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All Journal Hemera Zoa Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Kedokteran Hewan
Rahmat Setya Adji
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Hemera Zoa

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AQ-9 Identification of Sumateran Wild Boar Meat (Sus scrofa vittatus) by Restriction Fragment Length Polymorphism (RFLP) Analysis of Cytochrome b Gene Melani Wahyu Adiningsih; Retno Damayanti Soejoedono; Trioso Purnawarman; Hadri Latif; Rahmat Setya Adji; Okti Nadia Poetri; Dwi Desmiyeni Putri
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Sumateran wild boars have been super abundant in Sumateran forest. In Indonesia, this wildlife condition has led to the exploitation for commercial purpose. The high number of Sumateran wild boars population increases wild boar hunting resulting in an abundant availability of wild boar meat in the food market with extremely cheap price. The macroscopic similarity of wild boar meat and beef has prompted the local people to abuse this situation by selling wild boar meat in traditional market as beef. Based on annual record from Cilegon Class II Quarantine Office in 2014, there were nine smuggling cases or a total of 21.556 kg of wild boar meat smuggling effort that were prevented by Cilegon Quarantine officers. The number of food safety concerns related to smuggling of wild boar or counterfeiting beef with wild boar is a very detrimental condition for consumers, especially consumers in traditional markets.The checking of genuineness or validity of food products is an important effort to protect people from consuming unhealthy food and to indicate whether the food is halal or not. Studies of meat detection should be continuously developed as an effort to protect consumers. Genetic method is the most specific and sensitive method to check food ingredients authenticity by detecting the presence of genetic material or deoxyribonucleic acid (DNA). It results from the specific character of the structure of DNA particles and the possibility of using the information included in them. The most frequent loci used for species identificationin phylogenetics and biodiversity studies are mitochondrial cytochrome b (cyt b).Genetic method is the most specific and sensitive tool for analyzing the authenticity of food ingredients in a molecular level by means of detecting the presence of genetic material or deoxyribonucleic acid (DNA). One of the various methods could be used to detect genetic material is polymerase chain reaction (PCR). Specifically, one of such method frequently used in food industry to observe animal derived product fabrication is PCR restriction fragment length polymorphism (RFLP). PCR-RFLP is based on the comparison of the bands profile generated after certain enzymes digest the DNA target. PCR-RFLP is appropriate for meat testing due its ability in exploiting sequence variation in designated DNA region that allows species differentiation even from closely related species through DNA fragment restrictions selected by suitable restriction enzyme. PCR-RFLP is advantageous since it is simple, cheaper, and easier to be adjusted for routine big-scale studies such as surveillance program.
AQ-12 Application of a Multiplex PCR Assay to Detect Campylobacter fetus subspecies venerealis from Imported Bovine Preputial Samples Mazdani Daulay; Adi Komara; Novera Nirmalasanti; Siti Khadijah; . Marjono; Melyna Sandra; Muhamad Taopik; . Mukromin; . Mustamil; Rahmat Setya Adji
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Campylobacteriosis, caused by Campylobacter spp., is of considerable economic importance to the cattle industry worldwide. Campylobacter spp. were recognized as etiological agents of abortion in sheep. Campylobacter fetus subsp. fetus (Cff) causes sporadic abortion in sheep, often late in gestation, while subspecies venerealis (Cfv) is a cause of sexually transmitted bovine infertility and sporadic abortion in cattle. Various investigations have been carried out in different countries to assess the prevalence and impact of this disease. Some published results surveys are outlined in Table 1. Table 1. A summary of published data showing the prevalence of C. fetus in different countriesStudyareaSample type(s)Sample sizePrevale nce ofC. fetus (%)Diagnostic methodAustralia(1985-1986) Bulls (preputi al suction)1 008animals41 herds87% herdspositiveSerological(Fluorescentantibody test) California(United States of America)Cows40047Serological(ELISA) New ZealandCows (vaginal mucous) and bulls (preputial wash)1 230 cows(125 herds)54 bulls70% herds positiveCfv : 0Cff/othersSerological(ELISA)Bacteriological culture          Cff: Campylobacter fetus subsp. fetus, Cfv: C. fetus subsp. venerealis. According to [1], Bovine Genital Campylobacteriosis (BGC) disease was classified as 1st Group of animal quarantine disease. It is an exotic disease that was not ever detected in Indonesia. However, large scale cattle importation to Indonesia from the countries which ever reported BGC prevalence in their territories, initiating and spreading BGC will be the major threat for feedlotter or dairy farm in Indonesia. Hence, we should apply diagnostic test to detect Cfv in order to prevent the introducing the BGC to Indonesia. The aim of this study was to verify that multiplex PCR assay applicative to detect Campylobacter fetus subsp. venerealis from field samples.
Co-Authors . Marjono . Mukromin . Mustamil Adi Komara Agnesia Endang Tri Hastuti Wahyuni Dwi Desmiyeni Putri Hadri Latif Lily Natalia Maxs Urias Ebenheizer Sanam Mazdani Daulay Melani Wahyu Adiningsih Melyna Sandra Michael Haryadi Wibowo Muhamad Taopik Novera Nirmalasanti Okti Nadia Poetri Retno Damayanti Soejoedono Siti Khadijah Trioso Purnawarman Widagdo Sri Nugroho Widya Asmara