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All Journal Prosiding Seminar Biologi JOURNAL ONLINE OF PHYSICS JURNAL AGRILAND BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Faktor Exacta Dinamisia: Jurnal Pengabdian Kepada Masyarakat Bioedukasi: Jurnal Pendidikan Biologi Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Jurnal Biosense Sciening : Science Learning Journal Bioscientist : Jurnal Ilmiah Biologi EduBiologia: Biological Science and Education Journal Berkala Penelitian Hayati Bioscientiae, Jurnal ilmu ilmu Biologi Jurnal Pengabdian Masyarakat Biologi dan Sains Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)

Fitri Damayanti

Universitas Indraprasta PGRI

Author-ID : 2638565

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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VARIASI SOMAKLONAL TANAMAN KANTONG SEMAR (NEPENTHES MIRABILIS DAN N. GRACILIS) SECARA IN VITRO DENGAN MUTAGEN KIMIA KOLKISIN FITRI DAMAYANTI; IKA ROOSTIKA
Faktor Exacta Vol 8, No 3 (2015)
Publisher : LPPM

Nepenthes are potential to be commercialized because of the shape and color of their unique pitcher. Generally, people are interested in new and rare types which have unique color, shape, and size of pitcher. However, the increase of its demand has led to the decrease of natural populations due to overexploitation in the natural habitats in Indonesian forests without sufficient cultivation or replacement efforts. The genetic erosion has resulted in low level variation of this species. In vitro plant breeding can be carried out through somaclonal variation. The objective of this study was to obtain somaclonal variation method by using colchicin as a chemical mutagen. In vitro shoot of N. mirabilis and N. gracilis were used in this research as explant that planted in ¼ MS + colchicin at the level of 0, 0.05, 0.075, and 0,1%. The application of colchicin could produced cultures with high level of growth than control. The best result was obtained from colchicin at a level of 0.05% as a alternative in vitro to improve genetic variation. Key words: N. mirabilis, N. gracilis, somackonal variation, colchicin

KOLEKSI PLASMA NUTFAH PISANG SECARA EX VITRO DAN IN VITRO SERTA KAJIAN SITOLOGI DAN ANALISA KERAGAMAN ANTAR KARAKTER BERDASARKAN PENANDA FENOTIPE Fitri Damayanti; Ika Roostika
Faktor Exacta Vol 3, No 2 (2010): Faktor Exacta
Publisher : LPPM

Indonesia is the one of banana biodiversity center in the world. Genetic diversity of this plant is large. Thus, it is important to conserve these genetic resources to support banana breeding program. Collection of banana germplasm is more important due to over exploitation in the natural habitats in Indonesian forests without sufficient culture or replacement efforts. Studies on cytology and morphological characters of plants are required to avoid duplications of germplasm collections. This research would be explored 30 accession of Indonesian banana germplasms from East Borneo, Banjarmasin (South Borneo), field germplasms from Cibinong, Yogyakarta, Rembang, Purworejo, and Subang. There were 20 accession of germplasms collections that would succeed to collect in vitro. Chromosome analysis revealed different ploidies: diploid (2n=2x=22) and triploid (2n=3x=33). Related similarity in dendogram from ten accessions of banana (Rotan, Telunjuk, Emas, Hutan, Lampung, Api Merah, Api Hijau, Morosebo, Tanduk, and Barangan) based of vegetative and generative characters divided into four groups at 88.75% similarity; group I were accessions of Emas, Telunjuk, Rotan, and Hutan;accessions of Lampung and Morosebo were group II; group III was accession of Tanduk; and the last group were accessions of Api Merah, Api Hijau and Barangan. Result from in vitro experiment, induction of banana bud was depending of fenolic acid that results from specific variety, growth hormone concentration, and genome. Banana with A genome (diploid AA or triploid AAA) had the best growth because the result of fenolic acid was lower. Culture media with 5 ppm BAP was elevating in vitro bud of banana germplasm that have more number of A genome. The banana whose have more number of B genome was need higher concentration of BAP. Ambon Kuning whose have AAA genome had the higher multiplication of bud rather than Api Hijau and Api Merah whose have AAB genome or Kepok Kuning with ABB genome. Key words: genome, chromosome, Musa spp., ploidy, stomata

Co-Authors . SUHARSONO Acep Musliman Ardianti Saputri Aris Tjahjoleksono Aryanto, Saring Avini Nurazhimah Arfa Ayelir Sukma Yolla Dheani Amalia Firdaus Dini Siti Nurahmah Efri Gresinta Emilda Emilda Erik Perdana Putra Fitri Damayanti Giry Marhento Ika Mariska Ika Mariska IKA ROOSTIKA Ika Roostika Ika Roostika Linda, Linda Mashudi Alamsyah MUHAMMAD MANSUR Muhammad Mansur Pina Oktapiani Rifqi Pratama Rosa Dewi Pratiwi Sri Murni Soenarno Suharsono Suharsono Supriyatin, Titin Sutoro Sutoro Titin Supriyatin Tulus Widjajanto Usep Kasman Yulistiana Yulistiana Zakiah Fithah A’ini

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