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All Journal Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi) Journal of Indonesian Dental Association Mandala of Health : A Scientific Journal Medical and Health Journal
Laksitasari, Anindita
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Dentistry Health Professions Medicine & Pharmacology Public Health

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Antimicrobial photodynamic therapy with erythrosine photosensitizer against immune response in chronic periodontitis model Rochmawati, Mutia; Kusuma, Maulidina Raihan; Maziyyah, Faiz; Naim, Cantika Nadrotan; Prihastuti, Christiana Cahyani; Satrio, Rinawati; Laksitasari, Anindita; Sari, Dwi Nur Indah; Ichsyani, Meylida
Majalah Kedokteran Gigi Indonesia Vol 9, No 2 (2023): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77084

Abstract

Chronic periodontitis is a progressive inflammatory disease of the supporting tissues of the teeth caused by dental plaque bacteria with a clinical sign of periodontal pockets. A Gram-negative bacterium that can trigger this inflammatory disease is Porphyromonas gingivalis. Antimicrobial photodynamic therapy with blue LED light irradiation and photosensitizer erythrosine can reduce the survival rate of P. gingivalis. This study aimed to determine the effects of antimicrobial photodynamic therapy (APDT) exposure with blue LED light irradiation and PS erythrosine on the number of macrophages, lymphocytes, and gingival fibroblasts in gingival tissue of Sprague Dawley rats as chronic periodontitis models. This study used a posttest-only control group design to examine 27 Sprague Dawley rats which were divided into P group (healthy rats), N group (untreated chronic periodontitis rats), and PDT groups (chronic periodontitis model given 1 mg/ml PS erythrosine and irradiated with blue LED light for 60 seconds). Cell observation of histologic preparations of rat gingival tissue with hematoxylin-eosin (H&E) staining was carried out on the 1st, 3rd, and 5th days. Histological preparations of gingival tissue with H&E staining was carried out on the 1st, 3rd, and 5th days. Statistical analysis used a one-way ANOVA and the Kruskal-Wallis test, continued with LSD and the Mann-Whitney post-hoc tests. Results showed significant difference in the mean of macrophages in the PDT group compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). The mean lymphocyte in the PDT group was significantly different from the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05), and significantly lower than that in the healthy group (p < 0.05) but only on the 3rd and 5th days. The mean fibroblast in the PDT group was significantly different compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). In conclusion, there were significant differences in the number of macrophages, lymphocytes, and fibroblasts in a chronic periodontitis rat model after treatment with APDT exposure with blue LED and erythrosine photosensitizer.
POTENTIAL OF RED ONION PEEL ETHANOL EXTRACT (Allium cepa L.) TO DEGRADATION OF Staphylococcus aureus BIOFILM Sari, Dwi Nur Indah; Rosyada, Alya Ghina; Laksitasari, Anindita; Djati, Fanni Kusuma; Andini, Restian Febi
Medical and Health Journal Vol 3 No 2 (2024): February
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2024.3.2.11371

Abstract

Dental biofilm is a bacterial colonization adhering to the tooth surface, enveloped by an extracellular matrix. This biofilm shields bacteria from the body's defense and antibacterial systems, potentially leading to various dental and oral diseases.Staphylococcus aureus is among the bacteria forming dental biofilm. Red onion peel is known to contain flavonoids, saponins, tannins, steroids and alkaloids which have antibacterial and anti-biofilm activity. This study aimed to determine the potential of shallot peel extract in degrading S. aureus biofilms. This study was conducted with a posttest-only control group design. Red onion peel extraction is carried out using the maceration method. A total of 5 groups of extracts (50%, 25%, 12.5%, 6.25%, and 3.12%), positive control (chlorhexidine gluconate 0.2%), and negative control (DMSO 1%) were tested for their activity in S. aureus biofilm degradation at 24 and 48 hours of incubation. The biofilm degradation was assessed using the microtiter plate assay method with crystal violet staining read at a wavelength of 595 nm. Percent biofilm degradation was statistically analyzed using Two way ANOVA and LSD. The results indicated significant differences based on treatment, incubation time, and the interaction between the two. The highest activity was observed at a concentration of 25%, although it was still lower than that of positive control. Conclusion: the ethanol extract of red onion peel has the potential to degrade S. aureus biofilms, with the highest activity at a concentration of 25% and an incubation time of 48 hours.
Oral health literacy, knowledge, attitude, and oral health practices among college students at Jenderal Soedirman University Fitri Diah Oktadewi; Romdlon, Mahindra Awwaludin; Laksitasari, Anindita; Hidayah, Riski Amalia
Dental Journal (Majalah Kedokteran Gigi) Vol. 57 No. 4 (2024): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i4.p280-285

Abstract

Background: Oral health literacy (OHL) has an important role as a medium for health promotion and efforts to prevent oral diseases through oral health behavior changes. Purpose: This study aims to determine OHL and its correlation with oral health knowledge, attitude, and oral health practice among college students. Methods: This cross-sectional study was conducted on 583 students of Jenderal Soedirman University in 2020, as the students come from different provinces in Indonesia. The Indonesian Oral Health Literacy Questionnaire was used to assess OHL. A self-designed questionnaire was used to assess oral health knowledge, attitude, and oral health practice. Results: Most students had good OHL, knowledge, and attitude toward oral health (90.7%, 84.6%, and 90.5% respectively). A Pearson correlation test showed a correlation between OHL and oral health knowledge (p = 0.044) and attitude (p = < 0.001). The Fisher exact tests showed a correlation between OHL and snacking frequency, tooth-brushing frequency, tooth-brushing time, and toothbrush changing time. The better the OHL, the better the knowledge and attitude. There was no correlation between OHL and sweet food eating frequency, the use of cleansing aids, dentist visit frequency, and the first action taken when experiencing toothache. Conclusion: OHL has a correlation with oral health knowledge and attitude. However, OHL is only related to some oral health practice indicators. The results of this study are expected to be taken into consideration when formulating strategies to improve oral health within the university.
Atrophic glossitis and recurrent aphthous stomatitis in patients with anemia and abdominal tuberculosis Putri, Devi Anisya; Laksitasari, Anindita; Pramuditya, Hafizh Zufar; Silva, Ghea De
Journal of Indonesian Dental Association Vol 8 No 1 (2025): May
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v8i1.1208

Abstract

Pendahuluan: Anemia merupakan kondisi penurunan jumlah eritrosit dan kadar hemoglobin, yang dapat disebabkan oleh tuberkulosis (TB) abdomen melalui beberapa mekanisme. Tuberkulosis abdomen disebabkan bakteri Mycobacterium tuberculosis, melibatkan saluran pencernaan, peritoneum, mesenterika, hati, dan limpa. Penderita anemia sering mengalami masalah rongga mulut. Tujuan: Menguraikan manifestasi klinis dan manifestasi oral pada pasien anemia dan TB abdomen. Studi Kasus: Pasien laki-laki berusia 34 tahun dirawat inap di RSUD Margono Soekarjo mengeluhkan perut membesar, kembung, sulit buang angin dan buang air besar sejak 1 bulan. Nafsu makan menurun. Pasien didiagnosis tuberkulosis abdomen dan anemia. Terdapat lesi ulcer pada gingiva bukal rahang atas dan bawah, serta atrofi papilla lidah sehingga pasien didiagnosis stomatitis aftosa rekuren dan atrofik glossitis. Pembahasan: Anemia dapat disebabkan oleh tuberkulosis abdomen melalui perdarahan, gangguan penyerapan nutrisi, dan efek sistemik. Fungsi usus terganggu sehingga menghambat penyerapan nutrisi seperti zat besi dan vitamin B12 yang penting dalam pembentukan eritrosit. Penderita anemia sering mengalami masalah rongga mulut seperti stomatitis dan glossitis. Glossitis yaitu atrofi pada papilla dorsum lidah ditandai sensasi terbakar. Stomatitis aftosa rekuren dapat terjadi karena transportasi oksigen dan nutrisi terganggu sehingga menghambat diferensiasi dan pertumbuhan sel epitel. Kesimpulan: Anemia salah satunya dapat disebabkan oleh tuberkulosis abdomen dan menimbulkan manifestasi pada oral seperti glossitis dan stomatitis aftosa rekuren. Penegakan diagnosis yang baik dapat menentukan pengobatan yang tepat.
INHIBITION OF Asystasia gangetica ETHANOLIC EXTRACT ON Porphyromonas gingivalis AND Fusobacterium nucleatum BIOFILM FORMATION Ichsyani, Meylida; Laksitasari, Anindita; Supriyati, Supriyati; Sari, Dwi Nur Indah; Wahyudin, Wahyudin
Mandala Of Health Vol 18 No 1 (2025): Mandala of Health: A scientific Journal
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Periodontal pathogens Porphyromonas gingivalis and Fusobacterium nucleatum develop a biofilm on the surface of the dental apex, causing inflammation in the teeth' supporting tissues. Asystasia gangetica has been observed for its analgesic, anti-inflammatory, and antimicrobial effect. The ethanolic extract of A. gangetica may be developed as a root canal sterilization dressing due to its benefits. This study aims to determine the inhibitory effect of ethanolic extract of A. agangetica on P. gingivalis and F. nucleatum from forming biofilm. Antibacterial activity was determined using Kirby-Bauer disk diffusion. The inhibition of biofilm formation was evaluated using microtiter plate biofilm assay with various concentrations of extract. Base on the classification of inhibition zone, ethanolic extract of A. gangetica had moderate to strong antibacterial effects on P. gingivalis at 12.5-50 mg/mL, and moderate effect on F. nucleatum at 25-50 mg/mL. Results also showed that the extract at 50 mg/mL, inhibited biofilm formation by more than 72% against P. gingivalis (p<0.05) and up to 75% against F. nucleatum (p<0.05). The value of MBIC50 was determined at 5.19 mg/mL and 7.44 mg/mL, respectively. Ethanolic extract of Asystasia gangetica has a potential inhibitory effect on P. gingivalis and F. nucleatum biofilm formation. Studies suggest more detailed approaches to a better understanding of the interactions between lead compounds and bacterial cells within the biofilm structure.
KONSENTRASI EKSTRAK ETANOLIK DAUN PEPAYA (Carica papaya L.) TERHADAP PERUBAHAN WARNA BASIS GIGI TIRUAN RESIN AKRILIK POLIMERISASI PANAS Purnama, Ryana Budi; Laksitasari, Anindita; Handayani, Fani Tuti; Widyaningsih, Pratiwi Nur; Rahmawati, Dewi; Milla, Dzhofirotul; Artanti, Evania Bellinda; Nahya, Bintan
Mandala Of Health Vol 17 No 2 (2024): Mandala of Health: a Scientific Journal
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mandala.2024.17.2.12218

Abstract

Heat-cured acrylic resin is one of the materials used in making denture bases which has the disadvantage that it easily absorbs water and can make discoloration. Papaya leaves (C. papaya L) contain phenolic compounds (C6H6OH-) which can affect the color change of heat-cured acrylic resin. The purpose of this study is to determine the effect of various concentrations of papaya extract on discoloration of the heat-cured acrylic resin denture base. This experimental research is done in vitro at a laboratory using papaya leaf extract (C. papaya L) concentrations of 5%, 10%, and 20%. Thirty two samples of acrylic resin plates were divided into 4 groups, namely the immersion treatment group in papaya leaf extract concentrations of 5%, 10%, and 20%, and the negative control group in the form of aquadest soaking. The color change test was carried out using a UV-visible spectrophotometer. Data was analyzed using the One-Way ANOVA followed by Post-Hoc LSD test. The results of the study showed that there was a very significant difference in the mean value of color change between the treatment groups soaking in papaya leaf extract at concentrations of 10% and 20% and the control group, while at 5% concentration there was no significant difference from the control group. The lowest mean absorbance value was in the distilled water immersion group of 3.341350 ± 0.0942091 and the highest mean absorbance value was in the 20% immersion group of 3.553638 ± 0.0227829. This study concluded that there is an effect of various concentrations of papaya leaf extract on discoloration of the heat-cured acrylic resin denture base. Keywords: Carica papaya L, discoloration, acrylic resin, denture base.
Co-Authors Andini, Restian Febi Artanti, Evania Bellinda DEWI RAHMAWATI Dwi Nur Indah Sari Fani Tuti Handayani, Fani Tuti Fanni Kusuma Djati Fitri Diah Oktadewi Kusuma, Maulidina Raihan Mahindra Awwaludin Romdlon Maziyyah, Faiz Meylida Ichsyani Milla, Dzhofirotul Mutia Rochmawati Nahya, Bintan Naim, Cantika Nadrotan Pramuditya, Hafizh Zufar Prihastuti, Christiana Cahyani Purnama, Ryana Budi Putri, Devi Anisya Riski Amalia Hidayah Rosyada, Alya Ghina Satrio, Rinawati Silva, Ghea De Supriyati . Wahyudin Wahyudin Widyaningsih, Pratiwi Nur