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All Journal Jurnal Ilmu Kedokteran (Journal of Medical Science) Majalah Kedokteran Bandung Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Indonesian Journal of Biotechnology Mutiara Medika: Jurnal Kedokteran dan Kesehatan Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Majalah Kesehatan Pharmamedika Indonesian Journal of Laboratory Narra J Muhammadiyah Journal of Geriatric Indonesian Archives of Biomedical Research (InABR) Engagement: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Biomedicine and Clinical Sciences
Dwi Cahyani Ratna Sari
Departement Of Anatomy, Faculty Of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences

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Angka Kejadian Hipertensi Pada Pemetik Teh Lansia di Kemuning, Karanganyar Setiorini, Anggi; Agustiningsih, Denny; Yunus, Junaedy
Muhammadiyah Journal of Geriatric Vol 2, No 2 (2021): Muhammadiyah Journal of Geriatric
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/mujg.2.2.49-54

Abstract

Latar belakang: hipertensi mengakibatkan banyak masalah seperti penyakit kardiovaskular. Hipertensi dapat menurunkan kualitas kinerja seseorang dan mengganggu kualitas hidup. Hipertensi dapat terjadi pada semua usia tetapi paling banyak pada usia lansia karena lansia mengalami penurunan elastisitas pembuluh darah. Tujuan: tujuan penelitian ini untuk mengetahui angka kejadian dan hubungan hipertensi pada pemetik teh lansia yang berada di Perkebunan Teh Kemuning, Karanganyar meliputi umur, masa kerja, waktu kerja dan Indeks Masa Tubuh. Metode: cross sectional dengan sampel sejumlah 30 orang pemetik teh yang sudah berusia lansia, bekerja lebih dari 5 tahun, bekerja pada area kemiringan 450. Variabel yang diukur adalah hipertensi, masa kerja, waktu kerja, pendidikan, berat badan dan tinggi badan pemetik teh.Uji bivariat menggunakan Chi Square. Hasil: penelitian ini menunjukkan angka kejadian hipertensi ringan sebanyak 83,3% dan hipertensi sedang sebanyak 16,7%. Simpulan: penelitian ini adalah terdapat hubungan antara kejadian hipertensi baik ringan dan sedang terhadap umur dan masa kerja tetapi tidak terdapat hubungan antara waktu kerja dan IMT dengan kejadian hipertensi baik ringan maupun sedang karena pada sampel didapatkan bahwa IMT normal banyak. 
Cytoarchitectural changes in the developing cerebellar cortex of the laggard mutant mouse Yunus, Junaedy; Setsu, Tomiyoshi; Kikkawa, Satoshi; Terashima, Toshio
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v5i2.2075

Abstract

The laggard (lag) mutant mouse, which arises from a mutation in the Kif14 gene, begins to exhibit ataxia and impaired growth after the first postnatal week and subsequently dies prematurely around two weeks of age. In this mutant mouse, the layered architecture of the cerebellar cortex, cerebral cortex, dentate gyrus, and olfactory bulb is disrupted at the cellular level. The aim of this study was to identify the effect of Kif14 mutation on the development of the cerebellar cortex. Abnormalities in the cytoarchitectonics of the developing cerebellar cortex were assessed using hematoxylin-eosin (HE) staining and immunohistochemistry. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and bromodeoxyuridine (BrdU) assays were performed to identify apoptotic and proliferating cells. Macroscopic observation of the lag mutant cerebellum reveals a marked reduction in size compared to wild-type mice. HE staining displays a normal foliation and lamination pattern in the lag mutant cerebellum, but detailed analysis has shown morphological disorganization in the cytoarchitectonics of the cerebellar cortex. The mutant internal granular layer is poorly defined and contains significantly fewer granule cells. Meanwhile, Purkinje cells form multilayer arrangements instead of a monolayer arrangement, as observed in wild-type mice, with their dendritic arborization being severely underdeveloped. Some Purkinje cells exhibit multiple nuclei, suggesting that the Kif14 mutation disrupts normal cell division. These phenotypes are already recognized during early postnatal days, although no difference is determined before birth. TUNEL-positive cells are significantly more numerous in the mutant external granular layer, indicating that increased apoptotic cell death contributes to the diminished granule cell population in the lag mutant mouse. In conclusion, the lag mutant cerebellar cortex shows distinct structural abnormalities, suggesting that the Kif14-encoded protein exerts multifaceted roles in the development of the brain laminated structures as well as in myelin formation.
Role of nuclear factor-κB (NFκB) in microglial polarization in correlation with neuroinflammatory mechanism at the hippocampal cornu ammonis (CA) 1 region after acute and chronic phase of global ischemic brain injury in rats Wibisono, Dian Prasetyo; Nur Arfian; Fauziyatul Munawaroh; Dwi Cahyani Ratna Sari
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 3 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i3.12757

Abstract

Ischemic brain injuries can result in hippocampal injury due to its vulnerability to ischemia, specifically the CA1 region. Ischemic injury to this region alters nerve cells, synapses, and non-neural hippocampal tissue and causes hippocampal sclerosis. This injury could be mediated by microglia via the neuroinflammation pathway. However, the neuroinflammatory mechanism underlying hippocampal ischemic injury is still unclear. This study aimed to investigate the role of NF-κB in microglia polarization which affects the hippocampal area after ischemic injury. We conducted a quasi-experimental study, using 24 male Sprague Dawley rats aged 4 wk old and weighing 100 g. The rats were grouped into 4 different groups (CL1 as acute, CL3 as subacute, CL7 as chronic, and SO as control groups) and performed bilateral common carotid artery ligation to induce global ischemic injury in the brain. The difference in microglial activation was tested using immunohistochemistry for CD68. Moreover, polymerase chain reaction (PCR) was utilized to assess mRNA expression differences in IL1β, IL6, TNFα, and NF-κB. An increase in the number of positive CD68 fraction areas in CL1, CL3, and CL7 compared to the SO group (p=0.002) was shown after bilateral common carotid artery ligation. Such ligation also induced a significantly higher mRNA expression of IL1β (p=0.004), IL6 (p=0.028), TNFα (p=0.028), and NF-κB (p=0.002) in the CL1, CL3, and CL7 groups, compared to the SO group. In conclusion, NF-κB is the key player in hippocampal injury in the CA1 region following ischemic event by differentiating microglia into M1 phenotype form and initiates the neuroinflammatory cascade via IL1β, IL6, and TNFα in all phases.
Addressing Disparities in Clean and Healthy Living Behaviors through Targeted Health Education in a Rural Community Widodo, Irianiwati; Rinonce, Hanggoro Tri; Asysyifa, Azzahra; Syarifa, Cut Alima; Sabila, Aflifia Birruni; Nurrahma, Bira Arumndari; Farmawati, Arta; Sari, Dwi Cahyani Ratna; Nugrahaningsih, Dwi Aris Agung; Jaya, Sudi Indra; Sholikhah, Eti Nurwening; Rizal, Dicky Moch; Susilowati, Rina; Nuryastuti, Titik; Murhandarwati, E. Elsa Herdiana; Triyono, Teguh; Ratnaningsih, Tri
Engagement: Jurnal Pengabdian Kepada Masyarakat Vol. 10 No. 1 (2026): February 2026
Publisher : Asosiasi Dosen Pengembang Masyarajat (ADPEMAS) Forum Komunikasi Dosen Peneliti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29062/engagement.v10i1.2043

Abstract

Background: This study addresses the public health issue of community hygiene and wellness by focusing on the Clean and Healthy Lifestyle Behavior (CHLB) as a key parameter. The subject of the assessment is the community of Sompok Hamlet, Bantul, with the purpose of evaluating the implementation of CHLB within households to understand areas of strength and needed improvement. Purpose of the Study: The purpose is to assess the level of CHLB implementation in Sompok Hamlet. The objective is to measure household compliance across various CHLB indicators and identify specific behavioral gaps that require targeted intervention. Methods: The study employed a cross-sectional survey design conducted in 2023. Data were collected from 202 households across seven neighborhood units (RT) using a structured questionnaire to evaluate compliance with standard CHLB indicators. Results: The results showed that 68.3% of households met the criteria for independent healthy communities. High compliance was observed in indicators like access to clean water, sanitation, exclusive breastfeeding, and toddler weighing. However, significant gaps were identified in physical activity (71.3% low compliance) and indoor smoking practices (64% low compliance). Practices related to fruit/vegetable consumption, handwashing, and larva elimination showed varied results. The findings highlight the need for focused educational programs to address these specific behavioral deficiencies to promote sustainable community health.
Penataan Biosampel di Tangki Nitrogen Cair (LN2 tank) sebagai Dasar Pengembangan Sistem Informasi Manajemen Biosampel di Laboratorium Riset Pramatasari, F. Linda Tri; Kurniawan, Efri; Yunus, Junaedy; Fachiroh, Jajah
Indonesian Journal of Laboratory Vol 9, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijl.v9i1.110864

Abstract

ABSTRAK. Penyimpanan biosampel dalam tangki nitrogen cair merupakan metode yang efektif untuk mempertahankan integritas material biologis dalam jangka panjang. Ketertelusuran data terkait biosampel merupakan aspek penting untuk mencegah kesalahan identitas biosampel. Di sisi lain, pengelolaan biosampel di lingkungan laboratorium dengan keterbatasan infrastruktur digital memerlukan pendekatan praktis yang tetap menjamin keterlacakan dan keamanan biosampel. Tujuan penelitian ini mengembangkan sistem penataan biosampel menggunakan Microsoft Excel pada tangki nitrogen cair di Biobank Fakultas Kedokteran, Kesehatan Masyarakat, dan Keperawatan Universitas Gadjah Mada (FK-KMK UGM) sebagai solusi operasional sekaligus dasar pengembangan sistem informasi manajemen biosampel berbasis web. Metode penelitian ini mengembangkan panduan yang mencakup: penanganan tangki nitrogen cair; pencatatan data dan metadata; pengelolaan biosampel; dan uji keterlacakan. Hasil penelitian menunjukkan bahwa sistem pencatatan data dan metadata menggunakan Microsoft Excel yang diterapkan secara sistematis dan konsisten mampu meningkatkan efisiensi kerja serta manjaga keterlacakan biosampel. Pendekatan ini terbukti layak diterapkan sebagai solusi transisi bagi laboratorium dengan sumber daya terbatas sebelum implementasi sistem informasi berbasis digital secara terintegrasi.ABSTRACT. The storage of biosamples in liquid nitrogen tanks is an effective method for preserving the long-term integrity of biological materials. Data traceability associated with biosamples is a crucial aspect in preventing sample misidentification. Conversely, the management of biosamples in laboratory settings with limited digital infrastructure necessitates a practical approach that ensures both sample traceability and security. This study aims to develop a biosample organization system utilizing Microsoft Excel for liquid nitrogen tanks at the Biobank of the Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada (FK-KMK UGM). This system serves as an immediate operational solution as well as a foundational framework for the subsequent development of a web-based biosample management information system. The methodology involved the development of guidelines encompassing the handling of liquid nitrogen tanks, data and metadata documentation, biosample management, and traceability testing. The results demonstrate that a systematic and consistent application of the Microsoft Excel-based data and metadata documentation system improves operational efficiency and maintains biosample traceability. This approach proves to be a viable transitional solution for resource-constrained laboratories prior to the implementation of fully integrated, digital information systems.
Co-Authors Aflifia Birruni Sabila Amelia Amelia Anggraeni M, Andi Sri Dewi Arta Farmawati Asysyifa, Azzahra Budi Iman Santoso Daniel Agriva Tamba Denny Agustiningsih Desby Juananda Dharmansyah, Mohamad Irwan Dian Prasetyo Wibisono Dicky Moch Rizal Dicky Moch Rizal Djoko Prakosa2, Dwi Aris Agung Nugrahaningsih Dwina Agrila Lakabela E. Elsa Herdiana Murhandarwati Ery Kus Dwianingsih Eti Nurwening Sholikhah Fauziyatul Munawaroh Fauziyatul Munawaroh Ginus Partadiredja Ginus Partadiredja Hana Rutyana Putri Antonio Handoyo Pramusinto Hanggoro Tri Rinonce Hilma Kholida Ats-tsani Jajah Fachiroh, Jajah Jaya, Sudi Indra Juananda, Desby Kikkawa, Satoshi Krisnugraha, Yeshua Putra Kurniawan, Efri Lesbatta, Kezia Josawel M. Mansyur Romi Mansyur Romi Mawaddah Ar-Rochmah Muhammad Mansyur Romi Muhammad Mansyur Romi Nanang Wiyono Nur Arfian Nur Arfian Nur Arfian, Nur Nurfadilah, N Nuring Pangastuti, Nuring Nurrahma, Bira Arumndari Ova Emilia Pramatasari, F. Linda Tri Pratiwi Indah Sayekti Reza Satria Pratama Reza Satria Pratama, Reza Satria Riky Setyawan Rina Susilowati Setiorini, Anggi Setsu, Tomiyoshi Soedjono Aswin Soedjono Aswin Sri Suharmi, Sri Sumpono, Auliya Suluk Brilliant Syarifa, Cut Alima Taihuttu, Yuniasih MJ Teguh Triyono Terashima, Toshio Titik Nuryastuti Toni Febriyanto Tri Ratnaningsih Wibisono, Dian Prasetyo Widodo, Irianiwati Wiwit Ananda Wahyu Setyaningsih Yenni Zulhamidah, Yenni Yeshua Putra Krisnugraha Yuniartha, Ratih