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All Journal VALENSI Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan POSITRON Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology Jurnal Kelautan Tropis Journal Of Chemical Process Engineering (JCPE) ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Pure and Applied Chemistry
Winda Rahmalia
1. Jurusan Kimia, Fakultas MIPA, Universitas Tanjungpura 2. Chemistry Insight Center Fakultas MIPA, Universitas Tanjungpura
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Energy Engineering Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Physics

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Extraction and Characterization of Astaxanthin from Processed Rebon Shrimp Products as a Local Wisdom-Based Antioxidant Source Delia Ramadhina Tanzikha; Anis Shofiyani; Winda Rahmalia; Dwi Imam Prayitno; Intan Syahbanu; Syarifah Jubaidah; Titik Lestari
Jurnal Kelautan Tropis Vol 29, No 2 (2026): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v29i2.31340

Abstract

Astaxanthin, a carotenoid with pronounced antioxidant properties, represents a promising candidate for development as a functional food ingredient grounded in local wisdom. Traditional rebon shrimp derivatives including dried rebon, terasi, and cincalok are recognized sources of this pigment; however, systematic data on extraction efficiency, astaxanthin concentration, and antioxidant activity remain scarce. This study evaluated three extraction techniques and characterized antioxidant activity using 2,2-difenil-1-pikrilhidrazil (DPPH), azinobis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), and 4,6-tris(2-pyridyl)-s-triazine (FRAP) assays. Samples were vacuum-dried, ground, and subjected to oil extraction, followed by qualitative thin-layer chromatography and quantitative UV-Vis spectrophotometry. Soxhlet extraction yielded the greatest oil output, whereas maceration preserved astaxanthin more effectively due to the absence of thermal degradation. Cincalok exhibited the highest astaxanthin content (10.943 mg/g), followed by terasi and dried rebon. DPPH analysis indicated that cincalok oil possessed the strongest antioxidant activity (IC₅₀ 54.58 mg/L, Soxhlet), while dried rebon demonstrated the weakest. In contrast, ABTS and FRAP assays revealed lower responses for cincalok oil, suggesting that its primary antioxidant mechanism involves free-radical scavenging. These findings highlight cincalok as the most promising natural source of astaxanthin and local antioxidants, underscoring its potential for the development of coastal-based functional foods and health-promoting products.
Formulasi dan Uji Aktivitas Krim Pengkelat Merkuri Berbahan Dasar Ekstrak Etanol Alga Coklat (Sargassum sp.) Inda Putrinesia; Yoga Pratama; Nurul Asyikin; Winda Rahmalia
ALCHEMY Jurnal Penelitian Kimia Vol 14, No 1 (2018): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.14.1.12242.152-163

Abstract

Telah dilakukan penelitian tentang formulasi dan uji aktivitas krim pengkelat merkuri berbahan dasar ekstrak etanol alga coklat (Sargassum sp.) telah dilakukan. Krim diformulasikan dengan beberapa bahan seperti vaselin, gliserol, asam askorbat, karbomer, setil alkohol, dan NaOH. Parameter yang dievaluasi diantaranya organoleptik krim, homogenitas, pH, daya sebar, tipe krim, iritasi kulit, dan daya tercuci krim. Aktivitas pengkelatan diamati dengan menganalisis kandungan merkuri pada kulit tikus yang sebelumnya diberikan krim bermerkuri dibandingkan dengan kulit tikus yang telah dioleskan krim pengkelat merkuri berbahan dasar ekstrak etanol alga coklat. Spektrum Fourier Transform Infrared Spectroscopy (FT-IR) menunjukkan bahwa ekstrak etanol alga coklat diindikasikan mengandung glutation. Berdasarkan parameter evaluasi krim, formulasi terbaik adalah krim dengan komposisi ekstrak alga coklat sebanyak 0,035% w/v terhadap volume krim, yang ditandai dengan krim yang berbentuk gel kental dan tidak berbau serta memiliki tekstur seperti krim wajah pada umumnya, yaitu lembut dan sedikit berminyak. Krim bersifat homogen dan stabil hingga hari ke 21 percobaan dengan pH=5. Krim juga tidak menimbulkan iritasi dan dapat tercuci habis tanpa bekas. Hasil analisis AAS membuktikan bahwa penggunaan krim pengkelat merkuri berbahan dasar esktrak etanol alga coklat selama kurang dari 2 minggu dengan dosis pemberian 1 kali sehari mampu menyerap merkuri pada tikus dengan aktivitas penyerapan sebesar 50%.Formulation and Activity Test of Mercury Chelator Cream of Ethanol Extract of Brown Algae (Sargassum Sp.). Research on the study of formulation an activity test of mercury chelator cream from ethanol extract has successfully done. The cream was formulated with ingredients such as vaseline, glycerol, ascorbic acid, carbomer, cetyl alcohol, and sodium hydroxide. Parameters were evaluated against organoleptic, homogenity, pH, dispersion type of cream, skin irritation test and washing test. The mercury chelating activity was observed by analyzing mercury concentration in mercury induced mouse skin. The FTIR spectrum shows that ethanol extract of brown algae was dominated by glutathione functional group. The best cream formulation was composed by 0,035% (w/v) brown algae, formed as a creamy gel that was thick and odorless and had a texture like soft facial cream in general with slightly oily.The cream was homogeneous and stable until the 21st day of the experiment with pH = 5. The cream produced no skin irritation and could be washed out without a trace. The results of AAS analysis proved that the use of brown algae ethanol extract-based mercury chelating cream for less than 2 weeks with a dose of 1 time a day could absorb mercury in mouse skin with absorption activity of 50%.
Co-Authors Adhitiyawarman, Adhitiyawarman Angie Islammiyati Anis Shofiyani Annisa Bella Maharani Ayu Widya Sari Delia Ramadhina Tanzikha Deni Pranata Dwi I. Prayitno Dwi Imam Prayitno Esi Amelyanti Fajar Nugraha Fajriaty, Inarah Gusrizal Gusrizal Hadi Kurniawan Hairunnisa Hairunnisa Hamdil Mukhlishin Imelda Hotmarisi Silalahi Inda Putrinesia Intan Syahbanu Intan Syahbanu Intan Syahbanu Intan Syahbanu Lia Destiarti M Syahwandi Mas Inda Putrinesia Mauludia Mauludia Nia Dahlia Nico Gustian Nimas Ade Kusdayanti Nur Ridha An Nisa Nurlina Nurlina Nurlina Nurlina, Nurlina Nurul Asyikin Prayitno, Dwi Imam Puji Ardiningsih Ramadhy, Wahyu Febri Risya Sasri Sabrina Maulidya Amir Septiani Septiani Septiani Septiani Septiani Siti Nani Nurbaeti Syarifah Jubaidah Thamrin Usman Thamrin Usman Titik Lestari Titin Anita Zahara Titin Anita Zahara Titin Anita Zaharah Uray Amira Naselia Uray Amira Naselia Utin Widiatannur Wahyu Febri Ramadhy Yoga Pratama Zulenda Zulenda