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All Journal Majalah Kedokteran Bandung Althea Medical Journal Sari Pediatri Jurnal Sistem Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Nadjwa Zamalek Dalimoenthe
Departemen Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Umum Pusat Dr. Hasan Sadikin, Bandung
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health Social Sciences

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HASIL HITUNG NORMOBLAS ANTARA SEDIAAN HAPUSAN DARAH TEPI PENDERITA AML DENGAN ALL Hidayat .; Nina Susana Dewi; Nadjwa Zamalek Dalimoenthe
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.997

Abstract

Normoblast is an immature form of erythrocyte in erythropoietin system. Normally, normoblast can be found in peripheral blood healthy neonates. The existence of normoblast in peripheral blood might be the sign of pathologic conditions such as hemolytic anemia,acute blood loss, and ischemia and bone marrows abnormalities like malignancy or leukemia. In acute leukemia (Acute MyeloblasticLeukemia and Acute Lymphoblastic Leukemia), normoblast existence in peripheral blood may due to erythropoietin system suppression.The aim of this study is to compare normoblast count between AML and ALL, and also to find out the correlation between leukocyte andnormoblast count in AML and ALL. The subject of this study were patient diagnosed as AML (30) and ALL (30) in Hematology Divisionof Clinical Pathology Department at Dr.Hasan Sadikin Hospital Bandung in July 2006–August 2008. In this study we examined 30peripheral blood smears from AML and 30 peripheral blood smears from ALL. Leukocyte count result was derived from CBC performedwith Sysmex KX-21. The mean value of normoblast count from AML blood smear patients is 1930.60 (3.60/100 WBC) while ALL bloodsmear patients is 309.60 (0.43/100 WBC). Statistically this difference is significant (p < 0.001). There are strong correlation betweenleukocyte count and normoblast count within both group (r = 0.851, r = 0.948; p < 0.001).
LEUKOSITOSIS BER-FLAGGING BINTANG () BERPOTENSI ADANYA INTERFERENSI ALAT ANALISIS HEMATOLOGI OTOMATIS Christine Sugiarto; Leni Lismayanti; Nadjwa Zamalek Dalimoenthe
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1026

Abstract

Leukocytosis is a condition in which there is an increasing number of white blood cell count in the peripheral blood compared to thenormal range based on age. Several conditions can amplify leukocyte count from haematological auto analyzers, not only those whichcorrespond to the pathologic and physiologic condition, but also with other factors, such as diluent and haematological auto analyzer’smethods. The information about these factors should be evaluated to lessen errors in the patient’s diagnosis and therapy. This casereport describes a leukocytosis in a 35-day old baby boy, diagnosed as duodenal obstruction, admitted in Paediatric Surgery Department,Hasan Sadikin Hospital, Bandung. Discrepancies occurred in this patient’s leukocyte count with some different haematological autoanalyzers. The leukocyte count from the auto analyzer by impedance method and ammonium salt diluent was 129.200/mm3 which wasindicated by a star-flagged (), while from the auto analyzer with light scatter method and anhydrous sodium sulphate and sodiumchloride diluent was 9.200/mm3, from manual count by the counting chamber with Turk diluent was 14.200/mm3 and the estimationby peripheral blood smear was 7.000–10.000/mm3. False leukocytosis by auto analyzer with impedance method was caused by thelimitation of the analyzer’s method and by the erythrocyte lysine reagent (diluent) using ammonium salt. As investigated in this case,the interferences were thought as being caused by the Lyses-resistant Red Blood Cells, thus the non-lysed/lyses cells which were enlargedin size were identified as leukocytes other than erythrocytes. It can be that the white blood concluded cell count examination which isindicated by star-flagged (), or white blood cell count >100.000/mm3 must be confirmed by manual examination (counting chamberand peripheral blood smear) or by another haematological auto analyzer method that has a different and more potent diluent
THE CORRELATION BETWEEN THE MEAN PLATELET VOLUME VALUES WITH THROMBOCYTE AGGREGATION IN NEPHROPATHY DIABETIC PATIENTS Agus Sunardi; Nadjwa Zamalek Dalimoenthe; Coriejati Rita; Adhi Kristianto Sugianli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 1 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i1.1510

Abstract

     Diabetic nephropathy is the most important cause of end-stage renal failure. Chronic hyperglycemia will cause glomerular endothelial damage, and this damage will stimulate hemostasis activation including platelets so that platelet aggregation will increase. The increase of platelet aggregation will increase platelet consumption, which further stimulates thrombopoiesis which will lead to immature platelets of large size to be released into the circulation. This research aimed to determine the positive correlation between MPV with platelet aggregation in patients with diabetic nephropathy. This study was an analytic observational study with a cross-sectional study design. The research was conducted in the Dr. Hasan Sadikin Hospital Bandung from July 2016 to October 2017. A total of 52 subjects who met the inclusion criteria were included in the study. Mean platelet volume and platelet aggregation were performed with venous examination with EDTA and sodium citrate 3.2% anticoagulants. The result of platelet aggregation examination showing platelet hyper-aggregation was found in 44.2% of subjects, 50% normal-aggregation, 5.8% hypo-aggregation. While the median value of MPV in this study was 9.2 fL with the range of 8.00 – 11.80 fL. A positive correlation was found  between MPV value with platelet aggregation with r= 0.067, p= 0.634. The conclusion was that there was no correlation between MPV values with platelet aggregation in diabetic nephropathy patients. This small and insignificant r-value might be due to several factors that also affect platelet aggregation in diabetic nephropathy patients, requiring further investigation.
PROPORTION OF RHESUS BLOOD TYPE PHENOTYPES OF ROUTINE BLOOD DONORS AT BLOOD DONOR UNIT INDONESIAN RED CROSS IN BANDUNG CITY Ivana Dewi; Nadjwa Zamalek Dalimoenthe; Anna Tjandrawati; Nida Suraya
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.807

Abstract

Blood type phenotype incompatibility including Rhesus between donors and patients may result in cross-matching problems, triggering alloimmunization, and caused Hemolytic Transfusion Reaction (HTR). Rhesus blood type incompatibility between mother and fetus may cause Hemolytic Disease of Fetus and Newborn (HDFN). Pretransfusion phenotypic blood groups matching also reduce cost efficiently which means antibody screening is no longer needed. The purpose of the study is to find the proportion Rhesus blood type phenotypes in routine blood donors at Blood Donor Unit, Indonesian Red Cross in Bandung city, Indonesia.The study is a descriptive and observational with cross sectional design. The study was done at Blood Donor Unit, Indonesian Red Cross in Bandung city from April 2016 to September 2017. The subjects were 142 routine blood donors, blood sampling was done simultaneously with blood donation. Rhesus antigen examination of ethylenediaminetetraacetic acid (EDTA) blood was done by gel method. Subjects characteristics were male (68%) and female (32%), with the mean age was 39 years. Examination of Rhesus antigen were found antigen D(100%), antigen e(98,6%), antigen C(97,9%), antigen c(38,7%) and antigen E(31,7%). Results of Rhesus phenotypes were DCe/DCe(61,3%), DCe/DcE(29,6%), DCe/dce(7%), DcE/DcE(1,4%) and DcE/dce(0.7%). The distribution of Rhesus blood type were affected by factors such as genetics, race, ethnicity, marriage, demography, and migration. Rhesus blood phenotypes proportion in routine blood donors at Blood Donor Unit, Indonesian Red Cross in Bandung city sorted from the most are DCe/DCe, DCe/DcE, DCe/dce, DcE/DcE and DcE/dce.Keywords: blood donor, phenotype, Rhesus
Co-Authors A.A. Ketut Agung Cahyawan W Adhi Kristianto Sugianli Agus Sunardi Anna Tjandrawati Bryant Alyzandyr Eumhyn Chua Bryant Alyzandyr Eumhyn Chua, Bryant Alyzandyr Eumhyn Christine Sugiarto Coriejati Rita Dewi Kartika Turbawaty Emmy Pranggono Fenny, - Heny Syahrini Hidayat . Ivana Dewi Leni Lismayanti Loekman Prawirakoesoema Marizka Adzani Marizka Adzani, Marizka Maula Rifada Mohammad Ghozali Mohammad Ghozali, Mohammad Nida Suraya Nina Susana Dewi Noormartany putri alyumnah putri alyumnah, putri Rony, Zahara Tussoleha Sutarya Enus Tena Rosmiati Iskandar Tetty Yuniaty Trinugroho Heri Fadjari Trully D. Sitorus Trully D. Sitorus