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Journal : JURNAL PEMULIAAN TANAMAN HUTAN

KERAGAMAN GENETIK CENDANA PADA TEGAKAN PENGHASIL BENIH DAN TEGAKAN REHABILITASI DI NUSA TENGGARA TIMUR BERDASARKAN PENANDA ISOZIM Rini Purwiastuti; Sapto Indrioko; Eny Faridah
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2016.10.1.23-30

Abstract

This study aimed to determine the genetic diversity of sandalwood on seed production stand and rehabilitation stand in East Nusa Tenggara using isozyme genetic marker. The study was conducted using samples collected from three stands, i.e. Seed Production Area (APB) representing seed production stand, KHDTK rehabilitation stand and CSR rehabilitation stand representing rehabilitation stands. Samples of sandalwood juvenile leaves are taken randomly from each location. Sample materials taken were juvenile leaves collected randomly from each stand. There were 57 samples taken from APB, while each 25 samples were collected from rehabilitation stands of KHDTK and CSR. Isozymes analyses were carried out in the laboratory using three kinds of enzyme systems i.e Esterase (EST), Diaphorase (DIA) and Shikimate Dehydrogenase (SHD). The results showed that for genetic diversity within stands, the mean of polymorphic loci was 88.89%, with a mean number of alleles per locus 2.1667 and a mean of effective alleles 1.2103. The expected heterozygosity within stands (HS) was 0.1558, with the observed heterozygosity (HO) of 0.1402, while the mean index of fixation (FIS) was 0.1118. On genetic diversity among stands, DST, and GST values were 0.0090 and 0.0545 respectively, while total expected heterozygosity of the three stands (HT) was 0.1648. To anticipate sandalwood genetic diversity decline, it is essential to identify and record the remaining sandalwood populations, then conserve rare alleles either through in-situ or ex-situ conservation programs.
PERAN MIKORIZA PADA SEMAI BEBERAPA SUMBER BENIH MANGIUM (Acacia mangium Willd.) YANG TUMBUH PADA TANAH KERING Nur Hidayati; Eny Faridah; Sumardi Sumardi
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 1 (2015): Jurnal pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2015.9.1.13-15

Abstract

Plants that have mycorrhizal symbiosis tend to be more resistant to the drought because external hyphae of mycorrhizal will expand the surface of water uptake and it can infiltrate into capillary pores so that water uptake for the host plant need will increase. This study aimed to know the response and adaptation mechanisms of mangium in facing drought stress, to investigate mycorrhizal roles in overcoming drought stress on mangium, and to select the origin of mangium seed source that is tolerant to drought stress. The study used mangium seedlings from four different seed sources and was arranged in split plot pattern of randomized block design, with three factors. The result showed that mangium root infected by mycorrhizal decreased along with increasing level of watering intervals. Mycorrhiza increased height-diameter growth and total biomass of mangium for the entire seed source. Total biomass growth of seven month mangium was significantly influenced by the origin of seed source. The highest total biomass was derived from F-1 Wonogiri (7.14 grams) and followed by Group B (6.82 grams), Group C (6.21 g) and Group A (5.84 g). Adaptation mechanisms of mangium seedlings during drought stress were keeping the water status of the plant, improving plant roots system, and thickening the leaf blades.
INDUKSI TUNAS, MULTIPLIKASI DAN PERAKARAN Gyrinops versteegii (Gilg.) Domke SECARA IN VITRO Aziz Akbar M; Eny Faridah; Sapto Indrioko; Toni Herawan
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.452 KB) | DOI: 10.20886/jpth.2017.11.1.1-13

Abstract

Gyrinops versteegii (Gilg.) Domke is including one of superior agarwood-producing plants and naturally growing in Eastern Indonesia as Nusa Tenggara and Papua. Indonesia has been trading agarwood products both domestically and overseas which one of them is agarwood produced by G.versteegii. This study purpose was to develop an in vitro culture method for mass propagation of G. versteegii. Shoot induction conducted on MS medium supplemented with Benzyl Amino Purine (BAP) 0.7; 1.0; 1.5; and 2.0 mg/l. The shoots multiplication conducted on MS medium supplemented with the best concentration of BAP from shoot induction phase. The rooting of shoots conducted on half strength MS medium supplemented with interaction of Napthalene Acetic Acid (NAA) 0.01 mg/l with concentration of Indole-3-Butyric Acid (IBA) 1.0; 2.0; 3.0; and 4.0 mg/l. Epicotyl explant with a given concentration level of BAP 0.7 mg/l produce the highest rates number of shoots and shoot length compared to other explant respectively 4.8 shoots and 0.41 cm within 6 weeks. The best explant developments in the best medium able to promote the growth of the length and number of shoots are 0.28 shoots and 0.3 cm within 4 weeks. Explants easiest, quickest and most high sprouting ability as a factor of success in terms of multiplication is epicotyl. The combination treatment of material explant with concentration of BAP only affect to growth of shoots length. The combination treatment of NAA with concentration of IBA has no effect against root formation and growth rootlength.
KEMAMPUAN BERAKAR STEK PUCUK DARI BEBERAPA KLON Pinus merkusii KANDIDAT BOCOR GETAH Gunawan Nugrahanto; Mochamad Naiem; Sapto Indrioko; Eny Faridah; Widiyanto Widiyanto
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 2 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2019.13.2.71-83

Abstract

Pinus merkusii sap is one of Indonesia's superior NTFP products with high economic value. Increasing the sap productivity through the uses of geneticaly improved material of high resin yielder pinus, referred as “Pinus Bocor Getah” (PBG), is necessary. Vegetative propagation technique could used to obtain the high productivity of PBG in shorter times. This research aims to observe the rooting ability of PBG clones through a callus shoot cuttings technique. Of the 24 clones observed, 14 clones were taken from the hedge orchards and 10 clones were from selected tree in progeny test. Two experimental design used are completely randomized block designs and completely random design. Observation on the rooting ability includes parameters of the success rate of rooted, height increase, number and length of primary roots, number and length of secondary roots, root volume, root biomass and shoot and root ratio. The results showed that all tested clones showed ability to perform rooted shoot cutting, but the percentage rate of rooting varied among the clones. Amount of 71.42% clones from hedge orchard were rooted above 50% with Clone Pkp 9 and Pkp 13 as the highest percentage of rooting ( 93.75%). For the clones from progeny test, only one clone of Ppt 17 was rooted at above 50% (82.08%). The clones were significantly affected the primary root length and the number of secondary roots, especially for the clones from hedge orchard. The highest primary root length was found in Clone Pkp 3 (66.38 cm), and the highest number of secondary roots was found in Clone Pkp 14 (39). Other observed parameters were not significantly affeted by the difference of clones. The results of the study indicate that some PBG clones have the potential to be further developed massively through clonal propagation techniques.
ASOSIASI VEGETASI TANAMAN RAMBATAN KETAK (Lygodium circinatum (Burm.)Sw.) di KPHL RINJANI BARAT, PULAU LOMBOK, NUSA TENGGARA BARAT Endah Wahyuningsih; Eny Faridah; Budiadi Budiadi; Atus Syahbudin
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 2 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2019.13.2.85-94

Abstract

Ketak (Lygodium circinatum (Burm.) Sw.) is a fern plant and its growth requires other plants as a host plant for climbing of tendrils to get light. Information of vegetation association of host plant is needed, due to it is considered to have an influence on fern growth and productivity. This data and information is important to develop a strategy of ketak cultivation. This research was aimed to determine vegetation association of ketak host plant in its habitat. The study used purposive systematic sampling by survey technique based on altitude i.e. 0-249, 250-499 and 500-750 m asl. There are 10 of observation plot with dimension of 20 m x 20 m. In each plot, an analysis of vegetation association of ketak host plant to determine association between species, type of interaction and index of association between species of ketak host plant. The results showed that the vegetation association of ketak host plants in each altitude classification which had a positive and strong association are 7 pairs species in altitude of 0-249 m asl. and altitude 250 – 499 m asl., and 10 pairs species in the altitude 500 – 750 m asl.