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Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak Daun Kopi Arabika (Coffea arabica) Menggunakan Teknik Kromatografi Nurul Amaliya Hikma; A. Nur Afiah Rahman; Siti Aisyah Basir; A. Yeyen Maharani; Pingki Pingki; A. Putri Wulandari Syam; Mufidah Humairah S; Nur Andini; Fatma Fatma; Fityatun Usman
Inovasi Kesehatan Global Vol. 2 No. 3 (2025): Agustus : Inovasi Kesehatan Global
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/ikg.v2i3.2216

Abstract

This study aims to extract and purify secondary metabolite compounds from Coffea arabica leaves using various separation techniques, such as maceration, column chromatography, thin layer chromatography (KLT), and preparatory KLT, and followed by flavonoid content analysis using UV-Vis spectrophotometry. The process begins with drying the arabica coffee leaves, then extraction is carried out using 96% ethanol for three days. After that, the solvent is evaporated with a rotary evaporator at 60°C to obtain a viscous extract. The extracts were further separated using column chromatography with a mixed solvent of n-hexane: ethyl acetate (3:1), resulting in 12 fractions that showed color differences, which was an indication of the diversity of the compounds in the extract. Advanced identification was carried out with KLT using methanol solvents: chloroform (9:1) at UV wavelengths of 254 nm and 366 nm, indicating the presence of compounds with medium to high polarity. Furthermore, the isolated compounds were further separated using the preparative KLT (KLTP) method. To determine the flavonoid content, UV-Vis spectrophotometry was performed at a wavelength of 431 nm, using quercetin as standard. The results of the analysis showed that Arabica coffee leaf extract had a yield of 7.35% and a total flavonoid content of 19.0605 mgQE/g. These findings show that arabica coffee leaves are a source of flavonoid compounds that have the potential for the development of herbal or phytopharmaceutical products. The flavonoid content found may provide health benefits, particularly in increasing antioxidant activity, which can be used in the treatment of oxidative stress-related diseases. The results of this study also open up opportunities for the development of plant-based products, especially Arabica coffee, in the pharmaceutical and cosmetic industries as an efficacious natural active ingredient.
Dampak Implementasi Merdeka Belajar Program Kampus Mengajar Di Wilayah 3T Indonesia Nur Andini; Maria Marsela
Journal Education for All: Media Informasi Ilmiah Bidang Pendidikan Luar Sekolah Vol 13 No 2 (2024): December-Journal Education For All
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jefa.v13i2.65361

Abstract

Penelitian ini bertujuan untuk membangun motivasi kewirausahaan masyarakat sebagai upaya strategis dalam meningkatkan perekonomian melalui pendidikan nonformal. Metode yang digunakan dalam penelitian ini adalah kajian literatur, yang menganalisis berbagai jurnal dan buku sebagai sumber utama data. Pengumpulan data dilakukan dengan menelaah sumber-sumber relevan yang mendukung analisis teoritis. Hasil penelitian menunjukkan bahwa motivasi kewirausahaan masyarakat dipengaruhi oleh minat, kesadaran akan peluang usaha, dan dukungan pendidikan nonformal. Selain itu, penelitian ini mengidentifikasi berbagai kendala yang dihadapi masyarakat dalam memulai dan mengembangkan usaha, seperti keterbatasan akses modal, keterampilan, dan jaringan pemasaran. Evaluasi terhadap peran pendidikan nonformal mengungkapkan pentingnya program-program pelatihan yang berbasis kebutuhan lokal untuk meningkatkan kemampuan kewirausahaan masyarakat secara berkelanjutan. Temuan ini diharapkan dapat menjadi landasan untuk pengembangan kebijakan dan program pendidikan nonformal yang lebih efektif dalam mendukung pemberdayaan ekonomi masyarakat.