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Extraction and Characterization of Astaxanthin from Processed Rebon Shrimp Products as a Local Wisdom-Based Antioxidant Source Delia Ramadhina Tanzikha; Anis Shofiyani; Winda Rahmalia; Dwi Imam Prayitno; Intan Syahbanu; Syarifah Jubaidah; Titik Lestari
Jurnal Kelautan Tropis Vol 29, No 2 (2026): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v29i2.31340

Abstract

Astaxanthin, a carotenoid with pronounced antioxidant properties, represents a promising candidate for development as a functional food ingredient grounded in local wisdom. Traditional rebon shrimp derivatives including dried rebon, terasi, and cincalok are recognized sources of this pigment; however, systematic data on extraction efficiency, astaxanthin concentration, and antioxidant activity remain scarce. This study evaluated three extraction techniques and characterized antioxidant activity using 2,2-difenil-1-pikrilhidrazil (DPPH), azinobis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), and 4,6-tris(2-pyridyl)-s-triazine (FRAP) assays. Samples were vacuum-dried, ground, and subjected to oil extraction, followed by qualitative thin-layer chromatography and quantitative UV-Vis spectrophotometry. Soxhlet extraction yielded the greatest oil output, whereas maceration preserved astaxanthin more effectively due to the absence of thermal degradation. Cincalok exhibited the highest astaxanthin content (10.943 mg/g), followed by terasi and dried rebon. DPPH analysis indicated that cincalok oil possessed the strongest antioxidant activity (IC₅₀ 54.58 mg/L, Soxhlet), while dried rebon demonstrated the weakest. In contrast, ABTS and FRAP assays revealed lower responses for cincalok oil, suggesting that its primary antioxidant mechanism involves free-radical scavenging. These findings highlight cincalok as the most promising natural source of astaxanthin and local antioxidants, underscoring its potential for the development of coastal-based functional foods and health-promoting products.
Formulasi dan Uji Aktivitas Krim Pengkelat Merkuri Berbahan Dasar Ekstrak Etanol Alga Coklat (Sargassum sp.) Inda Putrinesia; Yoga Pratama; Nurul Asyikin; Winda Rahmalia
ALCHEMY Jurnal Penelitian Kimia Vol 14, No 1 (2018): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.14.1.12242.152-163

Abstract

Telah dilakukan penelitian tentang formulasi dan uji aktivitas krim pengkelat merkuri berbahan dasar ekstrak etanol alga coklat (Sargassum sp.) telah dilakukan. Krim diformulasikan dengan beberapa bahan seperti vaselin, gliserol, asam askorbat, karbomer, setil alkohol, dan NaOH. Parameter yang dievaluasi diantaranya organoleptik krim, homogenitas, pH, daya sebar, tipe krim, iritasi kulit, dan daya tercuci krim. Aktivitas pengkelatan diamati dengan menganalisis kandungan merkuri pada kulit tikus yang sebelumnya diberikan krim bermerkuri dibandingkan dengan kulit tikus yang telah dioleskan krim pengkelat merkuri berbahan dasar ekstrak etanol alga coklat. Spektrum Fourier Transform Infrared Spectroscopy (FT-IR) menunjukkan bahwa ekstrak etanol alga coklat diindikasikan mengandung glutation. Berdasarkan parameter evaluasi krim, formulasi terbaik adalah krim dengan komposisi ekstrak alga coklat sebanyak 0,035% w/v terhadap volume krim, yang ditandai dengan krim yang berbentuk gel kental dan tidak berbau serta memiliki tekstur seperti krim wajah pada umumnya, yaitu lembut dan sedikit berminyak. Krim bersifat homogen dan stabil hingga hari ke 21 percobaan dengan pH=5. Krim juga tidak menimbulkan iritasi dan dapat tercuci habis tanpa bekas. Hasil analisis AAS membuktikan bahwa penggunaan krim pengkelat merkuri berbahan dasar esktrak etanol alga coklat selama kurang dari 2 minggu dengan dosis pemberian 1 kali sehari mampu menyerap merkuri pada tikus dengan aktivitas penyerapan sebesar 50%.Formulation and Activity Test of Mercury Chelator Cream of Ethanol Extract of Brown Algae (Sargassum Sp.). Research on the study of formulation an activity test of mercury chelator cream from ethanol extract has successfully done. The cream was formulated with ingredients such as vaseline, glycerol, ascorbic acid, carbomer, cetyl alcohol, and sodium hydroxide. Parameters were evaluated against organoleptic, homogenity, pH, dispersion type of cream, skin irritation test and washing test. The mercury chelating activity was observed by analyzing mercury concentration in mercury induced mouse skin. The FTIR spectrum shows that ethanol extract of brown algae was dominated by glutathione functional group. The best cream formulation was composed by 0,035% (w/v) brown algae, formed as a creamy gel that was thick and odorless and had a texture like soft facial cream in general with slightly oily.The cream was homogeneous and stable until the 21st day of the experiment with pH = 5. The cream produced no skin irritation and could be washed out without a trace. The results of AAS analysis proved that the use of brown algae ethanol extract-based mercury chelating cream for less than 2 weeks with a dose of 1 time a day could absorb mercury in mouse skin with absorption activity of 50%.
Pembuatan lilin dari minyak jelantah: pelatihan peningkatan keterampilan dan nilai tambah produk rumah tangga bagi ibu rumah tangga di Kecamatan Entikong Kalimantan Barat Risya Sasri; Winda Rahmalia; Lia Destiarti; Adhitiyawarman Adhitiyawarman; Puji Ardiningsih; Afghani Jayuska; Berlian Sitorus; Anthoni B. Aritonang; Firman Shantya Budi; Nurlina Nurlina; Elliska Murni Harfinda; Ferdinand Hidayat
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 10, No 3 (2026): June
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v10i3.38992

Abstract

Abstrak Program Pengabdian kepada Masyarakat ini dilakukan dengan tujuan untuk memberdayakan ibu rumah tangga di Kecamatan Entikong melalui pelatihan keterampilan pengolahan minyak jelantah menjadi lilin. Kegiatan ini menggabungkan aspek edukasi lingkungan, peningkatan keterampilan praktis, serta pembukaan peluang ekonomi skala rumah tangga. Dengan pendekatan partisipatif dan praktik langsung, diharapkan peserta tidak hanya mendapatkan pengetahuan baru, tetapi juga mampu mereplikasi proses pembuatan lilin secara mandiri. Pelaksanaan kegiatan PKM dilakukan dalam beberapa tahap antara lain, persiapan, pelaksanaan dan evaluasi program PKM. Tahap persiapan meliputi orientasi lapangan dalam rangka mengidentifikasi masalah mitra dan kesepakatan teknologi yang akan diimplementasikan, perijinan dan sosialisasi.  Dilanjutkan dengan tahap pelaksanaan yang meliputi Pelatihan Pembuatan Lilin diawali dengan 1).Pengenalan bahan tambahan (asam stearat , pewarna, aromaterapi opsional); 2). Praktik pembuatan lilin dari minyak jelantah; 3). Pencetakan dan pendinginan lilin; 4). Tips keamanan dan penyimpanan. Pada tahap akhir dilakukan evaluasi secara kuantitatif dengan menganalisis kuesioner pengetahuan awal dan kuesioner evaluasi lanjutan. Dibuat bukti tertulis terkait keberhasilan pelaksanaan program. Kegiatan ini merupakan bentuk kontribusi Jurusan Kimia FMIPA Universitas Tanjungpura dalam mendukung pengelolaan limbah yang bertanggung jawab, pemberdayaan perempuan, serta pembangunan berkelanjutan di wilayah perbatasan. Kata kunci: lilin; minyak jelantah; perbatasan; pengabdian; pelatihan. Abstract This Community Service Program aims to empower homemakers in Entikong District through training in processing used cooking oil into candles. This activity combines aspects of environmental education, improving practical skills, and opening up economic opportunities on a household scale. With a participatory approach and direct practice, it is hoped that participants will not only gain new knowledge but also be able to replicate the candle-making process independently. The implementation of the PKM activity is carried out in several stages, including preparation, implementation, and evaluation of the PKM program. The preparation stage includes fieldwork in order to identify partner problems and technological agreements to be implemented, licensing, and socialization, and is continued with the implementation stage, which includes Candle Making Training, starting with 1). Introduction to additional materials (stearin, dyes, optional aromatherapy); 2). Practice making candles from used cooking oil; 3). Molding and cooling candles; 4). Safety and storage tips. In the final stage, a quantitative evaluation is conducted by analyzing the results of the pretest and posttest, as well as the questionnaire responses before and after the activity is implemented. Written evidence is made regarding the success of the program implementation. This activity is a contribution from the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Tanjungpura, in support of responsible waste management, women's empowerment, and sustainable development in border areas. Keywords: candles; used cooking oil; borders; community service; training.
Synthesis of Nd-Pheophytin Complex and Its Hydrolysis into Nd-Pheophorbide from Katuk Leaves (Sauropus androgynous (L.) Merr) Fadia Novayanti; Adhitiyawarman Adhitiyawarman; winda rahmalia
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 2 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 2, Desember 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.112.02

Abstract

Pheophorbide, as a chlorophyll derivative, has potential as a complexing ligand for heavy metals, including lanthanides. The synthesis of Nd-pheophytin complex and its more polar Nd-pheophorbide (pheophytin’s hydrolyzed product) were investigated using pheophytin isolated from Katuk’s (Sauropus androgynus L. Merr.) leaves. This study aims to determine the optimal reaction conditions and the complex characteristics. Pheophytin ligand and Nd were complexed under two different reaction conditions: room temperature and reflux at 65 °C for 10 hours. The pheophytin starting material was obtained by preparative thin-layer chromatography (PTLC), yielding 0.02 g. UV-vis analysis revealed characteristic absorption bands at 404 nm (Soret band) and 666 nm (Q band) in methanol, while FTIR spectra confirmed the presence of functional groups corresponding to pheophytin. The Nd-pheophytin complex formation was indicated by hypsochromic shifts in UV-vis spectra, suggesting the Nd³⁺ complexing. Fluorescence spectra between pheophytin and Nd-pheophytin showed distinct emission patterns, with pheophytin exhibiting peaks at 662 and 722 nm, while Nd-pheophytin displayed peaks with shoulders at 654 and 714 nm. Hydrolysis of Nd-pheophytin using 1 M NaOH at pH 10 produced Nd-pheophorbide. TLC analysis showed a decrease in the Rf value of Nd-pheophytin to Nd-pheophorbide from 0.95 to 0.43, with tailing, attributed to the higher polarity of pheophorbide. These findings confirm the successful synthesis and hydrolysis of the Nd-pheophytin complex.