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GC-MS (Gas Chromatography-Mass Spectrometry) Metabolit Sekunder Ekstrak Etanol dan Metanol Daun Kirinyuh (Chromolaena odorata L.) Florian Mayesti Prima Remba Makin; Ite Morina Yostianti Tnunay; Gede Arya Wiguna
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.6519

Abstract

Kirinyuh (Chromolaena odorata L.) is a woody shrub plant that can grow quickly so it is an aggressive competitor. Traditionally, kirinyuh leaves have been used as a wound healing medicine, treating sore throat, malaria, headache, anti-diarrheal, astringent, antiplasmodial, antihypertensive, and antiinflammatory. This study aims to determine the content of secondary metabolites of kirinyuh leaves using ethanol and methanol solvents. This type of research is qualitative research. The results showed that the ethanol solvent displayed 1 peak with the possibility of 3 secondary metabolites with a concentration of 100%, namely Germacrene D $$ 1,6-Cyclodecadiene, 1-methyl-5-methylene-8-(1-methylethyl)-, [s-(E,E)]- (CAS) Germacra-1(10),4(15),5-triene, (-)- (CAS) (-)-Germacrene D $$; Alpha.-Amorphene $$ .ALPHA. AMORPHENE $$ Naphthalene, 1,2,4a,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-, [1S-(1.alpha.,4a.beta.,8a.beta.)]- (CAS) 6.alpha.-Cadina-4,9-diene, (-)- (CAS) 6.alpha.-Cadina-4,9-diene $$ .alpha.-Amorphene, (-)- $$ (-)-.alpha.-Amorphene $$, and Calarene $$ 1H-Cyclopropa[a]naphthalene, 1a,2,3,5,6,7,7a,7b-octahydro-1,1,7,7a-tetramethyl-, [1aR (1a.alpha.,7.alpha.,7a.alpha.,7b.alpha.)]- (CAS) .beta.-Gurjunene $$ 1(10)-Aristolene, (+)- $$ .delta.1(10)-Aristolene $$ 1H Cyclopropa[a]naphthalene, 1a,2,3,5,6,7,7a,7b-octahydro-1,1,7,7a-tetramethyl-, (1aR,7R,7aR,7bS)-(+)- $$ BETA-GURJUNENE $$ (+)-Calarene $$ (+)-.DELTA.1(10)-Aristolene $$ (+)-1(10)-Aristolene $$. Methanol solvent produces 3 peaks, number 3 is the highest peak with a concentration of 77.31% with the possibility of 5 compounds namely Iron, (.eta.5-2,4-cyclopentadien-1-yl)[(1,2,3,3a,7a-.eta.)-4,5,6,7-tetrahydro-4-hydroxy-5-methyl-1H-inden-1-yl]-, stereoisomer (CAS); 2,4-diphenyl-2-hydroxymethylpyrano[2,3-b][1]benzopyran-5-one $$ 2H,5H-Pyrano[2,3-b][1]benzopyran-5-one, 3,4-dihydro-2-(hydroxymethyl)-2,4-diphenyl- (CAS); Kauran-18-oic acid, 7-(acetyloxy)-15,16-epoxy-, methyl ester, (4.alpha.,7.beta.,15.alpha.)- (CAS) METHYL 7.BETA.-ACETOXY-15,16 .ALPHA.-EPOXY-16.BETA.-(-)-KAURAN-18-OATE $$; Stannane, tetrakis(1-methylethyl)- (CAS) Tetraisopropyltin $$ Tetraisopropylstannane $$ Stannane, tetraisopropyl- $$ (iso-C3H7)4Sn $$ tetra-i-Propyltin $$; and Cholest-5-en-3-ol (3.beta.)- (CAS) Lanol $$ Dythol $$ Kathro $$ Dusoran $$ Cordulan $$ Dusoline $$ Hydrocerin $$ Cholesterin $$ Cholesterol $$ Tegolan $$ (-)-Cholesterol $$ Provitamin D $$ Cholesterol base H $$ Wool alcohols B. P. $$ Cholesteryl alcohol $$ Cholest-5-en-3.beta.-ol $$ Nimco cholesterol base H $$ 5:6-Cholesten-3.beta.-ol $$ 3.beta.-Hydroxycholest-5-ene $$ Cholest-5-en-3-ol, (3.beta.)- $$ Nimco cholesterol base No. 712 $$ .DELTA.5-Cholesten-3.beta.-ol $$ 5-Cholesten-3.beta.-ol $$ Cholestrin $$. The contents of the secondary metabolites of kirinyuh leaves extracted from ethanol and methanol solvents have different types, amounts, and compositions.
Pelatihan Pembuatan Preparat Segar Bagi Guru Biologi Sekolah Menengah Atas Welsiliana Welsiliana; Gede Arya Wiguna; Florian Mayesti Prima Remba Makin; Ite Morina Yostianti Tnunay; Lukas Pardosi; Dicky Frengky Hanas
Society : Jurnal Pengabdian Masyarakat Vol 2, No 3 (2023): Mei
Publisher : Edumedia Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55824/jpm.v2i3.276

Abstract

Learning media is anything that can be used to convey messages or information in the teaching and learning process so that it can stimulate students' attention and interest in learning. One of the media in biology learning is preparation. Preparations are preparations in the form of organs, tissues, cells, and or bodies of organisms that are preserved or fresh so as to provide convenience for studying, observing, or researching. The purpose of this service activity is to provide training to make fresh preparations for high school biology teachers. The results of this activity include presentation of material on preparations, training on making fresh preparations, and observing fresh preparations that have been made under a microscope. This activity provides new and practical experiences for biology teachers and increases knowledge and enthusiasm for doing simple practicals
Karakterisasi Stomata dan Trikomata Daun Kirinyuh (Chromolaena odorata L.) Florian Mayesti Prima Remba Makin; Welsiliana Welsiliana; Gede Arya Wiguna
Journal Science of Biodiversity Vol 3 No 1: April 2022
Publisher : Program Studi Biologi, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jsb/vol3i1pp61-67

Abstract

Stomata dan trikomata merupakan modifikasi jaringan epidermis. Melalui stomata terjadi pertukaran gas oksigen, karbon dioksida, dan uap air dari daun ke alam sekitar dan sebaliknya. Trikoma biasanya muncul pada hampir seluruh permukaan luar organ tumbuhan baik organ vegetatif maupun organ reproduksi. Kirinyuh (Chromolaena odorata L.) adalah salah satu tumbuhan liar namun secara tradisional, daun kirinyuh telah digunakan sebagai obat dalam penyembuhan luka, mengobati radang tenggorokan, obat malaria, sakit kepala, antidiare, dan astringent, antiplasmodial, antihipertensi dan anti inflamasi. Kelenjar trikomata dapat menghasilkan metabolit sekunder yang dapat dimanfaatkan sebagai herbisida alami. Penelitian ini bertujuan mengkarakterisasi morfologi stomata dan trikomata daun kirinyuh. Metode yang digunakan adalah metode pengolesan aseton. Diperoleh panjang stomata 26,75 µm, lebar stomata 8,95 µm, bentuk stomata oval, sel penutup stomata berbentuk ginjal, dan tipe stomata anomositik. Rata-rata sebanyak 28 stomata ditemukan pada setiap area pengamatan dengan kerapatan stomata 232/mm2. Panjang trikomata daun kirinyuh 205,21 µm dan lebar 29,14 µm. Bentuk trikomata daun kirinyuh adalah multiseluler dan tipe trikomanya adalah non glandular. Ukuran panjang stomata termasuk kategori panjang, sedangkan lebar stomata termasuk kategori kurang lebar. Jumlah stomata daun kirinyuh yang ditemukan masih kategori sedikit dengan kerapatan tergolong rendah. Stomata daun kirinyuh hanya ditemukan pada sisi abaksial daun dan tidak ditemukan pada sisi adaksial, sedangkan trikomata ditemukan ditemukan pada kedua sisi permukaan daun kirinyuh.