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All Journal ACTA VETERINARIA INDONESIANA Bioscience AL KAUNIYAH BERITA BIOLOGI Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Journal of Aquaculture and Fish Health Bioscientist : Jurnal Ilmiah Biologi Jurnal Pijar MIPA Ahmad Dahlan Medical Journal Gunung Djati Conference Series Jurnal Kedokteran Hewan Jurnal Ekonomi, Manajemen, Akuntansi
Haris Setiawan
Departement Of Biology, Faculty Of Applied Science And Technology, Universitas Ahmad Dahlan, Jl. Kapas No. 9, Yogyakarta, Indonesia, 55166
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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ANTISPERMATOGENIC ACTIVITY OF ETHANOLIC EXTRACT OF CALINA PAPAYA LEAVES ON SEMINIFEROUS TUBULES WISTAR RATS Haris Setiawan; Sri Wijayanti Wulandari; Erlita Devi Agustina
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (659.838 KB) | DOI: 10.21157/j.ked.hewan.v15i1.18435

Abstract

Indonesia is a country with high birth rate and the rate continues to increase each year. Methods to reduce high birth rate are needed, especially herbal based contraceptives. This research aims to study the possibility of Calina papaya leaf ethanolic extract as an antispermatogenic agent which hopefully could be expanded into natural antifertiliy drug candidate. Research was done using 24 white male Wistar rats divided into four groups. Dosages were given as follows: 0 mg/Kg W (control group), 100 mg/Kg W (P1), 200 mg/Kg W (P2) and 300 mg/Kg W (P3). Treatment was given in 30 days period. Rats were sacrificed on Day 31 and the testes were taken. Testes were then made into histological specimen using paraffin block and stained using Haematoxylin-Eosin. The diameter and surface area of seminiferous tubules, lumen surface area, and spermatogenesis index were then observed. Results showed that there is a significant difference (p0.05) on lumen surface area and spermatogenesis index between control and treatment groups. It is also found that the optimum dosage given to attain the desirable effect is 300 mg/Kg W. There is no significant difference found on surface area and diameter of tubulus seminiferous between control and treatment group (p0.05). Based on the results, papaya leaf (Calina variety) ethanolic extract has the potential a natural antispermatogenic with the optimum dose of 300 mg/Kg W.
Morfologi Insang Ikan Lele Mutiara (Clarias gariepinus Burchell, 1822) yang Diberi Paparan Mikroplastik Polietilen (PE) pada Pakan Nurul Suwartiningsih; Glady Sunggoro; Raffly Muhammad Dhiaulhaq; Lyly Nur Indah Sari; Kurnia Suci Maharani; Ichsan Luqmana Indra Putra; Haris Setiawan
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7702

Abstract

Microplastics in the body of fish can damage the function of organs. One of the aquatic biota that can be affected by microplastics is Mutiara catfish. The study aimed to compare the gill morphology of Mutiara catfish after being exposed to microplastics.  This study was an experimental study consisting of four treatments, feed treatment with microplastic added with a weight of 0.00 mg/0.75 g feed (control); 0.01 mg/ 0.75 g feed (P1); 0.1 mg/0.75 g (P2); and 1 mg/0.75 g (P3). Each treatment was repeated seven times (seven buckets) and each bucket was filled with five fish so that a total of 140 fish were used. The treatment was carried out for 28 days. Observation of growth parameters was carried out on the entire population, 140 fish, while organ preparation was carried out on a sample of three fish from each treatment. Fish samples were taken using a stratified random sampling technique. Research parameters include gill weight, primary lamela length (LP) and secondary lamela lenght (LS). The results showed the highest gill weight at control 1.76 ± 0.47 g and the lowest at P1 1.20 ± 0.25 g, although it did not significantly different. The highest gill weight/ body weight at control 0.06 ± 0.02 g and the lowest P1 and P2 0.04 ± 0.00 g, although it did not significantly different. Primary lamela length (LP) was highest at P3 1250.34 ± 294.81 μm and lowest at Control 894.78 ± 399.10 μm, and it was significantly different. Secondary lamela lenght (LS) was highest at P3 33.69 ± 4.34 μm and lowest at P1 29.12 ± 5.29 μm. Among controls, P2 and P3; LS length did not significantly different. Microplastic PE up to 1.00 mg/ 0.75 g of feed does not result in a decrease of gill weight and elongation of secondary lamela, but results in significant lengthening of primary lamela.
The Effects of Calina Papaya Leaf Ethanol Extract On Estrus Cycle And Uterus Morphology of Wistar Rats Haris Setiawan; Sri Wijayanti Wulandari; Fauziana Eka Nurwidyantary; Intan Dewantari
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 3 (2021): December 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i3.31343

Abstract

Calina papaya leaf is an Indonesian papaya plant type that has the potential to be used as a medicinal plant. Phytochemical compounds found in papaya leaves act as antifertility agents. The research aimed to study the antifertility effect of Calina papaya leaf ethanol extract on estrus cycle and uterine morphology of wistar rats. The experiment lasted 30 days and involved 25 female wistar rats. The treatment consisted of 5 groups, namely K (distilled water), K+ (contraceptive pill), P1 (Calina papaya leaf ethanol extract 200mg/KgBW), P2 (Calina papaya leaf ethanol extract 300mg/KgBW), and P3 (Calina papaya leaf ethanol extract 400mg/KgBW). The parameters of estrus cycle consisted of estrus cycle phase, total cycle and estrus cycle ratio. Uterus morphological parameters consist of wall thickness of the endometrium, myometrium, and perimetrium. The estrus cycle examination was carried out every day using the vaginal smear method (Giemsa 5%). On the 31st day, surgery was performed and the uterus was taken for histological observation using the paraffin method (HE staining). Data were analyzed using One Way ANOVA with further Duncan’s test. The results showed that a dose of 400 mg/KgBW could affect the estrus cycle with accelerate the estrus phase and increase the estrus cycle ratio compared to the control (P0.05). There was a decrease in endometrium and myometrium wall thickness at a dose of 400 mg/KgBW compared to the control (P0.05). The conclusion of the study is that the ethanol extract of Calina papaya leaves at a dose of 400 mg/KgBW has the potential to be a natural antifertility agent on female wistar rats.
Pengaruh Pemberian Jus Pare (Momordica charantia L.) Terhadap Kualitas Sperma dan Histologi Testis Tikus Wistar Haris Setiawan; Roby Ahmad Subagja
Al-Kauniyah: Jurnal Biologi Vol 17, No 1 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.1.23216

Abstract

 AbstrakBuah pare merupakan salah satu kandidat agen kontrasepsi karena memiliki beberapa senyawa seperti flavonoid, triterpenoid, steroid, charantin, dan momordicin yang memiliki peran sebagai agen antispermatogenik. Penelitian bertujuan untuk mengetahui pengaruh pemberian jus pare (Momordica charantia L.) terhadap kualitas sperma dan histologi testis tikus Wistar (Rattus norvegicus Berkenhout, 1769). Penelitian menggunakan 20 ekor tikus Wistar jantan, dibagi menjadi 4 perlakuan yang terdiri dari kelompok pemberian akuades (K), kelompok pemberian jus pare konsentrasi 25% (P1), 50% (P2), dan 75% (P3) yang dilakukan selama 49 hari menggunakan sonde lambung 1 mL. Pada hari ke-50 tikus dibedah untuk diambil cauda epididymis dan testis. Cauda epididymis dilarutkan ke dalam Phospate Buffer Saline (PBS) untuk pengamatan kualitas sperma yang terdiri dari motilitas, jumlah, viabilitas, dan morfologi sperma. Testis dibuat sediaan histologi dengan metode parafin (pewarnaan Hematoxylin-Eosin). Seluruh parameter dianalisis menggunakan uji One-Way Anova dan dilanjutkan Duncan test (P <0,05). Hasil menunjukkan terdapat penurunan motilitas, viabilitas, dan jumlah sperma pada konsentrasi 50% dibandingkan dengan kontrol (P <0,05), namun tidak terdapat perbedaan pada morfologi sperma (P >0,05). Terdapat penurunan jumlah sel spermatogonium, spermatosit, dan spermatozoa pada konsentrasi 25% dibandikan dengan kontrol (P <0,05), namun tidak terlihat penurunan pada sel spermatid dan index spermatogenesis (P >0,05). Jus pare dapat menurunkan sebagian besar parameter kualitas sperma sehingga berpotensi sebagai antispermatogenik.AbstractBitter melon fruit is one of the candidates for contraceptive agents because it contains several compounds such as flavonoids, triterpenoids, steroids, charantin, and momordicin which have a role as antispermatogenic agents. The research aims to determine the effect of giving bitter melon juice (Momordica charantia L.) on sperm quality and testicular histology of Wistar rats (Rattus norvegicus Berkenhout, 1769). The study used 20 male Wistar rats, divided into 4 treatments consisting of groups given distilled water (K), groups given bitter melon juice with concentrations of 25% (P1), 50% (P2), and 75% (P3) which were carried out for 49 days. using a 1 mL gastric probe. On the 50th day, mice were dissected to remove the cauda epididymis and testes. The cauda epididymis is dissolved in Phosphate Buffer Saline (PBS) to measure sperm quality consisting of sperm motility, number, viability and morphology. Testes were made into histological preparations using the paraffin method (Hematoxylin-Eosin staining). All parameters were analyzed using the One-Way Anova test and continued with the Duncan test (P<0.05). The results showed that there was a decrease in sperm motility, viability and number at a concentration of 50% compared to the control (P<0.05), but there was no difference in sperm morphology (P>0.05). There was a decrease in the number of spermatogonium cells, spermatocytes and spermatozoa at a concentration of 25% compared to the control (P<0.05), but there was no visible decrease in spermatid cells and spermatogenesis index (P>0.05). Bitter melon juice can reduce most sperm quality parameters so it has the potential to be antispermatogenic. 
Kaidah Hukum Ekonomi Syariah dalam Transaksi Miras Risky Willy Dama; Ahmad Untung; Haris Setiawan
EKOMA : Jurnal Ekonomi, Manajemen, Akuntansi Vol. 3 No. 4: Mei 2024
Publisher : CV. Ulil Albab Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56799/ekoma.v3i4.3789

Abstract

Penelitian ini bertujuan untuk melihat bagaimana Kaidah Hukum Ekonomi Syariah Dalam Transaksi Miras Proses fermentasi tersebut juga mengakibatkan terbentuknya kadar alkohol (etanol), sehingga terlarang dan bertentangan dengan syari’at Islam karena termasuk bagian dari khamar. Penelitian ini bersifat deskriptif kualitatif, Minuman keras adalah minuman yang mengandung alkohol yang bila dikonsumsi secara berlebihan dan terus-menerus dapat merugikan dan membahayakan jasmani, rohani maupun bagi kepentingan kejiwaan sehingga akibat lebih lanjut akan mempengaruhi kehidupan keluarga dan hubungan dengan masyarakat sekitar. Masalah miras ini seperti hal yang sudah tidak asing lagi di kalangan masyarakat Maluku Utara. Ada beberapa yang menjadi munuman tradisonal dalam tradisi adat di Maluku Utara seperti air nira yang keluar dari pohon aren (pinnata) atau yang disebut masyarakat dengan pohon seoh yang di fermetasi manjadi minuman cap tikus yang dapat memabukan. Yang terjadi seperti cap tikus siap edar di Ternate dengan nama yang berbeda yaitu minuman akar lagi viral-viralnya di Ternate cap tikus yang di rendam dengan akar pohon sehingga minuman tersebut berwana menjadi seperti warna teh. Dalam hal transaksi minuman captikus yang di edarkan tersebut sudah di larang dari pihak yang berwanang namun masyarakat setempat masih saja mengkonsumsi minuman tersebut yang memang benar-benar tidak kapok dengan larang. Hal ini di karenakan kurangnya pemahaman agam yang begitu lemah di kalangan masyarakat tersebut karna merkan juga mengetahui apa yang di pelakukan tersebut ialah haram sesuai dengan syariat islam dalam al-qur’an dan hadist.
Co-Authors Ahmad Untung Akhmad Nizzar Nasikhudin Anisa Fatwa Ardaning Nuriliani Aritasya Nur Fitriyani Arlinda Sekar Ayu Hikmawati Elgio Venanda Ginting Eriza Via Malinda Erlita Devi Agustina Fauziana Eka Nurwidyantary Febriofca Galih Yatalaththov Glady Sunggoro Hendry Tri Sakti Saragih Hendry Trisakti Saragih Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Intan Dewantari Kurnia Suci Maharani Listiatie Budi Utami Listiatie Budi Utami Luthfiana Ulil Albab Lyly Nur Indah Sari Maliza, Rita Manesta Edelweis Jingga Muhammad Ilham Hisbullah Muhammad Zulfikar Novi Febrianti Nur Rifka Istiqomah Pijar Ridho Prihandana Raffly Muhammad Dhiaulhaq Rahmat Krestianto Ridwan Alfatah Risky Willy Dama Roby Ahmad Subagja Sri Wijayanti Wulandari Sri Wijayanti Wulandari Sri Wijayanti Wulandari Sri Wijayanti Wulandari Sri Wijayanti Wulandari Sri Wijayanti Wulandari Stephanus Ardi Dimar Suci Resty Rahmadini Suwartiningsih, Nurul Syaiful Adam Maulana Syalimah Yani Aruan