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Noormartany
Universitas Islam Bandung
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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Korelasi Jumlah CD4 Dan Total Lymphocyte Count (Tlc) pada Penderita HIV/AIDS dengan dan tanpa Terapi Antiretroviral Sulianto, Ivana Agnes; Indrati, Agnes R; Wisaksana, Rudi; Noormartany, Noormartany
Global Medical & Health Communication (GMHC) Vol 1, No 1 (2013)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

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Abstract

ABSTRAK Jumlah CD4 merupakan parameter laboratorium yang digunakan untuk memulai dan memantau terapi antiretroviral (ART) pada penderita HIV/AIDS. Namun pemeriksaan jumlah CD4 membutuhkan peralatan laboratorium yang mahal dan tenaga terlatih. World Health Organization (WHO) merekomendasikan total lymphocyte count (TLC) sebagai pengganti CD4 dalam memulai terapi. Penelitian ini bertujuan untuk melihat korelasi antara jumlah CD4 dan TLC pada data dasar, pemantauan pertama dan kedua penderita HIV/AIDS sebagai dasar digunakannya TLC untuk pemantauan terapi. Penelitian ini merupakan penelitian observasional analitik dan bagian dari penelitian kohort IMPACT (Integrated Management for Prevention And Care and Treatment of  HIV/AIDS) pada pasien HIV/AIDS di RS. Dr. Hasan Sadikin Bandung. Data tersebut dibagi menjadi kelompok tanpa ART dan dengan ART, masing-masing kelompok dibagi berdasarkan jenis kelamin. Analisis korelasi dilakukan pada data CD4 dan TLC dari tiap kelompok.Penelitian ini menggunakan 2239 data. Korelasi antara CD4 dan TLC pada data dasar pria tanpa ART adalah 0.644 (p=0.01), wanita tanpa ART adalah 0.74 (p=0.01), pria dengan ART 0.67 adalah (p=0.01), wanita dengan ART adalah adalah 0.601 (p=0.01). Korelasi antara CD4 dan TLC pemantauan pertama pria tanpa ART 0.56 (p=0.01), wanita tanpa ART adalah 0.606 (p=0.01), pria dengan ART adalah 0.569 (p=0.01), wanita dengan ART adalah 0.466 (p=0.01). Korelasi antara CD4 dan TLC pemantauan kedua pria tanpa ART adalah 0.697 (p=0.01), wanita tanpa ART adalah 0.306 (p=0.01), pria dengan ART adalah 0.556 (p=0.01), wanita dengan ART adalah 0.561 (p=0.01).  Kesimpulan :  terdapat korelasi yang baik antara jumlah CD4 dan TLC, sehingga TLC dapat digunakan sebagai alternatif pemantauan terapi sebelum penderita melakukan pemeriksaan CD4.   Kata kunci: CD4, HIV/AIDS, terapi antiretroviral, total lymphocyte count
The correlation between serum neuron-specific enolase level in adult patients of oromaxillofacial fracture with mild head injury Ruslin, M.; Yusup, HY; Arifin, MZ; Noormartany, N.
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

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Abstract

Oromaxilofacial fracture will have associated with head injury due to its adjacent location and structure. The oromaxillofacial structure is considered to be as force silencer for trauma so that it can protect the intracranial structures. Neuron-specific enolase (NSE) is a specific enzyme within neuron cells and can be detected rapidly in serum. The objective of the study was to investigate the increased serum NSE level in adult patients with oromaxillofacial fracture with mild head injury; and to investigate the correlation between serum NSE level and the location of oromaxillofacial fracture (upper, middle, lower regions, or the combinations) in adult patients with mild head injury. This study used the analytical observational method with a cross-sectional design, that conducted in Department of Neurosurgery, Department of Oral and Maxillofacial Surgery, and Laboratory of Clinical Pathology, Dr Hasan Sadikin Hospital, Bandung, from August to October 2008. The serum NSE level examination was undertaken by blood sample taking then examined with electrochemiluminescence immunoassay (ECLIA). The data were analyzed using Mann-Whitney test to seek the increased serum NSE level; using Kruskal-Wallis to seek the mean of increased serum NSE levels by location of oromaxillofacial fracture; and using Spearman ranked correlation test to seek the correlation between the increased serum NSE levels and location of oromaxillofacial fracture in adult patients with mild head injury. The study results indicated that there was an increased serum NSE level in adult patients with oromaxillofacial fracture with mild head injury by 13.12 ng/mL compared to adult healthy group by 7.72 ng/mL (p<0.001). By upper, middle, and lower regions of oromaxillofacial fracture, the serum NSE level of oromaxillofacial fracture at combined upper-middle-lower region was significantly the highest, i.e., 21.14 ng/mL (p=0.049). A positive correlation between the increased serum NSE level and location of oromaxillofacial fracture caused by mild head injury existed (rs=0.33, p=0.020). In conclusion, there was an increased serum NSE level in adult patients with oromaxillofacial fracture with mild head injury; and there was also a positive correlation between the increased serum NSE level and location of oromaxillofacial fracture due to mild head injury.
Kadar Asam Urat Serum sebagai Biomarker Preeklamsi Sumanti, Neli; Noormartany, -; Alamsyah, Muhammad; Rostini, Tiene
Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Preeklamsi masih merupakan masalah kesehatan yang menjadi salah satu penyebab kematian ibu selain akibat perdarahan dan infeksi. Etiologi dan patogenesis preeklamsi belum diketahui pasti. Peningkatan kadar asam urat serum timbul bersamaan dengan peningkatan tekanan darah dan terjadi sebelum timbul proteinuria sehingga dapat dipakai sebagai salah satu biomarker. Tujuan penelitian ini menganalisis perbandingan kadar asam urat serum pada kehamilan in partu normal dengan in partu preeklamsi. Penelitian potong lintang dilakukan di Rumah Sakit Dr. Hasan Sadikin Bandung pada bulan Maret–Mei 2011. Subjek penelitian 45 wanita hamil in partu normal sebagai kelompok kontrol dan 44 wanita in partu dengan preeklamsi sesuai dengan kriteria inklusi dan eksklusi. Kadar asam urat serum pada wanita hamil normal adalah 3,43±0,14 mg/dL. Pada penelitian ini nilai titik potong kadar asam urat serum 4,8 mg/dL dengan nilai sensitivitas 93% dan spesifisitas 80%. Simpulan,kadar asam urat pada in partu preeklamsi lebih tinggi dibandingkan dengan kehamilan normal. Peningkatan kadar asam urat dapat dipertimbangkan sebagai salah satu biomarker preeklamsi, sebaiknya kadar asam urat serum digunakan sebagai pemeriksaan serial pada ibu hamil saat pelayanan antenatal. [MKB. 2013;45(2):98–104]Kata kunci: Biomarker, kadar asam urat, preeklamsiSerum Levels of Uric Acid as a Biomarker in PreeclampsiaPreeclampsia remains a health problem that becomes one of the causes of maternal deaths besides bleeding and infection. The etiology and pathogenesis of preeclampsia are unclear. Increased serum uric acid levels is seen simultaneously with the increase of blood pressure and occurred before the onset of proteinuria. Therefore, the uric acid can be used as a biomarker. The aim of this study was to analyze the serum uric acid levels between normal and preeclampsia pregnancies. The study was conducted in Dr.Hasan Sadikin Hospital Bandung between March and May 2011, using cross sectional study design. Subjects were 45 inpartu normal pregnant women as control and 44 in partu pregnant women with preeclampsia accordance with inclusion and exclusion criteria. Levels of uric acid in normal pregnancy are 3,43 ±0.14 mg/dL. In this study uric acid levels resulting in cut-off levels of 4,8 mg/dL with a sensitivity value of 93%, and specificity 80%. Conclusions: uric acid levels in at term preeclampsia are higher compared with normal pregnancies. Increased levels of uric acid can be considered as one of biomarkers of preeclampsia, hence the serum uric acid levels used as serial examinations in pregnant women during antenatal care. [MKB. 2013;45(2):98–104]Key words: Biomarker, preeclampsia, uric acid level DOI: http://dx.doi.org/10.15395/mkb.v45n2.89
Korelasi Proteinuria Metode Rasio Albumin-Kreatinin Urin dengan Metode Kromatografi pada Preeklamsi Syuhada, -; Noormartany, -; Alamsyah, Muhammad; Dewi, Nina Susanna
Majalah Kedokteran Bandung Vol 44, No 4 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Preeklamsi masih merupakan salah satu penyebab utama kematian ibu melahirkan. Pada preeklamsi terjadi kerusakan endotel vaskular ginjal yang menyebabkan proteinuria. Pemeriksaan proteinuria penting dalam diagnosis preeklamsi. Metode rasio albumin-kreatinin urin (uACR) adalah metode pemeriksaan proteinuria yang memiliki korelasi yang baik dengan kadar protein urin 24 jam sebagai baku emas. Metode lain adalah metode kromatografi (carik celup) yang masih secara luas digunakan di berbagai fasilitas kesehatan namun dari berbagai penelitian metode ini memiliki variasi nilai sensitivitas dan spesifisitas yang luas. Tujuan penelitian untuk mengetahui apakah terdapat korelasi yang baik antara hasil pemeriksaan proteinuria metode carik celup dan metode uACR pada penderita preeklamsi. Penelitian dilakukan di Rumah Sakit Dr. Hasan Sadikin Bandung. Subjek penelitian adalah wanita hamil penderita preeklamsi yang sesuai dengan kriteria inklusi dan tidak masuk ke dalam kriteria eksklusi. Analisis statistik dilakukan dengan uji korelasi rank Spearman. Subjek penelitian sebanyak 52 orang. Kelompok usia terbanyak adalah 20–34 tahun. Dari uji statistik yang dilakukan, didapatkan korelasi yang baik antara hasil pemeriksaan proteinuria dari kedua metode tersebut (r=0,51; p<0,05). Simpulan, terdapat korelasi yang baik antara hasil pemeriksaan proteinuria metode carik celup dan metode uACR pada penderita preeklamsi, sehingga metode carik celup dapat tetap dipakai. [MKB. 2012;44(4):218–23].Correlation between Urinary Albumin Creatinin Ratio Test and Chromatographic Method in PreeclampsiaPreeclampsia is still one of the major causes of maternal mortality. In preeclamptic renal vascular endothelial damage occurs that leads to proteinuria. Examination of proteinuria is essential in preeclampsia diagnosis. Urinary albumin-creatinine ratio (uACR) is one of proteinuria test methods with good correlation with 24-hour urinary protein as the gold standard. Another method is chromatographic method (dipstick urinalysis) which is still widely used in various health facilities but from various studies it has wide variation of sensitivity and specificity values. The purpose of this study was to determine whether there is a good correlation between the results of the proteinuria examination by dipstick method and uACR method in patients with preeclampsia. The study was conducted at Dr. Hasan Sadikin Hospital Bandung. Subjects were woman with preeclampsia in accordance with inclusion and exclusion criteria. Statistical analysis was performed using Spearman rank correlation test. There were 52 subjects. Thelargest age group was 20–34 years. From the statistical test performed, agood correlation between the results of proteinuria examination from both methods was found. In conclusion, there is a good correlation between the results of dipstick urinalysis method and the method of urinary albumin-creatinine ratio in patients with preeclampsia. Therefore, dipstick urinalysis examination can still be utilized. [MKB. 2012;44(4):218–23]. DOI: http://dx.doi.org/10.15395/mkb.v44n4.139
Kadar N-Terminal Pro-Brain Natriuretic Peptide sebagai Prediktor Luaran Klinis Sindrom Koroner Akut Tandhana, Florencia Idajanti; Noormartany, Noormartany; Aprami, Toni M.; Tristina, Nina
Majalah Kedokteran Bandung Vol 44, No 2 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Kadar N-terminal pro-brain natriuretic peptide (NT-proBNP) plasma dapat menggambarkan tingkat keparahan iskemia walaupun tidak terjadi nekrosis, iskemia yang transien dapat meningkatkan peregangan dinding jantung yang akan menginduksi sintesis dan pelepasan brain natriuretic peptide (BNP) yang sebanding dengan tingkat keparahan iskemia. Tujuan penelitian untuk mengetahui apakah kadar NT-proBNP pada penderita sindrom koroner akut (SKA) dapat digunakan sebagai parameter prediktor luaran klinis. Penelitian dilakukan sejak bulan Januari hingga Maret 2010. Subjek penelitian penderita SKA yang datang ke Unit Gawat Darurat Rumah Sakit Dr. Hasan Sadikin Bandung dan telah didiagnosis klinis sesuai kriteria World Health Organization. Pada subjek yang memenuhi kriteria inklusi dilakukan pemeriksaan kadar NT-pro BNP dengan metode electrochemiluminescence immunoassay, cardiac troponin T (kuantitatif), dan creatine kinase muscle brain (enzimatik). Analisis data uji normalitas menggunakan one-sample Kolmogorov-Smirnov test, analisis regresi logistik multipel untuk mengetahui parameter prediktor luaran klinis penderita SKA. Dari 83 subjek yang ikut dalam penelitian, didapatkan nilai prediksi kadar NT-proBNP sebesar 1,00 sehingga bukan merupakan prediktor utama luaran klinis, koefisien β NTproBNP sebesar 0,001 menyatakan bahwa setiap penambahan 1.000 pg/mL variabel NT-proBNP akan menambah lama perawatan 1 hari. Pada subjek SKA dengan luaran (outcome) sembuh nilai prediksi cTnT lebih baik sebagai faktor prediktor dibandingkan dengan konsentrasi NT-proBNP (OR=32,53; 95%IK; 0,58–1.819,26). Simpulan, NT-proBNP bukan merupakan prediktor utama luaran klinis pada SKA. Kadar NT-proBNP lebih dari 826,7 pg/mLterdapat kemungkinan prognosis yang buruk sampai dengan kematian. [MKB. 2012;44(2):106–13].The Role of NT-proBNP as Clinical Outcome Predictor for Acute Coronary SyndromesPlasma levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) levels may reflect the severity of ischemia, although there is no necrosis. A transient ischemia which can increase the heart wall stretch would induces BNP synthesis and release. Synthesis and release of BNP are comparable with the severity of ischemia. The aim of this study was to analyze whether NT-proBNP levels in patients with acute coronary syndrome (ACS) can be used as a predictor for clinical outcome. Studies was held since January to March 2010. Subject were patients with ACS who came to emergency room Dr. Hasan Sadikin Hospital Bandung and were clinically diagnosed according to World Health Organization criteria. Subjects which were suited with the inclusion criteria, stored until assayed. NT-pro BNP concentration was examined by electrochemiluminescence immunoassay method along with creatine kinase muscle brain (enzymatic method) and cardiac troponin T (quantitative method). Statistical analysis was performed using the one-sample Kolmogorov-Smirnov test for verifying normality, normally distributed data were analyzed using parametric analysis and abnormal distributed data was assayed using multiple logistic regression analysis to determine the parameters which can be used as predictor for clinical outcome in patients with ACS. Multiple logistic regression analysis on 83 subjects showed predictive value of NT-proBNP levels with OR=1.00, which mean there was no different likelihood in patients with high and low concentration of NT-proBNP to have longer hospitality duration. NT-proBNP β coefficient of 0.001 states that every addition of 1,000 pg/mL of NT-proBNP concentration will increase the length of hospitality duration for one day. On convalesce subjects, the most significant predictive value for predicting clinical outcome cTnT was more better than NT-proBNP concentration in patients with ACS (OR=32.53, 95%CI; 0.58–1,819.26). In conclusions, NT-proBNP is not a major predictor of clinical outome in ACS. NT-proBNP levels of >826.7 pg/mL implies a poor prognosis to death. [MKB. 2012;44(2):106–13]. DOI: http://dx.doi.org/10.15395/mkb.v44n2.132 
Kesesuaian Hasil Pemeriksaan Antibodi Virus Herpes Simpleks Metode Enzyme-Linked Immunofiltration Assay dengan Enzyme-Linked Immunosorbent Assay Immanuel, Victor; Noormartany, Noormartany; Dewi, Nina Susana
Majalah Kedokteran Bandung Vol 44, No 3 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Infeksi virus herpes simpleks (HSV) merupakan infeksi yang disebabkan oleh HSV tipe 1 (HSV-1) dan HSV tipe 2 (HSV-2). HSV-1 biasanya menyebabkan penyakit orofasial, sedangkan HSV-2 biasanya menyebabkan infeksi perigenital. Diagnosis infeksi HSV ditegakkan berdasarkan anamnesis, pemeriksaan fisis, dan laboratorium. Metode deteksi anti-HSV metode enzyme-linked immunosorbent assay (ELISA) memiliki sensitivitas 93–100% dan spesifisitas 95–100%, sedangkan metode enzyme-linked immunofiltration assay (ELIFA) memiliki sensitivitas 83,36–97% dan spesifisitas 83,93–98%. Tujuan penelitian adalah menilai kesesuaian hasil pemeriksaan anti-HSV antara metode ELIFA dan ELISA. Bila terdapat kesesuaian yang baik maka metode ELIFA dapat menggantikan metode ELISA. Penelitian dilakukan di laboratorium klinik RSUP Dr. Hasan Sadikin Bandung sejak bulan Januari–Mei 2011. Rancangan penelitian adalah potong lintang. Subjek penelitian adalah serum penderita tersangka infeksi HSV. Dilakukan analisis statistik untuk menilai agreement Kappa. Sebanyak 66 sampel diperiksa anti-HSV metode ELIFA dan ELISA. Hasil pemeriksaan IgM anti-HSV antara metode ELIFA dan ELISA memiliki kesesuaian baik (p<0,001; K=0,621), hasil pemeriksaan IgG anti-HSV-1 antara metode ELIFA dan ELISA memiliki kesesuaian sedang (p<0,001; K=0,533), dan hasil pemeriksaan IgG anti-HSV-2 antara metode ELIFA dan ELISA memiliki kesesuaian kurang (p=0,006; K=0,260). Simpulan, hanya pemeriksaan IgM anti-HSV metode ELIFA yang memiliki hasil kesesuaian baik dengan metode ELISA, sedangkan pemeriksaan IgG anti-HSV metode ELIFA memiliki kesesuaian sedang atau kurang. [MKB. 2012;44(3):152–8].Kata kunci: IgM anti-HSV, IgG anti-HSV, kesesuaian, metode ELIFA, metode ELISA Agreement of Herpes Simplex Virus Antibody Test Result between Enzymelinked Immunofiltration and Enzyme-linked Immunosorbent Assay MethodsHerpes simplex virus (HSV) infections are very common and are caused by HSV type 1 (HSV-1) and HSV type 2 (HSV-2). HSV-1 being mostly associated with orofacial disease, whereas HSV-2 is usually associated with perigenital infection. Diagnosis of HSV infection is established based on history, physical and laboratory examination. Enzyme-linked immunosorbent assay (ELISA) method to detect anti-HSV has a sensitivity 93–100% and specificity 95–100%, whereas enzyme-linked immunofiltration assay (ELIFA) has a sensitivity 83.36–97% and specificity 83.93–98%. The aim of this study was to assess the agreement of anti-HSV between ELIFA and ELISA methods. This study was conducted in the clinical laboratory RSUP Dr. Hasan Sadikin Bandung since January to May 2011. The study design was cross sectional. Subjects of this study were serum of patients suspected HSV infection. Statistical analysis was performed to assess Kappa agreement. A total of 66 samples were examined anti-HSV using ELIFA and ELISA method. There was good agreement between test results of anti-HSV IgM ELIFA and ELISA method (p<0.001, κ=0.621), moderate agreement between test results of anti- HSV-1 IgG ELIFA and ELISA method (p<0.001, κ=0.533), and fair agreement between test results of anti-HSV-2 IgG ELIFA and ELISA method (p=0.006, κ= 0.260). In conclusions, only the anti-HSV IgM ELIFA method has good agreement with ELISA. DOI: http://dx.doi.org/10.15395/mkb.v44n3.86 
Perbedaan Kadar Osteoprotegerin Serum Penderita Diabetes Melitus Tipe 2 tanpa dan dengan Penyakit Jantung Koroner Nestiti, Ratri Dwitiya; Noormartany, -; Dewi, Nina Susana; Rachmayati, Sylvia
Majalah Kedokteran Bandung Vol 47, No 4 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Diabetes melitus (DM) tipe 2 merupakan diabetes yang disebabkan oleh resistensi insulin dan atau gangguan sekresi insulin sehingga menyebabkan hiperglikemia yang merupakan penyebab disfungsi endotel. Disfungsi endotel merupakan proses awal aterosklerosis dan merupakan salah satu faktor risiko yang menyebabkan penyakit jantung koroner (PJK). Sampai saat ini angiografi koroner digunakan sebagai baku emas PJK. Pemeriksaan osteoprotegerin (OPG) merupakan pemeriksaan alternatif sebagai penanda disfungsi endotel, kurang invasif, lebih dini, dan murah. Osteoprotegerin berhubungan dengan perkembangan plak arteri pada penderita DM tipe 2. Tujuan penelitian ini mengetahui perbedaan kadar OPG dalam serum penderita DM tipe 2 tanpa dan dengan PJK. Penelitian dilakukan pada September–Desember 2012 di Poliklinik Endokrin dan Instalasi Gawat Darurat RSUP Dr. Hasan Sadikin Bandung. Subjek penelitian sebanyak 102 orang, 51 orang penderita telah didiagnosis oleh klinisi sebagai penderita DM tipe 2 dengan PJK dan 51 orang penderita DM tipe 2 tanpa PJK, berusia 35–75 tahun. Pemeriksaan OPG menggunakan metode enzyme linked immunosorbent assay (ELISA). Bentuk penelitian adalah observasional komparatif dengan rancangan penelitian studi perbandingan potong lintang. Analisis statistik menggunakan uji Shapiro Wilk’s, independent T, dan Mann Whitney. Nilai median kadar OPG serum untuk penderita DM tipe 2 dengan PJK lebih tinggi, yaitu sebesar 6,1 pmol/L dan penderita DM tipe 2 tanpa PJK, yaitu sebesar 2,6 pmol/L (p=0.0001). Simpulan, terdapat perbedaan kadar OPG serum antara kelompok DM tipe 2 tanpa dan dengan PJK. [MKB. 2015;47(4):218–23]Kata kunci: Disfungsi endotel, DM tipe 2, osteoprotegerinDifferences of Osteoprotegerin Levels in Patients with Type 2 Diabetes Mellitus with and without Coronary Arterial DiseaseAbstractType 2 diabetes mellitus (DM) is caused by insulin resistance and/or impaired insulin secretion, thus causing hyperglycemia leading to endothelial dysfunction. Endothelial dysfunction is an early process of atherosclerosis and is one of the risk factors for coronary arterial disease (CAD). Currently, coronary angiography is used as the gold standard for diagnosing CAD. Examination of osteoprotegerin (OPG) is an alternative assay for endothelial dysfunction marker which is less invasive, has the ability to detect earlier, and less expensive. Osteoprotegerin is associated with the development of arterial plaque in patients with type 2 DM. The aim of this study was to determine the differences in serum OPG levels in patients with type 2 DM with and without CAD. The study was conducted in September–December 2012 in the Endocrine Clinic and Emergency Unit of Dr. Hasan Sadikin General Hospital Bandung on 102 patients aged between 35–75 years, with 51 subjects type 2 DM patients with CAD and 51 subjects with type 2 DM without CAD, both diagnosed by clinician. The method used was, OPG enzyme linked immunosorbent assay (ELISA) examination method. The study was an observational cross-sectional comparative study. Statistical analysis was performed using normality test with Shapiro Wilk’s, independent T and Mann Whitney test. The median value for patients with type 2 DM with CAD equaled to 6.1 pmol/L and the value for type 2 diabetic patients without CAD was 2.6 pmol/L. In this study, the OPG levels obtained in patients type 2 DM with CAD were higher than those without CAD. In conclusion, there is a significant difference in OPG levels between type 2 DM with and without CAD groups. [MKB. 2015;47(4):218–23]Key words: Endothelial dysfunction, type 2 diabetes, osteoprotegerin DOI: 10.15395/mkb.v47n4.616
Deteksi Natrium/Iodide Symporter (NIS) pada Galur Sel Kanker Payudara SKBR3 dengan Imunositofluoresens Elliyanti, Aisyah; Wikayani, Tenny Putri; Noormartany, Noormartany; Masjhur, Johan S.; Achmad, Tri Hanggono
Majalah Kedokteran Bandung Vol 48, No 1 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Galur sel SKBR3 adalah model kanker payudara positif human epidermal growth factor receptor2 (HER2). Pemberian kemoterapi memperlihatkan respons lengkap hanya pada 50% pasien kanker payudara dengan tipe positif HER2. Kemampuan jaringan tumor menangkap dan mengakumulasi iodium radioaktif dihubungkan dengan ekspresi natrium/iodide symporter (NIS). Tujuan penelitian ini adalah menilai ekspresi dan distribusi NIS pada galur sel SKBR3 serta menilai efek induksi epidermal growth factor (EGF) pada ekspresi NIS menggunakan imunositofluoresens-ISF. Penelitian ini dilakukan di Laboratorium Kultur Sel, Fakultas Kedokteran Universitas Padjadjaran (FKUP) mulai bulan September 2013 sampai dengan April 2014. Sel SKBR3 ditumbuhkan pada plat kultur dan ditunggu hingga konfluen 70%. Sel dibagi atas dua kelompok, yaitu kelompok yang diberi induksi dan kontrol. Induksi EGF diberikan dengan dosis 50 ng/mL. Pemeriksaan ISF menggunakan antibodi primer rabbit polyclonal antibody anti NIS dan antibodi sekunder goat anti rabbit IgG polyclonal antibody. Data hasil pengamatan dinilai secara semikuantitatif. Natrium/iodide symporter tampak terekspresi dan terdistribusi di sitoplasma. Sel yang diinduksi dengan EGF memperlihatkan peningkatan ekspresi NIS di sitoplasma dan distribusinya di membran sel secara bermakna. Sel SKBR3 mengekspresikan NIS yang terdapat di sitoplasma. Induksi EGF meningkatkan ekspresi NIS dan distribusinya di membran sel. Temuan ini dapat mengarah potensi kemampuan sel kanker payudara menangkap dan mengakumulasikan iodium radioaktif. [MKB. 2016;48(1):15–8] Kata kunci: Ekspresi NIS , galur sel SKBR3, kanker payudara, imunositofluoresensDetection of Natrium/Iodide Symporter (NIS) in SKBR-3 Breast Cancer Cell Line Using ImmunocytofluoresenceAbstractSKBR-3 cell line is a breast cancer model for human epidermal growth factor receptor2 (HER2) positive. Only 50% of patients of this type have fully responded to chemotherapy. Natrium iodide symporter expression correlates with the uptake and ability of cells to accumulate radioiodine. The aim of this study was to examine natrium/iodide symporter (NIS) expression and its distribution with and without epidermal growth factor (EGF) treatment using immunocytofluoresence (ICF). This study was conducted at the Cell Culture Laboratory, Faculty of Medicine, Universitas Padjadjaran from September 2013 to April 2014. SKBR3 cells were cultured until 70% confluent. Cells were then divided into two groups: treatment group and control group. The treatment group was treated with EGF 50 ng/mL. Cells were incubated with primary antibody rabbit polyclonal antibody anti-NIS, and then were followed with secondary-antibody goat polyclonal antibody to rabbit. Data from the observation were then assessed semi-quantitatively. Natrium/iodide symporter was seen to be expressed and distributed in the cytoplasm. Cells induced by EGF showed significant increase in NIS expression in cytoplasm and its distribution in cell membrane. It is concluded that the SKBR3 cells express NIS in cytoplasm and that EGF induction increases NIS expression and distribution in cell membrane. This finding leads to a potential ability of breast cancer cells to uptake and accumulate radioiodine. [MKB. 2016;48(1):15–8]Key words: Breast cancer, cell line SKBR-3, immunocytofluoresence, NIS expression
Prothrombin Time, Activated Partial Thromboplastin Time, Fibrinogen, dan D-dimer Sebagai Prediktor Decompensated Disseminated Intravascular Coagulation Sisseminated pada Sepsis Fenny, -; Dalimoenthe, Nadjwa Zamalek; Noormartany, -; Pranggono, Emmy; Dewi, Nina Susana
Majalah Kedokteran Bandung Vol 43, No 1
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

AbstrakSepsis adalah respons sistemik terhadap infeksi dan terutama terjadi pada pneumonia. Sepsis dapat menyebabkan komplikasi disseminated intravascular coagulation (DIC) yang dibedakan menjadi compensated dan decompensated DIC. Tujuan penelitian ini adalah untuk menentukan apakah nilai prothrombin time (PT), activated partial thromboplastin time (aPTT), kadar fibrinogen, dan D-dimer dapat digunakan sebagai prediktor decompensated DIC pada penderita sepsis. Penelitian dilakukan di Laboratorium Patologi Klinik Rumah Sakit Hasan Sadikin Bandung mulai September 2008 sampai Juni 2010. Subjek penelitian adalah penderita sepsis yang disebabkan pneumonia. Nilai PT, aPTT, kadar fibrinogen, dan D-dimer semua subjek sepsis dicatat kemudian dilakukan pengamatan sampai subjek dinyatakan mengalami decompensated atau non-decompensated DIC; selanjutnya dilakukan analisis nilai PT, aPTT, kadar fibrinogen, dan D-dimer pada kelompok decompensated dan non-decompensated DIC. Penelitian menggunakan rancangan cohort. Subjek berjumlah 39 orang (58%) penderita sepsis dengan luaran decompensated DIC dan 28 orang (42%) penderita sepsis dengan luaran non-decompensated DIC. Dari parameter hemostasis yang diperiksa, didapatkan bahwa nilai PT, aPTT, dan fibrinogen merupakan prediktor decompensated DIC pada penderita sepsis dengan risiko relatif (RR) masing-masing 240,500; 7,157; dan 6,421. Simpulan, prothrombin time, aPTT, dan fibrinogen merupakan pemeriksaan untuk mengetahui aktivasi koagulasi. Parameter hemostasis yang merupakan prediktor decompensated DIC pada penderita sepsis adalah nilai PT dan aPTT yang memendek serta kadar fibrinogen yang meningkat. [MKB. 2011;43(1):49–54].Kata kunci: Activated partial thromboplastin time, D-dimer, disseminated intravascular coagulation, fibrinogen, prothrombin time, sepsisProthrombin Time, Activated Partial Thromboplastin Time, Fibrinogen, and D-dimer as a Predictor of Decompensated Disseminated Intravascular Coagulation in SepsisSepsis is a systemic response to infection especially in pneumonia case. Sepsis can cause complications such as disseminated intravascular coagulation (DIC) which can be divided into compensated and decompensated DIC. The purpose of this study was to assess whether the value of prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen, and D-dimer levels can be used as predictors of decompensated DIC in sepsis patients. This study was conducted at the Laboratory of Clinical Pathology Rumah Sakit Hasan Sadikin Bandung since September 2008 to June 2010. Subjects were patients with sepsis caused by pneumonia. PT and aPTT values, fibrinogen, and D-dimer levels was recorded from all sepsis patients then patients were observed until diagnosed decompensated or non-decompensated DIC, then the value of PT, aPTT, fibrinogen and D-dimer levels in the group of decompensated DIC and non-decompensated DIC were analysed. This study used cohort design. Subjects were 39 sepsis patients (58%) with outcome decompensated DIC and 28 sepsis patients (42%) with outcome non-decompensated DIC. From the hemostasis parameter test out, it was found that PT, aPTT, and fibrinogen were the predictor of decompensated DIC in patients with sepsis with relative risk 240.500, 7.157, and 6.421; respectively. Conclusions, prothrombin time, aPTT, fibrinogen are the test to know coagulation activation. Hemostasis parameter to predict decompensated DIC in sepsis patients are the shorten PT, aPTT, and the increased fibrinogen. [MKB. 2011;43(1):49–54].Key words: Activated partial thromboplastin time, D-dimer, disseminated intravascular coagulation, fibrinogen, prothrombin time, sepsi DOI: http://dx.doi.org/10.15395/mkb.v43n1.44
Perbedaan Kadar Liver Fatty Acid Binding Protein (L-FABP) Urine Penderita Diabetes Melitus Tipe 2 dengan Normoalbuminuria dan Mikroalbuminuria Wiharjo, Kristina; Permana, Hikmat; Noormartany, -; Rachmayati, Sylvia
Majalah Kedokteran Bandung Vol 46, No 2 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Diabetes melitus (DM) merupakan salah satu penyebab end stage renal disease (ESRD), 20−40% akan mengalami nefropati diabetik yang berkembang menjadi ESRD. Prevalensi nefropati diabetik meningkat pada DM tipe 2. Akumulasi stres oksidatif dapat menyebabkan peningkatan permeabilitas glomerulus sehingga menyebabkan peningkatan ekskresi albumin pada urine yang terbagi menjadi normoalbuminuria, mikroalbuminuria, dan makroalbuminuria. Kerusakan glomerulus terjadi setelah kerusakan tubulointerstisial ginjal yang menyebabkan penurunan aliran kapiler peritubular dan menyebabkan hipoksia. Liver fatty acid binding protein (L-FABP) berfungsi mengurangi hipoksia dengan mengikat stres oksidatif dan mengeluarkannya ke dalam urine. Penelitian ini bertujuan untuk menganalisis apakah terdapat perbedaan kadar L-FABP antara penderita DM tipe 2 normoalbuminuria dan mikroalbuminuria. Metode penelitian adalah observasional analitik dengan rancangan potong lintang. Subjek penelitian sebanyak 70 orang penderita DM tipe 2 dengan normoalbuminuria (38 orang) dan mikroalbuminuria (32 orang) yang diambil secara acak pada pasien yang datang ke Laboratorium Rawat Jalan Rumah Sakit Dr. Hasan Sadikin pada periode Juli−September 2012. Uji statistik menggunakan Uji Mann-Whitney. Hasil penelitian menunjukkan perbedaan bermakna kadar L-FABP urine pada normoalbuminuria dengan mikroalbuminuria dengan nilai ZM-W=3.513; p<0,001, median pada normoalbuminuria adalah 5, dan mikroalbuminuria adalah 7. Dari penelitian ini didapatkan kadar L-FABP urine meningkat karena kompensasi pembentukan stres oksidatif dan hipoksia yang terjadi sebelum kerusakan glomerulus. Simpulan, kadar L-FABP urine pada pasien DM tipe 2 dengan mikroalbuminuria lebih tinggi daripada pasien DM tipe 2 dengan normoalbuminuria. [MKB. 2014;46(2):83–7]Kata kunci: Diabetes melitus tipe 2, kadar L-FABP urine, mikroalbuminuria, nefropati diabetik, normoalbuminuria Comparison of Urinary Liver-Fatty Acid Binding Protein (L-FABP) in Normoalbuminuria and Microalbuminuria in Type 2 Diabetes Mellitus PatientsDiabetes mellitus (DM) is the leading cause of the end stage renal disease (ESRD). Around 20−40% patients with DM develop diabetic nephropathy and eventually progress into ESRD. Type 2 DM has a greater prevalence to develop diabetic nephropathy. Oxidative stress accumulation can increase permeability of the glomerulus which results in increased urine albumin excretion, which is divided into three groups: normoalbuminuria, microalbuminuria and macroalbuminuria. Glomerulus dysfunction occurs after tubulointerstisial renal dysfunction which decreases peritubular capillary flow that leads to tubulointerstisial hypoxia. Liver fatty acid binding protein function is to reduce hypoxia by binding oxidative stress and excretes it into urine. The aim of this study was to analyze the differences in the urine L-FABP level between normoalbuminuria and microalbuminuria type 2 DM patients. The study design was observational analytic using cross-sectional method. Subjects were 70 DM type 2 patients with normoalbuminuria (38 patients) and microalbuminuria (32 patients). Statistical analysis was performed using the Mann Whitney test The results found that there were significant differences in levels of urine L-FABP between normoalbuminuria and microalbuminuria type 2 DM patients (ZM-W=3.513, p<0.001) with medians of 5 and 7 in normoalbuminuria and microalbuminuria, respectively. The urine L-FABP level increased because of the oxidative stress and hypoxia that happened before the glomerulus dysfunction. In conclusion, urine L-FABP level in patients DM type 2 with microalbuminuria is higher than that of the normoalbuminuria. [MKB. 2014;46(2):83–7]Key words: diabetic nephropathy, L-FABP urine level, microalbuminuria, normoalbuminuria, type 2 DM DOI: 10.15395/mkb.v46n2.278
Co-Authors - Syuhada, - Achmad Mauludin Agista Rohani Agnes R Indrati, Agnes R Agnes R. Indrati Aisyah Elliyanti Alfiyya Nur Mahdiyyah Amelia Rachmiwatie Arief Budi Yulianti Ariko Rahmat Putra Arto Yuwono Soeroto Coriejati Rita Dany Hilmanto Delita Prihatni Dewi Susanna Emmy Pranggono Fenny, - Florencia Idajanti Tandhana Hikmat Permana HY Yusup HY Yusup, HY Ida Parwati Indahwaty, - Ismet Muchtar Nur Ismu Huzen Ivana Agnes Sulianto Ivana Agnes Sulianto, Ivana Agnes Johan S. Masjhur, Johan S. Kristina Wiharjo Lelly Yuniarti Liliana A Lulu Fahrizah Balqis, Lulu Fahrizah M. Ruslin M. Ruslin, M. Maya Tejasari Moudy Emma Unaria Djami Muhammad Alamsyah Muhammad Arkan Bastian MZ Arifin MZ Arifin Nadjwa Zamalek Dalimoenthe neli sumanti Nina Susana Dewi Nina Tristina Puspita, Sara R. Anita Indriyanti Raden Anita Indriyanti Ratri Dwitiya Nestiti, Ratri Dwitiya Rika Nilapsari Rosady, Dony Septriana Rubin Surachno Gondodiputro Rudi Wisaksana Salma Aulya Fitriani Siti Annisa Devi Trusda, Siti Annisa Devi Sugianli AK Sylvia Rachmayati Tenny Putri Wikayani, Tenny Putri Tiene Rostini, Tiene Toni M. Aprami Tri Hanggono Achmad Victor Immanuel Yani Triyani Yenolinsky, Yolanda Yuktiana Kharisma