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Pengaruh Variasi Delignifikasi NaOH dan Variasi Hidrolisis H2SO4 Terhadap Produksi Bioetanol dari Limbah Umbi Porang Menggunakan Saccharomyces cerevisiae Marliaty, Toety; Ahmad, Adrianto; Bahruddin, Bahruddin
Journal of Bioprocess, Chemical and Environmental Engineering Science Vol 7 No 1 (2026): Journal of Bioprocess, Chemical, and Environmental Engineering
Publisher : Department of Chemical Engineering, Faculty of Engineering, UNRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jbchees.7.1.54-66

Abstract

Second-generation bioethanol from non-food biomass requires pretreatment and hydrolysis conditions that increase fermentable sugar release while keeping the hydrolysate suitable for fermentation. This study evaluated the effects of NaOH delignification (1–2% v/v) and dilute H₂SO₄ hydrolysis (0.25–0.75% v/v) on sugar formation and ethanol production from porang tuber waste using Saccharomyces cerevisiae. Porang chips (60 mesh) were pretreated at 100°C for 60 min at a 1:10 (w/v) solid-to-liquid ratio, washed to near-neutral pH, and subsequently hydrolyzed at 100°C for 60 min at 1:20 (w/v). Structural carbohydrates were quantified by the Chesson–Datta method, reducing sugars by UV–Vis spectrophotometry, and ethanol by distillation followed by refractometry. NaOH at 1.5% resulted in the most favorable compositional change, increasing cellulose to 55.3% and reducing lignin to 7.0%. The highest initial glucose in the hydrolysate (41.22 g/L) and the best ethanol performance were obtained at NaOH 1.5%–H₂SO₄ 0.5%, reaching 8.62 g/L ethanol, a yield (Yp/s) of 0.434 g/g, and 85.0% of the theoretical yield. Overall, within the tested NaOH–H₂SO₄ ranges, moderate pretreatment and hydrolysis levels provided a practical balance between sugar release and fermentation performance at bench scale. Measurement variability and uncertainty (replicates and dispersion metrics) are reported in the Results to support the observed trends
Sustainable Biohydrogen Production from Palm Oil Mill Effluent: Effect of Hydraulic Retention Time in a Hybrid Anaerobic System Ahmad, Adrianto; Evelyn, Evelyn; Andrio, David; Avriliani, Dini; Dalil, M.; Hamzah, Amir
Leuser Journal of Environmental Studies Vol. 4 No. 1 (2026): April 2026
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/ljes.v4i1.379

Abstract

Currently, the world is facing two crises: a shortage of fossil fuels and global climate change. Climate change is linked to increased environmental damage from fossil fuel use and the effects of greenhouse gases. Therefore, it is important to achieve breakthroughs to develop alternative energy sources that can replace fossil fuels. One of these is biohydrogen, which plays an important role in future energy because it is environmentally friendly, renewable, and sustainable. In addition, Indonesia is the world's largest producer of palm oil, which naturally generates liquid waste. Using palm oil mill liquid waste to produce biohydrogen via an anaerobic hybrid bioreactor during the acidogenesis phase is the best solution to address environmental impacts while simultaneously providing a clean energy source. This research aims to produce biohydrogen from palm oil mill liquid waste. This was done using an anaerobic hybrid bioreactor during the acidogenesis phase, with hydraulic retention times of 6, 12, and 18 hours. The research results show that the best hydraulic retention time is 18 hours, with a VSS removal efficiency of 98% and biogas production of 5.0 L/day, yielding 64% biohydrogen.