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All Journal Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal Journal of Applied Veterinary Science and Technology
Lesmana, Muhammad Arfan
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

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Efficacy of Oregano Extract Ointment on Fibroblast Cells and Epidermis in Albino Rats with Excisional Wound Model Lesmana, Muhammad Arfan; Amri, Indah Amalia; Cahyanissa, Ardilla Rachma; Geoputri, Kartika Myrnandari; Anisa, Agri Kaltaria; Hardian, Andreas Bandang
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.262-270

Abstract

This study aimed to determine the effect of oregano extract ointment therapy on the number of fibroblast cells and the epidermal thickness in excised wounds. The experimental animals used were 20 male Wistar strain albino rats, 12 weeks old, weighing 100–150 g, divided into 5 treatment groups i.e., (C-) not excised and without ointment, (C+) excised and without ointment, and the treatment group were excised using a 5 x 5 mm biopsy punch and treated with oregano extract ointment using concentrations of (T1) 3%, (T2) 6% and (T3) 9% twice daily for 14 days in an excised wound. The variables observed in this study were the number of fibroblast cells and the epidermal thickness by the HE staining method then measured using ImageJ. Data analysis used the One-Way ANOVA test followed by the Tukey test (p < 0,05). The results showed a decrease in the number of fibroblast cells and an increase in the epidermal thickness in the 6% concentration ointment. This study concluded that the administration of oregano extract ointment with a concentration of 6% was the optimal concentration in accelerating the final proliferative phase, characterized by a decrease in fibroblast cells and an increase in epidermal thickness.
Effect of Artemisia vulgaris Supplementation on Zebrafish Embryo Under Heat Stress Condition during In Vitro Culture Tuska, Habib Syaiful Arif; Khamalt, Nursalsabila; Lesmana, Muhammad Arfan; Yesica, Reza; Hendrawan, Viski Fitri; Budiono; Residiwati, Gretania
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.20-25

Abstract

Background: Artemisia vulgaris contains flavonoids, which play a vital role in counteracting free radicals. Purpose: To determine the effect of Artemisia vulgaris extract supplementation on embryo development, heart rate and survival of zebrafish under heat stressed and non-heat stressed conditions. Methods: The research used a completely randomized design. Zebrafish embryos (n=240) were divided into heat stressed (36°C) and non-heat stressed (28°C) groups, while for each group were divided into three subgroups, namely T1/control (without Artemisia vulgaris supplementation); and supplemented group T1 and T2, with 2 µL and 4 µL of Artemisia vulgaris supplementation, respectively. The efficacy of Artemisia vulgaris supplementation was determined by observing the embryo development, heart rate, and survival rate of zebrafish up to 96 hours post fertilization (hpf). Results: The development of zebrafish embryos under heat stressed treated with Artemisia vulgaris extract gave the same quality as the control treatment without heat stressed exposure. Zebrafish embryos exposed to heat stressed with 4 µL Artemisia vulgaris supplementation gave the highest survival rate on the heat stressed group. Artemisia vulgaris supplementation improved the heart rate of zebrafish exposed to heat stressed as in the non-heat stressed group. Conclusion: Artemisia vulgaris extract can reduce the detrimental effects of heat stressed induction on zebrafish embryos, as evidenced by the improvement in embryonic development, heart rate, and survival rate of zebrafish embryos after supplementation.
Lycopene Supplementation Mitigates Heat Stress-Induced Impairment of Embryonic Development, Survival, and Heart Rate in Zebrafish (Danio rerio) Residiwati, Gretania; Nararya, Winandana Parama; Ainurrahma, Hafidha; Lonameo, Bonick Kartini; Lesmana, Muhammad Arfan; Agustina, Galuh Chandra; Budiono; Hartono, Djoko; Hendrawan, Viski Fitri; Tuska, Habib Syaiful Arif; Sidi, Shehu
Veterinary Biomedical and Clinical Journal Vol. 8 No. 1 (2026): Vol. 8 No. 1 2026
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2026.008.01.1

Abstract

Fish at any change in aquaculture air temperature can affect fish survival, physiological conditions and immune response. The need to study the reaction of fish to sublethal temperatures (30-40oC) is an impetus because of global warming which can trigger heat stress (HS). Heat stress (HS) and its effects can occur in fish with any changes in the environment (physical or physiological), where heat can change the animal's normal homeostasis. Tomato (Lycopersicum esculentum), has been proven as a fruit that is rich in lycopene, which acts as a natural antioxidant. The purpose of this study was to determine the effect of lycopene supplementation on the process of external fertilization on embryo development, survival rate and heart rate of zebrafish (Danio rerio) at normal temperature (NHS) and with heat exposure (HS). The survival rate data obtained will then be analyzed using SPSS, with ANOVA and LSD tests (P ≤0.05). While the results of the statistical analysis test of heart rate, a non-parametric test was used using Kruskal-Wallis H, to find out whether there was a significant difference in the treatment; and the Mann-Whitney Test, to detect significant differences between groups (P ≤0.05). The effect of lycopene supplementation on the external fertilization process of zebrafish embryos gives a low survival rate and heart vibration of zebrafish embryos, which is thought to be caused by several factors, namely zebrafish genetics; storage method, dosage, and lycopene extraction method used in the research
Co-Authors Agustina, Galuh Chandra Ainurrahma, Hafidha Amri, Indah Amalia Anisa, Agri Kaltaria Budiono Cahyanissa, Ardilla Rachma Geoputri, Kartika Myrnandari Hardian, Andreas Bandang Hartono, Djoko Khamalt, Nursalsabila Lonameo, Bonick Kartini Nararya, Winandana Parama Residiwati, Gretania Sidi, Shehu Tuska, Habib Syaiful Arif Viski Fitri Hendrawan Yesica, Reza