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Journal : Journal of Pharmacy Science and Technology

Identifikasi Identifikasi Fitokimia Daun Gandarusa (Justicia Gendarussa L.) Asal Kabupaten Pangkep: Analisis Kandungan Senyawa Aktif Menggunakan Metode Kromatografi dan Uji Fitokimia Ildayani, Ildayani; Ramadhani, Livia Ernesta; Sa’adah, Nur Lailatus; Apriliani, Ayu; Natasya, Natasya; Ikram, Nurul; Mutiara, Sitti Ayzah; Sari, Andi Aliya Meilana; Sahruni, Sahruni; Usman, Fityatun; Haryanto, Haryanto
JOURNAL OF PHARMACY SCIENCE AND TECHNOLOGY Volume 5 No. 1: Juli 2024
Publisher : Prodi Farmasi Fakultas Kedokteran Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/pst.v5i1.395

Abstract

Gandarusa leaves (Justicia gendarussa L.) are recognized as medicinal plants rich in various active phytochemicals. Extraction was carried out using the maceration method with 96% ethanol, followed by evaporation with a rotary evaporator to obtain a concentrated extract. Fractionation was performed using liquid-liquid partitioning with n-hexane and ethyl acetate to separate compounds based on polarity. The results revealed the ethyl acetate fraction had the highest yield at 20.8%, followed by the n-hexane fraction at 20%. Phytochemical identification using thin-layer chromatography confirmed the presence of alkaloids, flavonoids, and steroids. UV-Vis spectrophotometry analysis showed the total flavonoid content reached 0.741 mg/L based on the quercetin standard curve. These findings highlight the potential of gandarusa leaves as a promising source of bioactive compounds for pharmaceutical development.