari reverse transcription isolat RNA. cDNA diamplifi kasi dengan primer spesifi k, kemudian dispektrofotometri pada Î»260 nm untuk mengetahui konsentrasi gen CmBG1. Ekspresi gen dianalisis dengan real time PCR dengan gen referensi Cm-actin. Uji kualitatif dilakukan dengan elektroforesis gel agarosa 1,5%. Hasil penelitian menunjukkan gen CmBG1 terdeteksi dengan ukuran ±1258 bp pada kultivar PT dan AT. Konsentrasi gen CmBG1 melalui spektrofotometri menunjukkan semua kultivar yang ditanam pada media kontrol memiliki konsentrasi yang lebih rendah bila dibandingkan media tanam dengan perlakuan lahan kritis baik Gunungsewu, Dlingo, maupun Sentolo. Hasil ini sama dengan uji ekspresi gen CmBG1 menggunakan analisis kuantitatif real time PCR. Karsts critical land gives abiotic stresses in plants because of hydration and nutrition disturbances. Abscisic acid (ABA) is a hormone that expressed in the plant in abiotic stress conditions. CmBG1 is one of the regulatory genes encoding hormone ABA in melon plants which is accumulated in stress condition. The purpose of this study was to describe the infl uence of karsts critical land on the genes expression of CmBG1 melon cultivars PT and AT qualitatively and quantitatively. The plants were grown in medium karst land of Agroecosystems II and III of Yogyakarta (Gunungsewu, Dlingo, and Sentolo). Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplifi ed using specifi c primer. Spectrophotometry was conducted in Î»260 nm and electrophoresis run in 1.5% agarose gel. Control band and reference gene chosen in Real Time PCR was Cm-Actin. CmBGI band (± 1258 bp) was showed both on PT and AT. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar PT and AT melons when they are grown under stress condition. This result show similarly when using real time PCR.

Chromosome Characterization of Brassicaceae Family Ganies Riza Aristya; Bening Larasati; Galang Riswi Dyatama; Himawan Masyhuri; Febri Yuda Kurniawan; Fauzana Putri; Dian Astriana; Yuanita Rachmawati
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Indonesia is known as a rich country in various agricultural and plantation products, including vegetables such as mustard, broccoli, cabbage, and cauliflower. However, in its cultivation, the products and demand for vegetables are not offset by an increase in the production quality. One of the efforts to improve and enhance the production quality is to identify and characterize chromosomes of plants which will become the basis for plant breeding activities. The purpose of this study was to characterize the number, form and size of the chromosome in cultivars belonging to the Brassicaceae family. The study was carried out using the modified squash method. Chromosomes were prepared by fixation, maceration, and staining, then the mitotic phases were observed using a microscope and optilab, and analyzed using Image Raster 3. The results showed that mitotic time range and chromosome character of six cultivars of the Brassicaceae family were different. Broccoli ('Chief No. 2 1955' and 'Green Super') and cauliflower ('ILONA' and 'TM 126') had a mitotic time range from 04.00 to 09.00 a.m. with 2n chromosome number = 18. Green mustard (‘Juwita’ and ‘TM Jade’) and white mustard ('Sakata' and 'Shuka-shuka') had a mitotic time range from 03.00 to 08.00 a.m. with 2n chromosome number = 20. White cabbage (‘CR ACE' and 'Sehati F1') had a mitotic time range from 04.00 to 09.00 a.m and red cabbage (‘Scarlet’ and ‘Red Globe’) had a mitotic time range from 09.00 to 10.00 a.m. with 2n chromosome number = 18.

Development of ecotourism in Blubuk Village through sustainable environmental management Aristya, Ganies Riza; Wulandari, Mutiara Tri; Dimas, Abimanyu; Sritanjung, Opi; Ramadhani, Gilang Afif Setya; Ishana, Aurelia Araya; Fadhlurrohman, Galih Dzaky; Azzahira, Faiha Azka; Muliawati, Adina; Sofyantoro, Fajar
Community Empowerment Vol 10 No 2 (2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.12321

Blubuk Hamlet, Kulon Progo is a rural area with great potential in agriculture and ecotourism. However, the community faces challenges in maximizing this potential due to limited knowledge. This community service program aims to empower the local community through waste utilization to have economic value. The program uses various methods, including training, participatory mapping, and focus group discussions (FGDs). The main activities include maggot cultivation for fish feed production, biodiesel production from used cooking oil, eco-enzyme production from organic waste, and ecotourism planning. The results showed that 80% of training participants successfully understood maggot cultivation techniques, collectively produced 3 kg of maggots, and produced 1 kg of fish pellets in two months. Eco-enzyme production from household organic waste reduced waste by 40%, with 85% of participants using the product as a natural cleaner and fertilizer. Biodiesel production produced 2 liters of biodiesel from 5 liters of used cooking oil, which was tested and used as an alternative fuel. This program successfully introduced the concept of community-based ecotourism management and increased public understanding of the economic potential that can be developed from local natural resources.

Co-Authors Abdul Razaq Chasani, Abdul Razaq Abiyyi, Muhammad Hawi Qabus Afifah, Nasywa Kamila Agus Setiawan Almira Syafataya Ana Susianti Ani Widiastuti, Ani Aries Bagus Sasongko Aurantika, Rindu Ayundai, Melin Ayundai, Melin Azzahira, Faiha Azka Bartolomius Renaldy Bening Larasati Budi S Daryono BUDI SETIADI DARYONO Chalvia Zuyyina Christophorus Ardhito Haryo Dwinanda Dea Febiansi Dewi, Umi Rosita Dian Astriana Dimas, Abimanyu Dita Yulistiani Elva Maulida Khozana Fadhlurrohman, Galih Dzaky Fajar Sofyantoro Fauzana Putri Febri Yuda Kurniawan Galang Riswi Dyatama Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hevi Wihadmadyatami Hidayat, Madyan Akmal Himawan Masyhuri Inayati, Evi Indra Lesmana Ishana, Aurelia Araya Karmilah, Karmilah Kusnah Dian Prasiwi Latifah, Vida Rahma Lisna Hidayati Mif Ardianata Pratama Putra Muhammad Mufid Al-Dayyan Muhammada, Khusnus Syifa Muliawati, Adina Nainggolan, Ananto Puradi Nasywa Kamila Afifah Niken Satuti Nur Handayani Niken Satuti Nur Handayani Nugroho, Rifat Rizki Adi Nugroho Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan Pratiwi, Arini Dian Putri, Fauzana Putri, Fauzana Ramadhani, Gilang Afif Setya Rezika Alyza Rifa Ayuningsih Rina Sri Kasiamdari Rispaini Nur Aziza Rosyidatul Khoiroh Safitri, Della Thasya Liona Sholihatun Nissa Sigit Dwi Maryanto Sofyantoro, Fajar Sritanjung, Opi Sutikno S Syafei, Dio Rachmaputra Teguh Budipitojo Triska Ayu Nurwijayanti Tuty Arisuryanti, Tuty Ulfa, Siti Zakia Uswatun Mujahidah Utami, Mia Widiastuti, Ani Widyasari, Adristi Shafa Wulandari, Mutiara Tri Yuanita Rachmawati Yusuf, Adib Fakhruddin

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