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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Veteriner Medical Journal of Indonesia Amerta Nutrition The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy
Sri Estuningsih
Departemen Klinik Reproduksi Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary Other

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Journal : Jurnal Ilmu Pertanian Indonesia

 1 
Deteksi Dini Penyakit Tumor Sel Darah Myelosit Leukosis melalui Pemeriksaan Ulas Darah Dewi Ratih Agungpriyono; Hernomoadi Huminto; Sri Estuningsih; Aryani Sismin Satyaningtijas
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.708 KB)

Abstract

Myelocytes blood cell tumor in chicken is a disease caused by retrovirus, avian leukosis virus subgroup J (ALV-J). The virus has the same group as human retrovirus (HIV) which caused AIDS, but the avian type possesses oncogenic properties, that could induce cell transformation and tumor formation. ALV-J stimulates the bone marrow's myelocytes and transforms them into tumor cell myelocytoma. The tumor cells then metastasis through the circulatory system causing myeloid leukosis and tumor cells accumulation in various internal organs or myelocytomatosis. This study was done on the base of the leukosis behavior of the tumor. The finding of the metastasis tumor cell in the blood smear examination is thought could be use as the diagnostic clue of the disease. Blood smear from sick chickens are collected and stained with some chemical staining substance such as may grunwald-giemsa, hematoxyllin eosin, periodic acid Schiff, congo red, toluidine blue, and sudan black B. Cytochemistry character of the blood cells was observed using light microscope. The result showed that myelocytes granules were best observed using hematoxyllin eosin, periodic acid Schiff, congo red and toluidine blue while may grunwald-giemsa, and sudan black B could not differentiate the granules. By this method, the field veterinarian will able to screen the suspected chicken flock for myeloid leukosis earlier than the occurrence of tumor formation.
Seleksi, Karakterisasi Morfologi, dan Identifikasi Aktinobakteri Penghasil Mananase Asal Hutan Tanah Jambi untuk Produksi Mananoligosakarida Rahayu Wulan; Rika Indri Astuti; Yaya Rukayadi; Sri Estuningsih; Anja Meryandini
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.279

Abstract

As the world's largest producer of crude palm oil, Indonesia also produces high palm kernel cake (PKC) by-products. PKC has a high mannan content, so it can be used to produce prebiotic mannan-oligosaccharides (MOS). Enzymatic MOS production can be carried out using actinobacterial mannanase's microbiological approach. The HJ45B-1 isolate was the best isolate, with a peak enzyme production of 0,338 U/mL on the 10th day of incubation. The mannanase enzyme was stable in storage at 27°C. MOS production using 1% PKC substrate produced MOS with the best degree of polymerization (2-4) with incubation for 1-3 hours. Morphological characteristics and molecular identification based on the 16S rRNA gene indicated that the HJ45B-1 isolate was Streptomyces spp. Keywords: actinobacteria, mannanase, mannan-oligosaccharides, palm kernel cake, Streptomyces
Co-Authors A.S. Satyaningtijas Ahmad Sulaeman Anja Meryandini Arlita Sariningrum Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto Budi Setiawan Chudahman Manan Chudahman Manan Daldiyono Daldiyono Daldiyono Daldiyono Deni Noviana Dewi R. Agungpriyono Dewi Ratih Agungpriyono Dian Masyitha Eddy Widodo Edward, Syafri Faisal Amri Satrio Hendriana, Okta Hernomoadi Huminto Maggy T. Suhartono Mokhamad Fakhrul Ulum, Mokhamad Ni Wayan Kurniani Karja Nugrahaeni, Mutiara Nurliana . Rahayu Wulan Rahminiwati, Min Ratih Dewanti -Hariyadi Rika Indri Astuti Robert Utji Satrija, Esdinawan Carakantara Siti Nurjanah sri murtini . Sugito Sugito Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Yaya Rukayadi Yenny Rakhmawati Zuraidah Nasution