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Detection of rpoB Gene Mutations in Mycobacterium Tuberculosis widodo, widodo; Riadi, Ahmad; Etevia Purlinda, Devi; Setyowatiningsih, Lilik; roniafriansya, roni
Journal of Applied Health Management and Technology Vol. 5 No. 3 (2023): July 2023
Publisher : Postgraduate Program , Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jahmt.v5i3.10093

Abstract

Perkembangan pengobatan infeksi tuberculosis memberikan harapan kesembuhan total akan tetapi seiring berjalanya waktu pengunaan obat berdampak pada munculnya strain Mycobacterium tuberculosis yang resisten terhadap obat peningkatan kecepatan diaknostik terhadap resistensi perlu dikembangkan salah satunya methode molekuler berbasis PCR penentu mutasi gen yang disebakan resistensi obat salah satu target obat yang digunakan adalah rpoB, pengunaan Primer rpoB sebagai penanda terjadinya mutasi diharapkan mampu memberikan dampak sebagai alternatif deteksi cepat secara molekuler. Panjang pita rpoB 318 bp cocok untuk kelompok Mycobacterium tuberculosis diperoleh hasil menunjukan pada kodon 509 perubagan AGC menjadi AAT terjadi perubahan asam amino dari serin menjadi Apargarin, perubahan nukleotida  CAG menjadi CCG pada kodon 510 mengubah asam amino Glutamin menjadi prolin, kodon 514 perubahan nukleotida TTC menjadi TTG mengubah asam amino Phenylalanine menjadi leucine, kodon 515 terjadi perubahan nukleotida ATG menjadi GTG perubahan ini menyebakan perubahan asam amino Methionine menjadi Valine  . perubahan tersebut menyebabkan terjadinya resistensi terhadap obat Rifampicin
Factors Related to Soil Transmitted Helminth Infection in Vegetable Farmers Wikandari, Ririh Jatmi; Setyowatiningsih, Lilik; Djamil, Masrifan; Surati, Surati; Kahar, Fitriani
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.2145

Abstract

Soil Transmitted Helminth (STH) infection can occur at all ages, from children to the elderly. Farmers are at risk of contracting STH infection because of their daily work which is in direct contact with the soil. The risk of STH infection is due to poor personal hygiene and environmental sanitation. The study aims to determine observe the factors associated with STH infection in vegetable farmers, using a cross-sectional approach. The research subjects were vegetable farmers in Batur Wetan Hamlet, Getasan, Semarang Regency. Data were collected using a questionnaire to determine the personal hygiene and sanitation hygiene of farmers when working in the garden. Worm identification used (reference,) based on the worm performance using the floating method. Worm identification were done by examining the stool using the floating method. The results of the study were processed using SPSS version 20. Data analysis using Chi-Square (α = 5%), found 3 positive study subjects infected with STH, consisting of 1 egg of Ascaris lubricoides and 2 eggs of Trichuris trichiura. Positive STH respondents, have a washing by water and soap habits before eating and defecating, without wearing gloves. There was a relationship between hand washing by water and soap habits before eating to worm infection, but no relationship between this habit after defecating and no wearing gloves to worm infection. Vegetable farmers are advised to change the daily habits and maintain personal hygiene after working. use personal protective equipment when working and maintain personal hygiene.