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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Kedokteran dan Kesehatan Buletin Farmatera Jurnal Ners Science Midwifery Nommensen Journal of Medicine Ibnu Sina: Jurnal Kedokteran dan Kesehatan - Fakultas Kedokteran Universitas Islam Sumatera Utara

Christine Verawaty Sibuea

Universitas HKBP Nommensen

Author-ID : 2358429

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Veterinary

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Kadar TNF-α Enkapsulasi Sel Punca Mesenkimal Sufida, Sufida; Sibuea, Christine Verawaty; Silaen, Rachel Teodora; Kuara, Glenessa; Samosir, Sarah Christina; Ginting, Kharnis Marsha Madora
Jurnal Kedokteran dan Kesehatan Vol 20, No 2 (2024): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.20.2.119-123

Sel punca mesenkimal (MSCs) memiliki kemampuan proliferasi dan kemampuan berdiferensiasi yang tinggi, dan efek parakrin yang mengandung banyak faktor pertumbuhan dan sitokin pro-inflamasi. Tumor necrosis factor-α (TNF-α) merupakan salah satu faktor pertumbuhan yang disekresikan oleh MSCs. TNF-α memiliki peran penting dalam pengaturan sistem imun. Terapi seluler penyakit degeneratif dan infeksi menggunakan MSCs memanfaatkan kemampuan proliferasi, diferensiasi dan efek parakrinnya. Kematian sel sebelum mencapai organ target merupakan keterbatasan dalam terapi seluler. Penelitian menggunakan pendekatan enkapsulasi MSCs dikembangkan untuk mengatasi keterbatasan kurangnya retensi MSCs pada terapi seluler dalam mempertahankan kelangsungan hidup dan efek parakrin MSC. Penelitian ini bertujuan untuk mengetahui efek parakrin MSCs berupa kadar TNF-α pada enkapsulasi MSCs. MSCs dienkapsulasi dengan menggunakan alginat yang dicross-linked dengan CaCl2. Enkapsulasi MSCs dikultur selama 21 hari dan dilakukan analisis kadar TNF-α. Penelitian ini menunjukkan bahwa TNF-α pada MSCs mengalami penurunan hingga hari ke-21. Hal ini menunjukkan bahwa enkapsulasi MSCs mempengaruhi kadar TNF-α dan mempertahankan MSCs dari lingkungan luar.

Deselularisasi Hati sebagai Scaffold Rekonstruksi Organoid Hati Sibuea, Christine Verawaty
Buletin Farmatera Vol 6, No 1 (2021)
Publisher : Universitas Muhammadiyah Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/bf.v6i1.4560

The latest approach in tissue engineering techniques is the use of deselularized native organ as scaffolds for organ reconstruction. Scaffolds made from natural or synthetic matrix have many shortcomings in providing ideal extracellular matrix microstructure for cell proliferation and differentiation. Deselularized native scaffold can maintain the extracellular matrix of native organs, as an ideal microstructure scaffold for cell proliferation and differentiation. This technique answers the challenge of high need for liver transplants due to the limitations of liver donors and the need as liver models for drug testing.for drug testing. Liver is a complex organ, so ideal microstructure is needed in liver organoid reconstruction techniques to produce good synthetic and metabolic functions of the liver

KADAR INTERFERON GAMMA (IFN-γ) MIKROENKAPSULASI MSC-CD34+ DENGAN COATING: STUDI PRELIMINARI TERAPI SELULER MDR-TB Halawa, Karina Mutiarani; Sitanggang, Ervina Julien; Napitupulu, Runggu; Sibuea, Christine Verawaty
Ibnu Sina: Jurnal Kedokteran dan Kesehatan - Fakultas Kedokteran Universitas Islam Sumatera Utara Vol. 23 No. 2 (2024): Juli 2024
Publisher : Faculty of Medicine Universitas Islam Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/ibnusina.v23i2.617

Multidrug Resistant Tuberculosis (MDR-TB) merupakan kondisi di mana pasien tuberkulosis resistan terhadap minimal dua jenis obat anti tuberkulosis. Pasien MDR-TB sering mengalami efek samping sehingga terapi alternatif perlu dipikirkan. Mesenchymal stem cell (MSC) dan sel punca hematopoietik CD34+ dianggap sebagai alternatif potensial. MSC memiliki sifat imunomodulator, sedangkan CD34+ meningkatkan angiogenesis. Interferon gamma (IFN-γ) merupakan sitokin proinflamasi yang berperan dalam regulasi imunitas tubuh. Penggunaan coating dengan lisat konsentrat trombosit dan mikroenkapsulasi dapat meningkatkan efektivitas terapi. Penelitian ini bertujuan mengetahui rerata kadar IFN-γ pada medium kultur mikroenkapsulasi MSC dan CD34+ dengan coating lisat trombosit. Penelitian ini merupakan penelitian deskriptif observasional. Sampel MSC dan CD34+ yang digunakan masing-masing berasal dari tali pusat dan darah asal tali pusat bayi baru lahir. Kadar IFN-γ diukur menggunakan spektrofotometer dengan panjang gelombang 450nm. Hasil penelitian menunjukkan kadar IFN-γ yang disekresikan pada hari ke-2 berjumlah 0,0597 pg/mL, mengalami penurunan pada hari ke-7 dan hari ke-14, kemudian meningkat pada hari ke-21 sebanyak 2,7337 pg/mL.

Viabilitas Hepatosit pada Monokultur 3D Metode Hanging Drop dan Monokultur 2D Sibuea, Christine Verawaty; Hutabarat, Enjelin Sasa Kristanti; Simangunsong, David M. T
Nommensen Journal of Medicine Vol 7 No 2 (2022): Nommensen Journal of Medicine: Edisi Februari
Publisher : Universitas HKBP Nommensen

Background: Liver is a vital organ that has many functions including metabolism, detoxification of toxins and protein synthesis. The prevalence of liver disease is high and the treatment of liver disease continues to develop, so liver models are needed to study liver disease mechanisms and test drug toxicity. Many hepatocyte culture methods are being developed to construct an optimal liver model. The best culture method maintains high hepatocyte viability. Objective : This study aims to determine the viability of hepatocytes in 3D hanging drop method culture and in 2D culture. Methods: Hepatocytes were isolated from the liver of Sprague Dawley-Rats rats (n=2) and digested with Trypsin. Primary hepatocytes were cultured using the hanging drop method and conventional (2D) method. Hepatocyte viability was analyzed using the Trypan Blue Exclusion Test. Results: Hanging drop method had higher viability (81.18%) than the 2D culture method (69.22%) with p>0,05. Conclusion: The hanging drop method has better viability than the 2D culture method. Keywords: Hepatocyte, viability, 3D culture, 2D culture, hanging drop Latar belakang: Hati merupakan organ vital tubuh yang memiliki banyak fungsi diantaranya metabolisme, detoksifikasi racun dan sintesis protein. Prevalensi penyakit hati tinggi dan terapi penyakit hati terus berkembang, sehingga model hati sangat dibutuhkan untuk mempelahari mekanisme penyakit hati dan uji toksisitas obat. Banyak metode kultur hepatosit yang terus dikembangkan untuk menghasilkan suatu model hati yang optimal. Metode kultur yang terbaik adalah metode kultur dengan viabilitas hepatosit yang tinggi. Tujuan : Penelitian ini bertujuan untuk mengetahui viabilitas hepatosit pada kultur 3D metode hanging drop dan pada kultur 2D. Metode: Hepatosit diisolasi dari hati tikus Sprague Dawley-Rats (n=2) dan digesti dengan Tripsin. Hepatosit primer tikus dikultur dengan metode hanging drop (tetes gantung) dan metode konvensional (2D). Viabilitas hepatosit dianalisa dengan menggunakan Trypan Blue Exclusion Test. Hasil: Viabilitas hepatosit pada metode hanging drop memiliki viabilitas lebih tinggi (81,18%) dibandingan dengan metode kultur 2D (69,22%) dengan nilai p<5. Kesimpulan: Metode hanging drop memiliki viabilitas yang lebih baik dibandingkan metode kultur 2D. Kata Kunci: Hepatosit, viabilitas, kultur 3D, kultur 2D, hanging drop

Pengaruh Penggantian Medium terhadap Viabilitas Hepatosit Kultur 3D Organoid Hati Sibuea, Christine Verawaty; Pawitan, Jeanne Adiwinata; Antarianto, Radiana
Nommensen Journal of Medicine Vol 7 No 2 (2022): Nommensen Journal of Medicine: Edisi Februari
Publisher : Universitas HKBP Nommensen

Background : Liver organoids can be used as materials for Bioartificial Liver, to study the mechanism of liver disease and as drug test toxicity. Reconstruction of liver organoids requires optimal culture methods, culture medium and cellular components to construct liver organoids that resemble liver microstructure in vivo with optimal function. 3D culture method using hepatocytes and stem cells with PRP supplemented William's E can reconstruct liver organoids with liver function. Medium exchange is an usual method to maintain the required nutrients and to eliminate waste products, but it requires a sufficient supply of medium and supplementation. Method and the use of effective and efficient medium with optimal hepatocyte viability are urgently needed in the reconstruction of liver organoids. Objective : This study was aimed to compare the viability of primary hepatocytes in culture medium exchange liver organoids and monoculture and without culture medium exchange. Methods : Primary hepatocytes isolated from Sprague Dawley-Rats mice (250gr, n=3) were co-cultured with umbilical cord mesenchymal stem cells, cord blood CD34+ stem cells and LX2 in PRP-supplemented William's E for 14 days. The culture medium was exchanged at 48 hours, day 7 and day 14 and no culture medium exchanged in the control group. Hepatocyte viability was analyzed using the Trypan Blue Exclusion Test at 48 hours, day 7 and day 14. Results : Hepatocyte viability in culture medium exchange liver organoids was higher than without culture medium exchange, especially in monoculture, but there was no significant difference (p value> 0.05). Conclusion: Hepatocyte viability in culture medium exchange liver organoids was not significantly different from no culture medium exchange liver organoids. Culture medium exchange in monoculture supported hepatocyte viability up to day 14. Keywords: hepatocytes, liver organoids, viability, culture medium ABSTRAK Latar belakang : Organoid hati dapat digunakan sebagai bahan Bioartificial Liver, mempelajari mekanisme penyakit hati dan uji toksisitas obat. Rekonstruksi organoid hati membutuhkan metode kultur, medium kultur dan komponen seluler yang optimal untuk menghasilkan organoid hati yang menyerupai mikrostruktur hati in vivo dengan fungsi yang optimal. Metode kultur 3D menggunakan hepatosit dan sel punca mesenkimal dengan William’s E yang disuplementasi PRP dapat merekonstruksi organoid hati dengan fungsi hati. Pergantian medium merupakan metode yang sering dilakukan untuk mempertahankan nutrisi yang dibutuhkan dan untuk membuang sisa metabolit sel, tetapi membutuhkan persediaan medium dan suplementasi yang cukup banyak. Metode dan penggunaan medium yang efektif dan efisien dengan viabilitas hepatosit yang optimal sangat dibutuhkan dalam rekonstruksi organoid hati. Tujuan : Penelitian ini bertujuan untuk mengetahui perbandingan viabilitas hepatosit primer pada organoid hati dengan pergantian medium kultur dan tanpa pergantian medium kultur. Metode : Hepatosit primer yang diisolasi dari tikus Sprague Dawley-Rats (250gr, n=3) diko-kultur dengan sel punca mesenkimal asal tali pusat, sel punca CD34+ asal darah tali pusat dan LX2 dalam William’s E yang disuplementasi PRP selama 14 hari. Medium kultur diganti pada 48 jam, hari ke-7 dan hari ke-14 dan tidak dilakukan pergantian medium pada kelompok kontrol. Viabilitas hepatosit dianalisa dengan menggunakan Trypan Blue Exclusion Test pada 48 jam, hari ke-7 dan hari ke-14. Hasil : Viabilitas hepatosit pada organoid hati dengan pergantian medium kultur tampak lebih banyak dibandingkan tanpa pergantian medium kultur khususnya pada monokultur, tetapi tidak terdapat perbedaan yang signifikan (nilai p>0,05). Kesimpulan : Viabilitas hepatosit pada organoid hati dengan pergantian medium kultur tidak berbeda secara signifikan dengan organoid hati tanpa pergantian medium kultur. Pergantian medium kultur pada monokultur mendukung viabilitas hepatosit hingga hari ke-14. Kata Kunci : Hepatosit, organoid hati, viabilitas, medium kultur

Polimorfisme Gen Vitamin D Receptor (VDR) BsmI pada Multi Drug Resistant Tuberculosis (MDR TB)" Sibuea, Christine Verawaty; Pardosi, Maria Oktaviana; Simbolon, Ira Maylani; Tampubolon, Paulus Adventus
Nommensen Journal of Medicine Vol 9 No 1 (2023): Nommensen Journal of Medicine: Edisi Agustus
Publisher : Universitas HKBP Nommensen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36655/njm.v9i1.1199

ABSTRACT Background : The increasing cases of Tuberculosis (TB) is a concerned health problem to the world, especially Indonesia, which Indonesia is the 2nd most TB cases in the world. The increase in TB cases is accompanied by the increase in Multi Drug Resistant Tuberculosis (MDR TB) cases. Genetic and immunity host factor, inadequate treatment and bacterial mutations affect the infection of MDR TB. Vitamin D has an important role in immune regulatory to eliminate Mycobacterium tuberculosis, as of it can affect the susceptibility to MDR TB. Purpose : The aim of this study was to determine the polymorphism of the Vitamin D Receptor (VDR) BsmI gene in MDR Tuberculosis. Methods : This study was a case control study involving 32 non-MDR TB patients and 32 MDR TB patients. The VDR gene was restricted by the Restriction Fragment Length Polymorphism (RFLP) method using BsmI enzyme and agarose gel visualization was performed. Results : Homozygous variant GG genotype was found higher in non-MDR Tuberculosis (43.75%) than in MDR Tuberculosis (18.8%), and heterozygotes AG genotype was not found in MDR Tuberculosis either in non MDR Tuberculosis. There was no significant relationship between the VDR BsmI gene polymorphism and MDR tuberculosis (p>0.05). Conclusion : This results showed that BsmI VDR gene polymorphism does not affect the susceptibility to MDR Tuberculosis. Keywords : VDR gene, polymorphism, MDR tuberculosis, non-MDR tuberculosis, RFLP ABSTRAK Latar belakang : Peningkatan kasus Tuberkulosis (TB) merupakan masalah kesehatan yang menjadi perhatian bagi dunia khususnya Indonesia, dimana Indonesia merupakan negara dengan kasus TB terbanyak ke-2 di dunia. Kasus TB yang meningkat disertai juga dengan peningkatan kasus Multi Drug Resistant Tuberculosis (MDR TB). Faktor pejamu genetik dan imunitas, pengobatan yang tidak adekuat dan mutasi bakteri, mempengaruhi terjadinya MDR TB. Vitamin D memiliki peran penting dalam regulasi imunitas tubuh untuk mengeliminasi mycobacterium tuberculosis, sehingga dapat mempengaruhi kerentanan seseorang menderita MDR TB . Tujuan : Penelitian ini bertujuan untuk mengetahui polimorfisme gen Vitamin D Receptor (VDR) BsmI pada MDR Tuberkulosis. Metode : Penelitian ini merupakan penelitian case control yang melibatkan 32 penderita non MDR TB dan 32 penderita MDR TB. Gen VDR direstriksi dengan metode Restriction Fragment Length Polymorphism (RFLP) menggunakan BsmI dan dilakukan visualisasi gel agarose. Hasil : Genotif homozigot varian GG lebih banyak ditemukan pada non MDR Tuberkulosis (43,75%) dibandingkan pada MDR Tuberkulosis (18,8%) dan genotif heterozigot AG tidak terdapat pada semua subjek. Tidak terdapat hubungan yang signifikan antara polimorfisme gen VDR BsmI dengan MDR Tuberkulosis (p>0,05). Kesimpulan : Polimorfisme gen VDR BsmI tidak mempengaruhi kerentanan menderita MDR Tuberkulosis. Kata Kunci : Gen VDR, polimorfisme, MDR Tuberkulosis, Non MDR Tuberkulosis, RFLP

Unmethylated Vitamin D Receptor (VDR) pada Multi Drug Resistant Tuberculosis Sibuea, Christine Verawaty; Sinaga, Janry; Zega, Elsa Bea Nibasi; Zebua, Restin Julianti; Batee, Selvian
Nommensen Journal of Medicine Vol 9 No 1 (2023): Nommensen Journal of Medicine: Edisi Agustus
Publisher : Universitas HKBP Nommensen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36655/njm.v9i1.1203

ABSTRACT Background : Cases of Tuberculosis (TB) and multi-drug resistant Tuberculosis (MDR TB) caused by mycobacterium tuberculosis are high globally. Vitamin D has an important role against TB infection by inducing antimicrobial peptides such as cathelicidin through vitamin D receptors (VDR). Several studies suggest that there is a genetic influence for TB infection, such as polymorphism and methylation. Methylation of VDR gene is one of the causes of susceptibility to TB infection. Objective : This study aimed to describe the unmethylated vitamin D receptor gene in patients with MDR TB and pulmonary non-MDR TB in Medan City. Methods : This research was a descriptive studied with a cross sectional research design. Stored DNA sample isolated from blood 40 patient MDR TB blood and 40 patient non MDR TB was converted to bisulfite with EZ DNA Methylation-Gold Kit. Converted DNA was amplified by PCR and finally gel electrophoresis was performed with agarose gel to see the unmethylated. Results : Unmethylated VDR gene was higher in MDR TB (17,5%) than in non MDR TB (null). Unmethylated VDR gene was found higher in men (15%) and in age 18-49 (10%). Conclusion : There was no unmethylated VDR gene in non MDR TB. The unmethylated VDR gene was found in MDR TB. Keywords : MDR TB, VDR gene, unmethylated ABSTRAK Latar belakang : Tuberkulosis (TB) dan multi drug resistant tuberculosis (MDR TB) yang disebabkan oleh mycobacterium tuberculosis sangat tinggi kasusnya secara global. Vitamin D mempunyai peran penting melawan infeksi TB dengan menginduksi peptida antimikroba seperti cathelicidin melalui vitamin D receptor (VDR). Beberapa penelitian menyebutkan adanya pengaruh genetik dalam terinfeksi TB paru yaitu polimorfisme dan metilasi. Metilasi gen VDR menjadi salah satu penyebab kerentanan terinfeksi TB paru. Tujuan : Penelitian ini bertujuan untuk mengetahui gambaran unmethylated gen VDR pada penderita MDR TB dan non MDR TB di Kota Medan. Metode : Penelitian ini merupakan penelitian deskriptif kategori dengan rancangan penelitian cross sectional. Sampel DNA tersimpan yang diisolasi dari darah 40 penderita MDR dan 40 penderita non MDR TB dikonversi menjadi bisulfit dengan EZ DNA Methylation-Gold Kit. DNA yang terkonversi diamplifikasi dengan PCR dilakukan elektroforesis gel dengan gel agarosa untuk melihat metilasi. Hasil : Unmethylated gen VDR lebih banyak pada MDR TB (15%) daripada non MDR TB (0). Unmethylated gen VDR lebih banyak ditemukan pada laki-laki (15%) dan pada usia 18-49 tahun (10%). Kesimpulan : Tidak terdapat unmethylated gen VDR pada non MDR TB. Unmethylated gen VDR ditemukan pada MDR TB. Kata Kunci : MDR TB, gen VDR, unmethylated

Efek Parakrin Sel Punca Mesenkimal pada Kultur In-Vitro Sibuea, Christine Verawaty; Sitanggang, Ervina Julien; Simaremare, Ade Pryta
Nommensen Journal of Medicine Vol 9 No 2 (2024): Nommensen Journal of Medicine: Edisi Februari
Publisher : Universitas HKBP Nommensen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36655/njm.v9i2.1410

Background: MSC, IL-10, TNF-a, IFN-g, PGE2. Background: The high proliferation and differentiation capabilities, paracrine effects and immunomodulatory functions of Mesencymal Stem Cells (MSCs) have been utilized in cellular therapy approaches for infectious and degenerative diseases in the last decade. Paracrine effects MSCs contain growth factors and cytokines, including IL-10, TNF-a, IFN-g and PGE2. There are still debates regarding the role and performance of the levels of paracrine effects of MSCs IL-10, TNF-a, IFN-g and PGE2, so it is necessary to have a model of the performance of these paracrine effects. Objective: To determine the levels of IL-10, TNF-a, IFN-g and PGE2 in basal in-vitro culture conditions, which can be used as a model for MSC immunomodulatory performance. Methods: This research is an in-vitro culture study of MSCs in basal conditions. MSCs were cultured for 21 days and levels of IL-10, TNF-a, IFN-g and PGE2 were examined on the 7th, 14th and 21st days in the culture medium, using the ELISA method. Results: IL-20 was not found in the culture medium until the 21st day, meanwhile TNF-a levels continued to increase until the 21st day. IFN-g was only found on day 7 of culture. PGE2 levels were found to be the highest secreted among the cytokines analyzed, but were not found on day 21. Conclusion: Paracrine effects secreted by MSCs in basal culture conditions due as a response to cell damage that occurs during the duration of culture. The paracrine effects of MSCs can influence each other, so that there can be suppression or induction of secretion of paracrine effects. Keywords: MSC, IL-10, TNF-a, IFN-g, PGE2. Abstrak Latar belakang: Kemampuan proliferasi dan diferensiasi yang tinggi, efek parakrin serta fungsi imunomudulator Mesencymal Stem Cells (MSCs) dimanfaatkan dalam pendekatan terapi seluler penyakit infeksi dan degeneratif dalam dekade terakhir. Efek parakrin MSCs mengandung growth factor dan sitokin, diantaranya IL-10, TNF-a, IFN-g dan PGE2. Banyak perbedaan pendapat tentang peran dan gambaran kadar efek parakrin MSCs IL-10, TNF-a, IFN-g dan PGE2 sehingga diperlukan adanya model gambaran efek parakrin tersebut. Tujuan: Mengetahui kadar IL-10, TNF-a, PGE2 dan IFN-g pada kondisi basal kultur in-vitro, yang dapat digunakan sebagai model gambaran imunomodulator MSC. Metode: Penelitian ini merupakan penelitian kultur in-vitro MSCs pada kondisi basal. MSCs dikultur selama 21 hari dan dilakukan pemeriksaan kadar IL-10, TNF-a, PGE2 dan IFN-g pada hari ke-7, ke-14 dan ke-21 pada medium kultur, dengan menggunakan metode ELISA. Hasil: IL-20 tidak ditemukan pada medium kultur hingga hari ke-21, sedangkan kadar TNF-a semakin meningkat hingga hari ke-21. IFN-g hanya ditemukan pada kultur hari ke-7. Kadar PGE2 ditemukan paling tinggi disekresikan diantara sitokin yang dianalisa, tetapi tidak ditemukan pada hari ke-21. Kesimpulan: Efek parakrin disekresikan MSC pada kultur kondis basal sebagai respon kerusakan sel yang terjadi selama durasi kultur. Efek parakrin MSC dapat saling mempengaruhi, sehingga dapat terjadi supresi atau induksi sekresi efek parakrin. Kata Kunci: MSC, IL-10, TNF-a, IFN-g, PGE2.

Encapsulated Mesenchymal Stem Cell as Regenerative Alternative for MDR-TB: A Gene Expression Analysis of ABCG2 Efflux Sibuea, Christine Verawaty; Marpaung, Dina Octafrida; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Masal, Cindy Destasya; Laia, George Azriel Buala Nama; Tambunan, Octora Angelica Violyn; Samosir, Wanda Lucia; Sidabalok, Cici Kres
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.12040

Multidrug-resistant tuberculosis (MDR-TB) poses a significant global health challenge due to poor treatment outcomes. Encapsulated mesenchymal stem cells (MSCs) have emerged as a potential alternative therapy; however, their role in modulating bacterial drug sensitivity remains unclear. This study aimed to evaluate the expression of the ABCG2 efflux pump gene in encapsulated MSCs co-cultured with MDR-TB, and to explore its implications for bacterial sensitivity to anti-tuberculosis drugs. An in vitro experimental design was employed using encapsulated MSCs cultured with MDR-TB. Total RNA was isolated, converted into complementary DNA (cDNA), and analyzed using quantitative real-time PCR (RT-PCR). Gene expression levels were quantified using the Livak (ΔΔCt) method. Results demonstrated a progressive increase in ABCG2 gene expression on days 2, 7, and 14. Although this increase may reduce the direct antibacterial capacity of MSCs, previous studies have shown that their preserved paracrine function remains beneficial for immunomodulation. These findings support the continued investigation of encapsulated MSCs as an adjunctive therapy for MDR-TB, particularly through immunoregulatory mechanisms despite increased ABCG2 expression.

TNF-α and IL-10 as paracrine effect of encapsulated mesenchymal stem cells coating by platelet lysate Sibuea, Christine Verawaty; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Silaen, Rachel Teodora; Kuara , Glenessa; Samosir, Sarah Christina; Ginting, Kharnis Marsha Madora; Yana, Hiqmah Yusi; Pratama, Gita; Mutiara, Mutiara; Angeline, Wiedya Kristianti
Science Midwifery Vol 11 No 6 (2024): February: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i6.1427

Mesenchymal stem cells (MSCs) have been used as a cellular therapy for infectious and degenerative diseases due to their paracrine effect, immunomodulatory capability, high ability differentiation, and high plasticity. The paracrine effect of MSCs releases many growth factors and pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), enabling them to modulate the immune system. Nevertheless, there are many obstacles to maintaining paracrine effects in cellular therapy due to a shortage of cellular retention. MSC encapsulation provides a favourable environment for the enhanced viability of MSCs. Platelet lysate is comprised of many growth factors that support the paracrine effect of mesenchymal stem cells (MSCs). In this study, MSCs were encapsulated within alginate, crosslinked using calcium chloride (CaCl2), and subsequently coated with platelet lysate. Encapsulated MSCs coated by platelet lysate were cultured for 21 days and analyzed for IL-10 and TNF-α levels. The findings of our study performed that TNF-α in encapsulated mesenchymal stem cells (MSCs) coated with platelet lysate increased until day 21. IL-10 was retained within the capsule and detected very in day 14. This study showed that encapsulated MSCs coated with platelet lysate affected paracrine effect TNF-α of MSC and retained IL-10 inside the capsule

Co-Authors Angeline, Wiedya Kristianti Antarianto, Radiana Batee, Selvian Ginting, Kharnis Marsha Madora Gita Pratama Halawa, Karina Mutiarani Hutabarat, Enjelin Sasa Kristanti Joseph Partogi Sibarani Kuara , Glenessa Kuara, Glenessa Laia, George Azriel Buala Nama Marpaung, Dina Octafrida Masal, Cindy Destasya mutiara mutiara Napitupulu, Runggu Pardosi, Maria Pardosi, Maria Oktaviana Pawitan, Jeanne Adiwinata Samosir, Christina br Samosir, Sarah Christina Samosir, Sarah Christina br Samosir, Wanda Lucia Sidabalok, Cici Kres Silaen, Rachel Teodora Simangunsong, David M. T Simaremare, Ade P Simbolon , Ira Simbolon, Ira Maylani Sinaga, Janry Sitanggang, Ervina Julien Sufida, Sufida Tambunan, Octora Angelica Violyn Tampubolon, Paulus Adventus Yana, Hiqmah Yusi Zebua, Restin Julianti Zega, Elsa Bea Nibasi

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