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All Journal Journal of Health (JoH) Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat JPM (Jurnal Pemberdayaan Masyarakat) Jurnal Analis Kesehatan Jurnal Kesehatan Medical Technology and Public Health Journal Medica Hospitalia Medicra (Journal of Medical Laboratory Science/Technology) Academia Open Humanism : Jurnal Pengabdian Masyarakat Medical Scope Journal (MSJ) Jurnal Abdimas Kartika Wijayakusuma Indonesian Journal of Innovation Studies Procedia of Social Sciences and Humanities Journal of Noncommunicable Diseases (JOND) Prosiding University Research Colloquium Indonesian Journal Health Science and Medicine Folia Medica Indonesiana
Andika Aliviameita
Universitas Muhammadiyah Sidoarjo
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : Medical Laboratory Technology Journal

 1 
Detection of Salmonella typhi Using Multiplex and Monoplex PCR in Tifoid Fever Patients Andika Aliviameita; Meryn Linanda Agustin; Anggie Wedha Puspita; Miftahul Mushlih; Puspitasari Puspitasari; Yanik Purwanti; Arief Wisaksono
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (700.217 KB) | DOI: 10.31964/mltj.v5i2.230

Abstract

Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.
The Effects of Hyperbaric Oxygen Therapy on Blood Glucose Levels and Lipid Profile in Diabetes Mellitus Patients Andika Aliviameita; Nevy Elensya; Puspitasari Puspitasari; Sri Mukhodim Faridah Hanum
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (545.458 KB) | DOI: 10.31964/mltj.v6i2.307

Abstract

Diabetes mellitus and its complications are still a major health problem in the world. Diabetes mellitus is closely related to the lipid profile, especially concerning the cardiovascular disease. A method that can be used to treat diabetes mellitus is hyperbaric oxygen therapy (HBOT). This study aims to observe changes in blood glucose levels and lipid profiles in patients with diabetes mellitus with hyperbaric oxygen therapy. The determination of blood glucose levels and lipid profiles is done by photometry. A total of 30 patients with diabetes mellitus examined for their blood glucose level and lipid profile before and after hyperbaric oxygen therapy. In the Paired T-test, there were significant decrease in blood glucose levels (p= 0,000), total cholesterol (p= 0,000), and triglycerides (p= 0,000) after hyperbaric oxygen therapy. The conclusion of this study shows there is a significant decrease in blood glucose level and lipid profile (total cholesterol, and triglycerides) after hyperbaric oxygen therapy.
Quality of Genom in Type II Diabetes Mellitus Patients in Viewed of Temperature, Storage Duration, Number of Leukocyte Puspitasari Puspitasari; Evi Rinata; Rohman Dijaya; Siska Aprilia; Dina Trikumalasari; Livia Nur Azzah; Qilmia Fanani; Miftahul Mushlih; Andika Aliviameita; Dian Delta
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.348 KB) | DOI: 10.31964/mltj.v5i2.229

Abstract

Type II Diabetes Mellitus is a disorder caused by genetic and environmental factors. Molecular analysis of T2DM abnormalities has been carried out. But the analysis in sample preparation especially the stages of DNA isolation has not been done much. The aims of this study are to investigate the effect of temperature, storage duration and level of white blood cell (WBC) with Genome Quality in T2DM. The treatment (n:10) which were divided into several tubes and then stored at 4 °Ϲ, 25 °Ϲ and 32 °Ϲ for 21 days. To determining storage duration effect, we use periodically isolation of DNA (3, 15 and 30 days) after sampling. The effect of WBC with DNA quality was carried out using 17 samples. DNA isolation was done by the DNA Isolation Kit manual without modification and then tested qualitatively and quantitatively. Based on this research, it can be concluded there is a correlation between the numbers of WBC with DNA quality. The higher the number of WBC, the higher DNA concentration (r: 0.818. p value: 0.000). The concentration of DNA at a temperature of 4°Ϲ (135.1 ± 165.2 ng / µl) was higher compared with the temperature treatment 25 ° Ϲ (29.7 ± 36.5 ng / µl) and 32 ° Ϲ (22.14 ± 7.13 ng / µl) (p
Co-Authors Ahmad Shobrun Jamil Ahmad Yudianto Ainutajriani, Ainutajriani Alda Megawati Amilya, Widya Intan Anggie Wedha Puspita ARDIANSYAH, SYAHRUL Arief Wisaksono Ariyanti, Silvia Asri, Nur Indah Nila Aziza, Vidayatul Batis, Afifah Diah Ariana Dian Delta Dina Trikumalasari Dira Nadila Ellies Tunjung Sari Maulidiyanti, Ellies Tunjung Sari Emilia, Hilda Evi Rinata Fadilatin, Lisa Anis Firmansyah, Nukayo Fitrian Desi Prameswari Hadie, Djauharoh A Intantri, Risma Jamilatur Rohmah Krisdianti, Rika Lestari, Dzulia Livia Nur Azzah Meryn Linanda Agustin Mieke Sylvia Miftahul Mushlih Millah, Hamidah Wardatul Murosidah, Pratasya Liyaajul Mushlih , Miftahul Nevy Elensya NURUL AZIZAH p Puspitasari, p Primastuti, Yuridistya Putri Puspita, Anggun Puspitasari Puspitasari Puspitasari Puspitasari Qilmia Fanani Ramadhan, Syahrul Ibnu Mubaroq Riantika, Mayang Rini, Chylen S. Rini, Chylen Setyo Rohman Dijaya Saidah, Sukma Nur Salim, Siti Zaenab Savitra, Igfina El Segara, Bayu Setiyo Rini, Chylen Silvia Ariyanti Siska Aprilia Siti Zaenab Salim Sri Mukhodim Faridah Hanum Wahyuni, Nining Xena, Alfanny Juliannindy Yanik Purwanti Yasmin, Rizka Afifah Yonika Zakaria, Rizal