Article Per Year (5 Year)
p-Index From 2020 - 2025
1.686
P-Index
This Author published in this journals
All Journal Majalah Kedokteran Gigi Indonesia Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Journal of Indonesian Dental Association B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah MDJ (Makassar Dental Journal) Cakradonya Dental Journal Medical and Health Journal
Prihastuti, Christiana Cahyani
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 8 Documents
Search

 1 
Antimicrobial photodynamic therapy with erythrosine photosensitizer against immune response in chronic periodontitis model Rochmawati, Mutia; Kusuma, Maulidina Raihan; Maziyyah, Faiz; Naim, Cantika Nadrotan; Prihastuti, Christiana Cahyani; Satrio, Rinawati; Laksitasari, Anindita; Sari, Dwi Nur Indah; Ichsyani, Meylida
Majalah Kedokteran Gigi Indonesia Vol 9, No 2 (2023): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77084

Abstract

Chronic periodontitis is a progressive inflammatory disease of the supporting tissues of the teeth caused by dental plaque bacteria with a clinical sign of periodontal pockets. A Gram-negative bacterium that can trigger this inflammatory disease is Porphyromonas gingivalis. Antimicrobial photodynamic therapy with blue LED light irradiation and photosensitizer erythrosine can reduce the survival rate of P. gingivalis. This study aimed to determine the effects of antimicrobial photodynamic therapy (APDT) exposure with blue LED light irradiation and PS erythrosine on the number of macrophages, lymphocytes, and gingival fibroblasts in gingival tissue of Sprague Dawley rats as chronic periodontitis models. This study used a posttest-only control group design to examine 27 Sprague Dawley rats which were divided into P group (healthy rats), N group (untreated chronic periodontitis rats), and PDT groups (chronic periodontitis model given 1 mg/ml PS erythrosine and irradiated with blue LED light for 60 seconds). Cell observation of histologic preparations of rat gingival tissue with hematoxylin-eosin (H&E) staining was carried out on the 1st, 3rd, and 5th days. Histological preparations of gingival tissue with H&E staining was carried out on the 1st, 3rd, and 5th days. Statistical analysis used a one-way ANOVA and the Kruskal-Wallis test, continued with LSD and the Mann-Whitney post-hoc tests. Results showed significant difference in the mean of macrophages in the PDT group compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). The mean lymphocyte in the PDT group was significantly different from the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05), and significantly lower than that in the healthy group (p < 0.05) but only on the 3rd and 5th days. The mean fibroblast in the PDT group was significantly different compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). In conclusion, there were significant differences in the number of macrophages, lymphocytes, and fibroblasts in a chronic periodontitis rat model after treatment with APDT exposure with blue LED and erythrosine photosensitizer.
AKTIVITAS ANTIBAKTERI EKSTRAK TANGKAI Begonia multangula Blume TERHADAP PERTUMBUHAN Aggregatibacter actinomycetemcomitans Sari, Dwi Nur Indah; Prihastuti, Christiana Cahyani; Andini, Restian Febi; Gusri, Aldina; Ghani, Muhammad Hisyam; Nabila, Haidy Lailatun
Medical and Health Journal Vol 3 No 1 (2023): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2023.3.1.8594

Abstract

Periodontitis adalah penyakit gigi dan mulut yang paling sering ditemukan pada masyarakat dan dapat menyebabkan tanggalnya gigi. Salah satu bakteri penyebab periodontitis adalah Aggregatibacter actinomycetemcomitans. Perawatan periodontitis dilakukan dengan terapi mekanik (scaling and root planning) disertai terapi kimiawi (antibiotik dan obat kumur). Penggunaan terapi kimiawi yang berkepanjangan dapat menyebabkan resistensi bakteri, gangguan pengecapan dan perubahan warna gigi. Alternatifnya dapat digunakan bahan herbal yang memiliki daya antibakteri Tangkai Begonia multangula Blume di beberapa wilayah digunakan sebagai tanaman obat serta memiliki aktivitas antibakteri. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak tangkai Begonia multangula Blume terhadap pertumbuhan A. actinomycetemcomitans secara in vitro. Penelitian dilakukan secara eksperimental laboratoris dengan sampel A. actinomycetemcomitans ATCC 43718. Tangkai Begonia multangula Blume diekstraksi menggunakan metode maserasi dan dibuat 5 seri konsentrasi (3,12%, 6,25%, 12,5%, 25%, dan 50%). Uji antibakteri dilakukan metode difusi kertas cakram dengan kontrol positif Chlorhexidine gluconate 2% serta kontrol negatif DMSO 1%. Diameter zona hambat selanjutnya dianalisis statistic. Hasil penelitian menunjukkan bahwa aktivitas antibakteri ekstrak tangkai Begonia multangula Blume meningkat seiring peningkatan konsentrasi dengan aktivitas tertinggi pada konsentrasi 50% dengan zona hambat sebesar 20,33 mm (p<0,05). Simpulan dalam penelitian ini bahwa ekstrak etanol tangkai Begonia multangula Blume memiliki aktivitas sebagai antibakteri A. actinomycetemcomitans.
Aloe vera extract wound healing sheet and free-range chicken egg albumin (gallus domesticus) accelerate angiogenesis on gingival incision wound in rats Putri, Myrna Evana Amanda; Prihastuti, Christiana Cahyani; Rochmawati, Mutia; Rosyada, Alya Ghina; A'ziza, Wizni A'dila
Padjadjaran Journal of Dentistry Vol 35, No 1 (2023): March 2023
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no1.42775

Abstract

ABSTRACTIntroduction: Incision wounds are injuries caused by dental surgery procedures. Suturing is the gold standard for post-incision management, but it can cause patient discomfort. Tissue glue can be used for wound healing, but its carcinogenicity still needs to be investigated. A Wound Healing Sheet (WHS) made of Aloe vera extract and free-range chicken egg albumin is expected to be an alternative material. Angiogenesis is a highly observable part of wound healing, as newly formed blood vessels support nutrition, act as a scaffold for cells to migrate to the wound area, and play an essential role in wound healing. This study aimed to analyze the effect of WHS made from Aloe vera extract and free-range chicken egg albumin (Gallus domesticus) on the number of vascular that show the acceleration of healing in gingival incision wounds. Methods: Quasi experimental study with a total sample of 24 male rats (Rattus norvegicus Wistar strain) with gingival incision were divided into four groups, namely T1 (50% WHS treated group), T2 (100% WHS treated group, S (suturing treated group as positive control), and NC (negative control, without treatment). The treatment was performed throughout seven days. The data obtained were analyzed statistically using One-way ANOVA and Post Hoc LSD. Results: The highest vascular count was in the T2 Group (8+1.63); followed by T1 (7+1.60); S (7+ 1.49); and NC (4+1.41). Statistical analysis showed that there was a significant difference between groups S, T1, T2 with NC (p-value < 0.05); interestingly, there was no significant difference between S, T1, T2 (p-value > 0.05). Conclusion: Wound Healing Sheet made of Aloe vera extract and free-range chicken egg albumin (Gallus domesticus) can accelerate the angiogenesis process on gingival incision wound in rats.Keywords: wound healing, aloe vera, gallus domesticus, egg white, angiogenesis
Antibiofilm Activity of Secang Wood (Caesalpinia sappan) Ethanol Extract Against Cariogenic Streptococcus sanguinis HAIBAR, FERINA AYU MAHARANI; Prihastuti, Christiana Cahyani; Ichsyani, Meylida
Journal of Indonesian Dental Association Vol 8 No 1 (2025): May
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v8i1.1278

Abstract

Streptococcus sanguinis merupakan bakteri pioneer colonizers yang berperan dalam perkembangan biofilm penyebab penyakit karies gigi. Ekstrak etanol kayu secang memiliki potensi antibiofilm yang dapat dikembangkan sebagai terapi alternatif obat kumur untuk pencegahan karies gigi. Penelitian bertujuan untuk mengetahui aktivitas antibiofilm ekstrak etanol kayu secang (Caesalpinia sappan L.) terhadap Streptococcus sanguinis penyebab karies gigi. Aktivitas antibiofilm menggunakan dua uji, uji pertama degradasi biofilm dan uji kedua penghambatan biofilm lanjutan dengan MtP Assay dan pewarnaan kristal violet 1% yang densitas optiknya dibaca pada panjang gelombang 620 nm. Data hasil degradasi penelitian dianalisis menggunakan One-Way ANOVA dan post-hoc LSD. Hasil menunjukkan terdapat perbedaan bermakna seluruh konsentrasi ekstrak dengan kontrol negatif. Konsentrasi efektif degradasi biofilm yaitu konsentrasi 1,56 mg/mL dan konsentrasi efektifpenghambatan pertumbuhan biofilm pada konsentrasi 0,39 mg/mL. Simpulan dari penelitian ini adalah terdapat aktivitas antibiofilm ekstrak etanol kayu secang terhadap Streptococcus sanguinis penyebab karies gigi.
Aktivitas antibiofilm ekstrak etanol daun saga (Abrus precatorius) terhadap biofilm bakteri Aggregatibacter actinomycetemcomitans pada pengujian menggunakan MtPB assay: experimental laboratoris Putri, Annisa Liontyn Adies; Ichsyani, Meylida; Prihastuti, Christiana Cahyani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.57014

Abstract

Pendahuluan: Aggregatibacter actinomycetemcomitans merupakan flora normal pembentuk biofilm di rongga mulut. Peningkatan jumlah bakteri ini secara berlebihan dapat menyebabkan terjadinya periodontitis agresif dengan prevalensi mencapai 90% sehingga berperan penting dalam peningkatan keparahan periodontitis agresif secara cepat. Daun saga (Abrus precatorius) memiliki aktivitas farmakologis seperti antitumor, antidiabetik, antihelmintes, dan antimikroba dengan kandungan fitokimia berupa tanin, saponin, flavonoid, steroid, dan alkaloid. Penelitian bertujuan untuk menganalisis aktivitas antibiofilm ekstrak etanol daun saga terhadap biofilm                            A. actinomycetemcomitans berfokus pada aktivitas degradasi dan penghambatan pembentukan biofilm. Metode: Penelitian dilakukan dengan metode MtPB menggunakan pewarna kristal violet. Ekstrak diuji pada berbagai konsentrasi bertingkat yaitu 0,39, 0,78, 1,56, 3,125, 6,25, 12,5, 25, dan 50 (mg/mL) yang dibandingkan terhadap kontrol negatif DMSO 1% serta kontrol positif amoksisilin+metronidazol 30 µg/mL. Data yang didapatkan melalui desain penelitian posttest only control group design kemudian dianalisis menggunakan analisis statistik one-way ANOVA dan analisis regresi linier Hasil: Hasil uji degradasi dan penghambatan pembentukan biofilm menunjukkan aktivitas pada seluruh kelompok perlakuan dibandingkan dengan kontrol negatif (p≤0,05). Ekstrak etanol daun saga konsentrasi 25 mg/mL memiliki kemampuan degradasi sebesar 76,13% serta sudah mampu menyamai kemampuan degradasi kelompok kontrol positif (p>0,05) dan mulai dari konsentrasi 1,56 mg/mL sudah menghambat biofilm mulai dari 37,52% sehingga mampu menyamai dan konsentrasi melebihi kemampuan penghambatan pembentukan biofilm A. actinomycetemcomitans pada kelompok kontrol positif. Nilai Minimum Biofilm Eradication Concentration (MBEC50) terdapat pada konsentrasi 4,44 mg/mL sedangkan nilai Minimum Biofilm Inhibitory Concentration (MBIC50) pada konsentrasi 2,96 mg/mL. Simpulan: ekstrak etanol daun saga memiliki aktivitas antibiofilm terhadap bakteri A. actinomycetemcomitans.Antibiofilm activity of ethanol extract of saga leaf (Abrus precatorius) on Aggregatibacter actinomycetemcomitans Biofilm: experimental laboratorisIntroduction: Aggregatibacter actinomycetemcomitans is a normal flora that forms biofilms in the oral cavity. An excessive increase in the number of these bacteria can cause inflammation of the periodontal tissue called periodontitis. A. actinomycetemcomitans has the main virulence of leukotoxin which can weaken the immune cell response to pathogenic bacteria with a prevalence in aggressive periodontitis reaching 90% so it plays an important role in the rapid increase in the severity of aggressive periodontitis. Saga leaves (Abrus precatorius) have pharmacological activities such as antitumor, antidiabetic, antihelminthic, and antimicrobial with phytochemical content in the form of tannins, saponins, flavonoids, steroids, and alkaloids. The research aims to analyze the antibiofilm activity of ethanol extract of saga leaves against A. actinomycetemcomitans biofilms by looking at the degradation activity and inhibition of biofilm formation. Method: The research was carried out using the MtPB method using crystal violet dye. The extract was tested at various graded concentrations, namely 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, and 50 (mg/mL) which were compared to the negative control 1% DMSO and the positive control amoxicillin +metronidazole 30 µg/mL. Data obtained through the posttest only control group design were then analyzed using one-way ANOVA statistical analysis and linear regression. Results: The results of the degradation and inhibition of biofilm formation tests showed activity in all treatment groups compared to the negative control (p≤0.05). The ethanol extract of saga leaves at a concentration of 25 mg/mL was able to match the degradation ability of the positive control group (p>0.05) and starting from a concentration of 1.56 mg/mL was able to match and exceed the ability to inhibit A. actinomycetemcomitans biofilm formation in the positive control group. The MBEC50 value is found at a concentration of 4.44 mg/mL while the MBIC50 value is at 2.96 mg/mL. Conclusion: ethanol extract of saga leaves has anti-biofilm activity against the bacteria A. actinomycetemcomitans.
TEMUAN KLINIS PSEUDOMEMBRAN ORAL CANDIDIASIS PADA PASIEN GAGAL GINJAL KRONIK Jayanti, Irene Putri; Pamungkasari, Dewanti Intan; Prihastuti, Christiana Cahyani; Saksana, Rachmad Aji
Cakradonya Dental Journal Vol 14, No 2 (2022): Agustus 2022
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v14i2.27112

Abstract

Gagal ginjal kronik (GGK) merupakan penyakit ginjal dengan glomelural filtration rate (GFR) sekitar 15-59 mL/menit/1.73m. Manifestasi oral pada pasien GGK telah banyak dilaporkan. Manifestasi tersebut dapat dipengaruhi oleh penyakit ginjal itu sendiri, obat, maupun terapi yang diberikan. Laporan kasus ini bertujuan untuk menyajikan temuan klinis pseudomembran oral candidiasis pada pasien GGK. Seorang laki-laki berusia 41 tahun menderita GGK dan sedang dirawat inap di Bangsal Mawar, RSUD Prof. Dr. Margono Soekarjo. Pasien tersebut mengeluhkan bercak putih pada lidah dan mulut kering. Pasien jarang sikat gigi selama dirawat inap. Pemeriksaan intaoral terdapat lesi berupa plak berbentuk irregular berwarna putih di area dorsum lidah yang dapat dikerok dan meninggalkan area kemerahan. Rongga mulut pasien juga tercium adanya bau uremik. Pasien didiagnosis pseudomembran oral candidiasis yang diduga akibat kondisi xerostomia karena kadar ureum yang tinggi serta penggunaan obat antihipertensi (amlodipin) dan diuretik (furosemide). Xerostomia juga dapat dipengaruhi oral hygiene yang tidak terjaga selama rawat inap, kondisi imunosupresi, dan malnutrisi yang meningkatkan risiko terjadinya pseudomembran oral candidiasis. Pilihan medikasi untuk pseudomembran oral candidiasis yang aman bagi pasien GGK adalah nistatin sediaan krim atau suspensi oral. Kesimpulannya adalah GGK merupakan penyakit sistemik yang dapat mempengaruhi timbulnya lesi oral, antara lain pseudomembran oral candidiasis.Kata Kunci: Gagal ginjal kronik, pseudomembran oral candidiasis, xerostomia.
EFEKTIVITAS MIKROEMULSI-GEL SELEDRI (Apium graveolens) DALAM MENURUNKAN KADAR CALPROTECTIN GCF PADA MODEL PERIODONTITIS KRONIS Prihastuti, Christiana Cahyani; Salisa, Ziyada; Rakhmawati, Rakhmawati; Taqwim, Ali; Satrio, Rinawati
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 12, No 1 (2025): Volume 12 No 1, Juni 2025
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v12i1.1812

Abstract

Background: Calprotectin is a neutrophil secretion product that serves as one of the biomarkers for periodontal tissue inflammation. Calprotectin levels in Gingival Crevicular Fluid (GCF) are reported to increase in periodontitis conditions. Celery (Apium graveolens) contains flavonoids and polyphenols that have potential anti-inflammatory properties. The topical microemulsion-gel formulation offers optimal bioavailability of active substances when applied to periodontal pockets. Objective: To determine the effect of celery microemulsion-gel on GCF calprotectin levels in an experimental model of chronic periodontitis. Methods: This experimental study used 25 male Wistar rats (Rattus norvegicus) divided into 5 groups (n=5): healthy control group, negative control group, and three treatment groups receiving Apium graveolens microemulsion-gel at concentrations of 25%, 50%, and 100%. The chronic periodontitis model was induced using P. gingivalis. Apium graveolens microemulsion-gel (0.7 ml) was applied to the inflamed gingival sulcus for 7 days. GCF samples were collected using filter paper and calprotectin levels were measured using ELISA method. Data analysis was performed using One-Way ANOVA followed by Least Significant Difference (LSD) test. Results: GCF calprotectin levels in the Apium graveolens microemulsion-gel treatment groups were lower compared to the negative control group (p<0.05), with the lowest levels observed in the 50% concentration group. There was no significant difference between the treatment groups and the healthy control group (p>0.05). Conclusion: Apium graveolens microemulsion-gel effectively reduces GCF calprotectin levels in chronic periodontitis model, indicating an anti-inflammatory effect.
Clinical findings of median rhomboid glossitis in typhoid fever patients: Temuan klinis median rhomboid glossitis pada pasien demam tifoid Arista, Erlinda Bella; Cahyono, Luqman Mahadika; Nafsih, Thoharoh Laili; Prihastuti, Christiana Cahyani; Purnomohadi, Sigit
Makassar Dental Journal Vol. 14 No. 2 (2025): Volume 14 Issue 2 August 2025
Publisher : Makassar Dental Journal PDGI Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35856/mdj.v14i2.1249

Abstract

Typhoid fever is an acute infection of the gastrointestinal tract caused by Salmonella typhi. Oral manifestations that can appear include median rhomboid glossitis. This article reports a case of MRG in a patient with typhoid fever. A 40-year-old man with com-plaints of fever, mouth ulcers, nausea, vomiting, abdominal pain, diarrhoea, and blackish faeces. The diagnosis of typhoid fever was confirmed. Oral examination revealed a reddish parallelogram-shaped depapillation lesion in the centre of the tongue, which was sore and surrounded by a white coating that could be scraped off. MRG is an inflammation of the tongue characterised by erythema lesions on the median dorsum of the tongue caused by Candida infection. The finding of MRG oral lesions in this ty-phoid fever case is thought to be influenced by the condition of lymphocytopenia which causes a decrease in patient immunity, increasing the risk of opportunistic Candida infection in the oral cavity. In addition, there are also predisposing factors such as smoking habits and poor oral hygiene conditions. It is concluded that the management of MRG lesions is to provide information and education to patients to maintain oral hygiene, avoid consumption of hot and spicy foods and drinks that aggravate com-plaints on the tongue.
Co-Authors A'ziza, Wizni A'dila Ali Taqwim Andini, Restian Febi Arista, Erlinda Bella Cahyono, Luqman Mahadika Dwi Nur Indah Sari Ghani, Muhammad Hisyam Gusri, Aldina HAIBAR, FERINA AYU MAHARANI Ichsyani, Meylida Jayanti, Irene Putri Kusuma, Maulidina Raihan Laksitasari, Anindita Maziyyah, Faiz Mutia Rochmawati, Mutia Nabila, Haidy Lailatun Nafsih, Thoharoh Laili Naim, Cantika Nadrotan Pamungkasari, Dewanti Intan Purnomohadi, Sigit Putri, Annisa Liontyn Adies Putri, Myrna Evana Amanda Rachmad Aji Saksana Rakhmawati Rakhmawati Rosyada, Alya Ghina Salisa, Ziyada Satrio, Rinawati