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All Journal Jurnal Natur Indonesia Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Microbiology Indonesia Jurnal Bioleuser Jurnal AgroBiogen Elkawnie Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosaintifika: Journal of Biology & Biology Education Jurnal Entomologi Indonesia Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Biosaintifika: Journal of Biology & Biology Education JURNAL PENGABDIAN PEMBANGUNAN PERTANIAN DAN LINGKUNGAN (JP3L)
Lenni Fitri
Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Social Sciences

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DNA Extraction and PCR Optimization of Coffea arabica L. and Coffea canephora Pierre ex A. Froehner Ramlan, Risa Riani; Harnelly, Essy; Fitri, Lenni
Jurnal Penelitian Pendidikan IPA Vol 10 No SpecialIssue (2024): Science Education, Ecotourism, Health Science
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10iSpecialIssue.7881

Abstract

DNA isolation is an important step for further molecular analysis. Coffee plants contain polysaccharides, polyphenols, and secondary metabolites which can contaminate the results of DNA isolation. This study was conducted to isolate DNA from arabica and robusta coffee leaves using a modified CTAB method and PCR optimization for amplification of matK and rbcL genes. DNA was isolated using buffers (CTAB 10%, PVP 1%, β-mercaptoethanol 1%, Tris-HCl 1M, NaCl 5M, and EDTA 0.5%), and eluted with TE-RNase. Genomic DNA of ten coffee plants was successfully isolated with concentrations ranging from 33-146 nm/µL with purity (A260/A280) 1.7-1.9. Based on the genomic DNA isolated, matK and rbcL  genes were amplified with initial denaturation conditions of 94°C (for 1 minute of matK and 4 minutes of rbcL) followed by 35 cycles of denaturation at 94°C for 30 seconds, annealing (adjusted to each primer), and extension at 72°C for 1 minute. The optimal PCR conditions were effective to amplify matK (900 bp) and rbcL (600 bp) genes. Thus, the modified DNA isolation method and PCR optimization can be used as an efficient tool for further molecular analysis of Coffea arabica L. and Coffea canephora Pierre ex A. Froehner.
Secondary Metabolites Profiling and Antimicrobial Activities of Ethanol Extract from Jamblang (Syzygium cumini L.) Stem Bark Meutia, Fayza; Ismail, Yulia Sari; Fitri, Lenni
Jurnal Penelitian Pendidikan IPA Vol 11 No 5 (2025): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i5.10589

Abstract

This study was conducted to determine the secondary metabolite compounds and evaluate the antimicrobial activity of ethanol extract of jamblang stem bark (Syzygium cumini). The method used in this research is experimental method. The extraction was carried out using the maceration method with 96% ethanol as the solvent. Secondary metabolite compounds of the extract samples were analyzed using a Gas Chromatography-Mass Spectrometry (GC-MS). Antimicrobial activity of the extract against Cutibacterium acnes, Staphylococcus aureus, and Candida albicans using disc diffusion method tested on MHA (Mueller Hinton Agar) media with extract concentrations of 40%, 60%, 80%, and 100%.  Each experiment was repeated three times to ensure validity. The results revealed that the extract have dominant compounds such as 7-Tetradecenal (Z), Octadecanoic acid, and n-Hexadecanoic acid. The antimicrobial test showed that the highest inhibition zones were 7.45 mm for C. acnes, 8.04 mm for S. aureus, and 4.60 mm for C. albicans at 100% extract concentration. The inhibition zones increased with higher extract concentrations, indicating a dose-dependent relationship. Compared to the positive control results (20.70 mm; 22.29 mm; and 18.62 mm) the extract showed moderate antimicrobial activity. These findings suggest that jamblang stem bark extract has potential as a natural antimicrobial agent.
Co-Authors Amirunnas , Amirunnas Anja Meryandini Ariqah, Nada Aulia, Muhammad Rizqi Betty Mauliya Bustam Dhani, Desy Nurrahma DITA, SASMIATI FARACH Dyah Iswantini Essy Harnelly Eva Agustriana Fauziah Fauziah Firdus Firdus Fitria Ardani Ismail, Yulia Sari M. Nur Salim Meutia, Fayza Putriani, Putriani Putriani, Putriani Ramlan, Risa Riani Rauzana, Anita Rizki, Alia RUHUL KHALILA Safriani, Suri Raihan Sri Rahayu Suhartono Suhartono Suhartono Syafitri, Aini Warzatullisna, Warzatullisna Wira Dharma Yekki Yasmin Yekki Yasmin Yekki Yasmin Yekki Yasmin Yulia Sari Ismail Yulia Sari Ismail YULIN LESTARI Zahrina, Yuni Zakaria, Rahmad