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All Journal Jurnal Akademika Biologi Biology, Medicine, & Natural Product Chemistry ZOO INDONESIA Zoo Indonesia Prosiding Konferensi Nasional PKM-CSR Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Biosaintifika: Journal of Biology & Biology Education
Agung Budiharjo
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Medicine & Pharmacology Nursing Public Health Social Sciences Other

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High Performance Liquid Chromatography (HPLC) for Detection of Glucosamine and Chondroitin Sulfate Compounds Rakhmiyati, Rakhmiyati; Widiyani, Tetri; Budiharjo, Agung
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.5-8

Abstract

Glucosamine and chondroitin sulfate are compounds found in shark cartilage (Carcharhinus sorrah). The two compounds have many health benefits, that is wound healing and helping the process of angiogenesis. This study aims to determine the content of glucosamine and chondroitin sulfate compounds in shark cartilage (SC) extract. The method used was High Performance Liquid Chromatography (HPLC) with potassium phosphate buffer solution at pH 3. The results of this research were SC extract contained glucosamine and chondroitin sulfate compounds with a retention time of 1.914 minutes.
Fish Diversity and Phylogenetics in the Lakes of Bromo Tengger Semeru National Park Based on eDNA Chuanata, Wilsen; Susilowati, Ari; Budiharjo, Agung; Travis, Justin M. J.
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22842

Abstract

Indonesia is home to 1,248 species of freshwater fish, which play a vital role in sustaining the ecosystem. Lakes Ranu Pani, Ranu Regulo, and Ranu Darungan, located within the Bromo Tengger Semeru National Park, lack data on freshwater fish. This study aimed to assess fish diversity in these lakes using environmental DNA (eDNA) metabarcoding and to establish phylogenetic relationships among the species present. Water samples were filtered using 0.45 µm mixed cellulose ester filter paper, after which the collected DNA was extracted utilizing a DNA miniprep kit. The 12S rRNA gene was amplified using the MiFish primer, and the amplicon DNA was sequenced with the Illumina MiSeq 2x250 platform. The sequences were aligned using Blast+ for species identification and subsequently analyzed for alpha and beta diversity. A phylogenetic tree was constructed with MEGA12. The findings revealed eight species from five families, with Gambusia affinis being the most abundant. Ranu Pani exhibited low species diversity, while Ranu Regulo and Ranu Darungan displayed moderate diversity. Beta diversity analysis indicated similarities between Ranu Pani and Ranu Regulo, but distinct communities were found in Ranu Darungan. Phylogenetic analysis showed close relationships between species from the Cichlidae and Osphronemidae families, while a more distant relationship was observed with the Cyprinidae family. These baseline data can serve as a reference for future research on developing conservation strategies, monitoring environmental changes, and supporting biodiversity management.
Co-Authors ARI SUSILOWATI Artini Pangastuti Chuanata, Wilsen Dadi Pratama Danang Setyo Pambudi Hasan Ayyubi I Ketut Suada Nayasilana, Ike Nurjuita Nieko Octavi Septiana Okid Parama Astirin Pertiwi, Rizqi Adanti Putri Rakhmiyati, Rakhmiyati Rory Ade Kristiawan S Sugiyarto S Sunarto SHANTI LISTYAWATI Sugiyarto Sugiyarto TETRI WIDIYANI TETRI WIDIYANI Travis, Justin M. J.