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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Biotechnology Planta Tropika Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology JMM (Jurnal Masyarakat Mandiri) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Sains dan Matematika Community Empowerment Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Jurnal Pengabdian, Riset, Kreativitas, Inovasi, dan Teknologi Tepat Guna (Parikesit) BIOTROPIC
Ganies Riza Aristya
Fakultas Biologi, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Veterinary Other

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Molecular insights into the biodiversity of freshwater red macroalgae in Indonesia Aristya, Ganies Riza; Nugroho, Rifat Rizki Adi Nugroho; Chasani, Abdul Razaq
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.38211

Abstract

Many Asian countries, including Indonesia, have experienced negative effects from pollution brought on by industrialization and modernization, which have harmed freshwater ecosystems as well as natural habitats. Red macroalgaeand other creatures that depend on these ecosystems for their survival may suffer as a result, which could increase the strain on freshwater ecosystems and resources. Due to freshwater red macroalgaeare primary producers in their own ecosystems, they are essential to the cycling of nutrients and the preservation of water quality. The objective of this study is to evaluate freshwater red macroalgae in Indonesia utilizing various approaches particularly emphasis on DNA barcoding. As a result, molecular methods like DNA barcoding are employed to uncover hidden species and elucidate the evolutionary connections between different taxa, thereby exposing the diversity of red macroalgae. It is anticipated that the outcomes will yield data for further research.Keywords:DNA barcodeFreshwaterHidden speciesMolecularRed macroalgae
INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS Yusuf, Adib Fakhruddin; Latifah, Vida Rahma; Nurcahyati, Vivi Indah; Nurhasanah, Anggun Diyan; Widyasari, Adristi Shafa; Nainggolan, Ananto Puradi; Hasibuan, Aldy Riau Wansyah; Hidayat, Madyan Akmal; Karmilah, Karmilah; Pratiwi, Arini Dian; Aurantika, Rindu; Hasanah, Muslifah; Aristya, Ganies Riza; Handayani, Niken Satuti Nur; Arisuryanti, Tuty; Lesmana, Indra; Daryono, Budi Setiadi
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2362

Abstract

Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061. ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.
PEMANFAATAN DAUN KELOR UNTUK PENINGKATAN KESEJAHTERAAN MASYARAKAT DI KULON PROGO Aristya, Ganies Riza; Salsabila, Sophia; Risydah, Fiqiha; Amadeus, Sovann; Akbar, Ananda Malik; Tasyarofa, Alya; Yuda, Khalid Abdillah Abdurahman; Dyanita, Anissa Cahyadevi; Sofyantoro, Fajar
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 3 (2024): Juni
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i3.22884

Abstract

Abstrak: Sebagian besar masyarakat Kulon Progo bekerja sebagai petani dan peternak. Padukuhan Gegunung di Kulon Progo memiliki tanah yang subur. Tanaman kelor kaya akan nutrisi, memiliki akar yang kuat, dan dapat dimanfaatkan sebagai alternatif pakan ternak. Program pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman masyarakat terkait budidaya kelor serta pemanfaatannya dalam kehidupan sehari-hari. Kelompok sasaran dan lembaga yang menjadi mitra adalah kelompok ibu-ibu PKK. Jumlah peserta yang menjadi sasaran dari program ini berjumlah sekitar 50 orang. Kegiatan pengabdian masyarakat terbagi dalam tiga tahap, yakni survei awal dan perizinan, sosialisasi dan pelatihan, serta monitoring dan evaluasi. Sosialisasi dan pelatihan dilakukan dengan pemberian materi, diskusi, dan praktek langsung serta ditutup dengan pembagian bibit tanaman kelor. Evaluasi dilakukan melalui observasi peserta, wawancara, dan pengisian angket. Melalui program pengabdian ini, masyarakat berhasil memahami manfaat penggunaan tanaman kelor dalam kehidupan sehari-hari dan sanggup untuk menanam kelor di lingkungan sekitar dengan bibit yang telah dibagikan. Abstract: The community of Kulon Progo primarily engages in farming and livestock breeding. Gegunung Village, with its fertile soil, is ideal for cultivating moringa plants, which are nutrient-rich and can serve as alternative livestock feed. Our community service team conducted an educational outreach on the cultivation and benefits of moringa to enhance local understanding and usage. The program targeted the PKK women's group, involving approximately 50 participants. It was divided into three phases: initial survey, socialization and training, and monitoring and evaluation. The socialization and training phase included educational materials, discussions, and hands-on practice, concluding with the distribution of moringa seeds. Evaluation was conducted through participant observation, interviews, and questionnaires. Through this community service program, participants gained a comprehensive understanding of the benefits of moringa plants in daily life and demonstrated the ability to cultivate moringa in their local environment using the distributed seeds.
CmBGI Gene Expression encoding β-glucosidase in melon (Cucumis melo L.) under stress condition Yuanita Rachmawati; Ganies Rizaa Aristya; Budi Setiadi Daryono
Biotropic : The Journal of Tropical Biology Vol. 1 No. 2 (2017): Biotropic, Volume 1, Nomor 2, 2017
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2017.1.2.1-8

Abstract

CmBGI is the enzymatic genes encoding β-glucosidase that involved in Abscisic Acid (ABA) metabolism of Cucumis melo L. β-glucosidase promotes the accumulation of glucose, fructose, and sucrose, and it might act as a regulator that mediates melon fruit ripening both climacteric and nonclimacteric. ABA mediates adaptive responses to abiotic and biotic stresses. Agricultural Balitbang in 1997 showed that there were approximately 158.600 ha of degraded land scattered in three zones of agroecosystems in Yogyakarta (DIY). One of them is Dlingo Bantul area which has a karst type critical land area. Karst provides stress to the certain plant growth. One way to conserve critical land is making this area for agriculture. Cultivar TACAPA and TA were superior melons that have been developed by Genetic Laboratory of Biology Faculty UGM. This preliminary research was conducted to examine molecular characterization of CmBGI gene expression in cultivar TACAPA and TA which are planted in normal condition medium and in critical land medium treatment. Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplified using specific primer. Spectrophotometry was conducted in λ260 nm and electrophoresis run in 1.5% agarose gel. Control of band chosen was Cm-Actin. CmBGI gene concentration of TACAPA and TA in normal condition medium are in succession 578.5 and 579.4 μg/ml then for critical land medium treatment 743.4 and 773.5 μg/ml. CmBGI band was showed both of TACAPA and TA as ± 1258 bp. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar TACAPA and TA melons when they are grown under stress condition.
Karakterisasi Kromosom Spesies Anggota Familia Solanaceae Ganies Riza Aristya; Chalvia Zuyyina; Dea Febiansi; Rifa Ayuningsih; Kusnah Dian Prasiwi; Triska Ayu Nurwijayanti; Uswatun Mujahidah; Bartolomius Renaldy
Biotropic : The Journal of Tropical Biology Vol. 3 No. 1 (2019): Biotropic, Volume 3, Nomor 1, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2019.3.1.24-38

Abstract

Indonesia is known as a country rich in various agricultural and plantation products, including vegetables. One effort to improve quality of the production by identifying and characterizing chromosomes. The purpose study to characterize number, shape and size of the chromosomes in Solanaceae family. The study using modified Squash method. Chromosome preparation including fixation, maceration, staining, and analyzed using Image Raster 3 application, then Corel Draw X6. The results showed study to characterize the number, shape and size in the Capsicum annum L. Tm 999 (2n=20m+4sm and 2,41 s/d 9,99 µm) then Capsicum frutescens L. Kencana (2n=16m+8sm and 3,12 s/d 10,49 µm). In Solanum lycopersicum Or Diana and Marta (2n=16m+8sm and 3,12 s/d 10,49 µm) then (2n=22m+2sm and 2,65 s/d 7,28 μm). In Lycopersicon esculentum Mill. Juliet and Tropical Ruby (2n=18m+6sm and 0,77 s/d 1,81 μm) then (2n=20m+4sm and 0,39 s/d 0,94 μm). Solanum melongena L. Or Valerie and Mustang (2n=24m and 0.29 s/d 0.87 μm) then (2n=22m+2sm and 0,5 s/d 1.28 μm). In Solanum melongena L. Kania and Pulus (2n=20m+4sm and 0.64 s/d 3.43 μm) then (2n=16m+8sm and 0.84 s/d 2.51 μm). In Solanum melongena L. Jeno and Planet Hijau (2n=18m+6sm and 0.65 s/d 2.423 μm) then (2n=14m+10sm and 0.46 s/d 1.73 μm). In Capsicum annum var. Grossum L. Red Star and Purple Star (2n=22m+2sm and 3.19 s/d 8.49 μm) then (2n=24m and 5.5 s/d 11.92 μm).
Chromosome Characterization of Brassicaceae Family Ganies Riza Aristya; Bening Larasati; Galang Riswi Dyatama; Himawan Masyhuri; Febri Yuda Kurniawan; Fauzana Putri; Dian Astriana; Yuanita Rachmawati
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesia is known as a rich country in various agricultural and plantation products, including vegetables such as mustard, broccoli, cabbage, and cauliflower. However, in its cultivation, the products and demand for vegetables are not offset by an increase in the production quality. One of the efforts to improve and enhance the production quality is to identify and characterize chromosomes of plants which will become the basis for plant breeding activities. The purpose of this study was to characterize the number, form and size of the chromosome in cultivars belonging to the Brassicaceae family. The study was carried out using the modified squash method. Chromosomes were prepared by fixation, maceration, and staining, then the mitotic phases were observed using a microscope and optilab, and analyzed using Image Raster 3. The results showed that mitotic time range and chromosome character of six cultivars of the Brassicaceae family were different. Broccoli ('Chief No. 2 1955' and 'Green Super') and cauliflower ('ILONA' and 'TM 126') had a mitotic time range from 04.00 to 09.00 a.m. with 2n chromosome number = 18. Green mustard (‘Juwita’ and ‘TM Jade’) and white mustard ('Sakata' and 'Shuka-shuka') had a mitotic time range from 03.00 to 08.00 a.m. with 2n chromosome number = 20. White cabbage (‘CR ACE' and 'Sehati F1') had a mitotic time range from 04.00 to 09.00 a.m and red cabbage (‘Scarlet’ and ‘Red Globe’) had a mitotic time range from 09.00 to 10.00 a.m. with 2n chromosome number = 18.    
Molecular insights into the biodiversity of freshwater red macroalgae in Indonesia Aristya, Ganies Riza; Rifat, Nugroho Rizki Adi; Abdul, Chasani Razaq
Depik Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.38211

Abstract

Many Asian countries, including Indonesia, have experienced negative effects from pollution brought on by industrialization and modernization, which have harmed freshwater ecosystems as well as natural habitats. Red macroalgaeand other creatures that depend on these ecosystems for their survival may suffer as a result, which could increase the strain on freshwater ecosystems and resources. Due to freshwater red macroalgaeare primary producers in their own ecosystems, they are essential to the cycling of nutrients and the preservation of water quality. The objective of this study is to evaluate freshwater red macroalgae in Indonesia utilizing various approaches particularly emphasis on DNA barcoding. As a result, molecular methods like DNA barcoding are employed to uncover hidden species and elucidate the evolutionary connections between different taxa, thereby exposing the diversity of red macroalgae. It is anticipated that the outcomes will yield data for further research.Keywords:DNA barcodeFreshwaterHidden speciesMolecularRed macroalgae
Co-Authors Abdul Razaq Chasani, Abdul Razaq Abdul, Chasani Razaq Abiyyi, Muhammad Hawi Qabus Afifah, Nasywa Kamila Agus Setiawan Akbar, Ananda Malik Almira Syafataya Amadeus, Sovann Ana Susianti Ani Widiastuti, Ani Aries Bagus Sasongko Aurantika, Rindu Ayundai, Melin Ayundai, Melin Azzahira, Faiha Azka Bartolomius Renaldy Bening Larasati Budi S Daryono BUDI SETIADI DARYONO Chalvia Zuyyina Christophorus Ardhito Haryo Dwinanda Dea Febiansi Dewi, Umi Rosita Dian Astriana Dimas, Abimanyu Dita Yulistiani Dyanita, Anissa Cahyadevi Elva Maulida Khozana Fadhlurrohman, Galih Dzaky Fajar Sofyantoro Fauzana Putri Febri Yuda Kurniawan Galang Riswi Dyatama Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hevi Wihadmadyatami Hidayat, Madyan Akmal Himawan Masyhuri Inayati, Evi Indra Lesmana Ishana, Aurelia Araya Karmilah, Karmilah Kusnah Dian Prasiwi Latifah, Vida Rahma Lisna Hidayati Mif Ardianata Pratama Putra Muhammad Mufid Al-Dayyan Muhammada, Khusnus Syifa Muliawati, Adina Nainggolan, Ananto Puradi Nasywa Kamila Afifah Niken Satuti Nur Handayani Niken Satuti Nur Handayani Nugroho, Rifat Rizki Adi Nugroho Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan Pratiwi, Arini Dian Putri, Fauzana Putri, Fauzana Ramadhani, Gilang Afif Setya Rezika Alyza Rifa Ayuningsih Rifat, Nugroho Rizki Adi Rina Sri Kasiamdari Rispaini Nur Aziza Risydah, Fiqiha Rosyidatul Khoiroh Safitri, Della Thasya Liona Salsabila, Sophia Sholihatun Nissa Sigit Dwi Maryanto Sofyantoro, Fajar Sritanjung, Opi Sutikno S Syafei, Dio Rachmaputra Tasyarofa, Alya Teguh Budipitojo Triska Ayu Nurwijayanti Tuty Arisuryanti, Tuty Ulfa, Siti Zakia Uswatun Mujahidah Utami, Mia Widiastuti, Ani Widyasari, Adristi Shafa Wulandari, Mutiara Tri Yuanita Rachmawati Yuda, Khalid Abdillah Abdurahman Yusuf, Adib Fakhruddin