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All Journal Vegetalika Planta Tropika Bioeksperimen: Jurnal Penelitian Biologi Jurnal Pengabdian UntukMu NegeRI Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Abdi Insani Sehat Rakyat: Jurnal Kesehatan Masyarakat VIGOR: JURNAL ILMU PERTANIAN TROPIKA DAN SUBTROPIKA International Journal of Health Science and Technology JHeS (Journal of Health Studies) Hasil Karya Aisyiyah Untuk Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Wiwit Probowati
Universitas Aisyiyah Yogyakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Identifikasi Bakteri Staphylococcus Aureus pada Spons Make-Up di Kalangan Mahasiswa Putri Maharani; Wiwit Probowati; Widaninggar Rahma Putri
Sehat Rakyat: Jurnal Kesehatan Masyarakat Vol. 5 No. 3 (2026): Agustus 2026
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/sehatrakyat.v5i3.7918

Abstract

Makeup sponges are porous cosmetic application tools that are reused and can potentially become a breeding ground for microorganisms if not cleaned regularly. One bacterium that can contaminate makeup sponges is Staphylococcus aureus, which are knew to do play with a role in various skin also soft tissue infections. This reseach was purposelly to identifying the presence of S. aureus towards makeup sponges used by college students. The study employed a quantitative experimental method using purposive sampling on 30 samples of makeup sponges that had been used for at least 1–2 months. Samples were grouped based on washing frequency: frequently washed, rarely washed, and never washed. Bacterial identification was performed via inoculation on Mannitol Salt Agar (MSA) medium, followed by subculturing, Gram staining, catalase testing, and coagulase testing. The results of the study showed colony growth on MSA medium, indicated by a yellow discoloration of the medium. Gram staining revealed coccus-shaped cells arranged in clusters and stained purple, indicating the characteristics of Gram-positive bacteria. The catalase and coagulase tests yielded positive results, confirming the presence of Staphylococcus aureus. Of the 30 samples examined, 7 positive samples were found in the group of sponges that were rarely washed, 6 positive samples in the group that were never washed, and 3 positive samples in the group that were frequently washed. These findings indicate that S. aureus contamination is more common in sponges that are rarely or never washed compared to those cleaned regularly.
Analisis Tingkat Kontaminasi Bakteri Escherichia Coli pada Air Sumur Gali pada Tahap Sebelum dan Sesudah Perebusan Duwi Yuliana Arini; Wiwit Probowati; Widaninggar Rahma Putri
Sehat Rakyat: Jurnal Kesehatan Masyarakat Vol. 5 No. 3 (2026): Agustus 2026
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/sehatrakyat.v5i3.7919

Abstract

Water was from a native natural fountain that is became vital for human life, particularly as source of drinking water also for things household needs. However, the use of dug well water is highly susceptible to bacteriological contamination. Contamination of drinking water with Escherichia coli is a leading cause of gastrointestinal infections and diarrhea. This reseach was purposelly to identifying the  exsist of E. coli in dug well water also established the impact as well range from septic tank and the effectiveness of the boiling process. The method was using a quantitative approach using an experimental design. The samples including of 14 dug wells gap less than 10 meters also more than 10 meters from a septic tank, with treatments before and after boiling. Laboratory analysis were including use the MPN (Most Probable Number) method thru a presumptive test (Lactose Broth), a confirmatory test (Brilliant Green Lactose Bile Broth), and an identification test (Eosin Methylene Blue Agar). Data were statistically analyzing use technique of Shapiro-Wilk normality test also the non-parametric Wilcoxon signed-rank test. The yields demonstrated that physical distance was not significantly correlated with bacterial contamination levels. The Wilcoxon test revealed a significant and notable difference in bacterial contamination levels before and after boiling, indicating that the boiling process effectively reduced biological contamination in the water. After boiling, 7 samples met drinking water standards (0 MPN/100 ml), while the other 7 samples were still found to contain bacteria due to issues with equipment sterilization or procedure.
Co-Authors Alifiani Hikmah Putranti Andini Bilindade Andini, Nisa Aulia Annaziha, Salma Annisa Khumaira Arif Bimantara Aulia Ulfah Mu’awanah Ayuni, Tiara Sekar Baihaqi, Muhammad Azizan Azmani BUDI SETIADI DARYONO Duwi Yuliana Arini Firyalunfah, Pilar Rosatria Fuad Gandhi Torizal Gailea, Manda Aminah Ika Afifah Nugraheni Isnaini, Febriana Nur Kharissa, Elvia Nur Maharani, Nova Santika Nabila, Tsania Taskia Nisa, Neny Fahrun Nosa Septiana Anindhita Nosa Septiana Anindita Pilar Rosatria Firyalunfah Pramesti, Indah Putri Maharani Rais Sulistyo Widiyatmoko Rika, Rika Silvi Lailatul Mahfida Sunartiyah Sunartiyah Suryadi Suryadi Titin Aryani Tsania Taskia Nabila Wahyuni Wulansari Widaninggar Rahma Putri Widaninggar Rahma Putri Widiyatmoko, Rais Sulistyo Wijayanti, Suprihatin Zahra, Adiba Kanza Az