Article Per Year (5 Year)
p-Index From 2021 - 2026
0.702
P-Index
This Author published in this journals
All Journal JBIO: jurnal biosains (the journal of biosciences) Biology, Medicine, & Natural Product Chemistry BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biogenerasi Indonesian Journal of Chemical Science and Technology
Hestia Hairima
State University Of Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
THE DNA SEQUENCE ENCODING GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPC) ENZYME ON TUNTUN ANGIN PLANT (ELAEOCARPUS FLORIBUNDUS BI) Dewi Indriyani Roslim; Hestia Hairima; Herman Herman; Wahyu Lestari
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (498.851 KB) | DOI: 10.31289/biolink.v7i1.3310

Abstract

Glyceraldehyde 3-phosphate dehydrogenase (GapC) is an enzyme involved in glycolysis. The expression of this gene tends to abundant in eukaryotic cells, so this gene is frequently used as an internal control in gene expression analysis. This research aims to isolate the DNA sequence of the GapC gene from tuntun angin (Elaeocarpus floribundus BI). Methods included a collection of the leaves from Kajuik Lake, Riau Province then the DNA extraction, electrophoresis, amplification of partial DNA sequence of GapC gene, cloning and sequencing. The DNA sequence was analyzed using the BLASTn program and MEGA6 software. The GapC sequence obtained in this study was 933 bp in size, consisting of four introns and five exons, and encoding 137 deduced amino acids. The BLASTn analysis showed that the sequence has 89.84%-90.16% similarity to other species of Cunoniaceae family, such as species from the genus of Spiraeanthemum and Codia. The parsial sequence of E. floribundus GapC gene was more resemble the one of Spiraeanthemum than Codia genus. The GapC sequence obtained in this study was the first reported from the Elaeocarpaceae family. This sequence has the opportunity to serve as an internal control after validation.
PENGARUH AIR SUMUR DAN AIR SABUN TERHADAP AKTIVITAS MITOSIS SEL AKAR BAWANG (Allium cepa L. Christian Yopie Daniel Siregar Siagian; Siti Annisa Kusbama; Khory Aulia; Nazla Suhaila Parinduri; Santi Dameana Habeahan; Larasati Arum Utami; Hestia Hairima
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/98kk3z97

Abstract

Mitosis is a cell division process that plays an important role in plant growth. This study aimed to determine the effect of well water and soapy water on the mitotic activity of onion root cells (Allium cepa L.). This research used a descriptive observational method through laboratory practicum with onion roots as the research object. Data were collected through direct observation using a light microscope on mitotic phases of onion root preparations stained with acetocarmine. The results showed that well water produced better root growth and mitotic activity than soapy water. The phases of prophase, metaphase, anaphase, and telophase were more clearly observed in the well water treatment, while fewer dividing cells and damaged tissues were found in the soapy water treatment. It can be concluded that soapy water has a negative effect on the mitotic activity of onion root cells.
In Silico Evaluation of Nitrogen-Containing Tetrahydropyridine and Piperidine Metabolites Identified from Strobilanthes tonkinensis Lindau Leaves as Potential Antibacterial Agents Hestia Hairima; Dwi Sapri Ramadhan; Dian Wardana
Indonesian Journal of Chemical Science and Technology (IJCST) Vol. 9 No. 2 (2026): JULY 2026
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/ijcst.v9i2.68983

Abstract

Strobilanthes tonkinensis Lindau (daun paris), a Southeast Asian aromatic herb, contains several nitrogen-containing piperidine and tetrahydropyridine (THP) metabolites identified by prior GC-MS profiling but not previously evaluated in silico. This study assessed the molecular docking interactions of four such metabolites, 2-propionyl-3,4,5,6-tetrahydropyridine (2-PTHP), 2-acetyl-3,4,5,6-tetrahydropyridine (2-ATHP), 2-(2-furyl)piperidine (2-FP), and 1-(piperidin-2-yl)propan-1-one (PPO) against two bacterial protein targets, FtsA (PDB: 3WT0) and DNA gyrase subunit B (GyrB; PDB: 6KZV), using AutoDock Vina, complemented by ADMET prediction via SwissADME and ProTox 3.0. All four compounds showed moderate binding affinities against FtsA (−5.7 to −5.9 kcal/mol) and GyrB (−5.2 to −6.0 kcal/mol), substantially weaker than the native co-crystallized ligands (−11.5 and −8.9 kcal/mol, respectively). 2-FP exhibited the strongest GyrB affinity (−6.0 kcal/mol) and partially reproduced the native ligand's catalytic-residue contacts (ASP73, ASN46), whereas PPO showed the most catalytically relevant FtsA interaction profile, engaging SER13, VAL211, and ASP210. Structure-activity comparison identified the presence of a piperidine NH (hydrogen bond donor) versus an imine nitrogen as the principal determinant of polar-contact capacity within this scaffold. All compounds fully satisfied Lipinski's Rule of Five, showed high predicted gastrointestinal absorption and blood-brain barrier permeability, and were classified as GHS Acute Oral Toxicity Class IV with inactive predicted hepatotoxicity and carcinogenicity. These findings constitute preliminary computational evidence supporting 2-FP and PPO as candidates for further in vitro antibacterial validation..
GC-MS Profiling and In Silico Antibacterial Activity of Strobilanthes tonkinensis Lindau Leaf Extracts via Maceration and Soxhlet Methods Dwi Sapri Ramadhan; Dian Wardana; Hestia Hairima; Tita Juwitaningsih; Rosa Nadya Evelyn Sitorus; Vina Octavia Azzahra; Ilham Sinuraya
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1575-1594

Abstract

Strobilanthes tonkinensis Lindau (Acanthaceae; locally known as daun paris) is an aromatic medicinal herb of Southeast Asian origin whose extraction-method-dependent secondary metabolite composition and antibacterial potential against defined molecular targets have not been systematically characterized. This study profiled the GC-MS-detectable metabolome of four leaf extracts obtained by ethanol maceration of fresh (3×24 h) and dried leaves (3×24 h), ethanol Soxhlet (13 cycles, 6.5 h), and n-hexane Soxhlet (25 cycles, 10 h) extraction of dried leaves, identifying 19, 27, 12, and 7 compounds, respectively. Extraction method and solvent polarity strongly governed compound class distribution: fresh ethanol maceration was dominated by 2-propionyl-1,4,5,6-tetrahydropyridine (33.21%) and cis-vaccenic acid (14.63%), while n-hexane Soxhlet selectively enriched stigmasterol to 67.95% of total peak area approximately 19-fold higher than ethanol maceration (3.61%). Five representative metabolites, stigmasterol, phytol, lupeol, squalene, and 2-propionyl-1,4,5,6-tetrahydropyridine were subjected to molecular docking against FtsA (PDB: 3WT0), OmpF (PDB: 4KR4), and DNA gyrase B (PDB: 6KZV), with ADMET profiling performed via SwissADME and ProTox 3.0. Lupeol (-8.0 kcal/mol) and stigmasterol (-7.7 kcal/mol) surpassed the native OmpF ligand binding affinity and demonstrated strong interaction with DNA gyrase B, supporting multi-target antibacterial potential. Squalene showed preferential affinity for FtsA (-7.1 kcal/mol). Among the candidates, 2-propionyl-1,4,5,6-tetrahydropyridine was the sole Lipinski-compliant compound with high predicted oral absorption, all five metabolites displayed no predicted hepatotoxicity or carcinogenicity. These findings identify lupeol and stigmasterol as priority antibacterial leads from S. tonkinensis and establish n-hexane Soxhlet extraction as an effective strategy for targeted phytosterol enrichment from this species.
In silico Evaluation of Physicochemical and ADMET Properties of Bioactive Compounds from Marchantia paleacea. Hestia Hairima; Ghea Brigitta Dotulong; Nurul Nisa Primadiaty
JURNAL BIOSAINS Vol. 12 No. 2 (2026): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpgmn753

Abstract

  Marchantia paleacea is a liverwort with diverse secondary metabolites of pharmacological interest. This study aimed to evaluate the physicochemical and ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) properties of eighteen bioactive compounds previously identified from M. paleacea by GC-MS analysis using an in silico approach. Physicochemical parameters were analyzed using SwissADME, ADMET predictions were performed using pKCSM and ProTox 3.0 for toxicity class analysis. Results showed that most compounds complied with Lipinski’s Rule of Five (RO5), demonstrating good oral drug-likeness. All compounds exhibited high human intestinal absorption (86-95%), favorable BBB permeability, and CNS permeability values remained low. Most compounds did not significantly interact with major CYP450 isoenzymes or P-glycoprotein. Toxicity prediction revealed that the majority of compounds were non-mutagenic (Ames-negative), non-hepatotoxic, and did not inhibit the hERG channel, suggesting a relatively safe pharmacological profile. Exceptions included 2-nitro-cyclohexanone (positive Ames test) and thymol (hepatotoxicity). Compounds such as linalool,  dodecanoic acid, n-decanoic acid, and bicyclo[2.2.2]hept5-en2-ol demonstrated particularly favorable drug-like characteristics, warranting further experimental validation. These findings provide a computational foundation for prioritizing M. paleacea bioactive compounds for future drug development.   
Co-Authors Christian Yopie Daniel Siregar Siagian Dewi Indriyani Roslim Dian Wardana Dian Wardana Dwi Sapri Ramadhan Ghea Brigitta Dotulong Herman Herman Ilham Sinuraya Khory Aulia Larasati Arum Utami Nazla Suhaila Parinduri Nurul Nisa Primadiaty Rosa Nadya Evelyn Sitorus Santi Dameana Habeahan Siti Annisa Kusbama Tita Juwitaningsih Vina Octavia Azzahra Wahyu Lestari