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Puspo Edi Giriwono
Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor Jl. Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680, Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Social Sciences

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Journal : Majalah Obat Tradisional

 1 
SENYAWA BIOAKTIF TORBANGUN (Plectranthus amboinicus (Lour.) SPRENG) FRAKSI KLOROFORM INDUKSI APOPTOSIS PADA KANKER PAYUDARA (SEL MCF-7) IN VITRO Wahid Yulianto; Nuri Andarwulan; Puspo Edi Giriwono; Joko Pamungkas
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.642 KB) | DOI: 10.22146/tradmedj.24312

Abstract

Torbangun (Plectranthus amboinicus (Lour.) Spreng) is a medicinal plant that has been traditionally used in tropical countries to cure various illnesses. The objective of this study was to identify the active compounds in the chloroform fraction which have effect on the apoptosis-related genes expression of breast cancer MCF-7 cells. Apoptosis was observed morphologically using Hoechst nuclear staining. Expression of the genes was analyzed using Real-Time PCR. Chemical compounds of the plant fractions were determined using LC-MS. Result of cell morphology observation clearly indicated apoptosis after the treatment of the plant fraction. Increased expression of anti-apoptotic gene Bcl-2 could not prevent the cells from apoptosis. Expressions of p53 and p21 genes were increased significantly. The expressions of caspase 9, caspase 7 and caspase 1 were increased at concentration-dependent manner. Most of the compounds in the chloroform fraction are identified as diterpenoids which may contribute to the apoptosis inducing activity of the fraction.
Co-Authors Afif Arwani Aliwikarta, Kuswanto Andi Nadya Tita Alia Andini Giwang Kinasih Antung Sima Firlieyanti Apon Zaenal Mustopa Apon Zaenal Mustopa Ayu Puspitalena RTR Betty Sri Laksmi Jenie Cesar Welya Refdi Christian Liguori, Christian DAHRUL SYAH Dahrul Syah Dahrul Syah Deddy Muchtadi Dedi Fardiaz Dewi, Fitriya N. Dian Herawati Dias Erfan Dias Indrasti Eko Hari Purnomo Elsera Br Tarigan Elsera Br Tarigan, Elsera Br Endang Prangdimurti Enny Rimita Sembiring Fadly, Khaerul Farida, Eko Feri Kusnandar FITRY TAFZI Fransiska R. Zakaria Fransiska Rungkat FRANSISKA RUNGKAT ZAKARIA FRANSISKA RUNGKAT ZAKARIA Hanifah Nuryani Lioe Harsi D. Kusumaningrum Hartati Chairunnisa Iftita Rakhma Ikrima Indrayana, Stefanus Kartika Sari Kharisma Tamimi Kusmajadi Suradi Kusmajadi Suradi Kuswanto Aliwikarta Lidwina Lidwina Lilis Nuraida Maggy Thenawidjaja Suhartono Masayu Nur Ulfa Nasution, Zuraidah Noviana, Astrid Nugraha Edhi Suyatma Nur Wulandari Nuri Andarwulan Palupi, Nurheni Sri Pamungkas, Joko Purwiyatno Hariyadi Ratna Sari Listyaningrum Reni Koja Rimbawan , Rimbawan Rimbawan Rina Dias Agustin RTR, Ayu Puspitalena Sari Apriliana Satya Nugroho Sentani Chasfila Sri Yadial Chalid Stefanus Indrayana Suyanto Suyanto Tanaka, Darren Wahid Yulianto Wendry Setiyadi Putranto Wihardi, Alfin Maulana Winiati Pudji Rahayu Wiwi Hartuti Zakaria Zakaria