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Congenital Malformations in Chicken Embryos After Oxybenzone Exposure Saragih, Hendry T.S.; Empra, Desi Eka Putri; Rahmadian, Desti; Shalihah, Fianicha; Primahesa, Alfian; Oktaviana, Shintia; Allimi, Hayu Swari; Septriani, Nur Indah; Nuriliani, Ardaning
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.589-598

Abstract

Topical use of oxybenzone, commonly found in sunscreens, can be absorbed by the skin, and long-term use may cause endocrine disruption, cancer, and teratogenic effects. However, its potential teratogenic effects on embryonic development have not been well-studied. This study aims to determine the impact of oxybenzone exposure on the early stage of embryonic development. Chicken embryos aged 72 hours (20 Hamburger-Hamilton/HH stage) were exposed to a pure oxybenzone for 24 hours at varying concentrations (0, 1, 5, 10, and 20 ppm), each group consisting of 3 embryos. Embryo preparations were made using the wholemount method. Morphological abnormalities were observed with a stereo microscope, and descriptively morphometric measurements were analyzed using ImageJ software. Statistical analysis used One-way ANOVA and Tukey’s test for normally distributed data, while Kruskal-Wallis H and Mann-Whitney U test for non-normally distributed data. This study found that oxybenzone significantly enlarged the embryo, telencephalon, and eye. Several abnormalities were observed in the embryos exposed to oxybenzone, including incomplete closure of the anterior neuropore, concavity in the anterior and lateral of the mesencephalon, and depressions in the tail bud. This study concludes that oxybenzone acts as a teratogen, causing abnormalities in embryonic development, particularly in the central nervous system.
Review: Aplikasi Histopatologi Untuk Praktik Forensik: Review: Applications of Histopathology for Forensic Practice Faulana, Arum Fahmi; Empra, Desi Eka Putri; Rahmadian, Desti; Shalihah, Fianicha; Nuriliani, Ardaning; Retnoaji, Bambang; Saragih, Hendry Tri Sakti SG; Rohmah, Zuliyati; Septriani, Nur Indah
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.6582

Abstract

Forensics plays an important role in investigating crimes because it can reveal everything related to a murder case, including the time and cause of the murder. Histopathology is the study of damage to body tissues and organs which can be used to help investigate crimes. The aim of this literature review is to study the role of histopathology applications for forensic practice. The method used is searching journals and books using Google Scholar, ScienceDirect, NCBI, and PubMed with the keywords histopathology, forensics, diagnosis, death, microscopy, disease, staining, and autopsy. The histology approach helps the pathologist make a clear determination on the cause of death. The aim of histopathology is to perfect the macroscopic diagnosis and act as an additional examination in forensic case management. One of the histopathology methods in the forensic field is the paraffin method with hematoxylin eosin staining and special stains, immunohistochemistry, and MITS. The parameters observed with this method are measurements of pulmonary fat embolism; determining the age of myocardial infarction; assessment of liver steatosis, fibrosis, and cirrhosis; determination of the age of thrombus or thromboembolism; and diagnosis of shock with various diseases. The prospect of forensic histopathology is to complement molecular diagnostic methods, so that the combination of molecular and histology methods can play a maximum role in the field of forensic pathology. The conclusion of this literature review is that histopathology can be used to confirm the diagnosis in forensic cases and has good future prospects if combined with other methods such as molecular.
Anti-Dandruff Shampoo Formulation from Rambutan Leaf Extract (Nephelium lappaceum L.) as Antifungal Malassezia furfur Azzahra, Hasna; Shalihah, Fianicha; Aeniah, Siti; Rahmawati, Inka Pungky; Ningrum, Paula Tyasmita Andar; Wardani, Salma Fauzia; Muslimah, Intan
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 8, No 1 (2023): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v8i1.72727

Abstract

A shampoo is a product that is used to maintain hair. The most common hair problem is dandruff. A microorganism that causes dandruff is Malassezia furfur. Malassezia furfur is the causative microorganism that infects the skin and scalp into dandruff. This fungus can grow quickly if the head has excess oil glands. Rambutan leaf extract contains secondary metabolites that can inhibit fungal growth. Alkaloids can inhibit the growth of fungi because fungi can grow well at a pH of 3,8 – 5,6. Flavonoids and tannins can cause damage to cell membrane proteins, causing changes in membrane permeability and fungal cell membrane lysis. Saponins can break down fungal cells. A shampoo formulation from Rambutan leaf extract sets it apart from other anti-dandruff shampoos because its herbal composition is safe and increases bioavailability benefits. The method used is laboratory experimental. This research procedure is making rambutan leaf extract using a rotary evaporator, making shampoo by compounding the formulation components, growing test mushrooms on PDA media, making test mushroom suspensions, and conducting the research and data collection. The result showed that shampoo from rambutan leaf extract has an inhibition zone diameter of 0.3 cm to 0.6 cm had a moderate growth inhibition response. The results of the antifungal activity test showed that the rambutan leaf extract shampoo concentration of 10%, 15% and 30% can inhibit the growth of fungi with the best inhibitory concentration at 30% in a strong category.