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UJI AKTIVITAS ANTIBAKTERI Staphylococcus aureus BERDASARKAN PERBEDAAN JENIS PELARUT EKSTRAK BIJI BUAH PEPAYA (Carica papaya L.) Lisa Anjani, Ni Putu; Suyasa, I Nyoman Gede; Habibah, Nur; Purna, I Nyoman
Jurnal Skala Husada : The Journal of Health Vol 22, No 1 (2025): Jurnal Skala Husada (JSH): The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v22i1.3946

Abstract

Staphylococcus aureus is an opportunistic pathogenic bacteria on the surface of the skin that causes impetigo. Treatment therapy for impetigo can be done by administering natural medication from papaya seeds (Carica papaya L). This study aims to determine the antibacterial activity of Staphylococcus aureus papaya seed extract based on the different types of extract solvents used. This research is true experimental research with a posttest only control design research form. The research method used was the disk diffusion method with three treatments of papaya seed extract, namely 96% ethanol extract, ethyl acetate and n-hexane, positive control using chloramphenicol and negative control using 96% ethanol, ethyl acetate and n-hexane. The research results showed that each papaya seed extract treatment was able to inhibit Staphylococcus aureus with an inhibition zone of 96% ethanol extract of 18.09 mm, ethyl acetate extract of 7.93 mm, n-hexane extract of 0.36 mm. The One Way Anova test showed a value of ρα(0.05) so that there was a significant difference in the diameter of the inhibition zone in each extract treatment. The Tukey test showed that there were significant differences in the inhibition zone of Staphylococcus aureus bacteria (ρα(0.05)) in almost all treatments. The conclusion of this research is that there is a difference in the antibacterial activity of 96% ethanol extract, ethyl acetate extract and n-hexane extract of papaya seeds on the growth of Sthaphylococcus aureus bacteria.
Identification of Fungus Causing Onychomycosis in Chicken Meat Traders in Traditional Markets Mayumi, Ni Kadek Sintya; Habibah, Nur; Suyasa, I Nyoman Gede
JST (Jurnal Sains dan Teknologi) Vol. 12 No. 1 (2023): April
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v12i1.49203

Abstract

Onikomikosis merupakan infeksi pada kuku yang disebabkan oleh jamur dermatofita, non-dermatofita, dan yeast. Infeksi ditandai dengan perubahan warna dan penebalan lempeng kuku yang dapat bersifat kronis sehingga mengakibatkan kerusakan kuku. Jamur penyebab onikomikosis lebih mudah berkembang biak pada lingkungan yang kotor, lembab dan basah, sehingga orang yang bekerja pada lingkungan tersebut lebih beresiko mengalami onikomikosis. Kondisi pasar tradisional yang kotor, lembab dan basah merupakan salah satu lingkungan yang beresiko terhadap pertumbuhan dan perkembangbiakan jamur. Penelitian ini bertujuan untuk mengidentifikasi jamur penyebab onikomikosis pada kuku tangan pedagang daging ayam di kawasan Desa Batubulan. Sampel dalam penelitian ini diambil dari 30 orang pedagang daging ayam yang berasal dari lokasi 3 pasar tradisional di kawasan Desa Batubulan, Gianyar, Bali. Data dalam penelitian ini diperoleh dari wawancara, observasi serta pemeriksaan laboratorium untuk mengidentifikasi jenis jamur patogen penyebab onikomikosis. Identifikasi dilakukan dengan pemeriksaan kultur pada media Saboraoud Dextrosa Agar (SDA). Berdasarkan hasil yang diperoleh, diketahui bahwa seluruh media sampel (30) ditumbuhi oleh koloni jamur. Pemeriksaan dilanjutkan dengan pemeriksaan mikroskopis untuk mengidentifikasi koloni jamur yang tumbuh dengan pewarnaan Lactophenol Cotton Blue. Hasil identifikasi menunjukkan bahwa terdapat 18 jamur Aspergilus sp.  yang termasuk ke dalam golongan non-dermatofita, 10 jamur Trichophyton sp. yang termasuk dalam golongan dermatofita dan 10 jamur golongan yeast.  Berdasarkan hasil penelitian, diketahui bahwa jamur golongan non-dermatofita merupakan golongan jamur yang paling banyak ditemukan pada penelitian ini.
DESCRIPTION OF URIC ACID LEVELS BUS DRIVER PT ARSA BUANA MANUNGGAL KARANGASEM Yulia, Ni Putu Mentari; oka suyasa, ida bagus; sudarmanto, i gede; suyasa, i nyoman gede
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3209

Abstract

Uric acid is the end result of breaking down a substance called purine. Purine substance is a natural substance which one of the chemical structure groups is the formation of DNA and RNA. Uric acid can be influenced by several factors such as age, long sitting while driving, consumption of alcoholic beverages, and consumption of foods high in purines. The aim of this study was to determine uric acid levels in PT.ABM drivers. The study sample consisted of 45 people selected by random sampling using the POCT method with the Autoclick Easy Touch GCU tool. The results showed normal uric acid levels in 32 people (71.1%) and high uric acid levels in 13 people (28.9%). High uric acid levels were more common at the age of 43-51 years in 5 people (45.5%), duration of sitting while driving was dominated by driving duration for ? 7 hours/day in 10 people (29.4%), while drivers 11 people (34.4%) consumed alcoholic beverages and 25 people (55.6%) consumed high-purine foods. The conclusion of this study is that high uric acid levels occur in respondents aged 43-51 years, driving for ? 7 hours/day, consuming alcoholic beverages and consuming foods high in purines.
Kadar Debu Terhirup Menurunkan Kapasitas Vital Paru-Paru Tenaga Kerja Iron Candle Sudarmanto, I Gede; Purna, I Nyoman; Suyasa, I Nyoman Gede; Jirna, I Nyoman; Posmaningsih, D.A.A
Jurnal Kesehatan Lingkungan (JKL) Vol 12, No 2 (2022)
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jkl.v12i2.2220

Abstract

Dust particles are a collection of compounds in the form of solid or liquid which are scattered in the air with a very small diameter, less than 1 micron to a maximum of 500 microns. The size of dust particles that are harmful to health generally ranges from 0.1 microns to 10 microns. The dust particles will be in the air for a relatively long time in a hovering state. The work of crafting candle holders (iron candles), in the production process, allows for accidents and occupational diseases to arise from the stages of the manufacturing process. At the stage of the production process in the sanding room, a lot of dust is produced. There are workers in this section when working, some use masks and some do not. Even those who use masks are carried out infrequently, if by chance there is an inspection from the Regional Government or from the local Ministry of Manpower. The work environment is classified as hazardous to the health of the workers because dust particles can enter the body through the respiratory tract. A case control study has been conducted on 48 male and female workers at four iron candle craft companies in Tabanan district for 6 months, which is divided into 24 case groups and 24 control groups taken by cluster random sampling. The variables measured were the level of inhaled dust and the vital capacity of the workers' lungs. Analysis with the t-Paired test showed that there was a high causal relationship (significance) between the level of dust inhaled in the sanding work environment of the iron candle craft business in Tabanan district and the decrease in vital lung capacity of male and female workers.Keywords: Dust Content, Lung Vital Capacity. 
Efektifitas Pendidikan Kesehatan Dengan Media Booklet Terhadap Peningkatan Partisipasi Masyarakat Pada Bank Sampah Di Kabupaten Badung Suyasa, I Nyoman Gede; Sudarmanto, I Gede; Purna, I Nyoman
Jurnal Kesehatan Lingkungan (JKL) Vol 13, No 2 (2023)
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jkl.v13i2.2315

Abstract

Waste dumps are affected by overcrowding and human activity. Badung Regency produces 250 tons of garbage per day, to handle the waste problem Badung District Government is one of its efforts to establish a waste bank. This garbage bank was formed in the neighborhood of banjar, villages, schools and private institutions. The purpose of the study was to find out the increase in community participation in the waste bank program before and after health education with the media booklet. This type of research is pre-experimental research with one group pre-test-post-test design. The study population is the head of the family in Badung Regency. The sample size calculation obtained a sample of 337 people. Sampling with cluster random sampling, data collected using a questionnaire. Data collection before treatment and one month after treatment. Data analysis with paired t-test statistics. The results obtained by the level of community participation in waste banks before health education with the media booklet is very less 106 people (31.45%), less 14 people (4.15%), 40 people enough (11.87%), both 105 people ( 31.16%) and very good 72 people (21.36%). The level of community participation in the waste bank after health education with the media booklet is 31 people (9.20%), 175 people (51.93%) and very good 131 people (38.87%). And there are differences in community participation in waste banks before and after health education with a media booklet with a significance of 0,000. It is expected that the management of the official waste bank related to socialize the waste bank program to the public with the media booklet, facilitate prices to be stable and prepare transportation and personnel to take garbage to the homes of residents.
PENDAMPINGAN PASAR TRADISIONAL AMERTHA SEDANA DESA ADAT MENGWITANI KECAMATAN MENGWI KABUPATEN BADUNG MENUJU PASAR SEHAT Suyasa, I Nyoman Gede; Rusminingsih, Ni Ketut
Jurnal Pengabmas Masyarakat Sehat Vol 5, No 1 (2023): JANUARI
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jpms.v5i1.2300

Abstract

Amertha Sedana traditional market is a market managed by the Mengwitani Traditional Village with an area of approximately 43 acres, located on the Denpasar-Gilimanuk route. This market is open every day from 04.00 – 11.00 WITA in the morning and in the afternoon it is open from 15.00 to 22.00 WITA. Based on preliminary information, the traders who occupy the 2 main buildings consist of 42 kiosks, 71 stalls and 15 seasonal traders and 22 sengol traders. Priority problems that need to be solved Increasing knowledge of traders and visitors through health education with booklet media and giving masks to traders and visitors. Addition of temporary garbage shelters at each end of the stall. Addition of portable handwashing stations and hand sanitizers in strategic places before visitors and traders enter the market. The service method is by observing the Amertha Sedana traditional market, Mengwitani Traditional Village. Conduct an assessment before the service treatment is carried out on the health level of the traditional market. After the service is carried out, an assessment of the health of the market is carried out again. The target audience is managers, traders and visitors to traditional markets with working procedures. Prior to the assistance, a healthy market was infected using the Indonesian Ministry of Health's Decree No. 519/Menkes/SK/VI/2008 on traditional markets in Mengwi District. After the infection has identified the market sanitation problems that are still lacking, then assistance is provided to managers, traders and visitors. Furthermore, the level of market infection is measured after mentoring is carried out. The health level of Amertha Sedana Traditional Market in terms of market location, market buildings, sanitation, PHBS, security and other facilities in the market is generally healthy. Before each mentoring there was a clean and healthy living behavior of traders and penjung who were still not healthy, after mentoring by providing booklets, sinks and masks there was a change in clean and healthy living behavior into the behavior of traders and visitors in the healthy category. The total value of the Mengwi market health score is 6571 including healthy after mentoring the Amerta Sedana market health score in the Mengwitani Traditional Village to 7436 so that there is an increase of 865
PENGARUH PENGGUNAAN EKSTRAK BUNGA PACAR AIR (Impatiens balsamina L.) SEBAGAI ANTISEPTIK TERHADAP ANGKA KUMAN PADA TELAPAK TANGAN MAHASISWA POLTEKKES DENPASAR Pradnyani Putri, Dewa Ayu; Suyasa, I Nyoman Gede; Widhya HS, Cok Dewi; Suyasa, Ida Bagus Oka
Jurnal Skala Husada : The Journal of Health Vol 21, No 1 (2024): Jurnal Skala Husada (JSH)-The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v21i1.3314

Abstract

Pacar air flowers (Impatiens balsamina L.) hold potential as natural antibacterial agents, rich in compounds like flavonoids, alkaloids, steroidal saponins, tannins, quinones, and phenols. Pacar air flowers are utilized in traditional medicine for various medical conditions, including antibacterial properties. The purpose of this study is to determine the effect of using balsam flower extract (Impatiens balsamina L.) as a natural antiseptic in reducing total plate count on the hands of Poltekkes Denpasar students. Sterile hand swab samples were collected using a Pretest-Posttest Control Group Design to assess differences before and after the application of various concentrations. Statistical analysis was performed using One-way ANOVA. The study revealed differences in total plate count before and after the application of pacar air flower extract (Impatiens balsamina L.) on the hands of Poltekkes Denpasar students. The mean total plate count before treatment was 29,98 CFU/cm2, while after treatment, it was 4,70 CFU/cm2. Total plate count significantly decreased (p0.05) after applying the pacar air extract compared to before (Impatiens balsamina L.). There was a significant reduction in total plate count with the application of extract concentrations of 5%, 10%, and 15%