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Journal : BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains

Efektivitas Salep Ekstrak Etanol Cengkeh (Syzygium aromaticum L) dalam Menghambat Pertumbuhan Bakteri Penyebab Infeksi Kulit Nabila, Vara; Nasution, Rizki Amelia; Mayasari, Ulfayani
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13002

Abstract

This study aims to determine whether clove ethanol extract can be formulated into ointment preparations, evaluate the effectiveness of the ointment in inhibiting bacterial growth causing skin infections, and identify the effective concentration of clove ethanol extract ointment formulation against Streptococcus pyogenes and Pseudomonas aeruginosa. The methods used were disk diffusion and well diffusion with clove ethanol extract concentrations of 10%, 20%, and 30%. Chloramphenicol served as a positive control, and distilled water as a negative control. The ointment formulation was tested at effective doses of 10% and 30% as per Pharmacopeia guidelines. The positive control used bactoderm ointment, and the negative control was an ointment base without clove extract. The results showed that clove ethanol extract could be formulated into an ointment after undergoing phytochemical screening. The clove ethanol extract ointment effectively inhibited bacterial growth causing skin infections. The inhibition zones for Streptococcus pyogenes were 12.16 mm and 14.06 mm, while for Pseudomonas aeruginosa they were 10.16 mm and 9.3 mm. The effective concentrations of clove ethanol extract ointment in inhibiting bacterial growth were 10% and 30%. Keywords: Bacteria, Skin Infection, Clove Ointment
Formulasi Sediaan Sheet Mask Ekstrak Daun Murbei (Morus alba L.) sebagai Anti Acne Terhadap Bakteri Propionibacterium acnes Harahap, Nurul Aina; Mayasari, Ulfayani; Nasution, Rizki Amelia
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13000

Abstract

This study aimed to determine the antibacterial activity of mulberry leaf extract and formulate Sheet Masks containing the extract at different concentrations of 4%, 6%, 8%, and 10%. The method involved antibacterial activity testing using the disk diffusion method with positive control (clindamycin and Wardah Sheet Mask), negative control (DMSO), and three replications. Organoleptic and physical evaluations of the Sheet Mask formulation were also conducted. The results showed that mulberry leaf extract inhibited the growth of Propionibacterium acnes, with inhibition zones of 6.0 mm at 4%, 9.45 mm at 6%, 12.57 mm at 8%, and 13.95 mm at 10%. For the Sheet Mask formulations, the inhibition zones were 10.69 mm for the positive control (clindamycin), 0.1 mm for F0, 9.78 mm for F1 (8%), and 10.29 mm for F2 (10%). Organoleptic evaluation revealed that the most preferred formulations in terms of color, aroma, and texture were F0, F2, and F1, respectively. Physical evaluation confirmed that the Sheet Mask formulations met quality standards. In conclusion, mulberry leaf extract demonstrated antibacterial activity against Propionibacterium acnes, with the most effective concentrations being 8% and 10%. Keywords: antibacterial, acne, Sheet Mask, mulberry, Propionibacterium acnes