Claim Missing Document
Check
Articles

Found 16 Documents
Search

Kadar Isoflavon Aglikon pada Ekstrak Susu Kedelai dan Tempe Muammar Fawwaz; Ayu Natalisnawati; Muzakkir Baits
Industria: Jurnal Teknologi dan Manajemen Agroindustri Vol 6, No 3 (2017)
Publisher : Department of Agro-industrial Technology, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (733.474 KB) | DOI: 10.21776/ub.industria.2017.006.03.6

Abstract

AbstrakSusu kedelai dan tempe adalah pangan olahan yang digemari masyarakat Indonesia dan menjadi salah satu sumber protein nabati yang terbuat dari kedelai. Kedelai mengandung senyawa isoflavon glikosida yang bermanfaat untuk kesehatan sebagai pencegah penyakit kardiovaskuler, kanker, diabetes, hipertensi, osteoporosis, obesitas dan menopause. Isoflavon glikosida memiliki struktur yang lebih kompleks, sehingga perlu mengalami proses biokimiawi untuk menyederhanakan strukturnya agar mudah diserap oleh tubuh. Isoflavon glikosida akan disederhanakan menjadi bentuk aglikon selama proses fermentasi dan hidrolisis kimiawi. Genistein merupakan salah satu bentuk isoflavon aglikon yang banyak ditemui pada produk kacang-kacangan. Susu kedelai dan tempe merupakan produk olahan dari kedelai, perbedaannya adalah tempe telah mengalami proses fermentasi, sehingga diyakini memiliki kadar isoflavon aglikon yang tinggi. Penelitian ini bertujuan untuk menentukan kadar genistein pada ekstrak susu kedelai dan tempe dengan menggunakan spektrofotometri UV-Vis. Sampel diekstraksi dengan menggunakan etil asetat, sehingga diperoleh ekstrak kental, selanjutnya diukur kadar genistein dengan metode spektrofotometri UV-Vis, menggunakan baku standar. Hasil penelitian diperoleh kadar genistein pada ekstrak susu kedelai dan tempe yaitu masing-masing 0.613 dan 5.519% (b/b). Hal ini menunjukkan bahwa kadar isoflavon aglikon lebih tinggi pada tempe dibanding susu kedelai.Kata kunci: genistein, isoflavon aglikon, susu kedelai, tempe AbstractSoymilk and tempeh are a processed food that is popular with Indonesian society and becomes one of vegetable protein source made from soybean. Soy contains isoflavones glycosides are useful for health as preventions of cardiovascular disease, cancer, diabetes, hypertension, osteoporosis, obesity, and menopause. Isoflavones glycosides have more complex structures, so they need to undergo biochemical processes to simplify their structure and are easily absorbed by the body. Isoflavones glycosides will be simplified into aglycone forms during chemical fermentation and hydrolysis processes. Genistein is one form of isoflavone aglycone that is commonly found in nuts products. Soymilk and tempeh are processed products from soybeans, the difference is tempeh has undergone a fermentation process, so it is believed to have high levels of isoflavone aglycone. The purpose of this study was to determine the genistein content in the extract of soymilk and tempeh by using UV-Vis spectrophotometry. The sample was extracted by using ethyl acetate, so obtained by viscous extract, then measured its genistein level by UV-Vis spectrophotometry method, using standard reference. The result showed that genistein content of soymilk and tempeh extract were 0.613 and 5.519% (w/w), respectively. This indicates that isoflavone aglycone content is higher in tempeh than soymilk.Keywords: genistein, isoflavone aglycone, soymilk, tempeh
PENGUKURAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lam.) MENGGUNAKAN METODE FRAP (Ferric Reducing Antioxidant Power) St Maryam; Muzakkir Baits; Ainun Nadia
Jurnal Fitofarmaka Indonesia Vol 2, No 2 (2015): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.052 KB) | DOI: 10.33096/jffi.v2i2.181

Abstract

Measurement of antioxidant activity of ethanol extract of Marunggai leaves (Moringa oleifera Lam) was conducted. The aim research to obtain antioxidant activity using FRAP (Ferric Reducing Antioxidant Power). Marunggai leaves (Moringa oleifera Lam.) ware extracted using maceration method by ethanol 96 %. Free radical activity absorbance was measured with a spectrophotometer at a wavelength of 720 nm and the total value of antioxidant activity was calculated based on the data absorbance. The calculations showed that Marunggai leaves (Moringa oleifera Lam.) have the antioxidant activity with the value 7,923 mg AAE/g extract.
PERBANDINGAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) BERDASARKAN TEMPAT TUMBUH DENGAN METODE PEREDAMAN DPPH Aminah Aminah; St Maryam; Muzakkir Baits; Ummi Kalsum
Jurnal Fitofarmaka Indonesia Vol 3, No 1 (2016): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1248.635 KB) | DOI: 10.33096/jffi.v3i1.175

Abstract

Antioxidant activity assay has been conducted in order to determine the comparison of the antioxidant activity contained in the ethanol extract of the soursop leaves (Annona muricata L.) from Makassar, north Mamuju and Jeneponto. The amount of antioxidant activity obtained by testing against free radical DPPH (1,1-Diphenyl-2-picryl Hydrazyl) at concentrations of 10, 20, 30, 40 dan 50 ppm with 96% ethanol. The testing was done with the addition of 1 mL of the test solution with 4 mL DPPH 35 ppm. The absorbance of free radical activity was measured by UV-Vis spectrophotometer at the wavelength 515 nm and the IC50 value was calculated based on the absorbance data. The calculations showed that the soursop leaves from Makassar, North Mamuju, and Jeneponto had the IC50 value of 1.380 µg/mL, 1.512, and 1.420 respectively. The results showed that the samples came from Makassar had a higher antioxidant activity.
UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN TOMAT BUAH (Lycopersicon esculentum Mill, var. pyriforme Alef) DAN DAUN TOMAT SAYUR (Lycopersicon esculentum Mill, var. commune Bailey) DENGAN METODE DPPH (1,1-Diphenyl-2-Picryl Hydrazil) Mamat Pratama; Muzakkir Baits; Rizky Nurul Yaqin
Jurnal Fitofarmaka Indonesia Vol 2, No 1 (2015): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.451 KB) | DOI: 10.33096/jffi.v2i1.183

Abstract

Antioxidant are chemical compounds that can donate one or more electrons to free radicals, so that the free radical reaction is inhibited. Generally, the compounds that have potential as an antioxidant are flavonoids, phenolics and alkaloids. Leaves of tomato (Lycopersicon esculentum Mill.) are plants that contain alkaloid. This study aims to determine the antioxidant activity of the ethanol extract of leaves of fruit tomato (Lycopersicon esculentum Mill, var. pyriforme Alef.) and leaves of vegetable tomato (Lycopersicon esculentum Mill, var. commune Bailey.) with DPPH method (1,1-Diphenyl-2-Picryl Hydrazil). Ethanol extract is obtained by extracting the leaves of friut tomato powder (Lycopersicon esculentum Mill, var. pyriforme Alef.) and the leaves of vegetable tomato (Lycopersicon esculentum Mill, var. commune Bailey.) with maceration. Antioxidant activity was measured using a UV-Vis spectrophotometer with maximum absorption wavelength is 514 nm. Readable absorbance is used to calculate % inhibition and IC50. The results showed that the samples of the leaves of fruit tomato (Lycopersicon esculentum Mill, var. pyriforme Alef.) and leaves of vegetable tomato (Lycopersicon esculentum Mill, var. commune Bailey.) have antioxidant activity with IC50 values of each of 279.482 µg/mL and 280.190 µg/mL. This shows the ethanol extract of leaves of fruit tomato (Lycopersicon esculentum Mill, var. pyriforme Alef.) and leaves of vegetable tomato (Lycopersicon esculentum Mill, var. commune Bailey.) has a very weak activity as an antioxidant.
POTENSI DAUN PALA (Myristica fragrans Houtt) SEBAGAI SUMBER FENOLIK Muammar Fawwaz; Siti Nurdiansyah A; Muzakkir Baits
Jurnal Fitofarmaka Indonesia Vol 4, No 1 (2017): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (815.364 KB) | DOI: 10.33096/jffi.v4i1.230

Abstract

Phenolic is a compound that having an aromatic ring with one or more hydroxyl groups. Phenolic compounds in foods can be grouped into simple phenols and folic acid. This study aimed to determine the total phenolic content of the leaf extract of nutmeg (Myristica fragrans Houtt). Leaves of nutmeg extracted using 70% ethanol, then the extract conducting qualitative analysis by reaction with FeCl3 as specific reagent. The result of this reaction is green solution, it means there are phenolic compound in the extract. The quantitative process of total phenolic was analyzed by UV-Vis spectrophotometry. A comparison used in this analysis is gallic acid; it is a standard reference in the determination of total phenolic content. Sample measured at a wavelength of 860.7 nm, which is the maximum wavelength of gallic acid. Total average content of phenolic is 183.56-mgGAE/g extract. The ethanol extract of nutmeg’s leaves have high total phenolic content, which means it is potential as antioxidant.
ISOLASI DAN IDENTIFIKASI BEBERAPA KOMPONEN KIMIA EKSTRAK DIETIL ETER STANDAR HERBA Oxalis corniculata L. Herwin Herwin; Rachmat Kosman; Muzakkir Baits
As-Syifaa Jurnal Farmasi Vol 6, No 1 (2014): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.346 KB) | DOI: 10.33096/jifa.v6i1.27

Abstract

A research have been conducted about isolation and identification some chemicals components in diethyl ether standard ekstract of Oxalis corniculata L. herb from Mamuju of West Sulawesi with purpose isolation and identification some chemicals components standard ekstract diethyl ether Oxalis corniculata L. herb based on fitochemistri parameters on the way kualitatife dan kuantitatife.  Based on isolation with fitochemistri parameters of kuantitatife that standard diethyl ether ekstract 38 gram. Based on isolation and identification some chemical components obtained that dietyl ether ekstract of thin layer cromatography obtained chemicals components flavanoid at Rf 0.47 value with reagent Antimo (III) cloride, components alcaloids at Rf 0.86 value with reagents chemicals dragendorf, components chemicals steroids at Rf 0.90 with reagents chemicals Liebermann-Bouchard and kualitatife analisis at specific parameter in organoleptic that colour ekstract green, aromatis, smell, a tick form idication obtained alcaloids, steroids and flavanoids.
ANALISIS KADAR PROTEIN DAN LEMAK PADA IKAN JULUNG-JULUNG ASAP (Hemiramphus far) ASAL KECAMATAN KAYOA MALUKU UTARA DENGAN METODE KJELDAHL DAN GRAVIMETRI Mamat Pratama; Muzakkir Baits; Nurul Auliah A.R. Saman
As-Syifaa Jurnal Farmasi Vol 6, No 2 (2014): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.767 KB) | DOI: 10.33096/jifa.v6i2.47

Abstract

Content Analysis of Protein And Fats In fish smoked julung-julung (Hemiramphus far) Origin of North Maluku, Subdistrict Kayoa with Kjeldahl Method and Gravimetri Method. An investigation of analysis of protein and fat content in fish smoked julung-julung (Hemiramphus far) Origin Of North Maluku,District kayoa with kjeldahl method and gravimetri method. This study aimed to obtain scientific data about the content of protein and fat in fish smoked julung-julung (Hemiramphus far).This includes research on the protein nitrogen content of the test by Kjeldahl Method and analysis of fat content by using the method showed that the levels Gravimetri. The result showed that the samples of smoked fish fated to be unlucky-fated to unlucky (Hemiramphus far) obtained A water content of 13,90%, protein content of 71,03% and fat content 4,13% and in fish simple fated to be unlucky-fated to unlucky (Hemiramphus far) obtained B water content of 14,07%, protein content of 71,67% and fat content 5,38%.Keywords : Smoked fish julung-julung, protein, fat, kjeldahl and gravimetri.
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN TALAS KETAN (Colocasia esculenta) TERHADAP BAKTERI Saphylococcus aureus DAN Salmonella thypi SECARA DIFUSI AGAR Herwin Herwin; Muzakkir Baits; Ririn Ririn
As-Syifaa Jurnal Farmasi Vol 8, No 1 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.954 KB) | DOI: 10.33096/jifa.v8i1.164

Abstract

Antibacterial activity of taro leaves ekxtract has been conducted. The Recearch aimed to determine the antibacterial activity and concentration of ethanol extract of taro leaves (Colocacia esculeta) inhibited the growth of either Salmonella thypi and Staphylococcus aureus by agar diffusion. The result of the antibacterial activity screening in concentration of 1% showed that the ethanol extract of taro leaves by solid dilution provided activity againts Salmonella thypii and Staphylococcus aureus. Based on the test results of antibacterial activity by agar diffusion using concentration of 1% , 2% and 4 % showed that the ethanol extract of taro has the largest inhibition zone diameter againts Staphylococcus aureus of 4% was 15 mm.
AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN FRAKSI DAUN Colocacia esculenta L. DENGAN METODE KLT-BIOAUTOGRAFI DAN DIFENILPIKRIL HIDRAZIL Herwin Herwin; Muzakkir Baits; Ririn Ririn
As-Syifaa Jurnal Farmasi Vol 7, No 2 (2015): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.392 KB) | DOI: 10.33096/jifa.v7i2.9

Abstract

The aim of the research to determine the antibacterial and antioxidant activity of Colocacia esculenta fraction leaves derive from Pare-Pare South Sulawesi in using TLC- Bioautography and diphenilpycril Hydroxil. Screening antibacterial activity test of the Colocacia esculenta fraction leaves exhibited in concentration 1% active on pathogenic bacteria Salmonella thypi. Based on either antibacterial and antioxidant activities by TLC-Bioautography showed that fraction at Rf 0.70 value active on bacteria Salmonella thypi and antioxidant active as well. Identification of active chemical compound showed flavonoid compound using Iodium-KI and antimo (III) clorida as reagent.
Tyrosinase Inhibitor Activity Measurement of Crude and Purified Extract of Moringa Leaves (Moringa oleifera L.) Zainal Abidin; Ummu Khaeriah; Zuhrina Zuhrina; Mamat Pratama; Muzakkir Baits
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 2, No. 1 (2019)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.756 KB) | DOI: 10.24198/ijpst.v1i1.19152

Abstract

Moringa leaves is one of the plants that have antioxidant activity. The compounds that have strong antioxidant activity also have strong antityrosinase activity. Moringa leaves contain flavonoid compounds that can inhibit tyrosinase activity in the melanin formation process. This study aimed to determine tyrosinase inhibitor activity of crude and purified extract of moringa leaves (Moringa oleifera L.). Tyrosinase inhibitory activity measurement was done by in vitro studies with measuring dopachrome from the oxidation of L-tyrosine in the mechanism of melanogenesis. The crude extract obtained by use maceration extraction method and ethanol solvent, whereas the purified extract obtained by use liquid-liquid extraction method and ethyl acetate solvent. The results showed that hydroquinone has IC50 value of 18,234 μg/mL while crude extract of moringa leaves shows IC50 value of 4405,24 μg/mL and purified extract of moringa leaves shows IC50 value of 401,6228 µg/mL. Purified extract of moringa leaves more active as tyrosinase inhibitor than its crude extract but less active than hydroquinone.Keywords: Moringa leaves, Purified extract, Tyrosinase Inhibitor, UV Rays