Marry Christiyanto
Program Studi S1 Peternakan Fakultas Peternakan Dan Pertanian, Universitas Diponegoro, Semarang, Jl. Prof. H. Soedarto, S.H, Kota Semarang, Jawa Tengah 50275

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Journal : Animal Agricultural Journal

KECERNAAN BAHAN KERING DAN BAHAN ORGANIK SECARA IN VITRO AMPAS AREN YANG DIFERMENTASI DENGAN PENAMBAHA NITROGEN,PHOSPOR DAN POTASSIUM (The Dry Matter Digestibility and The Organic Matter Digestibility In Vitro of Arenga Pinnata Merr. Fermentated with Pamungkas, Yulianto; Christiyanto, Marry; Subrata, Agung
Animal Agriculture Journal Vol 3, No 2 (2014): Volume 3, Nomor 2, Tahun 2014
Publisher : Animal Agriculture Journal

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ABSTRAKTujuan dari penelitian ini adalah untuk mengetahui KcBK dan KcBO secara in vitro Ampas Aren (AA) yang difermentasi dengan bakteri selulolitik yang berupa MA-11 dengan penambahan unsur Nitrogen (N), Phospor (P) dan Potassium (K). Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor yang diulang 3 kali. Faktor pertama adalah penambahan NPK sebanyak 1,2% bobot/bobot.  Faktor kedua adalah level lama pemeraman (3, 7, 14, 21 hari). Parameter yang diamati adalah KcBK dan KcBO. Hasil penelitian menunjukkan rerata nilai KcBK dan KcBO berturut-turut adalah 27,77% - 46,70% dan 28,64% - 46,70%. Simpulan dari penelitian ini adalah pengolahan AA melalui fermentasi dengan penambahan NPK sebanyak 1,2% bobot/bobot yang dikombinasikan dengan lama pemeraman mampu meningkatkan KcBK dan KcBO serta mempercepat proses fermentasi.Kata kunci: ampas aren; fermentasi; kecernaanABSTRACTThis research was conducted to evaluate in vitro digestibility of Arenga pinnata merr.fermented with cellulolytic bacteria in the form of MA-11 and added of Nitrogen (N), Phosphorus (P) and Potassium (K) like as NPK fertilizer. The research was done with completely randomized design (CRD) factorial with two factor and 3 replications. The first factor is additions of NPK as much as 1,2% W/W. The second factor was level a long time of fermentation (3, 7, 14 and 14 days). The results showed that average of Dry Matter Digestibility (DMD) and Organic Matter Digestibility (OMD) respectively were 27,77% - 46,70% and 28,64% - 46,70%. It can be concluded that processing of Arenga pinnata merr  by fermentation with additions of NPK as much as 1,2% W/W combined a long time  of fermentation can improve DMD and OMD, and accelerate the with time of fermentation process.Keywords: Arenga pinnata merr; fermentation; digestibility
PRODUKSI AMONIA, UNDEGRADED PROTEIN DAN PROTEIN TOTAL SECARA in vitro BUNGKIL BIJI KAPUK YANG DIPROTEKSI DENGAN TANIN ALAMI Jenny, Imelda; Surono, Surono; Christiyanto, Marry
Animal Agriculture Journal Vol 1, No 1 (2012): Volume 1, Nomor 1, Tahun 2012
Publisher : Animal Agriculture Journal

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Ransum protein needs are influenced by the amount required for basic living,growth, and production. Cotton seed oilcake potential as a protein supplement foranimal feed because protein that present in cotton seed oilcake reaches 29.57 to31.13%. Cotton seed oilcake containing high protein and good quality given toruminants largely degraded by microbes in the rumen, so that influence to theefficiency of protein utilization. Protein is one of the important nutrients needed byruminants at various levels of production and the various phases of his life. A highprotein in feed ingredients needs to be done protection that are not easily degradedby rumen microbes Protection of proteins can be done in several ways, namelyphysical, chemical and biological. The study aims to assess fermentability feedingredients protein source that is protected by tannins from extracts of tea waste interms of measuring the concentration of NH3, Undegraded Protein and total proteinproduction. The results showed that the concentration of ammonia in cotton seedoilcake protected with increased of tannin levels decrease NH3 concentration andincrease the levels of UDP (p <0.05) and increase total protein. The average of NH3concentration, UDP and total protein in T0, T1, T2, T3 consecutive are (7,52 mM;10,91%; 369,62 mg/g), (6,60 mM; 11,45%; 439,50mg/g), (6,55 mM; 12,41%;457,35 mg/g), (6,32 mM; 13,44%; 514,95 mg/g). The conclusions of this study thatthe cotton seed oilcake protected dregs of tea tannin extracts with level 0,25%,0,50% and 0,75% can decrease the concentration of NH3, increase UDP and totalprotein. The protection level of Cotton seed oilcake with tannin extract the best teawaste is level 0,75% (% b/b BK).
PENAMBAHAN BIOAKTIVATOR PADA COMPLETE FEED DENGAN PAKAN BASAL RUMPUT GAJAH TERHADAP KECERNAAN BAHAN KERING DAN BAHAN ORGANIK SECARA IN VITRO Hadiyanto, Yuda Arief; Surono, Surono; Christiyanto, Marry
Animal Agriculture Journal Vol 1, No 1 (2012): Volume 1, Nomor 1, Tahun 2012
Publisher : Animal Agriculture Journal

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Abstract

Penelitian ini bertujuan untuk meningkatkan kualitas complete feed dengan penamb ahan bioaktivator terutama kecernaan bahan pakan. Materi dalam penelitian adalah complete feed. Penelitian dilakukan dalam 2 tahap yaitu tahap persiapan dan tahap analisis KcBK dan KcBO secara in vitro. Rancangan penelitian yang di gunakan adalah Rancangan Acak Lengkap dengan 5 perlakuan (T0, T1, T2, T3 T4) dan 4 ulangan. Hasil analisis ragam menunjukkan bahwa perlakuan penambahan bioaktivator mikroorganisme campuran pada complete feed berpengaruh terhadap KcBK dan KcBO secara in vitro. Rata-rata KcBK pada perlakuan T0, T1, T2, T3 dan T4 berturut-turut adalah 51,47; 51,92; 53,93; 48,11 dan 48,13%. Rata-rata KcBO 50,27; 49,51; 51,83; 44,53 dan 47,41%. Kesimpulan dari penelitian ini adalah penambahan bioaktivator mikrobia campuran dengan persentase cairan rumen sapi yang lebih banyak pada complete feed memberikan nilai KcBK dan KcBO yang lebih tinggi.Kata kunci : Bioaktivator, Complete Feed, Rumput gajahABSTRACT This study aims to improve the quality of complete feed mainly digestibility of feed ingredients. The material in this study is complete feed. The study was conducted in two stages, the preparatory phase and the analysis phase in vitro Dry Matter and Organic Matter Digestibility. The research design used was completely randomized design with 5 treatments and 4 replications. The results of the analysis showed that the treatment of a variety of microorganisms bioactivator addition to the complete feed mixture affect Digestibility Dry Matter and Organic Matter in vitro. Average Digestibility Dry Matters in treatment T0, T1, T2, T3 and T4, respectively, 51.47; 51.92; 53.93; 48.11 and 48.13%. Average Digestibility Organic Matter 50.27; 49.51; 51.83; 44.53 and 47.41%. The conclusion of this study is the addition of microbial bioactivator mixed with cow rumen fluid percentage more in the complete feed providing value Digestibility Dry Matter and Organic Matter higher.Keywords: bioactivator, Complete Feed,Napier grass
KECERNAAN BAHAN KERING DAN BAHAN ORGANIK SECARA IN VITRO HIJAUAN Desmodium cinereum PADA BERBAGAI DOSIS PUPUK ORGANIK CAIR DAN JARAK TANAM Setiyaningsih, Kristiana Desy; Christiyanto, Marry; Sutarno, Sutarno
Animal Agriculture Journal Vol 1, No 2 (2012): Volume 1, Nomor 2, Tahun 2012
Publisher : Animal Agriculture Journal

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Abstract

Penelitian dilakukan dengan tujuan untuk mengetahui dan mempelajari kecernaan bahan kering (KcBK) dan bahan organik (KcBO) secara in vitro hijauan D. cinereum pada berbagai dosis pupuk organik cair dan jarak tanam. Penelitian ini menggunakan rancangan acak lengkap (RAL) pola faktorial 4 x 2 dengan 3 ulangan. Dosis pupuk organik cair (P) sebagai faktor A (0, 1, 3, dan 5 ml/1 l air/petak) dan jarak tanam (S) sebagai faktor B (50 x 75 cm dan 25 x 50 cm). Parameter yang diamati adalah KcBK dan KcBO. Hasil penelitian menunjukkan bahwa KcBK dan KcBO yang tertinggi dicapai oleh P1S0 dibandingkan P2S0, P0S1, P2S1, P3S1, P1S1, P0S0 dan P3S0. Dosis pupuk organik cair (POC) berpengaruh nyata (p<0,05) terhadap KcBK dan KcBO. Jarak tanam tidak berpengaruh nyata terhadap KcBK dan KcBO. Nilai KcBK dan KcBO adalah 61,64; 61,39; 59,98; 59,67; 58,97; 58,86; 57,86; 53,88% dan 59,63; 59,45; 57,67; 57,23; 56,73; 56,15; 55,75; 48,02%. Dosis POC 3 ml/1 l air/petak (P2) memberikan pengaruh dengan nilai KcBK dan KcBO 60,53% dan 58,34% merupakan nilai kecernaan tertinggi.Kata kunci : D. cinereum; jarak tanam; pupuk organik cair; kecernaan in vitroABSTRACTThis research was conducted with the objective of identifying and studying the dry matter digestibility (DMD) and organic matter digestibility (OMD) in vitro forage D. cinereum at various doses of liquid organic fertilizer and plant spacing. This study used a completely randomized design (CRD) 4 x 2 factorial with three replications. Liquid organic fertilizer (P) as the factor A (0, 1, 3, and 5 ml / 1 l water / plot) and spacing (S) as the factor B (50 x 75 cm and 25 x 50 cm). Parameters measured were DMD and OMD. The results showed that the highest DMD and OMD achieved by comparison P1S0 P2S0, P0S1, P2S1, P3S1, P1S1, P0S0 and P3S0. Liquid organic fertilizer significantly (p <0.05) against DMD and OMD. Planting space did not significantly affect DMD and OMD. DMD and OMD value is 61.64; 61.39; 59.98; 59.67; 58.97; 58.86; 57.86; 53.88% and 59.63; 59.45; 57.67; 57.23; 56.73; 56.15; 55.75; 48.02%. Liquid organic fertilizer dose 3 ml / 1 l water / plot (P2) to give effect to the values DMD 60.53% and OMD 58.34% which is the highest digestibility values.Keyword : D. cinereum; planting space; liquid organic fertilizer; in vitro digestibility
PENGARUH PROTEKSI PROTEIN AMPAS KECAP DENGAN TANIN TERHADAP KONSENTRASI AMONIA, PRODUKSI PROTEIN TOTAL DAN PERSENTASE RUMEN UNDEGRADED DIETARY PROTEIN SECARA IN VITRO Mayangsari, Nadia Sitha; Subrata, Agung; Christiyanto, Marry
Animal Agriculture Journal Vol 2, No 1 (2013): Volume 2, Nomor 1, Tahun 2013
Publisher : Animal Agriculture Journal

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Abstract

ABSTRAK Penelitian bertujuan untuk mengetahui pengaruh level penambahan tanin daun mangrove untuk memproteksi protein ampas kecap terhadap konsentrasi amonia (NH3), produksi protein total dan persentase rumen undegraded dietary protein (RUDP) secara in vitro. Materi yang digunakan adalah ampas kecap, daun mangrove sebagai sumber tanin alami, cairan rumen dan reagensia yang digunakan dalam analisis NH3, protein total dan RUDP. Parameter yang diamati adalah konsentrasi NH3, produksi protein total dan persentase RUDP. Penelitian dilaksanakan menggunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 4 ulangan. Perlakuan adalah T0 (ampas kecap + tanpa tanin), T1 (ampas kecap + tanin 0,25%), T2 (ampas kecap + tanin 0,50%), T3 (ampas kecap + tanin 0,75%). Hasil penelitian diolah secara statistik dengan analisis sidik ragam dan jika terdapat pengaruh nyata akibat perlakuan dilanjutkan dengan uji Duncan dengan taraf 5% untuk mengetahui adanya perbedaan pengaruh antar perlakuan. Hasil penelitian didapatkan konsentrasi NH3 protein ampas kecap terproteksi dengan perbedaan aras tanin berturut-turut adalah 4,34; 3,46; 3,29; 3,10 mM. Produksi protein total yang dihasilkan berturut-turut adalah 315,37; 330,05; 334,04; 304,45 mg/g. Persentase RUDP yang dihasilkan berturut-turut adalah 27,91; 27,96; 28,03; 27,36 %. Hasil uji variansi menunjukkan bahwa proteksi protein ampas kecap menggunakan tanin mangrove berpengaruh terhadap konsentrasi NH3 namun tidak berpengaruh terhadap produksi protein total dan persentase RUDP. Hasil uji lanjut DMRT menunjukkan bahwa konsentrasi NH3 pada perlakuan T0 nyata lebih tinggi (p<0,05) dari T1, T2 dan T3 sedangkan T1, T2 dan T3 tidak berbeda nyata (p>0,05). Dari hasil penelitian dapat disimpulkan bahwa proteksi protein ampas kecap menggunakan tanin daun mangrove terbukti menurunkan konsentrasi amonia (NH3) tetapi tidak mempengaruhi produksi protein total dan persentase RUDPnya.Kata kunci: ampas kecap; tanin; amonia; protein totalABSTRACTThe aimed of this research was to determine the effect of level tannin of mangrove leaf to protected soy pulp protein on ammonia concentration (NH3), total protein production and percentage of rumen undegraded dietary protein (RUDP) in vitro. The material used is soy pulp, mangroves leaves as a natural source of tannins, rumen liquor and reagents used in the analysis of NH3, total protein and RUDP. Parameters measured were NH3 concentrations, total protein production and percentage of RUDP. The experiment was conducted used a completely randomized designed with 4 treatments and 4 replications. Treatments are T0 (soy pulp + without tannin), T1 (soy pulp + tannin 0.25%), T2 (soy pulp + tannin 0.50%), T3 (soy pulp + tannin 0.75%). The results were analyzed statistically by variance analysis, and if there is a real effect due to treatment followed by Duncan test at 5% level to detect a difference between treatment effects. The results obtained NH3 concentration of soy pulp protein protected with different tannin are 4.34; 3.46; 3.29; 3.10 mM. The results total protein production are 315.37; 330.05; 334.04; 304.45 mg/g. The results percentage RUDP are 27.91; 27.96; 28.03; 27.36%. The variant test results showed that the protective soy pulp protein used mangrove tannin significantly decrease NH3 concentrations, but no significantly on total protein production and percentage RUDP. The DMRT test result showed that NH3 concentrations on T0 significantly higher (p<0.05) from T1, T2 and T3 while T1, T2 and T3 not significantly different (p>0.05). From the results of this research concluded that the protection of soy pulp protein used mangrove leaves tannin proven to decrease ammonia concentration (NH3) but did not affected to total protein production and percentage RUDP.Keywords: soy pulp; tannin; ammonia; total protein