Claim Missing Document
Check
Articles

Found 19 Documents
Search

Gambaran Darah Penyu Hijau (Chelonia mydas) Dengan Atau Tanpa Fibropapillomatosis Kadek Intan Dwityanti Devi; Ida Bagus Windia Adnyana; I Gede Soma
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.661 KB) | DOI: 10.19087/jveteriner.2021.22.2.278

Abstract

Penyu merupakan salah satu hewan yang keberadaannya terancam punah. Di Bali temuan kasus penyelundupan penyu sering terjadi, beberapa dari penyu hijau ini mengalami fibropapillomatosis (FP) yang merupakan tumor yang ditemukan pada semua spesies penyu, tetapi paling banyak ditemukan pada penyu hijau. FP ditandai dengan epizootic tumor kulit, flippers, jaringan periokular, karapas, dan plastron. Pemeriksaan hematologi menjadi metode diagnostik, dengan melihat adanya kenaikan atau penurunan jumlah sel darah. Penelitian ini menggunakan sampel penyu hijau dari sitaan Polsek Kuta dengan jumlah penyu hijau dengan fibropappilomatosis 5 ekor dan tanpa fibropapillomatosis 5 ekor. Pengumpulan data pada penelitian ini dilakukan dengan pemeriksaan hematologi. Pemeriksaan hematologi meliputi total eritrosit, nilai hematokrit, dan kadar hemoglobin. Data yang telah diperoleh dianalisis dengan analisis kuantitatif dan dijelaskan secara deskriptif. Hasil gambaran darah penyu hijau dengan fibropapillomatosis dengan rata-rata total eritrosit sebesar 0,55 x 106/µL ± 0,17, PCV 31,4% ± 2,3, Hb 7,2 g/dL ± 1, serta indeks eritrosit, meliputi MCV 616,7 fl ± 176,8, MCH 140,4 pg ± 31,3, MCHC 23,1 g/dL ± 1,9. Gambaran darah dari penyu hijau tanpa fibropapillomatosis memiliki rata-rata total eritrosit 0,46 x 106/µL ± 0.18, PCV 29% ± 0,7, Hb 7,1 g/dL ± 0,7, serta indeks eritrosit meliputi MCV 784,7 fl ± 495,1, MCH 191,9 pg ± 123,8, MCHC 24,4 g/dL ± 1,9. Tidak ada perbedaan yang signifikan terhadap gambaran darah penyu hijau dengan atau tanpa fibropapillomatosis.
Hematological Profiles of Green Sea Turtle (Chelonia mydas) Rehabilitated at Turtle Conservation and Education Center, Serangan Island, Denpasar, Bali Leoni Arifah Agustina; Nyoman Sadra Dharmawan; Ida Bagus Windia Adnyana
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.066 KB)

Abstract

Threat of extinction results in the increasing sea turtle conservation efforts through scientific research in recent years. During rehabilitation process, the non-invasive blood analysis is considered to be highly valuable to determine sea turtle’s health. The objective of this study was to assess the hematological profiles of green sea turtles (Chelonia mydas) under rehabilitation at Turtle Conservation and Education Center (TCEC) Serangan Island, Denpasar, Bali. Three mililiters of peripheral blood from healthy green sea turtles were collected from cervical dorsal sinuses and kept in heparinized vacutainer tube before proceeded with hematological evaluation. PCV was determined by microhematocrit method. Diff count and thrombocyte percentage were determined using Giemsa-stained blood smear under the microscope. RBC and WBC total count were determined using hemocytometer. Hemoglobin (Hb) value was determined using Sahli method. Each hematological values were then analyzed using descriptive analysis. The correlation between body condition index (BCI) and hematological values were analyzed using Spearman’s rank correlation test. The mean values evaluated was 7.28 g/dL in Hb, 31.35% in PCV, 0.67 x 106/?L in total RBC count, 479.37 fl in MCV, 111.38 pg in MCH, 23.42 g/dL in MCHC, 4.01 x 103/?L in total WBC count, 1.20 x 103/?L in lymphocyte, 0.21 x 103/?L in monocyte, 0.10 x 103/?L in eosinophil, 2.48 x 103/?L in heterophil and 0.01 x 103/?L in basophil. No significant correlation was found between the BCI and hematological profiles evaluated.
IDENTIFIKASI JEJARING PENGELOLAAN KONSERVASI PENYU HIJAU (Chelonia mydas) MELALUI PENENTUAN KOMPOSISI GENETIK DAN METAL TAG DI LAUT SULU SULAWESI Ni Kadek Dita Cahyani; I.B. Windia Adnyana; I W. Arthana
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 2 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The research used 51 tissue samples taken from 51 individual Green Turtles in Pulau Panjang, EastKalimantan. The isolation of mtDNA was achieved by adding Proteinase K into the samples prior to the utilization ofthe PCR (Polimerase Chain Reaction) technique. The MEGA 3.1 computer program was employed to read the DNAsequences obtained through the PCR technique.Seven different haplotypes were identified from the analyzed samples. The haplotypes are: A3 (N=7; 13.73%),A4 (N=1; 1.96%), A6 (N=2; 3.92%), C3 (N=5; 9.80%), C5 (N=6; 11.76%), C14 (N=5; 9.80%) dan D2 (N=25;49.02%). Tag findings clearly demonstrated that the Green Turtles, which feed on the seagrass beds of Pulau Panjang,originated in different nesting sites, including Sabah and Trengganu in Malaysia, Sangalaki and Derawan inIndonesia, and Phillipine. The MSA (Mixed Stock Analysis) showed that the Green Turtles population in PulauPanjang was composed of several management units (nesting sites), which were Berau (47%), Sulu Sea (34%) andMicronesia (6%).
STUDI EFEKTIVITAS PENGELOLAAN KOLABORATIF KAWASAN PERAIRAN TAMAN NASIONAL BALI BARAT TERHADAP TUTUPAN KARANG HIDUP DAN SOSIAL EKONOMI MASYARAKAT LOKAL Made Iwan Dewantama; N.K. Mardani; I.B. Windia Adnyana
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 2 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.209 KB)

Abstract

Natural conservation management is a very important task that not only aims to save the environment but alsosupport the people that live in it. All variables of life become part of the management goal which often blurs the phasesof management processes towards an effective one. This study aims to measure the effectiveness of conservationmanagement of West Bali National Park (TNBB) based on measured indicators.The methods of measuring effectiveness is based on several indicators (biophysical, socio-economics andgovernance) using the LIT (Line Intercept Transec) method to monitor coral reefs on 8 diving sites in TNBB. Thesocio-economics and governance indicator utilize questionnaire method and discussions with a sample of localcommunity selected randomly from 4 villages around the TNBB zone.The biophysical indicator demonstrate that the amount of coral reef cover in TNBB is 37,1% which falls underthe MEDIUM category. Meanwhile, the socio-economic indicator illustrates that the community relies very little onmarine resources and have an adequate understanding of conservation efforts and sustainable use of naturalresources. Governance indicator shows that stakeholders involvement is proceeding well with a good level ofsatisfaction regarding collaborative management process.Collaborative management of TNBB waters is quite effective in conserving coral reef ecosystem, which is builtfrom awareness, ownership and stakeholder commitment through a cooperation of various organizations called ForumKomunikasi Masyarakat Peduli Pesisir (FKMPP).
IDENTIFIKASI SEKS RASIO TUKIK PENYU HIJAU (Chelonia mydas) DAN PENYU BELIMBING (Dermochelys coriacea) DI BERBAGAI PANTAI PENELURAN UTAMA DI INDONESIA DWI SUPRAPTI; Ida Bagus WINDIA ADNYANA; I Wayan Arthana
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 5 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sex ratio is highly dependent on incubation temperature. The optimum temperature of 28 ° C - 30 ° C can result in sex ratio 1: 1. The high temperature will be produced predominantly female hatchlings, and vice versa. The incubation temperatures influenced by some environmental factors such as rainfall, air temperature, air humidity, sand temperature, sand humidity, sand type and vegetation. This research carried out at the Sukamade beach - East Java, Sangalaki island - East Kalimantan and Jamursba Medi beach - West Papua. Results showed the unbalanced sex ratio of sea turtle hatchlings in each of study area. The Sukamade beach generated 75% of male hatchlings in nests under vegetation, 100% female hatchlings in the nest on open beaches, and 87.5% male hatchlings in the hatchery. While the sex ratio of hatchlings produced on Sangalaki island are 100% male in nests under vegetation, 72.22% male hatchlings in the nest on open beaches and 94.44% male hatchlings in the hatchery. On the Jamursba Medi beach produced 90.9% male hatchlings from nests on open beaches. Based on correlation analysis, a significant correlation showed between sex ratio and incubation temperature with significance of 0.01. While there is no significance correlation showed between sex ratios by environmental factors, with significance of 0.942 for the air temperature variable, 0.340 for the air humidity, 0292 for amount of the rainfall, 0799 for the sand fraction, and 0.331 for the sand humidity. All of these significance are greater than 0.05.
IDENTIFICATION OF THE STOCK/POPULATION OF GREEN TURTLE (Chelonia mydas) IN THE SUKAMADE (EAST JAVA) NESTING BEACH HIDAYATUN NISA PURWANASARI; IB WINDIA ADNYANA
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 5 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Defining the genetic structure of a particular population of marine turtle is an essential ecological aspect to promote their conservation and enhancement because the resources-protect schemes should be made to the each population unit. Mitochondrial DNA (mtDNA) has been proven effective for detecting population structure in nesting population. We use this method to assess the stock/population of green turtle ( Chelonia mydas) in the Sukamade nesting beach. Three haplotypes, i.e. C3, CS, and the new one that we called Sl were found. Haplotype (hd) and nucleotide diversities (?) were calculated to be 0.538 ± 0.115 and 0.00381, respectively. The closest genetic distance was 0.003 (between C3 and CS), and the longest was 0.011 (between C3 and Sl). Comparison between the genetic distances that found in this research and those defined for the Australasian region by Moritz et al ( 2002) is presented as a phylogenetic tree. Pairwise Fst using molecular distances following the model of Tamura-Nei for nucleotide substitution, as well as two other tests, i.e. pairwise Fst using haplotype frequencies, and the Exact test strongly indicates that the nesting population of Sukamade beach is genetically distinct as compared to the other nesting population within the Australasian region.
Dermatitis Suppurative Mengikuti Infeksi Tungau Demodeks dan Sarcoptes pada Anjing Kampung di Jalanan Kota Denpasar Ida Bagus Oka Winaya; Ida Bagus Windia Adnyana; I Ketut Berata; Ida Ayu Pasti Apsari
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.328

Abstract

Tujuan penelitian ini adalah untuk mengetahui penyebab dermatitis dan kerontokan rambut pada anjing jalanan di Kota Denpasar. Dilakukan pencatatan terhadap identitas 75 ekor anjing yang meliputi umur, ras, tipe rambut dan lokasi lesi pada permukaan tubuh. Sampel biopsi kulit dengan ukuran 6 mm diambil dari lesi kulit yang paling parah dengan tanda klinis hiperemia, gatal, adanya krusta, bersisik/scaling, kebotakan/alopecia. Pengambilan sampel biopsi dilakukan dengan terlebih dulu memberikan injeksi anestetik lokal dan dilanjutkan dengan pemberian spraying antiseptik setelah pengangkatan jaringan biopsi. Sampel jaringan kulit kemudian difiksasi dalam larutan neutral buffered formalin 10%. Setelah 24-48 jam dalam cairan NBF jaringan diembedding dalam blok paraffin dengan metode standar. Sedian dengan ketebalan 5 mikron selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan histopatologi terhadap tujuh ekor anjing penderita dermatitis dan kerontokan rambut umur di bawah satu tahun ditemukan kumpulan tungau demodek di beberapa folikel bulu. Eksudat radang netrofil dan eritrosit juga ditemukan pada permukaan epidermis yang nekrosis. Pada pemeriksaan terhadap 26 ekor anjing penderita dermatitis dan kerontokan bulu umur di atas satu tahun ditemukan 24 ekor anjing murni terinfeksi tungau demodeks dan dua ekor terinfeksi tungau demodeks dan sarcoptes. Ditemukan juga adanya larva tungau demodeks di luar folikel rambut. Larva tungau perifolikel terlihat dikelilingi oleh netrofil pada area dermis nekrosis, sedangkan pada anjing yang terinfeksi oleh dua jenis tungau hanya ditemukan respons radang ringan di sekitar folikel rambut.
Patological Changes in Liver and Gall Bladder Of Bali Cattle Infected by Fasciolosis I Made Kardena; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; Ida Bagus Windia Adnyana; I Made Sukada; Kadek Karang Agustina; Putu Agus Trisna Kusuma Antara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p02

Abstract

Fascioliosis is a parasitic disease that infects ruminants and the disease is widely spread in the world. Fascioliosis caused by Fasciola hepatica and Fasciola gigantica that can cause macroscopic and microscopic lesions in the liver and gall bladder of bali cattle. Samples of bali cattle in Pesanggaran slaughter house that infected with fasciolosis were used in this study. The pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The parasite found in the liver and gall bladder, thickening of bile duct mucous were observed on macroscopic examination. However, in microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy and hyperplasia of the bladder epithelium were occurred.
IDENTIFIKASI MAKROZOOBENTOS SEBAGAI BIOINDIKATOR KUALITAS PERAIRAN DI PANTAI TELUK BENOA, BADUNG Sutrisnawati, Eka Ari; Arthana, I Wayan; Windia Adnyana, Ida Bagus
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 17 No 2 (2023)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/EJES.2023.v17.i02.p05

Abstract

Utilization of coastal waters for aquaculture activities or for other activities provides positive value for human welfare but can also provide the potential for reducing water quality. The decline in environmental quality can be seen from changes in components such as biology, chemistry, and beach physics. Changes in chemical and physical components cause sediment to decrease, which will affect the life of marine biotas, such as the community structure, especially the slow-moving and relatively settled biota on the bottom of the waters (macrozoobenthos). Macrozoobenthos are very appropriate to be used as an indicator of changes in water quality due to their benthic nature which is relatively silent or has low mobility so it is strongly influenced by the environment. The purpose of the study was to determine the structure of the macrozoobenthos community and the condition of seawater quality. There were 25 species of gastropods and 3 other classes. The macrozoobenthos species diversity index on the Benoa Bay Coast showed a moderate level of diversity with the highest value of 2.4576. The uniformity index at each station ranges from 0.0894 – 0.5337. The value of the dominance index ranges between 0.000004-0.7656. Based on the relative abundance, the macrobenthos community is dominated by the Gastropod class. The temperature values ??at each station were relatively the same, ranging from 29.3°C– 30.8°C, and the pH was in accordance with quality standards for macrozoobenthos growth. Some indicators of water quality are still below the quality standard threshold, but the concentration of phosphate (PO4) at all locations is already above the predetermined quality standard of 0.015 mg/L?. Keyword: Macrozoobenthos; Bioindicator; Benoa Bay; Bali