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All Journal Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) BIOMA : Jurnal Biologi Makassar Jurnal Insan Farmasi Indonesia Empowerment: Jurnal Pengabdian Masyarakat Jurnal ADAM : Jurnal Pengabdian Masyarakat Jurnal Komunitas Farmasi Nasional
Athiah Masykuroh
Akademi Farmasi Yarsi Pontianak
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an Antioxidant Activities of Urena lobata L. Ethanolic Extract by DPPH Method: Uji Aktivitas Antioksidan Ekstrak Etanol Bunga Pulutan (Urena lobata L) Menggunakan Metode DPPH Athiah Masykuroh; Saufa Hidayati
Jurnal Komunitas Farmasi Nasional Vol. 3 No. 1 (2023): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

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Antioxidants are compounds that have a molecular structure that can provide electrons to free radical molecules and break free radical reaction chains. The purpose of this study was to determine the estimated content of secondary metabolites from Urena Lobata L. flowers ethanolic extract by phytochemical screening method and their antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazil) method. Determination of the antioxidant activity of Urena Lobata L. flowers was carried out by observing the absorbance measured at a maximum wavelength of 516.40 nm and extract concentrations of 20 ppm, 40 ppm, 60 ppm, 80 ppm, and 100 ppm and then calculating the IC50 value. The results of the phytochemical screening test showed that the ethanol extract of pulutan flowers contained phenols, alkaloids, flavonoids, terpenoids, tannins and saponins. The results of the antioxidant activity test showed an average IC50 value of 5.337 ppm means it was categorized as a very strong antioxidant.
PENENTUAN NILAI SPF (Sun Protecting Factor) EKSTRAK ETANOL DAUN KALAKAI (Stenochlaena palustris (Burm F.) Bedd) Weni Puspita; Heny Puspasari; Athiah Masykuroh; Rika Fitria
Jurnal Komunitas Farmasi Nasional Vol. 3 No. 1 (2023): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

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Tanaman Kalakai (Stenochlaena palustris (Burm F.) Bedd) adalah tanaman paku-pakuan yang tumbuh di daerah rawa gambut yang secara umum disebut lahan basah. Berdasarkan studi empiris daun dan batang digunakan oleh suku dayak untuk mengobati anemia, penambah ASI pada ibu menyusui, pereda demam, mengobati sakit kulit, dan juga sebagai pencuci perut. Daun kalakai muda memiliki kandungan metabolit sekunder yaitu flavanoid yang efektif sebagai antioksidan dan menangkal radikal bebas. Penelitian ini bertujuan mengetahui apakah ekstrak etanol daun kalakai muda (Stenochlaena palustris (Burm.F.) Bedd) memiliki aktivitas perlindungan tabir surya. Ekstrak etanol daun kalakai muda dibuat larutan induk dengan konsentrasi 1000 ppm, kemudian dilakukan pengenceran dengan variasi konsentrasi 50 ppm, 75 ppm, 100 ppm, 125 ppm, dan 150 ppm. Penentuan nilai SPF dilakukan dengan mengukur absorbansi dari ekstrak etanol daun kalakai muda menggunakan spektrofotometri UV-Vis dengan panjang gelombang antara ±290-320 nm. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kalakai muda dengan kategori proteksi ultra berpotensi sebagai tabir surya. Nilai SPF yang diperoleh pada tiap konsentrasi 50 ppm, 75 ppm,100 ppm, 125 ppm dan 150 ppm secara berturut-turut adalah 9,43 (proteksi maksimal); 9,47 (proteksi maksimal); 13,32 (proteksi maksimal); 18,25 (proteksi ultra) dan 21,22 (proteksi ultra).
ANTIBACTERIAL ACTIVITY OF SILVER NANOCOLLOID GEL BIOSINTHESIZED BY Alocasia macrorrhizos PLANT EXTRACT AGAINST Staphylococcus aureus : UJI AKTIVITAS ANTIBAKTERI GEL NANOKOLOID PERAK HASIL BIOSINTESIS MENGGUNAKAN EKSTRAK TANAMAN KELADI SARAWAK (Alocasia macrorrhizos (L.)) TERHADAP BAKTERI Staphylococcus aureus Athiah Masykuroh; Ika Ristia Rahman; Hairunnisa; Nine Okta Meliadlina; Putry Fajaria Hardiani
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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An antibacterial activity test has been conducted against Staphylococcus aureus bacteria on silver nanocolloids gel biosynthesized using Alocasia macrorrhizos plant extract which contains phenolic derivative compounds : polyphenols, flavonoids and cyanogenic glycosides. The AgNO3 concentration used in the silver nanocolloid biosynthesis process is 0.05 M;0,10 M and 0,15 M. The silver nanocolloids was diluted in the amount of 50% from the previous concentration and innovated into a gel form (formula I, II and III) then tested on Staphylococcus aureus bacteria. The measurement of of the clear zone formed around the disc paper was carried out to determine it’s antibacterial activity. Only the third variation meet the requirements of the physical evaluation test results as a gel form. All concentrations of silver nanocolloid gel preparations can inhibit the growth of Staphylococcus aureus bacteria as shown in observations obtained by the average diameter of the inhibition zone for formula I of 12.55 mm, formula II of 12.64 mm and formula III of 12.71 mm which is the zone of resistance formed was induced strong category (10-20 mm). The significance value with the One way ANOVA test is 0.000 (p<0.05), means that the average value of each variation is significantly different.
PENENTUAN NILAI SPF (SUN PROTECTION FACTOR) EKSTRAK ETANOL BUNGA PULUTAN ( Urena lobata L.): PENENTUAN NILAI SPF (SUN PROTECTION FACTOR) EKSTRAK ETANOL BUNGA PULUTAN ( Urena lobata L.) Masykuroh, Athiah; Dian Kartika Sari; Husnani
Jurnal Komunitas Farmasi Nasional Vol. 4 No. 1 (2024): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

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Abstract

Urena lobata L. are known contains secondary metabolites of alkaloids, terpenoids, phenols, flavonoids, saponins and tannins and have very strong antioxidant activity. This causes the ethanolic extract of Urena lobata L. flowers have the ability to ward off free radicals and UV radiation. This research aims was to find out whether the ethanolic extract of urena lobata L. flowers has Sun Protection Factor (SPF) activity and how much value it has. Determination of the SPF value was carried out by measuring the absorbance of the ethanolic extract of Urena lobata L. flowers with concentration of 4000 ppm using UV-Vis Spectrophotometry at 290-320 nm wavelength. The results showed that the ethanolic extract of Urena lobata L. flowers has SPF activity. The average SPF value obtained was 2.43, which is included in the weak category.
FORMULASI MOUTHWASH EKSTRAK ETANOL DAUN KESUM (Polygonum minus Huds.): bahasa indonesia Kartikasari, Dian; Athiyah Masykuroh
Jurnal Komunitas Farmasi Nasional Vol. 4 No. 1 (2024): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

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Kesum leaves (Polygonum minus Huds.) contain phenolic compounds, flavonoids, alkaloids, steroids, terpenoids, tannins, saponins and essential oils. This study aims to determine the quality of Mouthwash preparations from ethanol extract of kesum leaves (Polygonum minus Huds.) Ethanol extract of kesum leaves (Polygonum minus Huds.) obtained by extraction using maceration method with 70% ethanol solvent for 3x24 hours. Furthermore, the extract was formulated into mouthwash preparations with variations in the concentration of ethanol extract of kesum leaves, namely 0.5%, 1%, 1.5%. Quality evaluation of the moutwash preparation includes organoleptic, pH, specific gravity and viscosity. The results obtained are in the organoleptical test results in the form of a liquid, smelling a distinctive aroma of kesum leaves and menthol, 0.5% formulation R1 and R2 are brownish yellow, R3 is light yellow and in 1% formulation R1, R2, R3 are brownish yellow and in 1.5% formulation R1, R2 and R3 are solid yellow, sweet taste, slightly spicy and slightly bitter in formulas 0.5%, 1% and 1.5% in R1, R2 and R3. Clarity test on formulations 0.5%, 1%, 1.5% produced clear preparations. Viscosity test in the 0.5% formula is (2.33 ± 0.577) cPs, 1% and 1.5% formulas are (2 ± 1 cPs). Specific gravity test in the 0.5% formula is (0.86 ± 0.33), 1% formula is (1.02 ± 0.01), 1.5% (1.05 ± 0.02). The pH test in the 0.5% formula is (6.28 ± 0.20), 1% formula is (5.46 ± 0.27), 1.5% formula is (5.18 ± 0.03). So it can be concluded that the three formulas have.
Bioaktivitas Sediaan Gel Nanokoloid Perak Hasil Biosintesis Menggunakan Ekstrak Tanaman Keladi Sarawak (Alocasia macrorrhizos (L.)) Terhadap Bakteri Escherichia coli: Bioactivity of Silver Nanocolloid Gel Preparations Resulting from Biosynthesis Using Sarawak Caladium Plant Extract (Alocasia macrorrhizos (L.)) Against Escherichia coli Bacteria Masykuroh, Athiah; Evani, Costa Rica
Jurnal Komunitas Farmasi Nasional Vol. 4 No. 02 (2024): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

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Biological activity (bioactivity) test of silver nanocolloid gel biosynthesized by Alocasia macrorrhizos (L.) plant extract and AgNO3 solutions against Escherichia coli bacteria has been conducted. The gel preparation was made with silver nanocolloids as an active ingredient. The AgNO3 solution used at biosynthesis process was made in 3 concentration variations : 0.05 (F1); 0.10 (F2) and 0.15 M (F3). Antibacterial activity test was conducted by paper disc diffusion method. The measuring results was diameter of the clear zone formed around the paper disc : 11.90 mm (F1), 12.67 mm (F2) and 13.13 mm (F3) with a positive control of neomycin sulfate gel of 23.16 mm. Based on these results, it can be concluded that the silver nanocolloid gel has inhibitory power against Escherichia coli bacteria with strong category for all variations.
Inovasi Nanokoloid Perak Hasil Biosintesis Menggunakan Ekstrak Tanaman Keladi Sarawak Dalam Bentuk Gel : Uji Stabilitas Fisik Dan Aktivitas Antibakteri Terhadap Staphylococcus aureus Athiah Masykuroh; Dzawil Kamala, Sirna; Sintia Puspita, Rani
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Stability test of Physical and Antibacterial Activity against Staphylococcus aureus of the silver nanocolloid gel biosynthesized by Alocasia macrorrhizos plant extract has been conducted. The test was using cycling test method to estimate stability of the gel. The AgNO3 concentration used in the silver nanocolloid biosynthesis process is 0.05 M ; 0.10 M and 0.15 M. The nanocolloid was diluted 50% of the initial concentration and innovated into a gel form (formula I, II and III) then tested on physical evaluation and antibacterial activity against Staphylococcus aureus bacteria. The physical evaluation test include organoleptic, pH, homogeneity, viscosity, spreadability and hedonic test. The measurement of of the clear zone formed around the disc paper was carried out to determine it’s antibacterial activity. The results shows all variation’s value decreased at physical evaluation test but still meet the requirements except for viscosity test and shows decereased at antibacterial activity test against Staphylococcus aureus. Kata kunci : silver nanocolloids, gel, Alocasia macrorrhizos (L.), cycling test, staphylococcus aureus
Co-Authors Dian Kartika Sari Dian Kartikasari Dina Yuspitasari Dzawil Kamala, Sirna Evani, Costa Rica Fadli Fadli Hairunnisa Heny Puspasari Husnani, Husnani Ihsanul Arief Nadia Nia Nurulita Nadia Nia Nurulita Nine Okta Meliadlina Nita Abna Nita Abna Puspasari, Heny Puspita, Weni Putry Fajaria Hardiani Rahman, Ika Ristia Ratna Widyasari Rika Fitria Saufa Hidayati Sintia Puspita, Rani Winda Tahuhiddah