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All Journal Folia Medica Indonesiana Journal Of Vocational Health Studies Journal Health & Science : Gorontalo Journal Health and Science Community JURNAL MUTIARA KESEHATAN MASYARAKAT Jurnal Kesehatan Masyarakat dan Lingkungan Hidup Jurnal Ners Jurnal Scientia Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences International Journal Of Science, Technology & Management (IJSTM) Archives of The Medicine and Case Reports Jurnal EduHealth Jurnal Ekonomi INTERNATIONAL JOURNAL OF CULTURAL AND SOCIAL SCIENCE Jurnal Multidisiplin Madani (MUDIMA) JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) International Journal of Health and Pharmaceutical (IJHP) Jurnal An-Najat: Jurnal Ilmu Farmasi dan Kesehatan Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health
Ermi Girsang
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Journal : Jurnal Multidisiplin Madani (MUDIMA)

 1 
Anti-Bacterial Activity Test of Carrot Tuber Ethanol Extract Hydrogel Preparation (Daucus Carota L.) in Inhibiting the Growth of Bacteria and Their Ability in the Healing Process of Grade II Burns on Wistar Rat (Rattus Norvegicus) Welly Ria Utami Lubis; Ali Napiah Nasution; Hanjaya; Alvin Abrar Harahap; Muhammad Nawal Hasya; Jeri Yuliansyah; Ermi Girsang
Jurnal Multidisiplin Madani Vol. 3 No. 6 (2023): June, 2023
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/mudima.v3i6.3529

Abstract

Carrots are root vegetables that are very common in Indonesia. Carrot plant (Daucus carita L.)  is a root vegetable plant that has a high content of beta carotene, rich in dietary fiber, natural antioxidants and high vitamin A which is 12,000 IU. The reddish-yellow color of carrots is due to the high content of carotene pigment. The sample used in this study was fresh carrot tubers. The tubers are separated from other impurities and then washed thoroughly and then drained and weighed. Next, the tubers are dried at a temperature of 30-40oC until the tubers are dry (marked when broken brittle). Simplisia that has dried (brittle) is powdered with a blender and stored in a tightly closed container and stored at room temperature. The preparation of the extract is carried out by maceration using 70% ethanol solvent. According to the Indonesian Herbal Pharmacopoeia (2008), as much as 1 part of simplisia dry powder is put into the maserator, added 10 parts of solvent. Soak for the first 6 hours while stirring occasionally, then let stand for 18 hours. Separate the maserat by filtering. Repeat the extraction process at least twice with the same type and amount of solvent. Furthermore, all the mafiber is collected, then evaporated with a  rotary evaporator at a temperature of ±50oC until a thick extract is obtained. The results showed that ethanol extract of carrot tubers with a simplisia weight of 500 grams produced a yield of 3.78% with an extract weight of 68.9 grams. The results below show that carrot tuber ethanol extract shows phytochemical screening results that contain flavonoids, alkaloids, glycosides, tannins and steroids. The results of the measurement of the diameter of the inhibitory zone above showed that ethanol extract of carrot tubers (Daucus carota L.) can inhibit the growth of  Pseudomonas aeruginosa and Staphylococcus aureus  bacteria at concentrations of 10-400 mg / mL. The measurement results obtained the diameter of the inhibitory zone with a strong inhibitory zone response category at all concentration variations. It was found that high concentrations had activity that did not differ significantly from low concentrations. Carrot tuber ethanol extract has a significant difference (p < 0.05) when compared to the positive and negative groups while the results of the measurement of bacterial killing power above show that carrot tuber ethanol extract (Daucus carota L.) can inhibit the growth of Pseudomonas aeruginosa and Staphylococcus aureus bacteria  at concentrations of 10 – 100 mg / mLl
Test the Effectiveness of Carrot Tuber Ethanol Extract Cream Preparation Formulation in Preventing Increased Melanin in Male Wistar Rats Exposed to UVB Light Wulan Dhari; Ali Napiah Nasution; Djohan; Widyaningsih Oentari; Widya Pasca Amir; Anita Merry Cisca; Ermi Girsang
Jurnal Multidisiplin Madani Vol. 3 No. 6 (2023): June, 2023
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/mudima.v3i6.3535

Abstract

This study was conducted with the aim of proving carrot tuber extract (Daucus carota L.) formulated in cream form can prevent an increase in the amount of melanin in the skin tissue of male wistar rats (Rattus norvegicus) exposed to UVB light and see histopathological changes due to carrot tuber extract given as a prevention of increasing the amount of melanin. This study is experimental with a post-test only control group research design. The test method used was testing the amount of melanin modified using a 13 watt Exoterra UVB 200 lamp. The animals tested were rats (Rattus norvegicus) male wistar strain aged 2-3 months, weighing ± 200 grams. Male rats were randomly divided into 6 groups. Each group consists of 5 male rats. The test area on the back of the back is 2x2 cm2. Group I male rats without cream administration as a negative control group, Group II male rats for Parasol Face Sunscreen Cream as a positive group. Group III is the group with the provision of basic ingredient cream and group IV-VI is the group with the administration of 25% carrot tuber extract cream; 50 %; 75 %. topically for 4 weeks.. The evaluation was performed by observing the amount of melanin at week 4 of histopathology analyzing in the test area. The results will be processed with IBM SPSS 21.0 and analyzed with the steps of Descriptive Analysis, Normality Analysis with data using Shapiro-Wilk test and Homogeneity using Levene's test, Comparative Analysis using one way Anova test, followed by Post-hoc test with LSD test. If the data is abnormal, use non-parametric analysis of the Kruskal-Wallis test followed by the Mann-Whitney test. The results showed that extra ethanol of carrot tubers can prevent the increase in the amount of melanin. This was shown by its ability to reduce melanin and histopathological changes in a group of male rats applied carrot tuber ethanol extract cream within 4 weeks
Co-Authors Akmalul Habib Damanik Ali Nafiah Nasution Alvin Abrar Harahap Analita Khoironissa Anita Merry Cisca Arie - Vonikartika Asri Widiya Sitompul Asyrun Alkhairi Lubis Ayu Hasanah Panjaitan Benedictus Juve Bethania Nadi Ida Febrina Siagian Chiuman, Linda Clinton Antony Depi Yunaspi Dewi Dewi Sri Rezeki DIAN MELANI Diana Agustina Ritonga Dirgantara, Oscar Disti Amanda Putri Djohan Djohan Erisman Hondro Eva Kristina Pelawi Fioni Fioni Firdha Muharraran Ginting, Chrismis Novalinda Gusbakti Gusbakti Hanjaya hardiansyah siregar Ibnu Rushd Irfan Aulia Jeri Yuliansyah Liani Br Ginting M. Ridho Fitra Alamsya Mangatas Silaen Maria Seri Arihta Marlinang I. Silalahi Martin Loodwyck Simanjuntak Maya Sari Mutia Muhammad Agus Muljanto Muhammad Insan Andika Muhammad Nawal Hasya Muna Soraya Putri Nadia Zenni Andari Nasution, Ali Napiah Nasution, Sri Lestari Ramadhani Primanita Novi Andriati Putri Satriani Dachi Rakhmat Akbar Dinur Ramadhan Efendi Rapael Ginting Redo Widhio Mahatvavirya Ria Hartaty S. Shelly Hazlina Hararap Saprilliani . Sindy Zelvia SITI AMINAH Sri Lestari Nasution Sri Lestari Ramadhan Sri Lestari Ramadhani Sri Lestari Ramadhani Nasution Sri Ramadhani Nasution Syaifullah Syaifullah Tan Suyono Tommy Adhi Wijaya Vijay Dhillon Waoziduhu Ndruru Welly Ria Utami Lubis Widya Pasca Amir Widyaningsih Oentari Widyaningsih Oentari Winda Tri Mutia Wulan Dhari Yuliani Yuliani Zsa Zsa Ranty Bianca Saragih