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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Medical Journal of Indonesia Biopropal Industri Jurnal Kimia Riset Jurnal Biologi Tropis ANNALES BOGORIENSES Narra J Makara Journal of Science
Marissa Angelina
Pusat Penelitian Kimia-LIPI, Kawasan PUSPIPTEK Serpong 15314, Tangerang 15314
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health

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Development of an inactivated viral transport medium for diagnostic testing in low-resource countries Rahmani, Silmi; Meitha, Karlia; Septiani, Popi; Priharto, Neil; Kamarisima, Kamarisima; Ningrum, Ratih A.; Angelina, Marissa; Agustiyanti, Dian F.; Wisnuwardhani, Popi H.; Nugroho, Herjuno A.; Tan, Marselina I.
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2068

Abstract

Viral transport medium (VTM) is crucial for retaining clinical specimens, such as the virus or its genetic material from the mucus of respiratory tract of coronavirus disease 2019 (COVID-19) suspected patients. However, the locally produced VTM in Indonesia lacks the ability to inactivate the virus, risking the safety of diagnostic personnel. The aim of this study was to formulate inactive VTM (iVTM) incorporating chaotropic agents like guanidine salt, along with anionic detergents, chelators, buffers, and surfactants, to inactivate the virus while maintaining RNA integrity. Viral RNA stability in iVTM (pH 4 and pH 6) was evaluated for 30 days at 4°C and 25–28°C. In vitro inactivation test was performed on SARS-CoV-2 isolate (variant B1). The stability test revealed that storing the clinical specimens in iVTM at pH 6 maintained severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) detectability by qPCR for up to 30 days at cold and room temperatures. Stability assessments conducted over a 4-month period (at 25–28°C) on iVTM with a pH of 6 revealed clear appearance, consistent pH stability, no alteration in the solution color, and no indications of bacterial or fungal contamination. Results from an in vitro inactivation assay demonstrated that iVTM pH 6 eliminated SARS-CoV-2 infectivity within just five minutes of contact. These findings suggest that iVTM pH 6 offers a safer and cost-effective alternative for handling and transportation of clinical specimens.
Activity of Ethanol Extract and Fraction Products Leaves Manilkara kauki as Inhibitors Tyrosinase Enzyme Zakri, Dwika Febriana; Putri, Dwi Hilda; Irdawati, Irdawati; Angelina, Marissa
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8295

Abstract

Currently, cosmetics made from chemical (synthetic) ingredients are in demand by the public because they can whiten the skin by inhibiting the formation of melanin, but the lack of public knowledge about the impact of excessive use has encouraged the need for natural ingredients as tyrosinase inhibitors that are safer than synthetic ingredients. This study aims to test the potential of secondary metabolite compounds in M. kauki leaves as inhibitors of tyrosinase enzyme activity by determining the IC50 value. This assay utilizes L-tyrosine and arbutin as positive control substrates, with UV-Vis spectrophotometric absorption measurements taken at a wavelength of 470 nm. The findings revealed that the buthanol fraction exhibited the highest tyrosinase enzyme inhibition, with an IC50 of 189,72 μg/mL. This was followed by the ethanol extract with an IC50 of 191,97 μg/mL, the hexane fraction at 381.50 μg/mL, and the ethyl acetate extract with an IC50 of 448.986 μg/mL. All samples displayed strong inhibitory activity, outperforming arbutin as a positive control, which had an IC50 value of 831.51 μg/mL.
Co-Authors . Megawati A.A. Ketut Agung Cahyawan W Agustiyanti, Dian F. Anak Agung Istri Sri Wiadnyani Antonius Herry Cahyana Aoki, Chie Beti Ernawati Dewi Desti, Hidayati Dewijanti, Indah Dwiatmi Dewijanti, Indah Dwiatmi Dwi Hilda Putri HAK HOTTA Indah D Dewijanti, Indah D Indah Dewiyanti, Indah Irdawati Irdawati Kamarisima, Kamarisima Lia Meilawati Megawati - Meitha, Karlia Mirawati Sudiro Muchammad Irsyad, Muchammad Ningrum, Ratih A. Nugroho, Herjuno A. Priharto, Neil Putria, Devi Kasih RAHMANI, SILMI S, Banjarnahor S D Santi, Mei Ria Saputra, Ziyan Saputri, Ariyanti Septiani, Popi Soedarmono, Pratiwi Sofa Fajriah Sofna D.S. Banjarnahor Sofna Dewita Sari Banjarnahor, Sofna Dewita Sari Sri Hartati Tan, Marselina I. Tri Yuliani, Tri Wisnuwardhani, Popi H. Zakri, Dwika Febriana