Article Per Year (5 Year)
p-Index From 2021 - 2026
1.182
P-Index
This Author published in this journals
All Journal Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Biosense Rumphius Pattimura Biological Journal Kalwedo Sains (KASA) JSSF : Journal of Sport Science and Fitness
Lesbatta, Kezia Josawel
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health Other

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Efek Antidiabetes Yoghurt Pala Terhadap Penurunan Kadar Glukosa Darah Tikus Model Diabetes Melitus Tipe 2 Unitly, Adrien Jems Akiles; Baszary, Chomsa Dintasari Umi; Moniharapon, Debby D.; Watuguly, Theopilus W.; Moniharapon, Mechiavel; Eddy, La; Lesbatta, Kezia Josawel; Sillehu, Eka Safitri; Sikafir, Beatrix Belina; Sopacua, Gracia N.
Kalwedo Sains (KASA) Vol 5 No 2 (2024): Kalwedo Sains (KASA), September 2024
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav5i2p91-98

Abstract

Diabetes Melitus tipe 2 adalah penyakit gangguan metabolik yang di tandai oleh kenaikan gula darah akibat penurunan sekresi insulin oleh sel beta pankreas atau gangguan fungsi insulin (resistensi insulin). Potensi yoghurt dalam menurunkan kadar glukosa darah berhubungan dengan kemampuan bakteri asam laktat dalam yoghurt menurunkan kadar kolesterol dan plasma lipid, sehingga meningkatkan sensitivitas insulin pankreas. Buah pala mengandung senyawa kimia yang dapat menunjukkan sifat antioksidan dan antibakteri. Tujuan penelitian ini untuk mengetahui efek terapi yoghurt pala terhadap penurunan kadar glukosa darah tikus model diabetes melitus tipe 2. Penelitian ini menggunakan rancangan acak lengkap (RAL). Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari lima perlakuan dan tiga kali ulangan, yaitu K(-): Kelompok kontrol negatif yaitu kelompok tikus yang diberi STZ, K(+): Kelompok kontrol positif yaitu kelompok tikus yang diberi STZ 45 mg/ekor/hari kemudian diberikan obat glibenklamid selama 14 hari, 1.25: Kelompok tikus yang diberi STZ 45 mg /ekor/hari kemudian diberi ekstrak yoghurt pala 1.25 ml /ekor/hari selama 14 hari, 2.5: Kelompok tikus yang diberi STZ 45 mg /ekor/hari kemudian diberi ekstrak yoghurt pala 2.5 ml/ekor/hari selama 14 hari dan 5.0: Kelompok tikus yang diberi STZ 45 mg /ekor/hari kemudian diberi ekstrak yoghurt pala 5 ml/ekor/hari selama 14 hari. Hasil penelitian menunjukkan pemberian yoghurt pala terbukti memiliki efek antioksidan dalam menurunkan kadar glukosa darah dimana dosis yoghurt pala yang efektif adalah 5.0 ml.
Evaluation of Analgesic Activity Corypalmine and Panasenoside Compounds on Cox-2 Receptors In-Silico Lesbatta, Kezia Josawel; Watuguly, Theopilus Wilhelmus; Kakisina, Alter Glen; Anggraeni, Andi Sri Dewi
RUMPHIUS Vol 7 No 1 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i1p031-037

Abstract

Pain is a mechanism that occurs due to stimulation of nociceptors as pain receptors that cause ongoing or future tissue damage. Analgesic drugs that are usually used are COX-2 inhibitor drugs that can inhibit the activity of the COX-2 enzyme in synthesizing Prostaglandins (PG) which respond to pain and inflammation. Specific research on active compounds such as corypalmine and panasenoside related to their effects as analgesics has not been widely carried out, so it is necessary to predict the effects of both compounds by analyzing their activity on the COX-2 protein through in silico docking studies. This study aims to see the analgesic activity between the two compounds as COX-2 inhibitors. Testing was carried out through the stages of protein and ligand preparation, method validation, molecular docking of corypalmine and panasenoside compounds with COX-2 and visualization of bonds using Ligplot+. The results showed that the corypalmine docking score was more negative than panasenoside on COX-2, respectively -60.33 and -42.02. It can be concluded that the corypalmine compound is able to bind more strongly to COOX-2 and predicted to have a strong and more stable analgesic effect than panacea.
Pemberdayaan Kelompok Petani Argowisata Banda Lonthoir Melalui Pelatihan Pembuatan Eco-enzyme Berbahan Dasar Limbah Kulit Pala Yunita, Melda; Astuti, Eka; Patteilohy, Merry; Lesbatta, Kezia Josawel; Warella, Juen Carla
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. 2 (2025): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.11.2.266-275

Abstract

Organic waste is a major environmental and societal concern. This waste originates from households, agricultural industries, and livestock. Piles of organic waste that are not managed properly will cause water, soil, and air pollution. Banda Lonthoir Village is one of the areas that has not implemented proper processing and utilization of organic waste. This is because Banda Lonthoir Village is the largest producer of nutmeg in Maluku. The nutmeg plants that are utilized are seeds and mace, whereas nutmeg skin is usually made into sweets, and most of it is thrown away as organic waste. One solution for processing organic waste from nutmeg skin is to use eco-enzymes. This activity aims to provide education and training in creating eco-enzymes to the community so that they can increase their knowledge on how to manage nutmeg skin waste. The method of implementing this activity is to provide materials and training processes for making eco-enzymes. The target of this activity is the Nutmeg Garden Agrotourism Farmers Group and the Banda Lonthoir Village apparatus. The results of this activity show that the community is very enthusiastic and participates in listening to the material and making eco-enzymes, as well as asking questions related to the activities provided. The community was able to make eco-enzymes according to the procedure explained and ferment 10 L of eco-enzyme. The community gave a positive response and had an impact on increasing community knowledge regarding how to manage nutmeg skin waste into eco-enzymes that are beneficial for the community and the environment.
Physical Exercise And Epigenetic: Molecular Mechanisms Anggraeni. M, Andi Sri Dewi; Arimbi; A.Anggriani; Lesbatta, Kezia Josawel; Indriani
Journal of Sport Science and Fitness Vol. 11 No. 2 (2025): JSSF: Journal of Sport Science and Fitness
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jssf.v11i2.34037

Abstract

Introduction: Physical exercise is associated with an active lifestyle and offers numerous health benefits. Recent research has shown that physical exercise also influences gene expression through epigenetic mechanisms such as DNA methylation, histone modification, mRNA, and microRNA expression. These changes contribute to physiological changes and disease prevention. Objectives: This review aims to explain how physical activity can affect a person's epigenetic changes to have an impact on the prevention of chronic diseases. Method: This study is a literature review using databases such as PubMed from 2015 to 2025. Result: the review revealed that physical activity can cause epigenetic changes at the stage of DNA methylation, histone modification, RNA methylation and MiRNA. Physical activity modulates DNA methylation, affecting genes related to metabolism and immune function. Understanding this pathway is the basis for developing personalized exercise programs as interventions for the prevention and therapy of chronic diseases. Understanding this pathway is the basis for developing personalized exercise programs as interventions for the prevention and therapy of diseases. Conclusion: This study reveals the relevance of epigenetic changes to physical activity as an alternative non-pharmacological therapy because it is accompanied by a perspective in choosing the type of physical activity that suits the body's condition as a step in implementing a more precise health pattern.
UJI IN SILLICO ANTIBAKTERI SENYAWA AKTIF KUERSETIN DAUN BINAHONG TERHADAP PROTEIN ClfB Staphylococcus aureus Lesbatta, Kezia Josawel; Umanailo, Annisa R.; Lumintang, Vallery I. A.; Lermatan, Marta Dominika; Kuay, Yonna A.; Aponno, Novellis V.; Anggraeni. M, Andi Sri Dewi
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.7097

Abstract

Abstrak Resistensi antibiotik terhadap Staphylococcus aureus, khususnya strain Methicillin-Resistant Staphylococcus aureus (MRSA), merupakan tantangan besar dalam pengobatan infeksi bakteri. Salah satu protein virulensi penting dari S. aureus adalah Clumping factor B (ClfB), yang berperan dalam proses adhesi dan pembentukan biofilm pada jaringan inang. Penghambatan protein ini dapat menurunkan kemampuan kolonisasi bakteri, sehingga menjadi target potensial dalam pengembangan agen antibakteri baru. Penelitian ini bertujuan untuk mengevaluasi potensi kuersetin dari daun binahong (Anredera cordifolia) sebagai inhibitor alami terhadap protein ClfB secara in silico. Struktur tiga dimensi protein ClfB diperoleh dari Protein Data Bank (PDB), sedangkan struktur kuersetin diunduh dari basis data PubChem. Proses molecular docking dilakukan menggunakan SwissDock dengan analisis lanjutan melalui UCSF Chimera. Hasil docking menunjukkan bahwa kuersetin berikatan kuat pada kantong aktif ClfB dengan nilai afinitas energi sebesar −7,584 kcal/mol, menandakan kestabilan kompleks yang baik secara termodinamika. Interaksi yang terbentuk melibatkan residu asam amino polar seperti Ser, Glu, dan Asp serta residu nonpolar seperti Leu dan Val yang berkontribusi terhadap pengikatan stabil. Visualisasi struktur menunjukkan bahwa kuersetin menempati sisi aktif ClfB dan berpotensi menghambat fungsi adhesi protein tersebut. Berdasarkan hasil ini, kuersetin dari daun binahong berpotensi menjadi kandidat inhibitor alami ClfB yang dapat mengganggu proses kolonisasi S. aureus pada jaringan inang. Penelitian ini memberikan dasar ilmiah bagi pengembangan agen antibakteri berbasis bahan alam dalam upaya mengatasi resistensi antibiotik. Kata kunci: Staphylococcus aureus; ClfB; kuersetin; binahong; antibakteri. Abstrack Antibiotic resistance in Staphylococcus aureus, particularly Methicillin-Resistant Staphylococcus aureus (MRSA), poses a serious challenge to the treatment of bacterial infections worldwide. Clumping factor B (ClfB) is an important virulence protein of S. aureus that plays a crucial role in bacterial adhesion and biofilm formation on host tissues. Inhibition of this protein may reduce bacterial colonization and represents a promising target for novel antibacterial strategies. This study aimed to evaluate the potential of quercetin, an active compound derived from binahong leaves (Anredera cordifolia), as a natural inhibitor of the ClfB protein using an in silico approach. The three-dimensional structure of ClfB was obtained from the Protein Data Bank, while the molecular structure of quercetin was retrieved from the PubChem database. Molecular docking was performed using SwissDock, and the interaction analysis and visualization were conducted using UCSF Chimera. The docking results showed that quercetin exhibited a strong binding affinity toward the active site of ClfB, with a binding energy of −7.584 kcal/mol, indicating favorable thermodynamic stability of the ligand–protein complex. Several polar amino acid residues, including serine, glutamate, and aspartate, as well as nonpolar residues such as leucine and valine, were involved in stabilizing the interaction. Structural visualization confirmed that quercetin occupies the active pocket of ClfB, suggesting its ability to interfere with bacterial adhesion mechanisms. Overall, quercetin from binahong leaves demonstrates potential as a natural ClfB inhibitor and provides a scientific basis for the development of alternative antibacterial agents to address antibiotic resistance in Staphylococcus aureus and support future antimicrobial research. Keywords: Staphylococcus aureus; ClfB; quercetin; binahong; antibacterial.
Co-Authors A.Anggriani Adrien Jems Akiles Unitly Anggraeni M, Andi Sri Dewi Anggraeni, Andi Sri Dewi Anggraeni. M, Andi Sri Dewi Aponno, Novellis V. Arimbi ASTUTI, EKA Baszary, Chomsa Dintasari Umi Dharmansyah, Mohamad Irwan Dwi Cahyani Ratna Sari Eka Safitri Sillehu Indriani Kakisina, Alter Glen Kuay, Yonna A. La Eddy Lermatan, Marta Dominika Lumintang, Vallery I. A. Melda Yunita Moniharapon, Debby D. Moniharapon, Mechiavel Nungki Anggorowati Nur Arfian, Nur Nurfadilah, N Patteilohy, Merry Sikafir, Beatrix Belina Sopacua, Gracia N. Theopilus W Watuguly Umanailo, Annisa R. Warella, Juen Carla Yuniartha, Ratih