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Widyaswara, Gravinda
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Efektivitas sinar ultraviolet terhadap daya hambat pertumbuhan bakteri staphylococcus aureus dan Escherichia coli di laboratorium IMLTD Dagaina Koten, Rosadalima; Widyaswara, Gravinda; Rahman, Aulia; Rahmawati Zain, Kumara
Avicenna : Journal of Health Research Vol 8, No 2 (2025): OKTOBER
Publisher : STIKES Mamba'ul 'Ulum Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36419/avicenna.v8i2.1600

Abstract

Latar Belakang: Laboratorium IMLTD adalah laboratorium yang harus bebas dari kontaminasi bakteri. Bakteri Staphylococcus aureus dan Escherichia coli merupakan bakteri yang umum ditemukan di udara dan dapat hidup dalam tubuh manusia. Pertumbuhan kedua bakteri ini bisa dapat dikendalikan menggunakan sinar ultraviolet. Tujuan: Penelitian ini bertujuan untuk mengetahui efektivitas sinar ultraviolet terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli dengan periode waktu penyinaran 10, 20 dan 30 menit. Media yang digunakan untuk pertumbuhan bakteri adalah NA. Metode: Jenis penelitian yang dilakukan yaitu penelitian ekperimen dengan pendekatan cross sectional yaitu hasil penelitian memberikan perbandingan jumlah koloni yang tumbuh antara sampel kontrol dan sampel yang disinarai ultraviolet. Data yang diperoleh kemudian dianalisi secara deskriptif menggunakan microsoft excel. Hasil: Hasil yang diperoleh, pada kontrol tanpa penyinaran bakteri yang tumbuh >350 CFU. Setiap periode waktu paparan terhadap kedua bakteri mengalami penurunan jumlah koloni. Setiap periode paparan terjadi penurunan jumlah koloni. Penurunan jumlah bakteri paling banyak yaitu pada penyinaran 30 menit dengan jarak penyinaran 30 cm dan penurunan jumlah koloni paling sedikit yaitu pada penyinaran 10 menit dengan jarak 100 cm. Simpulan: Berdasarkan dari penelitian ini adalah sinar ultraviolet efektif dalam menghambat pertumbuhan bakteri. Periode waktu 30 menit jarak penyinaran 30 cm memberikan hasil yang efektif dibandingkan dengan periode paparan yang lain.
Efektivitas sinar ultraviolet terhadap daya hambat pertumbuhan bakteri escherichia coli dan bacillus subtilis di laboratorium seleksi donor Efiana Mida, Maria; Widyaswara, Gravinda; Tirtana, Arif; Rahmawati Zain, Kumara
Avicenna : Journal of Health Research Vol 8, No 2 (2025): OKTOBER
Publisher : STIKES Mamba'ul 'Ulum Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36419/avicenna.v8i2.1601

Abstract

Latar Belakang: Laboratorium seleksi donor merupakan laboratorium yang digunakan untuk menyeleksi calon pendonor dan harus bebas dari kontaminasi bakteri. Sterilisasi adalah proses membebaskan suatu alat, bahan dan media dari mikroorganisme bersifat patogen. Salah satu sterilisasi yang  digunakan adalah sterilisasi ultraviolet. Uji Efektivitas penyinaran sinar ultraviolet menggunakan media Nutrien Agar  dengan teknik streak  plate. Tujuan:  Mengetahui efektivitas hambatan pertumbuhan bakteri Escherichia coli dan Basillus subtilis pada waktu paparan sinar ultraviolet selama periode waktu 5, 10 dan 15 menit di laboratorium seleksi donor. Metode: Metode penelitian yang digunakan  yaitu True Eksperiment dengan pendekatan cross sectional. Data kemudian dianalisis secara deskritif menggunakan microsoft excel untuk melihat perbedaan jumlah koloni setelah disinari oleh ultraviolet. Hasil : Hasil yang didapatkan dari penelitian menunjukan bahwa kontrol tanpa penyinaran memiliki jumlah pertumbuhan bakteri  > 350 CFU. Hasil penelitian paparan sinar ultraviolet selama periode waktu 15 menit terhadap bakteri Escherichia coli dan Basillus subtilis menunjukan efektivitas dalam menghambat pertumbuhan bakteri, sementara penyinaran dengan periode  5 dan 10 menit masih terdapat banyak bakteri setelah dilakukan penyinaran. Simpulan : Kesimpulan paparan sinar ultraviolet efektif dalam menghambat pertumbuhan bakteri Escherichia coli dan Basillus subtilis pada periode 15 menit dibandingkan dengan durasi paparan selama 5 dan 10 menit.
HUBUNGAN KUALITAS TIDUR DENGAN KADAR GLUKOSA DARAH PADA WARGA KELAPA GADING DESA PURBAYAN Novita Sari, Ajeng; Sih Prabandari, Anggraeni; Widyaswara, Gravinda; Sundari, Evina
Avicenna : Journal of Health Research Vol 6, No 2 (2023): OKTOBER
Publisher : STIKES Mamba'ul 'Ulum Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36419/avicenna.v6i2.936

Abstract

Screening Parameters of Transfusion-Transmitted Infection using Rapid Diagnostic Method Widyaswara, Gravinda; Rahman, Aulia; Layyinatunnisa, Prilly; Sepe, Maria Regina; Koten, Rosadelima Dagaina; Tanzila, Zakia; Syofianis; Apu, Delia Irjayanti Tamu
Indonesian Journal of Biology Education Vol. 7 No. 1 (2024): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/ijobe.v7i1.1103

Abstract

Blood transfusions are an important part of medicine for patient recovery. Therefore, the transfused blood must be free from viruses, bacteria, and even parasites that can be transmitted through blood transfusions. Human Immunodeficiency Virus (HIV/AIDS), Hepatitis C, Hepatitis B, and Syphilis are among the illnesses that can be transferred through blood transfusions. This research aims to determine screening parameters for transfusion-transmitted infection using the rapid diagnostic test method. This type of research is experimental with a cross-sectional design. The samples used were ten blood samples, taken intravenously and then centrifuged at a speed of 1300 rpm for 4 minutes. After that, screening is carried out with a rapid diagnostic test. The screening results of the ten blood samples examined were valid and negative or non-reactive for HIV/AIDS, Hepatitis C, Hepatitis B, and Syphilis. These results indicate that the blood samples tested can be categorized as blood samples that passed the screening test or the blood examination test for four parameters of transfusion-transmitted infection.
Bacterial Contamination of Medical Scissors and Clamps in the Blood Collection Room Widyaswara, Gravinda; Tulit Ina, Maria Selviana; Tirtana, Arif; Zain, Kumara Rahmawati
MAXIMUS: Journal of Biological and Life Sciences Vol. 3 No. 2 (2025): October 2025
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/maximus.v3i2.2395

Abstract

Background: Bacterial contamination in blood products can originate from the donor's body or the surrounding environment. Contamination may occur during the blood collection process. Transmission typically happens through the hands and body of the medical personnel, contaminated clothing, medical instruments, and the surrounding environment. Bacterial contamination is a highly influential parameter in indoor air populations, especially in healthcare environments. Objective: The objective of this study was to identify the presence and growth of bacteria on medical scissors and clamps in the blood collection room of the Indonesian Red Cross Sleman Regency. Method: This research employed a descriptive observational method to analyze bacterial growth on scissors and clamps collected from the blood collection room of Indonesian Red Cross Sleman Regency. A cross-sectional approach was used, in which data on potential risk factors were collected at a single point in time. Nutrient Agar (NA) was used as the culture medium in this study. Result: The results of the bacterial isolation indicated that the scissors and clamps in the blood collection room of the Indonesian Red Cross Sleman Regency can be considered sterile, as the number of bacterial colonies did not exceed the normal threshold of <30 colonies. Specifically, 2 bacterial colonies were found on the scissors and 2 on the clamps. Conclusion: The presence of only 2 colonies each on the scissors and clamps suggests that the instruments in the blood collection room of Indonesian Red Cross Sleman Regency are within acceptable sterilization standards.
Co-Authors Adyana, Angga Alfinancy Ayulisa Keytimu, Elisabeth Alverina, Yohana Thania Xena Apu, Delia Irjayanti Tamu Aulia Rahman Dagaina Koten, Rosadalima Efiana Mida, Maria Hae, Shinny Love Laga Hayatillah, Raudhah Kodo, Jemi Arivitriyanto Livingston Koten, Rosadelima Dagaina Kumara Rahmawati Zain Layyinatunnisa, Prilly Martuti, Dian Novita Sari, Ajeng Oematan, Mershy Pebriana, Relita Rozikin, Rozikin Sari, Ajeng Novita Sarti, Rosaliana Sepe, Maria Regina Sepvianti, Wiwit Sih Prabandari, Anggraeni Siti Arifah sunartono sunartono Sundari, Evina Syofianis Tanzila, Zakia Tirtana, Arif Tri Wahyu Setyaningrum, Tri Wahyu Tulit Ina, Maria Selviana Widie Kemala Hapsari, Widie Kemala Zain, Kumaha Rahmawati