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Potensi Bakteri Endofit dari Tanaman Jahe Sebagai Agens Pengandali Hayati Kristianingrum, Sabrina Ayu; Setiawan, Andree Wijaya; Jayanti, Ruth Meike
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12263

Abstract

The use of synthetic pesticides is not effective in reducing the prevalence of disease because it produces residues and does not support biodiversity. This is an unwise practice and can cause problems in the future.  The use of endophytic microorganisms is widely used to control plant diseases and pests, because it is known to have various pathogen inhibition mechanisms. Biological agents that are used to inhibit plant pathogens and have been widely developed, one of which is endophytic bacteria. The purpose of the research was to evaluate the potential of endophytic bacterial isolate from ginger plants as biological control agents. The type of research used is descriptive and exploratory. Sampling of healthy ginger plants was carried out at 5 points in two sub-districts, namely Getasan and Sumowono, Semarang Regency. Isolation is carried out by taking healthy ginger plants from the roots, rhizomes, fronds, and leaves. Characterization and selection of isolates were carried out by testing their phosphate dissolving ability, nitrogen anchoring, amylase and protease activities, and antagonist tests with Fusarium oxysporum in vitro. The results of this study are that (1) 20 pure isolates were successfully isolated consisting of 2 isolates derived from leaves, 4 isolates from fronds, 8 isolates from roots, and 6 isolates from rhizomes; (2) D91 isolate has the potential as a biological agent with an antagonist mechanism of 86.91% supported by protease enzyme activity, able to dissolve phosphate and anchor nitrogen.
Identification and Characterization of Ralstonia solanacearum Species Complex from Ginger (Zingiber officinale) in Semarang Regency, Indonesia Setiawan, Andree Wijaya; Jayanti, Ruth Meike; Herawati, Maria Marina; Natalia, Kezia; Kusuma, Jonathan Galih Raka
Agro Bali : Agricultural Journal Vol 7, No 2 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i2.1731

Abstract

The Ralstonia solanacearum species complex is a highly destructive plant pathogen with a remarkably broad range of hosts, and ongoing discoveries continue to expand its host list. In May 2023, a new type of bacterial wilt affecting ginger (Z. officinale) crops in Semarang Regency, Central Java was reported. Early symptoms included sudden withering of leaves in adult plants followed by complete wilting and darkening of the vasculature, ultimately leading to plant death. This research specifically investigates the spread of Ralstonia solanacearum Species Complex within ginger cultivation in Semarang Regency. Twenty bacterial isolates were collected from soil and diseased Z. officinale plants at twenty different locations. Physiological and biochemical analyses confirmed that the causative agent for Z. officinale bacterial wilt was Ralstonia solanacearum belonging to biovar 3 and 4. The study also revealed that the distribution of this pathogen remains focused in the Banyubiru and Sumowono sub-districts. These findings will enhance our understanding of how Ralstonia solanacearum Species Complex spreads among ginger crops and its impact on them.
PENGARUH PENAMBAHAN CANGKANG TELUR PUYUH TERHADAP PERTUMBUHAN DAN HASIL MICROGREENS TANAMAN SELADA (Lactuca sativa L) Natajaya, Prasetyo; Andree Wijaya Setiawan
Journal of Innovation Research and Knowledge Vol. 4 No. 4: September 2024
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jirk.v4i4.8540

Abstract

Peningkatan jumlah penduduk di wilayah perkotaan meningkatkan permintaan untuk pasokan makanan bergizi secara berkelanjutan. Penelitian oleh Pinto et al. (2015) menunjukkan bahwa microgreens selada, meskipun memiliki kandungan nitrat lebih rendah dibandingkan tanaman dewasa, mengandung mineral seperti kalsium, magnesium, besi, mangan, seng, selenium, dan molibdenum dalam kadar yang lebih tinggi. Dengan meningkatnya limbah cangkang telur puyuh akibat konsumsi telur puyuh yang meningkat 16,4% di Indonesia (Susenas, 2016), perlu dilakukan daur ulang limbah tersebut. Penelitian ini bertujuan untuk menilai pengaruh penambahan cangkang telur puyuh terhadap pertumbuhan dan hasil microgreens selada serta menentukan dosis terbaik untuk hasil optimal. Menggunakan metode Rancangan Acak Kelompok (RAK), penelitian ini melibatkan lima perlakuan (kontrol dan dosis cangkang telur puyuh 10g, 20g, 30g, 40g) yang diulang lima kali, dengan 25 sampel. Parameter yang diamati termasuk tinggi tanaman, klorofil, karotenoid, dan vitamin C. Hasilnya menunjukkan bahwa penambahan cangkang telur puyuh mempengaruhi tinggi tanaman, klorofil, dan karotenoid. Perlakuan kontrol menghasilkan tinggi tanaman terbaik (6,998 cm), sedangkan penambahan 40 gram cangkang telur puyuh memberikan hasil terbaik untuk klorofil dan karotenoid dengan nilai 3,341; 1,169; dan 0,997.
Morphological and biochemical identification of Ralstonia solanacearum strains in ginger (Zingiber officinale Roscoe) wilt disease Natalia, Kezia; Setiawan, Andree Wijaya; Jayanti, Ruth Meike
Jurnal Ilmiah Pertanian Vol. 21 No. 2 (2024): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v21i2.19616

Abstract

The Ralstonia solanacearum Species Complex (RSSC) is a significant plant pathogen affecting various agricultural commodities, including ginger. Understanding the distribution and characteristics of RSSC in ginger plants is crucial for effective disease management. This study aims to determine the distribution of RSSC in ginger plants and identify the pathogen through morphological and biochemical characterization. The research was conducted in Banyubiru and Getasan districts, Semarang Regency, Indonesia. The study involved purposive sampling, isolation, and purification of isolates, followed by morphological characterization through observation and biochemical characterization using Gram staining with KOH, oxidase test, arginine activity test, hypersensitivity test, bacterial density calculation, Koch's postulates, and biovar characterization. Three isolates from Banyubiru displayed typical morphological characteristics of RSSC, including round, mucoid colonies with red centers and white edges on TZC medium, and rod-shaped bacterial cells. Biochemical characterization identified these isolates as RSSC strains biovar 3 and 4, capable of causing bacterial wilt in ginger plants. The study confirmed the presence of bacterial wilt in ginger in Banyubiru district. The findings reveal the spread of bacterial wilt caused by RSSC in ginger plants in Banyubiru, Semarang Regency. There is a need for measures to control the potential spread of RSSC in the surrounding host plant areas in Tlumpak Village, Banyubiru district.
Efficacy of neem and basil leaf extracts in controlling whitefly (Bemisia Tabaci) infestations and enhancing yield in curly red chili peppers (Capsicum annuum L.) Diputra, Moses Graciano; Setiawan, Andree Wijaya
Jurnal Ilmiah Pertanian Vol. 21 No. 2 (2024): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v21i2.19776

Abstract

Whiteflies (Bemisia tabaci) are a major pest of curly red chili peppers, acting as vectors for gemini viruses that significantly reduce crop productivity. This study evaluated the efficacy of neem and basil leaf extracts at three different concentrations for controlling whitefly infestations on chili plants. A randomized complete block design (RCBD) was employed with 8 treatments and 2 controls: PA (Neem 1%), PB (Neem 2%), PC (Neem 3%), PD (Basil 1%), PE (Basil 2%), PF (Basil 3%), PKM (commercial pesticide as positive control), and P0 (distilled water as negative control). Parameters assessed included whitefly infestation intensity, yellowing of shoots, fruit weight, total fruit count, and the number of infested fruits. The results indicate that both neem and basil leaf extracts effectively reduced whitefly infestation intensity and yellowing of shoots compared to the negative control (P0). Despite some increase in infestation intensity in subsequent observations, both neem and basil extracts positively impacted fruit yield, including higher fruit weight and total fruit count. However, there was no significant effect on the number of infested fruits with the treatments. These findings suggest that neem and basil leaf extracts are promising alternatives for managing whitefly infestations and improving yield in curly red chili peppers.
Simpanan Karbon Tanah pada Lahan Sawah Irigasi, Sawah Tadah Hujan, dan Tegalan di Kecamatan SUB-DAS Tuntang Hulu Sigar, Abner Darmawan; Susetyo, Yefta Audy; Mustikasari, Rosita; Simanjuntak, Bistok Hasiholan; Setiawan, Andree Wijaya; Banjarnahor, Dina
Jurnal Pertanian Terpadu Vol 13 No 2 (2025): Jurnal Pertanian Terpadu Jilid XIII Nomor 2 Desember 2025
Publisher : Sekolah Tinggi Pertanian Kutai Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36084/jpt..v13i2.673

Abstract

Simpanan karbon organik tanah berperan penting dalam mitigasi perubahan iklim dan menjaga keberlanjutan sistem pertanian. Penelitian ini menganalisis simpanan karbon tanah pada tiga jenis lahan pertanian di Sub-DAS Tuntang Hulu, yaitu sawah irigasi, sawah tadah hujan, dan tegalan. Kandungan karbon organik diukur menggunakan metode gravimetri, dan simpanan karbon total dihitung berdasarkan bobot isi tanah dan kedalaman 0–30 cm. Survei praktik budidaya dan wawancara petani dilakukan untuk mengidentifikasi faktor yang berpengaruh terhadap akumulasi karbon tanah. Hasil menunjukkan bahwa lahan tegalan memiliki simpanan karbon rata-rata tertinggi. Namun, hubungan antara praktik budidaya seperti pengembalian sisa tanaman, pengolahan tanah, pemupukan, dan kepemilikan ternak dengan kandungan karbon tidak menunjukkan signifikansi statistik. Praktik budidaya yang mendukung ketahanan cadangan karbon tanah secara berurutan adalah tegalan, sawah tadah hujan dan sawah irigasi.
Evaluasi Ketahanan Beberapa Varietas Padi terhadap Penyakit Busuk Pelepah Akibat Infeksi Fusarium longipes Verrel Santosa; Ruth Meike Jayanti; Maria Marina Herawati; Andree Wijaya Setiawan
Agriprima : Journal of Applied Agricultural Sciences Vol 10 No 1 (2026): MARCH
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v10i1.805

Abstract

Penyakit busuk pelepah merupakan salah satu penyakit penting pada tanaman padi yang dilaporkan semakin meluas dan berdampak signifikan terhadap penurunan hasil. Selain Sarocladium oryzae, beberapa spesies Fusarium, termasuk Fusarium longipes, diketahui berasosiasi dengan penyakit ini dan berpotensi memperparah kerusakan tanaman. Penelitian ini bertujuan untuk mengevaluasi tingkat ketahanan beberapa varietas padi terhadap penyakit busuk pelepah akibat infeksi F. longipes berdasarkan keparahan penyakit dan parameter epidemiologi. Penelitian dilaksanakan di rumah kaca menggunakan sepuluh varietas padi, yaitu Ciherang, Sunggal, Inpari 32, Inpari 42, Inpari 43, Memberamo, Situ Bagendit, IR64, Padjadjaran, dan SRP08, yang disusun dalam rancangan acak kelompok dengan tiga ulangan. Inokulasi dilakukan pada umur 60 hari setelah tanam menggunakan bulir beras yang telah dikoloni F. longipes. Pengamatan keparahan penyakit dilakukan pada 3, 6, 9, 12, dan 15 hari setelah inokulasi. Hasil penelitian menunjukkan bahwa keparahan penyakit meningkat seiring waktu pengamatan dan berbeda antarvarietas. Pada akhir pengamatan, seluruh varietas menunjukkan keparahan penyakit yang tinggi dan diklasifikasikan sebagai sangat rentan berdasarkan kriteria IRRI. Nilai laju infeksi dan Area Under Disease Progress Curve (AUDPC) menunjukkan adanya perbedaan dinamika perkembangan penyakit antarvarietas, meskipun tidak ditemukan varietas dengan tingkat ketahanan efektif terhadap infeksi F. longipes. Hasil penelitian ini menegaskan pentingnya evaluasi ketahanan varietas sebagai dasar pengelolaan penyakit busuk pelepah dan pengembangan varietas padi yang lebih adaptif.
Uji Efektivitas Ekstrak Daun Cengkih dan Jintan untuk Menurunkan Intensitas Serangan Penyakit Antraknosa pada Tanaman Cabai Besar Jimmy Dwi Cahya; Andree Wijaya Setiawan
Jurnal Ilmiah Membangun Desa dan Pertanian Vol. 9 No. 2 (2024)
Publisher : Department of Agribusiness, Halu Oleo University Jointly with Perhimpunan Ekonomi Pertanian Indonesia - Indonesian Society of Agricultural Economics (PERHEPI/ISAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37149/jimdp.v9i2.1078

Abstract

Anthracnose disease is one of the diseases in chili plants that can reduce yields by up to 80%. This study aims to determine the effectiveness and optimal concentration of natural fungicides from cloves and cumin in reducing the incidence and intensity of anthracnose disease (Colletotrichum sp.) attack directly on large chili plants in the field. The research was conducted in Sumberejo Village, Ngablak District, Magelang Regency. The study used a completely randomized design (CRD) with eight treatments and four replications. The extract treatments used clove leaves and cumin leaves with concentrations of 1%, 2%, and 3%, and no treatment and synthetic fungicides. The variables observed in this study were disease incidence, intensity of attack, and total fruit weight at harvest. The use of pesticides in the study showed significant differences from that of the control. The best natural fungicides for disease incidence in this study were CK3 (1,79%) and JK3 (2,94%), while the best natural fungicide treatments for attack intensity in this study were CK3 (11,25%) and JK3 (16,25%). Applying clove and cumin leaf fungicides to plants does not reduce crop yields.
Uji Efektivitas Tanaman Refugia dalam Menurunkan Intensitas Serangan Lalat Buah pada Cabai Rawit Albertus Edy Kurniawan; Andree Wijaya Setiawan
Jurnal Ilmiah Membangun Desa dan Pertanian Vol. 9 No. 2 (2024)
Publisher : Department of Agribusiness, Halu Oleo University Jointly with Perhimpunan Ekonomi Pertanian Indonesia - Indonesian Society of Agricultural Economics (PERHEPI/ISAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37149/jimdp.v9i2.1110

Abstract

Fruit flies (Bactrocera spp.) are pests that attack cayenne peppers, with attacks reaching 100%. Generally, farmers use synthetic insecticides, but in the long term this can disrupt the balance of the ecosystem and trigger pest outbreaks and resistance—integrated pest control by planting refugia as a micro-habitat for natural enemies. Marigolds and zinnias were chosen as refugia plants. The aim of the research was to determine the types of fruit flies that attack chili plants, the symptoms of fruit fly attacks on chili fruit, the effectiveness of marigold and zinnia plants in reducing the intensity of chili plant attacks, and external factors that influence the efficacy of refugia marigold and zinnia plants. The research was conducted in July – October 2023 in Jlarem Village, Gladaksari District, Boyolali Regency, using a Randomized Block Design (RAK) with four treatments, namely control, marigold, zinnia, and a combination of both, using six replications so that there were 24 experimental units with ten plants in each experimental unit. This research used a hygrometer thermometer (HTC2), camera, net, and binocular microscope. Observation variables include the type of fruit fly, attack symptoms, attack intensity, and external factors. The types of fruit flies found in the study area were Bactrocera dorsalis, Bactrocera umbrosa, and Bactrocera carambolae. Symptoms that arise due to fruit fly attacks on chili fruit are black spots on the skin of the fruit, which become more expansive over time, causing the fruit to rot and fall prematurely. Refugia planting effectively reduced the intensity of fruit fly attacks, with the lowest attack intensity in the combination treatment of marigold and zinnia at 22.16%. Several external factors influence the effectiveness of refugia in controlling fruit flies, namely environmental temperature and humidity and the types of plants growing around the cultivated land.