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Journal : Microbiology Indonesia

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Characterization of Antimicrobial Bacteriocin Produced by Bacillus cereus SS28 Isolates from Budu, a Traditionally Fermented Fish Product of West Sumatera YUSRA YUSRA; FAUZAN AZIMA; NOVELINA NOVELINA; PERIADNADI PERIADNADI
Microbiology Indonesia Vol. 8 No. 1 (2014): March 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.167 KB) | DOI: 10.5454/mi.8.1.4

Abstract

Bacillus cereus SS28 isolated from budu, a fermented fish product from West Sumatra, produced antimicrobial compound that had broad spectrum of inhibition against five microorganisms (Escherichia coli, Staphylococcus aureus, Salmonella thypi, Bacillus subtilis, and Listeria monocytogenes). The aims of this research are characterization of Bacillus cereus SS28 antimicrobial activity and observation of its effect to the cellular morphology of Staphylococcus aureus with electron microscope. Antimicrobial compound produced by Bacillus cereus SS28 was stable at pH range between 2 and 11 and to heating at 121 °C for 15 min. Maximum antimicrobial activity was expressed at pH 2-3 and 70 °C for 45 min. The activity remained after 15 min exposure to UV light. The main changes observed under SEM and TEM were the alteration of Staphylococcus aureus structural cell membrane 48 h after exposure to the antimicrobial compound from Bacillus cereus SS28.
Co-Authors Adhitya Jessica Aidila Fitria, Eddwina Aisman Aisman Alfi Asben Amaliyah Tarumiyo, Aurelia Aninda T. Puari Anisa Kurniati Anjela, Novi Anwar Kasim Arifatulhuda Rifka Athanasia Amanda Septevani Aurelia Amaliyah Tarumiyo Awalina, Rahmi Azzahra, Yasmin Bahar, Rini Betty Sri Laksmi Jenie Daimon Syukri Deden Dermawan Derosya, Vioni Desniorita Desniorita Dian Pramana Putra, Dian Pramana Diana Sylvi Dini Hari, Purnama E Efrina Efrina, Efrina Emriadi - Fansukri, Abdullah Haekal Marzie Faramida, Shella Fauzan Azima Felga Zulfia Rasdiana Firdausni Firdausni Firdausni Firdausni Gunarif Taib Gustiarini Rika Putri Hanifah Andryani Hasbullah Hasbullah Hasbullah Hasbullah Husni, Nayli Ismed Ismed Ismed Jeany Ristia Jessica, Adhitya Kusumaningsih, Indriati Lailita Nurrahmi Putri Linda Wati Maggy T Suhartono Maryam, Maryam Meuthia Fiana, Risa Mislaini Mislaini Neswati Neswati Netty Sri Indeswari Novizar Nazir Novizar Novizar Novizar, Nazir Periadnadi Periadnadi Purnama Dini Hari Purnama Dini, Hari Putri, Lailita Nurrahmi Qurrata A'yun Qurrata A'yun Rahma, S.A. Ratni Kumala Sari Reni Koja Retti Ninsix Rezy Gusmita Rifka, Arifatulhuda Rifma Eliyasmi Rina Yenrina Rini Rini Rini Rini Risa Meuthia Fiana Risa Meutia Fiana Ristia, Jeany Rudi Alfiansyah Sahadi Didi Ismanto Sari, Ratni Kumala Sayuti, Kesuma Siska Ariani Soewarno T Soekarto Surini Siswarjono Susono Saono Tuty Anggraini Wellyalina, Wellyalina Welya Refdi, Cesar Weni Fika Wenny Surya Murtius Wiwit Juita Sari Yasmin Azzahra YUSRA YUSRA Yusra Yusra Zaadah Zaadah Zaadah, Zaadah