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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Kedokteran Brawijaya Jurnal Kedokteran Diponegoro The Indonesian Biomedical Journal Jurnal Epidemiologi Kesehatan Komunitas Care : Jurnal Ilmiah Ilmu Kesehatan Medica Hospitalia Indonesian Journal of Cancer
Selamat Budijitno
Staf Pengajar Bagian Bedah FK Undip Semarang
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Indonesian Journal of Cancer

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Synergistic Cytotoxicity of 5-Fluorouracil and Epigallocatechin-3-Gallate on Colorectal Cancer Stem Cell Ibrahim, Sugeng; Riwanto, Ignatius; Suharti, Catharina; Putra, Agung; Budijitno, Selamat
Indonesian Journal of Cancer Vol 18, No 2 (2024): June
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v18i2.1211

Abstract

Background: Colorectal cancer stem cells (CR-CSCs) derived from the HCT-116 cell line established human colon carcinoma cell line, validated with CD44+/CD133+. The study investigates the synergistic effects of combining epigallocatechin gallate (EGCG) with 5-fluorouracil (5-FU) on CR-CSCs through comprehensive cytotoxicity assessments, aiming to enhance therapeutic outcomes. EGCG is a polyphenol with anti-cancer activity in green tea. Previous studies have reported that the anti-cancer activity of EGCG involves inhibition of proliferation and induction of apoptosis thereby reducing recurrence by as much as 51.6% in patients with colorectal adenoma after polypectomy. The significance lies in optimizing treatment strategies by understanding the potential synergies between conventional chemotherapeutic agents and natural compounds. Given 5-FU's status as a cornerstone in CR-CSCs chemotherapy and EGCG's emergence as a promising natural compound, the study delves into their individual and combined cytotoxicity profiles. Methods: The single and combination assay aimed to determine the cytotoxicity of EGCG and 5-FU, including establishing the half inhibitory concentration (IC50) and combination index (CI) values. CR-CSCs colonies were disassociated, counted, and cultured in 96-well plates. Test solutions of varying concentrations were applied, and subsequent steps involved incubation, media removal, washing, MTT reagent addition, and absorbance measurement. Results: The single cytotoxicity tests established individual IC50 values, revealing 141.26 µM for 5-FU and 464.56 µM for EGCG. Subsequent combination cytotoxicity tests demonstrated a synergistic effect at specific doses, indicated by CI values below 1. Conclusions: These findings highlight the potential for increased cytotoxicity against CR-CSCs when treated with the combination of 5-FU and EGCG.
Co-Authors Agung Pribadi Agung Putra Alwi, Luqman Ariana, Siwi Catharina Suharti Dwi Sutiningsih Edi Dharmana Edwin Basyar Gery Rifano Hardanto Hardian Hardian Hartanto, Bernardus Ryan Heri Nugroho Hermawan Istiadi Ibrahim, Sugeng Ignatius Riwanto, Ignatius Issakh, Benny Lestariningsih Lestariningsih Malik, Ricat Hinaywan Martha Irene Kartasurya Martini Martini Mateus Sakundarno Adi, Mateus Sakundarno Muniroh, Muflihatul Nani Maharani Neni Susilaningsih Pamungkas, Motris Paulus, Antonio Prabowo, Erik Pranitia, Rainta Putra, Muhammad Reza Tryas Rini, Sulistio Suhartono Suharyo Hadisaputro Syarief Thaufik H, Syarief Udadi Sadhana, Udadi Wibowo, Erwin Winarto Winarto Yan Wisnu Prajoko