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An In Vitro Study of The Spore Densities Effect of Trichoderma spp. as Biocontrol Agent for Fusarium Wilt in Cayenne Pepper (Capsicum sp.) Tsania Taskia Nabila; Ika Afifah Nugraheni; Rais Sulistyo Widiyatmoko; Wiwit Probowati
International Journal of Health Science and Technology Vol 3, No 1 (2021): July
Publisher : Universitas 'Aisyiyah Yogyakarta

Root disease controlling has been a challenge in increasing plant productivity. The soil-borne pathogens become the main concern which mostly infects the root surface. One of the most common soil-borne pathogens is Fusarium oxysporum (Fo). Cayenne pepper (Capsicum sp.) is one of the most abundant commodities and contributes significantly to the economy. This plant is also susceptible to Fusarium wilt infection. The use of endophytic fungi as biocontrol agents is an alternative against soil-borne pathogens, one of which is Trichoderma spp. fungi. This study aims to determine the effectiveness of various spore densities of Trichoderma spp. against the pathogen Fo. The research was conducted through an in vitro study by antagonistic testing between spore suspensions of Trichoderma spp. (103, 105, and 107 spores/mL) with Fo. The results showed the density of 107 spores/mL gave the highest percentage of inhibition (54.59%) compared to spore density of 103 (35.27%) and 105 spores/mL (44.44%). The spore density of 107 spores/mL gave a significant difference in response to the density of other spores according to the BNT test at a significance level of 5% and was able to inhibit the growth of Fo more than 50%. The results of this study are expected to be a reference for the formulation of Trichoderma spp. which is ideal for controlling Fo pathogens in the fields.

Uji kandungan logam berat Timbal (Pb) dan Tembaga (Cu) pada kue lumpur menggunakan spektrofotometer serapan atom Wiwit Probowati
JHeS (Journal of Health Studies) Vol 3, No 2: September 2019
Publisher : Universitas 'Aisyiyah Yogyakarta

Tujuan penelitian ini adalah mengetahui kandungan Timbal (Pb) dan Tembaga (Cu) pada kue lumpur yang dijajakan ditepi jalan Pasar X. Metode yang dilakukan dalam penelitian ini dengan destruksi kue lumpur dengan HNO3 pekat dan asam perklorat 70%. Kandungan Timbal dan Tembaga dianalisis menggunakan Spektofotometer Serapan Atom (SSA). Hasil penelitian ini menunjukkan kandungan logam berat Timbal (Pb) dan Tembaga (Cu) kue lumpur yang dijual di tepi jalan Pasar X di luar batas aman untuk dikonsumsi dari Badan Standarisasi Nasional dan Badan Pengawasan Obat dan Makanan RI. Terukur 4.4 ppm untuk kadar Timbal (Pb) dan 1,1 ppm untuk kadar Tembaga (Cu).

Aplikasi Eco-enzym untuk Pemurnian Air Terkontaminasi dan Budidaya Tanaman Obat Keluarga: Application of Eco-enzymes for Purification of Contaminated Water and Cultivation of Family Medicinal Plants Probowati, Wiwit; Wijayanti, Suprihatin; Rika, Rika; Pramesti, Indah; Isnaini, Febriana Nur; Nisa, Neny Fahrun
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 4 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i4.8758

Increased water pollution due to industrial, household, and agricultural waste has triggered the need for environmentally friendly technologies for water purification. Eco-enzyme, a solution fermented from various kinds of fruit and vegetable kitchen waste with the addition of sugar and water is recognized to have anti-bacterial and anti-fungal properties effective in decomposing toxic substances and improving water quality. Utilization of waste enzymes is emerging as a viable strategy to treat contaminated water as well as organic fertilizer in the cultivation of family medicinal plants. This training activity on eco-enzyme making and pouring was carried out in the framework of community service activities and the 2024 eco-enzyme festival. Training on making eco-enzymes was carried out using socialization and demonstration methods. Then the finished eco-enzymes were poured into the river behind the campus of 'Aisyiyah University Yogyakarta and watered in the cultivation of Family Medicinal Plants in Sidoagung village, Godean sub-district, Sleman. Based on the evaluation of the activity, participants were very enthusiastic about understanding new knowledge and were able to practice the results of making eco-enzymes. In addition to creating a clean environment, pouring eco-enzymes into the river reduces the contamination of pollutants. In addition, the application of eco-enzymes to the cultivation of family medicinal plants can function as a liquid organic fertilizer that fertilizes the soil and optimizes plant growth.

fast track: Uji Efektivitas Antibakteri Ekstrak Pare Belut (Trichosanthes cucumerina) Terhadap Bakteri Staphylococcus aureus Dan Escherichia coli Penyebab Infeksi Ulkus Diabetikum Batubara, Zalhan Abiyyu; Astuti, Tri Dyah; Probowati, Wiwit
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 16, No 3 (2025): Juli-September 2025
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar

Ulkus diabetikum merupakan komplikasi kronis diabetes melitus yang rentan mengalami infeksi oleh bakteri seperti Staphylococcus aureus dan Escherichia coli . Resistensi terhadap antibiotik mendorong pemanfaatan alternatif antibakteri dari bahan alam, salah satunya adalah tanaman pare belut ( Trichosanthes cucumerina ). Buah tanaman ini diketahui mengandung senyawa aktif seperti flavonoid, alkaloid, tanin, dan saponin yang memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui efektivitas antibakteri ekstrak buah pare belut terhadap S. aureus dan E. coli penyebab infeksi ulkus diabetikum. Desain penelitian ini adalah eksperimen in-vitro dengan pendekatan post-test only, menggunakan metode Kirby-Bauer. Ekstrak diuji pada konsentrasi 12,5%, 25%, dan 50%, disertai kontrol positif (Gentamisin dan Siprofloksasin) serta kontrol negatif (Aquades). Zona hambat digunakan sebagai indikator aktivitas antibakteri. Hasil menunjukkan bahwa ekstrak buah pare belut lebih efektif terhadap S. aureus (kategori intermediet) dibandingkan E. coli (kategori resisten), dengan peningkatan daya hambat seiring peningkatan konsentrasi. Temuan ini menunjukkan potensi ekstrak sebagai antibakteri alami, meskipun efektivitasnya belum menyamai standar antibiotik. Perbandingan dengan bagian lain dari tanaman pare belut seperti biji, daun, dan batang menunjukkan zona hambat yang lebih besar. Penelitian lanjutan disarankan dengan konsentrasi di atas 50% atau kombinasi antarbagian tanaman untuk meningkatkan efektivitas antibakteri.

PEMANFAATAN UMBI-UMBIAN MINOR PADA MENU JAJANAN ANAK-ANAK DALAM UPAYA PELESTARIAN SUMBER DAYA GENETIK TANAMAN Probowati, Wiwit; Maharani, Nova Santika; Zahra, Adiba Kanza Az; Mahfida, Silvi Lailatul; Gailea, Manda Aminah; Andini, Nisa Aulia
Jurnal Abdi Insani Vol 12 No 8 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i8.2804

Indonesia merupakan negara dengan biodiversitas tinggi seperti halnya tanaman umbi-umbian. Pemanfaatan umbi-umbian minor sebagai sumber bahan pangan semakin menurun. Masyarakat mengenal umbi-umbian minor sebagai makanan kuno yang hanya diolah dengan direbus. Seiring dengan perkembangan zaman yang semakin modern pemanfaatan umbi-umbian minor terlupakan tergantikan oleh makanan olahan modern yang berbahan dasar tepung gandum yang bukan merupakan produk asli Indonesia. Tujuan dari program pengabdian masyarakat yang dilaksanakan di Sleman Yogyakarta ini bertujuan untuk memberikan edukasi dan pelatihan kepada masyarakat akan pentingnya menjaga kelestarian tanaman umbi minor dan pelatihan berbagai olahan pangan dari umbi minor. Metode yang digunakan dalam program ini adalah pembelajaran dan praktik partisipatif. Peserta yang beraal dari Pimpinan Cabang ‘Aisyiyah (PCA) Seyegan berjumlah 50 orang diberikan pembelajaran sambil melakukan praktek. Didapatkan hasil eksplorasi umbi-umbian minor di wilayah Kabupaten Sleman yaitu talas, umbi garut, dan umbi ganyong. Formulasi resep untuk jajanan anak didapatkan bahwa talas dapat dibuat menu kroket, umbi garut dapat dibuat menjadi mie sehat dan umbi ganyong dapat dibuat kukis dan minuman jelly atau dawet. Hasil uji pengetahuan dari 50 peserta program menunjukkan 85% mengolah umbi dengan direbus atau dikukus, 9% digoreng dan 6% peserta menjawab dijadikan minuman. Kegiatan ini sangat bermanfaat untuk menambah pengetahuan tentang umbi-umbian minor dan melatih peserta untuk mengolah menjadi berbagai menu jajanan yang diminati anak-anak. Selanjutnya peserta akan tertarik untuk melestarikan tanaman ini dan mencoba berbagai olahan pangan yang lebih bervariasi.

Perbandingan Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirih dengan Serai Wangi sebagai Desinfektan Alami terhadap Pertumbuhan Bakteri Staphylococcus Aureus Anandarianto, Annisa Shafa; Novalina, Dhiah; Probowati, Wiwit
Malahayati Nursing Journal Vol 7, No 9 (2025): Volume 7 Nomor 9 (2025)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v7i9.21955

ABSTRACT The use of chemical-based disinfectants such as sodium hypochlorite (NaOCl), although effective, poses health and environmental risks. This study aimed to evaluate the antibacterial activity of ethanol extracts of betel leaf (Piper betle L.) and citronella leaf (Cymbopogon nardus) as natural disinfectant alternatives against Staphylococcus aureus. A laboratory experimental design with a post-test only control group was employed. The antibacterial test was conducted using the disc diffusion method with 90% ethanol extracts. Results showed that the bioactive ethanol-derived extract of Piper betle leaves exhibited a quantifiable microbial growth suppression zone with a linear spatial extent of 12 mm, while citronella leaf extract produced 11 mm. The positive control (NaOCl) showed inhibition zones of 32–40 mm, whereas the negative control (distilled water) showed no inhibition. Mann-Whitney statistical test revealed a significant difference between treatments (p<0.05). Betel leaf extract demonstrated greater antibacterial effectiveness compared to citronella. However, both extracts showed lower efficacy than the positive control. A key limitation was the use of distilled water as a diluent, which may have reduced the solubility of non-polar active compounds. Future research is recommended to use more compatible diluents (e.g., low-grade ethanol or DMSO) and assess quantitative indices comprising the Minimum Inhibitory Concentration (MIC), which defines the sub-inhibitory limit for microbial growth suppression, and the Minimum Bactericidal Concentration (MBC) for more detailed quantitative analysis. Keywords: Disinfectant, Betel Leaf, Lemongrass Leaf, Antibacterial Activity Test. ABSTRAK Penggunaan disinfektan berbahan kimia seperti natrium hipoklorit (NaOCl) meskipun efektif, memiliki efek samping terhadap kesehatan dan lingkungan. Fokus investigasi ilmiah ini diarahkan pada pengujian tingkat efikasi bioaktivitas antibakteri yang dimanifestasikan oleh fraksi ekstraktif dari folium Piper betle L. melalui proses pelarutan menggunakan etanol sebagai pelarut polar dan daun serai wangi (Cymbopogon nardus) sebagai alternatif disinfektan alami terhadap Staphylococcus aureus. Skema metodologis yang diimplementasikan terdiri atas simulasi eksperimental berbasis laboratorium, dengan pengaturan desain post-intervensional yang mengadopsi kerangka control group tanpa prosedur pra-intervensi pengukuran. Uji dilakukan terhadap ekstrak etanol konsentrasi 90% dengan mengaplikasikan protokol uji difusi berbasis cakram sebagai metode analitis, observasi eksperimental merefleksikan terbentuknya area inhibisi oleh senyawa bioaktif hasil ekstraksi etanol daun Piper betle dengan ukuran linear ekuivalen sebesar 12 mm, sedangkan ekstrak serai wangi 11 mm. Kontrol positif (NaOCl) menunjukkan zona hambat 32–40 mm, sementara kontrol negatif (aquades) tidak menunjukkan zona hambat. Uji Mann-Whitney menunjukkan perbedaan signifikan antar perlakuan (p<0,05). Ekstrak daun sirih terbukti lebih efektif dibandingkan serai wangi dalam menghambat pertumbuhan S. aureus. Namun, efektivitas kedua ekstrak masih di bawah kontrol positif. Keterbatasan utama penelitian ini adalah penggunaan aquades sebagai pelarut pengencer yang dapat menurunkan efektivitas senyawa non-polar. Penelitian lanjutan disarankan menggunakan pelarut yang lebih sesuai dan melakukan uji MIC serta MBC untuk data kuantitatif. Kata Kunci: Desinfektan, Daun Sirih, Daun Serai Wangi, Uji Aktivitas Antibakteri

OPTIMIZING AMOUNT AND IDENTIFICATION OF YEAST IN SALAK YEAST WATER (Salacca edulis Reinw cv Pondoh) Khumaira, Annisa; Annaziha, Salma; Baihaqi, Muhammad Azizan Azmani; Anindita, Nosa Septiana; Bimantara, Arif; Probowati, Wiwit
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3809

Salak fruit is a tropical fruit spread across Southeast Asia and can potentially be a source of natural yeast by processing fruit yeast water. Salak fruit is known to be rich in carbohydrates, making it a potential natural habitat for microbial communities, especially yeast. This research aims to optimize the growth of yeast in the salak yeast water system by varying the sugar concentration treatment, the composition of the amount of fruit flesh, and the fermentation time, as well as identifying the yeast in the salak yeast water. The method used is to count the number of yeast colonies using the Total Plate Count method. The most optimal treatment results are then tested for pH, total sugar, alcohol, isolation and yeast identification. The research results showed that the composition of salak meat was 30%, adding 1% sugar, and fermentation for 6 days resulted in the most optimal growth of the microbial community with an amount of 3.1×10⁶ cfu/ml. The pH test showed a result of 3.01, the alcohol content, namely ethanol, was 0.066855%, no methanol was detected, the total sugar test result in the yeast water was 2.08%, and it was identified in the yeast water that there were Hanseniaspora opuntiae and Candida sorboxylosa. The results show that salak yeast water (Salacca edulis Reinw cv Pondoh) can be used as a yeast water product to ferment food.

Efektivitas Pupuk Cair Pseudomonas fluorescens Agensia Pengendali Hayati Terhadap Penyakit Mosaik Tanaman Kakao Probowati, Wiwit; Nugraheni, Ika Afifah; Aryani, Titin
Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 1: March 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v7i1.10245

Cocoa is a strategic commodity for plantation in Indonesia. However, pest and disease infections are inevitably common constrains for the cocoa cultivators. Cocoa mosaic disease is one of disease which is cause cocoa trees suffering. Pseudomonas fluorescens are obligate aerobic bacteria that are very valuable for agricultural technology. The bacteria also protects plants from pathogens infection by producing secondary metabolites that kill bacteria and other fungi. The bacteria can used as safe and environmentally friendly biological control agents. This study aims to determine P. fluorescens liquid fertilizer formulation and its effectiveness in suppressing mosaic disease in cocoa plants. The research method was carried out by making a liquid formula from P. fluorescens and then applying it to cocoa plants that were attacked by mosaic disease. Bacterian isolates grown in conch broth medium were incubated for 3 days. Then the fertilizer formula was applied to mosaic symptomatic cocoa plants with fertilizer concentrations of 25%, 50% and 75% compared to spraying using chemical insecticides. The results showed that the liquid formula concentration of 75% can suppress the most effective mosaic symptoms and the optimal growth of cocoa plants for 7 treatments.

OPTIMIZING AMOUNT AND IDENTIFICATION OF YEAST IN SALAK YEAST WATER (Salacca edulis Reinw cv Pondoh) Khumaira, Annisa; Annaziha, Salma; Baihaqi, Muhammad Azizan Azmani; Anindita, Nosa Septiana; Bimantara, Arif; Probowati, Wiwit
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3809

Salak fruit is a tropical fruit spread across Southeast Asia and can potentially be a source of natural yeast by processing fruit yeast water. Salak fruit is known to be rich in carbohydrates, making it a potential natural habitat for microbial communities, especially yeast. This research aims to optimize the growth of yeast in the salak yeast water system by varying the sugar concentration treatment, the composition of the amount of fruit flesh, and the fermentation time, as well as identifying the yeast in the salak yeast water. The method used is to count the number of yeast colonies using the Total Plate Count method. The most optimal treatment results are then tested for pH, total sugar, alcohol, isolation and yeast identification. The research results showed that the composition of salak meat was 30%, adding 1% sugar, and fermentation for 6 days resulted in the most optimal growth of the microbial community with an amount of 3.1×10⁶ cfu/ml. The pH test showed a result of 3.01, the alcohol content, namely ethanol, was 0.066855%, no methanol was detected, the total sugar test result in the yeast water was 2.08%, and it was identified in the yeast water that there were Hanseniaspora opuntiae and Candida sorboxylosa. The results show that salak yeast water (Salacca edulis Reinw cv Pondoh) can be used as a yeast water product to ferment food.

Deteksi Turnip Mosaic Virus (TuMV) pada Tanaman Brokoli di Boyolali, Indonesia: Deteksi Turnip Mosaic Virus (TuMV) pada Tanaman Brokoli di Boyolali, Indonesia Probowati, Wiwit; Firyalunfah, Pilar Rosatria; Nabila, Tsania Taskia
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 9 No 2 (2025): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/e80fdb13

Turnip mosaic virus (TuMV) is one of the emerging viruses that causes serious yield losses of brassica vegetables, including Indonesia. Broccoli (Brassica oleracea var. italica) is a potential horticultural commodity in Indonesia because of its many benefits. The objective of this study was to detect TuMV-infecting broccoli using reverse transcription polymerase chain reaction (RT-PCR) technique. Field survey has been conducted to determine disease symptoms in broccoli field Boyolali, Central Java. This study was conducted through several phases, which are: leaves sample collection on the field, virus RNA isolation, RT-PCR, and TuMV detection using coat protein (CP) specific primer. The result of field observed broccoli plant with several symptom of TuMV infection, such as: mosaic symptom leaf, blister leaf, vein banding, vein clearing, and yellowing of leaf spot. The detection of TuMV by RT-PCR showed that broccoli with those symptoms observed is positively infected by TuMV. Specific DNA band was amplified from infected plant on 800 bp. This study is report of naturally infection of TuMV on broccoli with those symptoms at Boyolali, Central Java, Indonesia.

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