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MP-12 Effect of AKBISprob Supplementation on Antibacterial-Producing Lactic Acid Bacteria (LAB) Isolated from Laying Hens Intestine . Nurliana; Sema Coumandary; Fashihah Rahmah Noya Farimansysh; . Sugito; Dian Masyitha; . Darmawi; . Erina; . Gholib; Erdiansya Rahmi; . Ismail
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Generally, Bacteria in the intestine consist of beneficial bacteria and bacteria that have the potential to disrupt animal health such as pathogenic bacteria. The presence of pathogenic bacteria is one of the factors that cause gastrointestinal dysfunction, but can be suppressed by maintaining the presence of beneficial bacteria for the digestive tract, especially the type of Lactic Acid Bacteria (LAB) (Astuti, 2016). LAB has antagonistic activity because it is able to inhibit pathogenic bacteria so it can compete to maintain normal flora balance in the digestive tract.  The main inhibitory activity of LAB is caused by the accumulation of primary metabolites such as lactic acid, acetic acid, ethanol, and carbon dioxide. In addition, LAB is also capable of producing antibacterial compounds such as bacteriocins (Furtado et al., 2014). Naturally LAB has existed in the digestive tract of chickens, but it is easy to experience changes in the amount due to the influence of given the feed (Widodo et al., 2015). Feed and the environment can affect the composition of microbes in the digestive tract in chickens(Ghadban, 2002; Apajalahti et al., 2004). Provision of fermented feed can improve the composition of intestinal microflora and increase the number of LAB. Fermented feed is generally easily biodegradable and has a higher nutritional value than the original ingredient and it can reduce the pH of the digestive tract.AKBISprob is an alternative product to increase poultry production in the form of supplement made from a mixture of soybean wate, and palm kernel meal which is fermented with Aspergillus niger.  The fermentation process using Aspergillus niger can reduce the crude fiber contained in AKBIS, because the mold can produce amylase, pectinase, amyloglucosidase and cellulase enzymes which can degrade cellulose so that it is easier to digest. In addition, Aspergillus niger also produces metabolites in the form of citric acid which is a component that can reduce intestinal pH so that it is suitable for LAB growth. Based on research conducted by Nurliana et al. (2016; 2017), supplementation of 4% AKBISprob can maintain the chickens production and health as well increase the number of LAB and reduce the number of pathogenic bacteria such as Escherichia coli and Salmonella sp. in chickens intestine. Thus creating a balance of microflora in the digestive tract of laying hens. Based on the above reasons, it is necessary to conduct research on antibacterial detection of BAL in laying hens that have been given AKBISprob.
Anticandidal in vitro test of crude ethanol extract of pliek u on the growth of Candida albicans . Nurliana; . Rinidar; Dian Masyitha
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This research was conducted to know the activity of crude ethanol extract of pliek u (E) through in vitro assays. Pliek u obtained from households industry in the Reudep village Aceh Besar, Aceh province. Yeasts used in clinical trials is Candida albicans isolates obtained from the Mycology Laboratory of the Faculty of Veterinary Medicine, Bogor Agricultural University. Anticandidal activity was determined on a paper disc, whereas in the broth dilution method is used to set a killing time activity of the E. The concentration of E that is used to determine the killing time activity is the value of LC50. Crude ethanol extract of pliek u (E) showed anticandidal activity with inhibitory zone diameters of 10.67 ± 0.47 mm. Ethanol extract of pliek u at a concentration of 3.36 mg / ml can reduce the number of C. albicans within two hours at 2.57 log CFU / ml compared to controls at 7.42 log CFU / ml. Based on this study can be concluded that ethanol extract of pliek u (E) can be considered an alternative drug for treating candidiasis, so it needs further research to veterinary clinics in vivo applications
Effect of addition pliek U in feed on histomorphometric of small intestine villi of broiler Azmi Zul; . Nurliana; Ummu Balqis; Dian Masyitha
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The aims of this research was to find the effect of pliek u on the morphometric of small intestine villi of broiler. This research was conducted at teaching farm and Pathology Laboratory, Veterinary Medicine Faculty, Syiah Kuala University, on June to November 2011. Pliek u was collected from home industry in Jangka village, Bireuen. Pliek u added in feed with concentration of 0.5%, 1%, dan 2%, that was given every day for 28 days. Complete Randomized Design (CRD) was used in this research with four treatments K0 (control), K1 (0.5% pliek u), K2(1% plieku), and K3(2% pliek u), each treatment group contain three broiler. The results showed that the concentration of addition pliek u in feed had no effect to histomorphometric of small intestine villi of broiler
Stabilitas Mikrob Usus, Histologi Hati dan Ginjal Mencit Setelah Pemberian Ekstrak Pliek u Bumbu Masak Tradisional Aceh (STABILITY OF GUT MICROBIAL AND HISTOLOGY OF LIVER AND KIDNEY OF MICE AFTER ADMINISTRATION OF PLIEK-U EXTRACT Nurliana .; Sri Estuningsih; Sugito .; Dian Masyitha
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Pliek u is one of the traditional spices of Aceh prepared by fermentation of coconut meat which is usedas chicken feed additive too. The stability of gut micro flora and histopathological changes of liver andkidney of mice were detected after three days oral administration of acute single dose of ethanol extract ofpliek u. Nine animals were used and grouped into three; in which group I and II (treatment groups) wereadministered 370 and 733 mg/kg body weight of pliek u, respectively, and group III referred as the control.At the fourth day of experiment, all animals were sacrificed, and their livers, kidneys and intestinal gutwere excised. The gut microbial was measured by Total Plate Count (TPC). Livers and kidneys wereprocessed for paraffin procedure and hematoxyllin-eosin staining. The results showed that the ethanolextract of pliek u has no significant effect (P>0.05) on the TPC of the gut microbial and the structure of theliver and kidney of mice. It can be concluded that administration of the two doses (370 and 730 mg/kg bw)of pliek u did not lowering the numbers of gut microbial and were not toxic to the livers and kidneys of themice.
STUDI HISTOLOGIS LAMBUNG SAPI ACEH. (Histological Study of Gastric in Aceh Cattle). roza Agravion; Dian Masyitha; Zainuddin Zainuddin; M Jalaluddin; Nazaruddin Nazaruddin; Arman Sayuti
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (929.412 KB) | DOI: 10.21157/jim vet..v2i3.7819

Abstract

Telah dilakukan penelitian tentang struktur histologi lambung (rumen, retikulum, omasum, dan abomasum) sapi aceh. Penelitian ini bertujuan mengetahui struktur histologi lambung sapi aceh. Sampel penelitian diambil dari 3 ekor sapi aceh berjenis kelamin jantan, telah dewasa kelamin yang dipotong di Rumah Potong Hewan di Aceh Besar. Terhadap sampel penelitian dilakukan proses mikroteknik untuk selanjutnya dilakukan pewarnaan Hematoksilin-eosin (HE). Pengamatan terhadap struktur histologi menggunakan mikroskop cahaya binokuler. Berdasarkan hasil penelitian, dapat disimpulkan bahwa struktur histologi lambung sapi aceh tidak berbeda dengan struktur histologi lambung ruminansia lainnya (sapi, domba, dan kambing), yaitu terdiri dari empat lapisan, tunika mukosa, tunika submukosa, tunika muskularis, dan tunika serosa. Lamina propria rumen menyatu dengan submukosa membentuk propria-submukosa dan terdapatnya kapiler fenestrated dibawah membran basal. Ciri khas dari retikulum sapi aceh terdapat pada lamina muskularis mukosa yang membentuk lipatan memanjang. Tunika mukosa abomasum terdapatnya kelenjar yaitu kelenjar kardia, fundus, dan pilorus.(A Study to detect the microscopic structure of gastric (rumen, reticulum, omasum, and abomasum) in aceh cattle. The purpose of this research was to know the histological structure of the gastric in aceh cattle. The samples were collected from 3 of male aceh cattle in Aceh Besar abattoir. The tissue samples were processed by microtechnique and Hematoksilin-eosin (HE). Microscopic analysis was performed using binocular light microscope. The study showed that the wall of gastric aceh cattle not different with another ruminasia (cow, sheep, and goat), they are made up of four layers, that was tunica mucosa, submucosa, muscularis, and serosa. The mucosa segment there are lamina epithelia, lamina propria, and lamina mucosa muscularis. At rumen, lamina propria merges to submucosa created submucosa-propria and founded fenestrated capiler under epithelium basal membran. The characterstic of reticulum based on mucosa muscularis which is create fold lengthwise. Mucosa of abomasum founded some glands, there are cardiac, fundic, and pyloric). 
GAMBARAN HISTOLOGI SALURAN PENCERNAAN IKAN GABUS (Channa striata) (Histological of Alimentary Canal in Snakehead Fish (Channa striata)) Muhammad Nafis; Zainuddin zainuddin; Dian Masyitha
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 2 (2017): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (770.909 KB) | DOI: 10.21157/jim vet..v1i2.3141

Abstract

ABSTRAK            Penelitian ini bertujuan untuk mempelajari struktur histologi saluran pencernaan ikan gabus (Channa striata). Saluran pencernaan yang diambil adalah esofagus, lambung, dan usus depan berasal dari dua ekor ikan gabus. Sampel kemudian dibuat menjadi preparat histologi dengan pewarnaan hematoksilin-eosin (HE) dan diamati menggunakan metode histologi eksplorasi. Hasil penelitian menunjukkan bahwa saluran pencernaan tersusun atas empat lapisan, yaitu tunika mukosa, submukosa, muskularis, dan serosa. Tunika mukosa terdiri dari lamina epitelia, lamina propria, dan lamina muskularis mukosa. Tunika submukosa terdiri dari jaringan ikat dengan pembuluh darah, limfe dan saraf. Tunika muskularis tersusun atas otot melingkar dan otot memanjang. Tunika serosa terdiri dari lapisan tipis jaringan ikat yang dilapisi oleh epitel pipih selapis (mesotelium) dengan pembuluh darah dan jaringan lemak. Mukosa esofagus membentuk lipatan seperti vili-vili dengan epitel pipih berlapis dan banyak sel-sel mukosit, tunika muskularis tersusun atas otot lurik. Mukosa lambung terdiri dari epitel silindris selapis, terdapat kelenjar lambung pada lamina propria, tunika muskularis terdiri dari otot melingkar dan memanjang. Mukosa usus yang membentuk vili tersusun atas epitel silindris selapis dengan mikrovili dan sel goblet, tidak ditemukannya kelenjar Brunner maupun Liberkhun.ABSTRACTThis research aims to study the histological structure of the alimentary canal in snakehead fish (Channa striata). The alimentary tract taken were oesophagus, gastric, and anterior intestines which were derived from two snakehead fish. Histological samples stained with haematoxylin-eosin (HE) then observed using explorative histological method. The results showed that the alimentary canal is composed of four layers, that is tunica mucosa, submucosa, muscularis, and serosa. The tunica mucosa consists of lamina epithelia, propria, and muscularis mucosa. The tunica submucosa consists of connective tissue with blood vessels, lymph vessels, and nerves. The tunica muscularis were composed of circular and longitudinal muscles. The tunica serosa consist of a thin layer of connective tissue that is coated by simplex squamous epithelium (mesothelium) with blood vessels and adipose tissue. Oesophagus mucosa then folds forming villi which is coated by stratified squamous epithelium and many mucosit cells, skeletal muscle in tunica muscularis. Gastric mucosa consists of simplex columnar epithelium, glands of gastric on the lamina propria, tunica muscularis consists of circular and longitudinal muscle. Intestinal mucosa which forming villi is composed of simplex columnar epithelium with microvilli and goblet cells, Brunner and Liberkhun glands were not found.
STUDI HISTOLOGIS USUS HALUS SAPI ACEH (Histological Study Small Intestine of Aceh Cattle) Adi Firmansyah; Dian Masyitha; Zainuddin Zainuddin; Fitriani Fitriani; Ummu Balqis; Fadli A Gani; Azhar Azhar
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 4 (2019): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v3i4.7235

Abstract

ABSTRAKTelah dilakukan penelitian tentang struktur histologis usus halus sapi aceh yang terdiri atas duodenum, jejunum dan ileum. Penelitian ini bertujuan mengetahui struktur histologi usus halus sapi aceh. Sampel penelitian diambil dari 3 ekor sapi aceh yang telah dewasa kelamin dan berjenis kelamin jantan yang dipotong di Rumah Potong Hewan Lambaro Aceh Besar. Terhadap sampel penelitian dilakukan proses mikroteknik untuk selanjutnya dilakukan pewarnaan Hematoksilin-eosin (HE). Pengamatan terhadap struktur histologi menggunakan mikroskop cahaya binokuler pembesaran 40x, 100x, dan 400x. Hasil penelitian menunjukkan, struktur histologi duodenum, jejunum dan ileum sapi aceh tersusun atas empat lapisan, yaitu tunika mukosa, tunika submukosa, tunika muskularis, dan tunika serosa. Tunika mukosa duodenum, jejunum dan ileum tersusun oleh epitel silindris selapis dan terdapat sel goblet. Ketebalan tunika mukosa duodenum yaitu: (396,68±6,5 µm), Jejunum (339,46±13 µm), dan Ileum (451,92±6,5 µm). Tunika submukosa terdiri dari jaringan ikat longgar, buluh darah dan saraf. Tunika submukosa duodenum terdapat kelenjar brunner dan pada ileum terdapat nodulus limfoideus dengan ketebalan duodenum yaitu: (344,4±10 µm), Jejunum (227,98±7,8 µm), dan ileum (330,35±5,7 µm). Tunika muskularis tersusun oleh otot polos sirkular dan longitudinal dengan ketebalan masing-masing yaitu, duodenum (973,47±5,5 µm), Jejunum (475,5±9,8 µm)  dan ileum (670,51±13 µm). Tunika serosa merupakan lapisan paling luar dari usus halus dengan ketebalan berturut-turut yaitu, (335,34 ±7,4 µm) duodenum, (231,33±6,9 µm) jejunum dan (354,67±11 µm) ileum.Kata kunci: Usus halus, duodenum, jejunum, ileum, sapi aceh ABSTRACTA Study to detect the microscopic structure of small intestine (duodenum, jejunum and ileum) of aceh cattle. The aims of this research was to know the histological structure of the small intestine in aceh cattle. The samples were collected from 3 of male aceh cattle in Lambaro Aceh Besar abattoir. The tissue samples were processed by microtechnique and Hematoksilin-eosin (HE). Microscopic analysis was performed using binocular light microscope 40x, 100 x, and 400x. The study showed that the wall of the duodenum, jejunum, and ileum are made up of four layers, that was tunica mucosa, submucosa, muscularis, and serosa. Tunica mucosa duodenum, jejunum and ileum consisted of ephitelium simple columnar cells and goblet celss. The thick of tunica mucosa duodenum are (396,68±6,5 µm), jejunum (339,46±13 µm), and ileum (451,92±6,5 µm).  The submucosa contain connective tissue, arteriole, venole and nervous. The submucosa duodenum conside of glands brunners and ileum of nodulus limfoideus and thick of tunica submucosa duodenum are (344,4±10 µm),  jejunum (227,98±7,8 µm), and ileum (330,35±5,7 µm). Tunica muscularis consists of two layers of smooth muscle inner circular and longitudinal. The thick of tunica mucularis are (973,47±5,5 µm) duodenum, (475,5±9,8 µm) jejunum and (670,51±13 µm) ileum. The tunika serosa forms the outermost layer with thick (335,34 ±7,4 µm) duodenum, (231,33±6,9 µm), jejunum and (354,67±11 µm) ileum.Keyword: Small intestine, duodenum, jejunum, ileum, sapi aceh
DENSITAS RADIOGRAFI TULANG FEMUR ANJING LOKAL (Canis lupus familiaris) YANG DIOVARIOHISTEREKTOMI (Radiography Density of Femur Bone On Lokal Dogs (Canis lupus familiaris) That In Ovariohisterektomi) Dinda Jasmal Sikumbang; Budianto Panjaitan; Syafruddin Syafruddin; Erwin Erwin; Dian Masyitha; Hamdan Hamdan
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.32 KB) | DOI: 10.21157/jim vet..v2i3.7815

Abstract

Penelitian ini bertujuan untuk mendapatkan gambaran densitas radiografi tulang femur pada anjing lokal. Hewan yang digunakan dalam penelitian ini adalah 3 ekor anjing betina lokal dengan kisaran umur 5-7 bulan. Pengambilan gambar radiografi dan pengukuran densitas radiografi tulang femur dengan menggunakan Software Image J pada minggu ke 0 (sebelum diovariohisterektomi), minggu ke 1, 2, 3, dan 4 (setelah diovariohisterektomi). Hasil penelitian menunjukkan bahwa rata-rata densitas radiografi tulang femur sebelum diovariohisterektomi adalah 151,8401±5,8767. Hasil ini menunjukkan berbeda nyata  pada  minggu 1, 2, dan 4 setelah diovariohisterektomi dengan hasil densitas radiografi tulang 168,7952±7,88446, 181,0557±7,81876, dan 169,1759±4,73123. Pada minggu 3 setelah diovariohisterektomi menunjukkan hasil tidak berbeda nyata dengan densitas radiografi tulang sebelum diovariohisterektomi yaitu 152,9360±7,38131. Berdasarkan hasil penelitian dapat disimpulkan bahwa densitas tulang dari hasil pengambilan gambar radiografi tulang femur anjing lokal (Canis lupus familiaris) didapatkan terjadi peningkatan densitas radiografi pada minggu ke 1, 2, 4 dan menurun pada minggu 3. Penurunan dan peningkatan densitas radiografi pada tulang femur bersifat fluktuasi. (This study aimed  to get radiodensity of femoral bone in local dog. Animals used in this study were 3 local female dogs with the age range of 5-7 months. Radiographic imaging and radiographic density measurements of femur bone using Software Image J before and after ovariohysterectomy at weeks 1, 2, 3, and 4. The results show that the average radiodensity of femur bone before ovariohysterectomy was 151,8401± 5,8767. This result had significantly different with 1, 2, and 4 weeks after ovariohysterectomy. In week 3 after ovariohysterectomy, the results were not significantly different from the radiograph density of the femur bone before in ovariohysterectomy that was 152.9360 ± 7.38131. Based on the results of the study it can be concluded that bone density resulting from radiography imaging of local dog's femur (Canis lupus familiaris) using Image Software Image J obtained  radiographic density increased at week 1, 2, 4 and decreased at week 3. The decrease and increase of radiographic density of the femur bone is a fluctuation).
DENSITAS RADIOGRAFI TULANG HUMERUS ANJING LOKAL (Canis lupus familiaris) YANG DI OVARIOHISTEREKTOMI. (Radiography Density Of Humerus Bone On Local Ovariohisterektomi Dogs (Canis lupus familiaris). meutya silviana morow; budianto panjaitan; syafruddin syafruddin; dian masyitha; erwin erwin; cut nila tasmi
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.168 KB) | DOI: 10.21157/jim vet..v2i3.7907

Abstract

ABSTRAKPenelitian ini dilakukan bertujuan untuk mendapatkan gambaran densitas radiografi tulang humerus pada anjing lokal (Canis lupus familiaris) sebelum dan sesudah dilakukan ovariohisterektomi. Penelitian ini menggunakan tiga ekor anjing betina lokal dengan kisaran umur 5-7 bulan. Teknik perlakuan diambil  gambar radiografi sebelum dilakukan ovariohisterektomi selanjutnya semua hewan dilakukan ovariohisterektomi melalui linea alba, setelah itu hewan di ovariohisterektomi. Pada minggu 1,2,3, dan 4 setelah di ovariohisterektomi dilakukan pengambilan gambar radiografi dan pengukuran densitas tulang. Hasil penelitian menunjukkan bahwa rata-rata densitas tulang pada minggu 0 sebelum ovariohisterektomi adalah 157.3661667 ± 23.3043699, hasil ini tidak berbeda nyata dengan hasil rata-rata densitas pada minggu 1,3, dan 4 yaitu 156.8621982 ± 12.07528008, 153.8854886 ± 15.93987235, dan 156.1769175 ± 19.84084989, perbedaan terjadi pada minggu ke 2 dengan hasil 144.6901974 ±10.76999831. Berdasarkan hasil penelitian dapat disimpulkan  bahwa penurunan densitas radiografi tulang humerus hanya bersifat sementara pada minggu ke 2 terjadi penurunan kemudian meningkat kembali pada minggu ke 3 dan minggu ke 4.ABSTRACTThe aim of this study was to obtain a picture of radiographic density of humerus bone in local dog (Canis lupus familiaris) before and after ovarianohysterectomy. This study used three local female dogs with the age range of 5-7 months. The technique of treatment is taken radiographic images before the next ovarianohysterectomy is done all animals are done ovariohisterektomi through linea alba, after which the animal in ovariohisterektomi. At weeks 1,2,3 and 4 after ovarianohysterectomy, radiographic imaging and bone density measurements were performed. The results showed that the average bone density at week 0 before ovariohisterectomy was 157.3661667 ± 23.3043699, this result was not significantly different with the average density results at weeks 1.3 and 4, ie 156.8621982 ± 12.07528008, 153.8854886 ± 15.93987235, and 156.1769175 ± 19.84084989, the difference occurs at week 2 with the result 144.6901974 ± 10.76999831. Based on the results of this study it can be concluded that degradation of humerus bone radiography density is only temporary at 2 weeks decrease then increase again at week 3 and week 4.
STRUKTUR HISTOLOGI KULIT BELUT SAWAH (Monopterus albus) (HISTOLOGY OF SKIN OF RICE FIELD EELS (Monopterus albus) ) Nanda Afrizan; zainuddin zainuddin; cut dahlia iskandar; dian masyitha; winaruddin winaruddin; ummu balqis
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.739 KB) | DOI: 10.21157/jim vet..v2i2.7606

Abstract

Penelitian ini bertujuan mengetahui struktur histologi kulit ikan belut sawah. Sampel yang digunakan adalah kulit bagian dorsal dan abdomen dari dua ekor belut sawah, diamati dengan menggunakan metode histologi eksplorasi. Pengamatan dilakukan setelah pembuatan preparat histologi yang diwarnai dengan Hematoksilin-eosin (HE) dan Masson trichrome. Hasil penelitian menunjukkan struktur histologi kulit ikan belut sawah terdiri atas tiga lapisan. Lapisan epidermis yang tersusun atas sel-sel epitel pipih berlapis, sel mukus/sel goblet, dan stratum germinativum. Lapisan dermis terdiri dari sel pigmen, stratum laxum (spongiosum) dengan serabut kolagen longgar dan stratum compactum dengan serabut kolagen padat. Lapisan hipodermis terdiri dari sel lemak, serabut kolagen, dan pembuluh darah. Secara umum struktur histologi kulit belut sawah sama dengan ikan air tawar pada umumnya, terdapat perbedaan ketebalan lapisan dan bentuk sel mukus antara kulit dorsal dan abdomen kulit belut sawah. (This research aims to determine the histological structure of rice eels. The sample used is the skin of the dorsal and abdominal and then observed using histologcal  exploratory method. Observations were made after the preparation of histologic preparations stained with hematoxylin-eosin (HE) and Masson trichrome. The results showed that the histology structure of the rice eels consists of three layers. Epidermal layers composed of squamous cells, mucus / goblet cells, and stratum germinativum. The dermis layer consists of pigment cells, stratum laxum (spongiosum) with loose collagen fibers and stratum compactum with solid collagen fiber. The hypodermic layer consists of fat cells, collagen fibers, and blood vessel. In general the structure of rice field eel skin histology identical with freshwater fish in General, there is a difference in thickness of the layers and the shape of the dorsal skin mucous cells between the abdominal skin an eel and rice fields.)