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Characterization of Full Length Sequence of JJ3 Isolated from Shade Tolerant Soybean Nurul Khumaida; , Kisman; Didy Sopandie
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 2 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.794 KB) | DOI: 10.24831/jai.v36i2.13696

Abstract

The aim of this study was to characterize full length sequence of JJ3 isolated from shade tolerant soybean. The characterization of full length sequence involved analyses of structure of full length sequence, open reading frame (ORF) or coding sequence (CDS), and homology. The analyses of JJ3 full length sequence structure were done using GeneScanW program, coding sequence (CDS) or open reading frame (ORF) using ORF Finder program, and the homology of JJ3 full length sequence were done using BLAST and clustalW programs. Results showed that the full length sequence of JJ3 consisted of single exon and polyA. Single exon was a coding sequence (CDS) 633 bp produced open reading frame (ORF) with 210 amino acid deduction comprised of 136 conserved amino acids and 77 varied amino acids. The conserved domain of JJ3 produced functional protein, PsaD photosystem I (PSI) subunit, related to photosynthetic transport electron in PSI reaction center. The full length of JJ3 homolog to psaD, a gene encoding protein subunit photosystem I (PSI) related to mechanism of plant adaptation to shade stress, in wood tobacco (Nicotiana sylvestris), rice (Oryza sativa), tomato (Lycopersicon esculentum), barley (Hordeum vulgare), spinach (Spinacia oleracea), Arabidopsis (Arabidopsis thaliana), and potato (Solanum tuberosum).Key words: full length sequence, gene, homology, shade tolerant, soybean
Penampilan Vegetatif Tiga Genotipe Kedelai Berbiji Besar Pada Kondisi Stres Genangan Kisman Kisman; Farid Hemon; Sumarjan Sumarjan; Suprayanti Dewi
JURNAL SAINS TEKNOLOGI & LINGKUNGAN 2021: Special Issue, Oktober 2021
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v0i0.266

Abstract

ABSTRACTThis study aims to determine the vegetative appearance of three genotypes of large-seeded soybean under puddle stress. The Experimental method was used and the plastic buckets as the experimental units were arranged using a Split Plot Design. The main plot was the stress factor (S) consisting of normal conditions (S0) and puddle stress (S1), and the subplot was the genotype factor (G) consisting of G1 (KH1), G2 (Argomulyo), and G2 (Grobogan). Each genotype was repeated four times. The observed variables were plant height, number of leaves, leaf area, number of productive branches, specific leaf weight, root/shoot dry weight ratio. The results showed that there were significantly differences in the appearance of the vegetative characters of the three genotypes of large seeded soybean under puddle stress conditions, especially on plant height, leaf area, plant dry weight, and shoot dry weight. Under puddle stress conditions, KH1 showed the highest of the plant height, root dry weight, and shoot dry weight. Argomulyo showed the highest of the leaf area, while Grobogan did not show as a tolerant variety under puddle stress conditions. 
PENGARUH KOMPOSISI MEDIA TANAM ARANG SEKAM TERHADAP KARAKTER PERTUMBUHAN DAN HASIL BEBERAPA GENOTIP TANAMAN OKRA (Abelmoschus esculentus L.) Hulya Hat taya; A Farid Hemon; Kisman *3
CROP AGRO, Scientific Journal of Agronomy Vol 12 No 2 (2019): Jurnal Crop Agro Juli 2019
Publisher : Department of Agronomy Faculty of Agriculture University of Mataram and Indonesian Society of Agronomy Branch NTB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.567 KB) | DOI: 10.29303/caj.v12i2.266

Abstract

Okra (Abelmoschus esculentus L.) is one of the important vegetable crops. In its growth, okra plant is influenced by genetic and environmental factors especially planting media. This study aimed to determine the effect of composition of husk charcoal planting media on growth and yield characters of several okra genotypes. This study has been carried out in the greenhouse of Agriculture Faculty of Mataram University. The experiment was arranged in a factorial complete randomized design (CRD) consisting of two factor, namely the composition of planting media and genotype. The composition of planting media factor consist 0f 6 treatments while the genotype factor consist of 3 treatments. Every treatment combination was replicated three times so there were 54 plants (experimental unit). The research data were analized using analysis of variance at the 5% level of significant. Significant result were tested further with a honestly significant difference (HSD) method. Result of this study showed that interaction composition of planting media with genotype and the composition of planting media did not influence significantly all parameters. Genotype factor had affect significant on plant height 60 and 90 days after transplanting, flowering date, number of flower, number of flower become fruit, number of fruit suitable for comsumption, and length of fruit suitable for comsumption. The fastest growth among the genotype was Dompu genotype and the highest yield was Red genotype.
SOSIALISASI PENGEMBANGAN PERTANIAN LAHAN KERING DAN PEMANFAATAN AIR TANAH DALAM DI DESA SALUT KECAMATAN KAYANGAN, KABUPATEN LOMBOK UTARA I Dewa Gede Jaya Negara; Bambang Hari Kusumo; Kisman Kisman; Tajidan Tajidan; Lolita Endang Susilawati; Bambang Budi Santoso; Joko Priyono
Jurnal Abdi Insani Vol 9 No 2 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i2.575

Abstract

The potential for dry land in North Lombok is very wide with the surrounding rivers mostly dry during the dry season. Sambik Rindang Hamlet is one of the dry lands that has the potential for productive agriculture, but has not been able to be utilized optimally. For this reason, the people of Sambik Rindang need to be given knowledge about this through socialization, so that they can make efficient use of dry land and groundwater so that farming can be carried out outside the rainy season. The aim of the socialization is to increase people's knowledge about irrigation water efficiency and irrigation methods for agriculture in dry land. This socialization was carried out with the stages of field review, field socialization, discussion and question and answer, evaluation and closing. Furthermore, this socialization service has succeeded in providing knowledge about efficient irrigation such as drip irrigation and pipelines, with productive farming. The service participants were very enthusiastic about participating in the socialization activities, questions about agriculture and irrigation were answered well by the team from the Faculty of Agriculture and the Faculty of Engineering. Evaluation of activities is carried out by the service team, from the field socialization activities to the end of the activity. The service participants were very proactive during the activity and had knowledge about efficient irrigation and productive farming methods. Thus the service participants have known efficient irrigation methods and have gained knowledge in utilizing dry land in order to have an economic impact on the family.
Pendampingan Kelompok Penangkar Benih Kedelai Binaan UD. Humfik Tani Untuk Meningkatkan Ketersediaan Benih Kedelai Bersertifikat Kisman; Uyek Malik Yakop; A. Farid Hemon; B. Erna Listiana; Suprayanti Martia Dewi
Jurnal SIAR ILMUWAN TANI Vol. 1 No. 2 (2020): Jurnal Siar Ilmuwan Tani
Publisher : Fakultas Pertanian Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.35 KB)

Abstract

UD. Humfik Tani is one of the seed producers of "Rice and Soybean" which has many assisted seed breeders in Central Lombok. However, until now seed breeders have not been able to provide and procure soybean seeds as needed. The purpose of this community service activity was to provide theoretical and technical assistance to the soybean seed breeder group guided by UD. Humfik Tani in producing certified soybean seeds to increase the availability of certified soybean seeds. The method used in this service activity was the Adult Education (POD) or Androgogy method by emphasizing the active participation of participants, group work and field demonstrations. This mentoring activity was carried out in the form of counseling and field education (demonstration plots) which are conducted in Ubung village, Jonggat district. This activity was attended by 14 participants (UD. Humfik Tani, fostered breeders, UPT BPP, PPL, Tani Tunggal Angen). The results shows that 1) This service activity was going well and the participants were very earnest and enthusiastic about all activities ranging from counseling to visits to the demonstration plot location and were very interested in Biosoy 2 large seed soybeans. 2) UD. Humfik Tani was very interested and will develop Biosoy 2 certified soybean seeds in his breeding land and the breeders at MK II August 2020 to provide seed needs on MH December 2020
Upaya Peningkatan Produksi Kentang Melalui Penggunaan Benih Bersertifikasi di Kawasan Sembalun, Kabupaten Lombok Timur Muhammad Sarjan; Kisman Kisman; Farid Hemon
Jurnal Pengabdian Magister Pendidikan IPA Vol 3 No 1 (2020)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.954 KB) | DOI: 10.29303/jpmpi.v3i1.400

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Melalui kegiatan pengabdian pada Masyarakat ini Perguruan Tinggi ingin membantu diseminasi produksi benih kentang yang bermutu bersertifikat  dan menjadikan kelompok tani  sebagai  penangkar benih (Seed Grower) yang handal secara berkelanjutan. Keberadaan penangkar benih ini diharapkan dapat mensuplai benih pada budidaya kentang khususnya  ditingkat lokal kawasan Sembalun . Adanya penangkar Benih kentang  ini sekaligus dapat meningkatkan income generating dari Kelompok Petani penangkar dan permasahan penyediaan benih bermutu untuk petani di Kawasan Sembalun khususnya dan di Kabupaten Lombok Timur pada umumnya dapat diatasi. Selanjutnya penggunaan benih bermutu bersertifikasi  diharapkan juga dapat meningkatkan produksi kentang dan akhirnya dapat meningkatkan pendapatan petani.  Berdasarkan hasil kegiatan dan evaluasi yang dilakukan antara lain diperoleh bahwa Penyuluhan yang diikuti dengan demonstrasi Plot budidaya benih kentang bersertifikat  bagi Masyarakat Kelompok Tani Calon Penangkar Benih  di Desa Sembalun Bumbung- Lombok Timur dapat meningkatkan minat, pengetahuan dan keterampilan masyarakat petani dalam pengembangan benih Kentang bersertifikat, sehingga produksi dan kualitas kentang  yang dihasilkan menjadi lebih baik. Masyarakat desa Sembalun Bumbung  mempunyai motivasi yang tinggi untuk mengusahakan tanaman kentang termasuk minat yang cukup tinggi untuk menjadi penangkar benih kentang.  Untuk keberlanjutan usaha perbenihan disarankan antara lain perlunya pengawasan dan bimbingan yang terus menerus kepada petani penangkar benih kentang di desa Sembalun Bumbung terutama dari pihak terkait seperti BPSB sehingga kelas atau mutu dan kualitas benih kentang yang dihasilkan menjadi lebih baik dan lebih profesional sebagai penagkar benih. Disarankan kepada para petani calon penangkar benih kentang agar terus meningkatkan kualitas benih yang dihasilkan dengan cara melengkapi sarana dan prasarana seperti penyiapkan gudang penyimpanan benih.Kata Kunci:  Benh Kentang; sertifikat;  Penangkar; Sembalun
INFEKSI CENDAWAN SCLEROTIUM ROLFSII PADA KACANG TANAH YANG DITANAM PADA KONDISI CEKAMAN KEKERINGAN A. Farid Hemon; M. Sarjan; Kisman (3
AGROTEKSOS, Jurnal Ilmiah Ilmu Pertanian Vol 26 No 1 (2015): Jurnal Agroteksos 3 Desember 2015
Publisher : Fakultas Pertanian Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.7 KB)

Abstract

ABSTRAK Cendawan S. rolfsii merupakan patogen dominan yang menyerang kacang tanah terutama di lahan kering. Pengaruh dua cekaman (kekeringan dan infeksi S. rolfsii) pada tanaman kacang tanah belum banyak informasinya. Oleh karena itu, penelitian ini betujuan untuk mengetahui tingkat infeksi cendawan Sclerotium rolfsii pada berbagai cara inokulasi pada kondisi cekaman kekeringan dan perilaku tanaman kacang tanah (pertumbuhan dan daya hasilnya). Penelitian ini telah dilaksanakan di laboratorium Mikrobiologi dan rumah kaca Fakultas Pertanian Universitas Mataram dengan menggunakan Rancangan Acak Lengkap-Faktorial, dengan perlakuan : cekaman kekeringan (C) dan cara inokulasi (I). Faktor cekaman kekeringan (C) terdiri dari: C1 = kondisi lengas tanah kapasitas lapang dan C2 = kondisi cekaman kekeringan. Faktor cara inokulasi (I) terdiri dari: I0 = tanpa infeksi S. rolfsii, I1 = inokulasi S. rolfsii dengan cara menempel langsung biakan pada pangkal batang tanpa dilukai, I2 = inokulasi S. rolfsii dengan cara menempel langsung biakan pada pangkal batang yang telah dilukai, dan I3 = inokulasi S. rolfsii dengan cara menabur biakan pada media tanah. Hasil penelitian menunjukkan bahwa cara inokulasi dengan cara menabur suspensi langsung ke media tanah dapat meningkatkan tingkat infeksi cendawan Sclerotium rolfsii terutama pada kondisi cekaman kekeringan. Akibat infeksi patogen tersebut menyebabkan tinggi tanaman, jumlah daun, jumlah cabang dan jumlah polong menjadi lebih terhambat pertumbuhannya. ABSTRACT Sclerotium rolfsii fungi is dominant patogen attacking peanut plant in dry land. The study of double stress effect (drought and S. rolfsii infection) to peanut plant is still limited its information.Therefore, this research aimed to know S. rolfsii infection severely on inoculation method under drought stress and peanut plant character (growth and yield of peanut plant). This research had been done in Microbiology laboratory and Green House Faculty of Agriculture Mataram University with Completely Randomized Design-Factorial (treatments : drought stress = C and inoculation method = I). Factor of drought stress consisted of water sufficient (C1) and drought stress (C2). Factor of inoculation method consisted of no infection S. rolfsii (I0), inoculation with patch of S. rolfsii isolate on base stem without injured plant stem (I1), inoculation with patch of S. rolfsii isolate on base stem with injured plant stem (I2), and inoculation with spread of S. rolfsii isolate on soil medium (I3).Result showed that inoculation method with spread of S. rolfsii isolate suspention on soil medium increased infection severely of S. rolfsii fungi under drought stress. Infection of S. rolfsii fungi reduced plant height, leaf number, branch number and pod number of peanut plant.
PENGARUH PENERAPAN PAKET TEKNOLOGI BUDIDAYA TERHADAP KARAKTER PERTUMBUHAN DAN HASIL GENOTIPE TANAMAN JAGUNG (Zea mays L.) Kisman kis man
AGROTEKSOS, Jurnal Ilmiah Ilmu Pertanian Vol 30 No 1 (2020): Jurnal Agroteksos April 2020
Publisher : Fakultas Pertanian Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.376 KB) | DOI: 10.29303/agroteksos.v30i1.590

Abstract

ABSTRAK Jagung (Zea mays L.) merupakan salah satu komoditas strategis dalam pembangunan pertanian dan perekonomian Indonesia. Jagung tidak hanya diperlukan untuk pangan, namun juga lebih banyak dibutuhkan untuk pakan dan bahan baku industri dalam jumlah yang terus meningkat. Untuk memenuhi kebutuhan permintaan jagung, peningkatan produksi masih perlu diupayakan. Penerapan teknologi budidaya merupakan salah satu inovasi yang dapat dilakukan untuk meningkatkan produksi jagung, sehingga dapat memenuhi kebutuhan permintaan jagung. Penelitian ini bertujuan untuk mengetahui pengaruh penerapan paket teknologi budidaya terhadap karakter pertumbuhan dan daya hasil genotipe tanaman jagung (Zea mays L.). Metode penelitian yang digunakan adalah metode eksperimental dengan percobaan lapang. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK), dengan 4 perlakuan teknologi budidaya (T) : yaitu T1=Varietas Sukmaraga dengan jarak tanam (35x35)x100) cm. T2=Populasi C2 dengan jarak tanam 70 x 20 cm. T3=Lokal Bima dengan jarak tanam 70 x 20 cm. T4=Varietas Lamuru dengan jarak tanam (40x30)x60) cm. Masing-masing perlakuan diulang 6 kali sehingga diperoleh 24 unit percobaan. Data hasil pengamatan dianalisis dengan analisis ragam pada taraf 5% dan dilanjutkan dengan Uji Beda Nyata Jujur (BNJ) pada taraf yang sama. Hasil penelitian menunjukkan bahwa: Penerapan paket teknologi budidaya T1 dan T4 secara statistik memberikan hasil sama atau menunjukkan tidak berbeda nyata terhadap hasil berat kering pipil (gr/tan) serta lebih baik dibandingkan dengan perlakuan paket teknologi T2 dan T3
KERAGAAN HASIL DAN KOMPONEN HASIL BEBERAPA GALUR KEDELAI BIJI COKLAT PADA KONDISI CEKAMAN NAUNGAN Kisman .; I Wayan Sutresna; IGP Muliarta A.; Idris .; AAK Sudharmawan
AGROTEKSOS, Jurnal Ilmiah Ilmu Pertanian Vol 26 No 1 (2016): jurnal agroteksos 1 April 2016
Publisher : Fakultas Pertanian Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.28 KB)

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui keragaan hasil dan komponen hasil beberapa galur kedelai biji coklat hasil persilangan kedelai hitam x kedelai kuning pada kondisi cekaman naungan. Metode yang digunakan dalam penelitian ini adalah metode eksperimental dengan percobaan lapang menggunakan pot percobaan. Rancangan yang digunakan adalah Rancangan Acak Lengkap dengan dua faktor: faktor genotype (G) yang terdiri atas tujuh galur kedelai biji coklat, hasil persilangan tetua kedelai hitam (Ceneng, Detham-1) dengan kedelai kuning (Lokal Godek, Burangrang), dan faktor naungan (N) yang terdiri atas naungan 65% dan tanpa naungan. Masing-masing perlakuan diulang tiga kali sehingga terdapat 42 pot percobaan. Hasil penelitian menunjukkan bahwa: (1). Keragaan hasil dan komponen hasil galur kedelai biji coklat mengalami perubahan (penurunan) hingga 50% pada kondisi naungan 65% dibandingkan pada kondisi tanpa naungan. (2). Galur G1, G2, G3, dan G4 hasil persilangan Ceneng x Lokal Godek dan Ceneng x Burangrang tergolong tahan terhadap naungan 65%, sedangkan G5, G6, dan G7 hasil persilangan Detham-1 x Lokal Godek dan Detham-1 x Burangrang tergolong rentan atau peka terhadap cekaman naungan 65%. ABSTRACT The objective of this study was to know the performance of yield and yield components of brown seeded soybean lines under shade stress. The experimental method and Completely Randomized Design with two factors were used in this study. These two factors were 7 lines of brown seeded soybean and shading factors. Each of combination factors were in three replicates. The results of this study showed that: (1). Performance of yield and yield components of brown seeded soybean lines changed (decreased about 50%) under 65% shading compare to no shading. (2). Four lines (G1, G2, G3, G4) were relatively tolerant to shade stress, while G5, G6, and G7 were relatively susceptible to shade stress.
Elementary School Student Learning Independence Analyst during the Covid-19 Pandemic Kisman Kisman; A. Hari Witono; Moh. Irawan Zain
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol 9, No 2: December 2021
Publisher : IKIP Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (731.765 KB) | DOI: 10.33394/j-ps.v9i2.4367

Abstract

Independent learning is an important element in learning. Independent learning is defined as a learning process within a person in achieving certain goals that are required to be active individually or not to depend on others, including teachers. This study aims to find out how independent student learning is during the Covid-19 pandemic and the factors causing difficulties in student learning independence during the Covid-19 pandemic class V SD Negeri 46 Mataram. The research approach used is descriptive qualitative research. The data collection instruments used interview questionnaires, observation sheets, and documentation studies. The data analysis technique used is data reduction, data presentation, conclusion or verification. The results show that the learning independence experienced by class V students includes: (1) aspects of having self-confidence reaching an average percentage of 50.3%, (2) aspects in making decisions reached an average percentage of 38.6%, (3) responsible aspects reached an average percentage of 50%, (4) aspects of having a competitive desire to advance reached an average percentage of 44.6%, (5) the discipline aspect reached an average percentage of 41.5%. Then the student's learning independence factor in terms of self-confidence, the average percentage is 44.33%. Then the student's learning independence factor in a decision, the average percentage is 36.66%. Then the independent learning factor of students in terms of responsibility, the average percentage is 45.75%. Then the student's learning independence factor in terms of the desire to compete to advance, the average percentage is 38.66%. Then the student's learning independence factor in terms of discipline, the average percentage is 50%