Article Per Year (5 Year)
p-Index From 2021 - 2026
1.503
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Indonesian Journal of Cancer Chemoprevention Biopropal Industri Jurnal Kimia Riset Jurnal Biologi Tropis ANNALES BOGORIENSES Narra J Makara Journal of Science Jurnal Sains Materi Indonesia Annales Bogorienses Berita Biologi
Marissa Angelina
Pusat Penelitian Kimia-LIPI, Kawasan PUSPIPTEK Serpong 15314, Tangerang 15314
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health

Published : 17 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 17 Documents
Search

 1   2 
Activity of Ethanol Extract and Fraction Products Leaves Manilkara kauki as Inhibitors Tyrosinase Enzyme Zakri, Dwika Febriana; Putri, Dwi Hilda; Irdawati, Irdawati; Angelina, Marissa
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8295

Abstract

Currently, cosmetics made from chemical (synthetic) ingredients are in demand by the public because they can whiten the skin by inhibiting the formation of melanin, but the lack of public knowledge about the impact of excessive use has encouraged the need for natural ingredients as tyrosinase inhibitors that are safer than synthetic ingredients. This study aims to test the potential of secondary metabolite compounds in M. kauki leaves as inhibitors of tyrosinase enzyme activity by determining the IC50 value. This assay utilizes L-tyrosine and arbutin as positive control substrates, with UV-Vis spectrophotometric absorption measurements taken at a wavelength of 470 nm. The findings revealed that the buthanol fraction exhibited the highest tyrosinase enzyme inhibition, with an IC50 of 189,72 μg/mL. This was followed by the ethanol extract with an IC50 of 191,97 μg/mL, the hexane fraction at 381.50 μg/mL, and the ethyl acetate extract with an IC50 of 448.986 μg/mL. All samples displayed strong inhibitory activity, outperforming arbutin as a positive control, which had an IC50 value of 831.51 μg/mL.
Cytotoxic Test of Cassia alata L. Leaves Ethanol Extracts, Fractions, and Main Compounds against MCF-7 Cells Khoerunisah, Marya Salfia; Angelina, Marissa; Kasiyati, Kasiyati
Indonesian Journal of Cancer Chemoprevention Vol 13, No 3 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss3pp144-151

Abstract

Cancer is the primary cause of death worldwide. Conventional cancer treatment is known to be less than optimal because of its chemoresistance and toxicity to normal cells. The search for cancer drugs from natural ingredients is still being carried out as an effort to overcome these problems. Cassia alata L. leaf extract is known to have antibacterial and antitumor activities. The main compounds of C. alata L. leaves (emodin, aloe-emodin, and kaempferol) have been reported to have antiproliferative activity. This study aimed to examine the cytotoxic activity of the C. alata L. leaves ethanol extracts, fractions, and main compounds against breast cancer cells (MCF-7). Cytotoxic activity was carried out by the MTT method. IC50 was determined by linear regression analysis describing the relationship between concentration and % cell viability. The results showed that aloe-emodin, emodin, and kaempferol had better cytotoxic activity than the extract and fractions of the C. alata L. leaves with IC50 values respectively 12.7 ppm, 18.1 ppm, and 131.3 ppm.Keywords: Breast cancer, cytotoxic assay, Cassia alata L., ketepeng cina, MCF-7.
Formulation and Characterization of Dewandaru Fruit Extract in Nanocarrier System Septiyanti, Melati; Nurfauziyah; Angelina, Marissa; Triana Dewi, Rizna; Fajriah, Sofa; Meliana, Yenny
Jurnal Sains Materi Indonesia Vol. 25 No. 2 (2024): Jurnal Sains dan Materi Indonesia
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jsmi.2024.3121

Abstract

Dewandaru (Eugenia uniflora L.) is a herbaceous plant that grows in tropical and subtropical regions. The fruit extract can prevent oxidative damage and cholinergic changes. It contributes to antihyperglycemic, antihyperlipidemic, and neuroprotective due to its antioxidants and antidepressant effect. It also possesses antibacterial and anti-inflammatory activity and potentially prevents cardiovascular disease and cancer. In this study, dewandaru fruit extract was formulated in the nanocarrier system (nanoemulsion and nanoencapsulation) to preserve product stability and improve product dispersibility and bioavailability. The nanoemulsion optimum formulation condition was obtained on dewandaru fruit extract concentration 10 wt%, oil/surfactant ratio 0.25, and homogenization speed 20.000 rpm, resulting in particle size of 46.4 ± 0.4 nm and polydispersity index 0.480 ± 0.015. The optimum nanoemulsion formulation was further processed to nanoencapsulation along with milling to produce finer particles. The nanoencapsulation milled for 120 minutes produced encapsulation powder with a size of 5.8 ± 3.340 μm. Accordingly, the nanocarrier technology for dewandaru fruit extract promoted a versatile medicinal preparation both in liquid and solid form. However, the size reduction by milling might disrupt the efficiency of the encapsulation release system. Without the proper coating, as orally administrated, the compound rapidly dissolved before it reached the targeted site.
Evaluation of Curcumin-derived Carbon-dots' Inhibitory Activity as SARS-CoV-2 Antiviral Candidate Using Chemical Crosslinking Taharuddin, Audrey Angelina Putri; Yamahoki, Nicholas; Stephanie, Rebecca; Agustiyanti, Dian Fitria; Wisnuwardhani, Popi Hadi; Angelina, Marissa; Rubiyana, Yana; Ningrum, Ratih Asmana; Wardiana, Andri; Desriani, Desriani; Hariyatun, Hariyatun; Iskandar, Ferry; Permatasari, Fitri Aulia; Giri-Rachman, Ernawati Arifin; Fibriani, Azzania
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.232-239

Abstract

In our previous work, we demonstrated that curcumin-derived carbon dots (Cur-CDs) have potential as antivirals for COVID-19. However, the precise mechanism of action remains unclear. This study investigated the potential of Cur-CDs against SARS-CoV-2 by targeting the dimerization of the C-terminal domain of nucleocapsid protein (N-CTD) using chemical crosslinking. Recombinant SARS-CoV-2 N-CTD was expressed, purified, and subjected to chemical crosslinking. The dimerization inhibition ability of Cur-CDs was assessed with ligand concentrations ranging from 0 to 2,000 μg/mL. Successful inhibition —defined as a noticeable reduction in SARS-CoV-2 N-CTD dimer band intensity on SDS-PAGE—was observed when Cur-CDs were present at 8 to 16 times the protein concentration. We hypothesize that Cur-CDs bind to the dimerization residues, preventing non-covalent interactions between monomers and limiting dimer formation. Our findings suggest that Cur-CDs could be a promising antiviral strategy for SARS-CoV-2, especially targeting the dimerization of the nucleocapsid protein. Additionally, this study also highlights the use of chemical crosslinking as a valuable tool for interaction-based drug screening.
Isolation and Characterization of Compounds from the Leaves of Pterocarpus indicus Willd and Their Antioxidant Activity Hartati, Sri; Angelina, Marissa; Meilawati, Lia; Dewijanti, Indah Dwiatmi
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The flavone glycoside was isolated from ethyl acetate fraction of ethanol extract of leaves Pterocarpus indicus Willd. The isolation was conducted by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by gradient, and purified by sephadex-LH20. The structure was elucidated base on spectroscopy data of NMR (1D and 2D), UV, LC-MS and FT-IR. Antioxidant was evaluated using 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical scavenging. The isolation and identification led a stigmasterol as Compound 1 and a new flavonol-glycoside [(2R)-7-hydroxy-3-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)-2-(3,4,5-trihydroxy phe nyl)chroman-4-one] or ptevon-3-D- glycoside as Compound 2. Antioxidant activity of Compound 2 showed IC50 for 18.53 μmol and blank of quercetin was 7.94 μmol and Vitamin C was 40.25 μmol. These compounds and antioxidant activities are the first time reported from this plant.
Development of an inactivated viral transport medium for diagnostic testing in low-resource countries Rahmani, Silmi; Meitha, Karlia; Septiani, Popi; Priharto, Neil; Kamarisima, Kamarisima; Ningrum, Ratih A.; Angelina, Marissa; Agustiyanti, Dian F.; Wisnuwardhani, Popi H.; Nugroho, Herjuno A.; Tan, Marselina I.
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2068

Abstract

Viral transport medium (VTM) is crucial for retaining clinical specimens, such as the virus or its genetic material from the mucus of respiratory tract of coronavirus disease 2019 (COVID-19) suspected patients. However, the locally produced VTM in Indonesia lacks the ability to inactivate the virus, risking the safety of diagnostic personnel. The aim of this study was to formulate inactive VTM (iVTM) incorporating chaotropic agents like guanidine salt, along with anionic detergents, chelators, buffers, and surfactants, to inactivate the virus while maintaining RNA integrity. Viral RNA stability in iVTM (pH 4 and pH 6) was evaluated for 30 days at 4°C and 25–28°C. In vitro inactivation test was performed on SARS-CoV-2 isolate (variant B1). The stability test revealed that storing the clinical specimens in iVTM at pH 6 maintained severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) detectability by qPCR for up to 30 days at cold and room temperatures. Stability assessments conducted over a 4-month period (at 25–28°C) on iVTM with a pH of 6 revealed clear appearance, consistent pH stability, no alteration in the solution color, and no indications of bacterial or fungal contamination. Results from an in vitro inactivation assay demonstrated that iVTM pH 6 eliminated SARS-CoV-2 infectivity within just five minutes of contact. These findings suggest that iVTM pH 6 offers a safer and cost-effective alternative for handling and transportation of clinical specimens.
UJI TOKSISITAS AKUT DELPHINOL® EKSTRAK MAQUI BERRY (Aristotelia chilensis) PADA TIKUS SPRAGUE-DAWLEY BETINA Sari, Windi Yunita; Sim, Kezia Jeconia; Yuliani, Tri; Dewi, Rizna Triana; Angelina, Marissa; Nursiswanto, Heri; Atifah, Yusni
Berita Biologi Vol 25 No 1 (2026): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2026.14648

Abstract

Delphinol® merupakan ekstrak terstandar dari buah maqui berry (Aristotelia chilensis) yang kaya antosianin. Evaluasi toksikologi digunakan untuk menilai keamanan senyawa aktif yang terkandung di ekstrak tanaman. Tujuan penelitian ini menentukan nilai toksisitas (LD50) dari Delphinol®, yaitu ekstrak terstandar buah maqui berry, guna memastikan keamanannya untuk digunakan. Penelitian dilakukan menggunakan 6 ekor tikus Sprague-Dawley betina, terbagi dua kelompok yaitu perlakuan Delphinol® dan normal. Dosis Delphinol® yang digunakan adalah 5000 mg/kg. Gejala klinis toksisitas diamati selama 24 jam pertama setelah pemberian bahan uji untuk memantau kemungkinan terjadinya kematian. Selanjutnya, dilakukan pengamatan klinis harian selama 14 hari, disertai pemantauan berat badan dua kali dalam seminggu guna mengevaluasi potensi efek toksik yang mungkin timbul. Setelah itu dilakukan pengamatan individu terhadap ada tidaknya gejala keracunan dengan cara tikus dikorbankan untuk ambil darah  dengan analisis hematologi dan ALT serta organ vital seperti  otak, jantung, hati, paru-paru, limfa, ginjal, uterus, ovarium diamati makropatologi dan ditimbang beratnya, serta organ hati dan uterus diamati histopatologinya. Hasil penelitian menunjukkan bahwa pemberian Delphinol® pada dosis 5000 mg/kg berat badan tidak menyebabkan kematian maupun gejala toksisitas akut. Dengan demikian, nilai LD50 Delphinol® lebih dari 5000 mg/kg berat badan sehingga masuk dalam kategori aman dan tergolong praktis tidak toksik.
Co-Authors . Megawati A.A. Ketut Agung Cahyawan W Agustiyanti, Dian F. Agustiyanti, Dian Fitria Anak Agung Istri Sri Wiadnyani Andri Wardiana, Andri Antonius Herry Cahyana Aoki, Chie Atifah, Yusni Azzania Fibriani Beti Ernawati Dewi Desriani Desriani Desti, Hidayati Dewijanti, Indah Dwiatmi Dewijanti, Indah Dwiatmi Dwi Hilda Putri ERNAWATI ARIFIN GIRI-RACHMAN Ferry Iskandar HAK HOTTA Hariyatun, Hariyatun Indah D Dewijanti, Indah D Indah Dewiyanti, Indah Irdawati Irdawati Kamarisima, Kamarisima Kasiyati Kasiati Khoerunisah, Marya Salfia Lia Meilawati Megawati - Meitha, Karlia Meliana, Yenny Mirawati Sudiro Muchammad Irsyad, Muchammad Neil Priharto Ningrum, Ratih A. Nugroho, Herjuno A. Nurfauziyah Nursiswanto, Heri Permatasari, Fitri Aulia Popi Hadi Wisnuwardhani, Popi Hadi Putria, Devi Kasih RAHMANI, SILMI RATIH ASMANA NINGRUM Rizna Triana Dewi S, Banjarnahor S D Santi, Mei Ria Saputra, Ziyan Saputri, Ariyanti Sari, Windi Yunita Septiani, Popi Septiyanti, Melati Sim, Kezia Jeconia Soedarmono, Pratiwi Sofa Fajriah Sofna D.S. Banjarnahor Sofna Dewita Sari Banjarnahor, Sofna Dewita Sari Sri Hartati Stephanie, Rebecca Taharuddin, Audrey Angelina Putri Tan, Marselina I. Tri Yuliani, Tri Triana Dewi, Rizna Wisnuwardhani, Popi H. Yamahoki, Nicholas Yana Rubiyana, Yana Zakri, Dwika Febriana