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All Journal International Journal of Electrical and Computer Engineering Buletin Peternakan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu dan Teknologi Hasil Ternak TELKOMNIKA (Telecommunication Computing Electronics and Control) Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Conference on Innovation and Application of Science and Technology (CIASTECH) Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan Jurnal Nutrisi Ternak Tropis (JNT) Livestock and Animal Research Chalaza Journal of Animal Husbandry
Muhammad Halim Natsir
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Veterinary Other

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Antioxidant Activity of Unripe Sapodilla Fruit Extract (Manilkara zapota L.) through Nrf2 and SOD Expression in Type 1 Diabetic Mice: Antioxidant activity of Manilkara zapota extract in T1DM Muhaimin Rifa'i; Fikriya Novita Sari; Rizky Senna Samoedra; Setyaki Kevin Pratama; Sri Rahayu; Aris Soewondo; Muhammad Halim Natsir
Journal of Tropical Life Science Vol. 13 No. 1 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.01.12

Abstract

This research aims to analyze the effect of unripe sapodilla fruit extract on endogenous antioxidant expression in T1DM BALB/c mice and its free radical scavenging activity. Manilkara zapota extract (MzE) is an aqueous extract of unripe sapodilla fruit and was obtained by maceration and freeze-drying process. This study used 25 male BALB/c mice with 7-weeks-old of age. They were divided randomly into five groups (n=5) before treatment. A single high dose (145 mg/kg BW) of streptozotocin (STZ) was intraperitoneally injected to induce type 1 diabetes mellitus (T1DM). MzE was given orally once each day for 14 days. Liver cells were isolated and immunoassay with anti-superoxide dismutase (SOD) and anti-nuclear factor erythroid 2-related Factor 2 (Nrf2), and then the results were analyzed by flow cytometry. Diphenylpicrylhydrazyl (DPPH) assay was performed to analyze free radical scavenging. Data were analyzed statistically with one-way ANOVA (p<0.05). The result showed that the glucose levels in diabetic mice after MzE administration were significantly lower than in the DM group. MzE treatment increased the expression of Nrf2 and SOD in diabetic mice. MzE could scavenge DPPH with the IC50 value obtained at 48.35 μg/mL, while ascorbic acid as a control could scavenge DPPH with the IC50 value at 22.24 μg/mL. The increase in the scavenging activity is in line with the increase in extract concentration. In conclusion, this study revealed that MzE can be an endogenous antioxidant enhancer by improving the expression of Nrf-2, SOD and can inhibit free radicals as an exogenous antioxidant in T1DM.
Co-Authors A. Furqon Addharul Muttaqin Adharul Mutaqin Adharul Mutaqin Adli, Danung Nur Ahmad Nur Musyarrof Al Akrom Ajeng Kusumaningtyaswati Alfan Kurniawan Antonius Suwanto Aris Soewondo Asri Nurul Huda Christian Christian Cindy Audina Damayanti Daniel Soares Dhika Yonika Primacitra Dina Marliana Dyah Lestari Yulianti Eko Widodo Esti Puspitasari Fikriya Novita Sari Filoza Marwi Filoza Marwi Gusti Ahmad Fanshuri Alfarisy Haryaka Tisti Sadara Ilmi Sholichatunnisa Ilmiatus Sholichatunnisa Imam Cholissodin Irfan Djunaidi Irfan Djunaidi Irfan H. Djunaidi Iswati Iswati Junaedi Junaedi Khaerunnisa Firdaus Sadikin KUSWATI laudita Setia Busta Ludovika Jessica Virginia Mauldy Putra Pratama Mohammad Farid Muhaimin Rifa'i Muhammad Andika Yudha Harahap Muhammad Irvan Ali Muharlien Muharlien Muharlien Muharlien Nanang Febrianto Natasha Lenzky Mutia Nurul Isnaini O. Sjofjan Obbi Firmansyah Osfar Sjofjan Ratih Eka Dewayani Reo Radius Falah Reynaldy Hadi Ardyansyah Rizki Prafitri Rizky Senna Samoedra S. Chuzaemi S. P. Lestari Setyaki Kevin Pratama Siti Nur Ulpah Sri Rahayu Suyadi Suyadi Syahputra Wibowo Tasya Nur Karina Tika Septi Wardani Tri Eko Susilorini Vitra Vitra Wayan Firdaus Mahmudy Y. F. Nuningtyas Yuli Frita Nuningtyas Yuli Indriani