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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Lanskap Indonesia Jurnal AGROTEKNOLOGI AGRIKA Plumula : Berkala Ilmiah Agroteknologi AGRIVITA, Journal of Agricultural Science REKAYASA UNEJ e-Proceeding Jurnal Matematika Sains dan Teknologi Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JURNAL AGROHITA Jurnal Ilmiah Hijau Cendekia Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrium Agro Bali: Agricultural Journal Jurnal Agrotropika Nusantara Science and Technology Proceedings Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI) Edu Spectrum: Journal of Multidimensional Education Jurnal Bioteknologi & Biosains Indonesia
Pangesti Nugrahani
Program Studi Agroteknologi, Fakultas Pertanian, UPN “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Other

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Pengaruh Konsentrasi dan Waktu Aplikasi Giberelin terhadap Pertumbuhan dan Hasil Tanaman Melon (Cucumis melo L.): Effect of Gibberellin Concentration and Application Timing on the Growth and Yield of Melon (Cucumis melo L.) Oktaviana Fasya, Elfira Rizki; Hidayat, Ramdan; Nugrahani, Pangesti
Agrocentrum Vol. 3 No. 2 (2025): Agrocentrum
Publisher : Agriculture Faculty - UPN "Veteran Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/agrocentrum.v3i2.87

Abstract

Melon (Cucumis melo L.) is a horticultural variety widely cultivated in Indonesia due to its sweet taste, nutritional content, and high economic value. This study aimed to determine the optimal concentration and application timing of gibberellin (GA₃) to enhance the growth and yield of Fujisawa melon plants. The research was arranged in a factorial design using a Completely Randomized Design (CRD) with two factors and four replications. The first factor was gibberellin concentration (0, 50, 100, and 150) ppm, and the second factor was application timing (pre-anthesis, anthesis, and post-anthesis stages). The results showed a significant interaction between gibberellin concentration and application timing on fruit volume. The single treatment of 100 ppm GA₃ had the best effect on plant height, harvest time, and number of fruits formed.
PENGARUH PEMBERIAN IAA DAN BAP TERHADAP WAKTU MUNCUL TUNAS TANAMAN PULE PANDAK (Rauvolfia serpentina) SECARA IN VITRO Pribadi, Nathasya Yusvie; Nugrahani, Pangesti; Makhziah, Makhziah
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.6839

Abstract

Rauvolfia serpentina merupakan tanaman obat yang memiliki berbagai manfaat farmakologis, terutama dalam mengatasi hipertensi, gangguan saraf, dan insomnia. Populasi tanaman ini semakin menurun akibat kesulitan perkecambahan dan eksploitasi bagian akar sebagai bahan obat. Penelitian ini bertujuan mengoptimalkan multiplikasi tunas pule pandak melalui kultur jaringan dengan kombinasi zat pengatur tumbuh (ZPT) indole-3-acetic acid (IAA) dan 6-benzylaminopurine (BAP). Rancangan yang dipergunakan adalah rancangan acak lengkap (RAL) faktorial yang terdiri dari dua faktor perlakuan yaitu IAA (0.0, 0.5, 1.0, dan 1.5 mg/l) dan BAP (0, 1, 2, dan 3 mg/l). Parameter yang diamati meliputi persentase hidup, waktu muncul tunas, dan waktu muncul daun. Hasil penelitian menunjukkan bahwa terdapat interaksi nyata pada kombinasi antara perlakuan IAA dan BAP pada waktu muncul daun. Kombinasi perlakuan 1.0 mg IAA dengan 2 ppm + 3 ppm BAP serta 1.5 ppm IAA + 1 ppm , 2 ppm dan 3 ppm BAP mengakibatkan daun muncul paling cepat, berturut-turut sebesar 15.33, 1567, 15.67, 16.00 dan 14.67 hari.  Sementara itu tidak terdapat interaksi nyata antara perlakuan IAA dan BAP pada parameter persentase hidup dan waktu muncul tunas. Persentase hidup akibat perlakuan 0.5 mg/l dan 1.5 mg/l lebih besar dibanding tanpa IAA, berturut-turut sebesar 86.22%, 83.33% dan 69.44%, sementara semua pemberian konsentrasi BAP mengakibatkan persentase hidup yang lebih tinggi dibanding tanpa BAP. Waktu muncul tunas tercepat diakibatkan perlakuan tunggal 1.0 ppm IAA, yaitu 16.66 hari, juga diakibatkan perlakuan 2 dan 3 ppm BAP, selama 16.00 dan 16.00 hari.
PENGARUH KONSENTRASI ZnNPs DAN ZAT PENGATUR TUMBUH TERHADAP PERTUMBUHAN EKSPLAN ANGGREK DENDROBIUM Wardhana , Arya Wira; Nugrahani, Pangesti; Sutini, Sutini
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.7387

Abstract

Salah satu upaya meningkatkan kualitas planlet Dendrobium adalah dengan menambahkan unsur mikro seperti ZnNPs ke dalam media kultur jaringan. Hal ini dapat mengatasi permasalahan yang timbul akibat batang yang berongga dan mudah patah, karena ZnNPs berperan mengoptimalkan aktivitas enzim dan sintesis lignin yang memperkuat dinding sel dan meningkatkan kepadatan jaringan batang. Penelitian ini bertujuan mengetahui pengaruh konsentrasi ZnNPs (Zinc Nanopartikel) terhadap morfogenesis dan pertumbuhan anggrek Dendrobium. Parameter yang diamati meliputi waktu muncul tunas, waktu muncul daun. Hasil penelitian menunjukkan bahwa Kombinasi antara ZnNPs dan zat pengatur tumbuh memberikan respon yang bervariasi terhadap parameter waktu muncul tunas, waktu muncul daun, waktu muncul akar, jumlah tunas dan jumlah daun/planlet. Kombinasi perlakuan berpengaruh nyata terhadap parameter waktu muncul tunas, waktu muncul daun, waktu muncul akar dan jumlah tunas.
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
The Effect of Zn Nanoparticle Concentration and the Comparison of Growth Regulator Concentration on the Growth of Cavendish Banana Explants (Musa acuminata) Nurmala Putri Rukmana, Sarah; Nugrahani, Pangesti; Utomo Pribadi, Didik
Edu Spectrum: Journal of Multidimensional Education Vol. 1 No. 2 (2024): Edu Spectrum: Journal of Multidimensional Education
Publisher : Pusat Riset dan Inovasi Nasional Mabadi Iqtishad Al Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70063/eduspectrum.v1i2.46

Abstract

Cavendish banana (Musa acuminata L.) propagation through conventional methods requires a considerable amount of time. Tissue culture techniques offer a more efficient alternative, enabling the production of plantlets with superior quality and quantity in a shorter period. The application of Zn nanoparticles has been shown to reduce media contamination and act as a biostimulant, enhancing propagation efficiency in tissue culture. Auxins, such as Naphthalene Acetic Acid (NAA), facilitate cell development and root induction, while cytokinins, including 6-Benzyl Amino Purine (BAP), are effective in stimulating cell division, morphogenesis, and shoot formation with improved stability and resistance to oxidation. This study was conducted from February to June 2023 at the Biotechnology Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional "Veteran" Jawa Timur. The research utilized a Completely Randomized Design (CRD) with two factors: the concentration of Zn nanoparticles and the ratio of ZPT concentrations. The results indicated that the best treatment for multiplication was obtained with 50 ppm Zn nanoparticles, resulting in the fastest shoot emergence at 24,63 days and the highest number of shoots per plantlet at 2,58 shoots. Furthermore, the treatment with a ZPT concentration ratio of NAA + BAP (0:0) produced the highest average number of plantlet leaves, with 5,78 leaves per plantlet.
Synergystic Role of Cocopeat Matrix and Foliar Nutrient Type on Aclimatization Success of Micropropagated Dendrobium Seedlings Luthfi, Baharuddin Mohammad; Hidayat, Ramdan; Nugrahani, Pangesti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8174

Abstract

Background: Dendrobium orchids have high economic value (in 2020 generated an export value of US$61.87 thousand), yet micropropagated seedlings commonly exhibit slow growth and high mortality during the acclimatization stage. This problem is largely associated with the use of unsuitable planting media and inadequate nutrient supply, which limit seedling adaptation and survival after tissue culture. This study aims to determine the best combination of planting media and foliar fertilizer types for the growth of Dendrobium orchid seedlings during the acclimatization stage. Methodology: The study was conducted in Penambangan Village, Semanding District, Tuban Regency, East Java Province from November 2024 to February 2025 using a Completely Randomized Design (CRD) with 9 treatment combinations and 4 replications. The first factor was the treatment of planting media types (M1: Black Moss, M2: Rice Husk Charcoal, M3: Cocopeat), and the second factor was the type of foliar fertilizer (P1: Gandasil D, P2: Gaviota 63, P3: Grow Quick LB). The observed research variables included the percentage of live seedlings, plant height, leaf area, seedling growth rate, number of shoots, and number of roots. Findings: The results showed that the use of cocopeat planting media resulted in the highest percentage of seedling survival at 88.33%. The combination of cocopeat planting media and Grow Quick LB leaf fertilizer produced the best relative growth rate (RGR) at 8-12 WAP of 41.22 mg/month.This research produces the best combination of planting media and foliar fertilizer for orchid seedlings at the acclimatization stage. Contribution: The findings contribute to a more systematic understanding of how substrate physical properties and foliar nutrient supply interact to influence survival and growth performance during orchid acclimatization, offering a scientifically grounded framework for improving ex vitro propagation efficiency
The Effect of Gibberellin (GA3) Concentration on the Growth and Yield of Several Eggplant Varieties (Solanum melongena L.) Sari, Efrilia Lukita; Nugrahani, Pangesti; Djarwatiningsih
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 1 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 1 January 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.4741

Abstract

Eggplant (Solanum melongena L.) is an important horticultural crop with high economic value and is widely cultivated in various regions due to increasing demand. This study aimed to determine the effects of gibberellin (GA3) concentration and eggplant varieties on the growth and yield of eggplant (Solanum melongena L.). The research was conducted in Kolomayan Village, Wonodadi District, Blitar Regency, from February to May 2025. A Completely Randomized Design (CRD) with a factorial arrangement of two factors was used. The first factor was gibberellin concentration at 0 ppm, 100 ppm, 200 ppm, and 300 ppm. The second factor was the eggplant variety, including Hitavi, Mustang, Pulus, and Teho 555. Parameters observed included vegetative and generative growth traits. The results showed a significant interaction between 200 ppm GA3 concentration and the Hitavi variety on total harvested fruit weight. The 200 ppm GA3 treatment alone produced the best results across all observed parameters. The Teho 555 variety excelled in plant height and leaf number, while the Hitavi variety outperformed others in flower number, total fruit number per plant, total fruit weight per plant, and fruit set.
Influence Concentration and Immersion Time, Substance Regulator Growth of IBA (Indole-3-Butyric Acid) Against Early Seedling Growth Cuttings, Plant Jasmine White (Jasminum sambac) Fajri, Ridwan; Triani, Nova; Nugrahani, Pangesti
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 2 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 2 Mei 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i2.5416

Abstract

White jasmine (Jasminum sambac (L.) Aiton) is an ornamental plant of high economic value that requires access to quality, uniform seeds. Propagation through stem cuttings often faces challenges in root formation and initial growth. This study aims to analyze the effects of Indole-3-Butyric Acid (IBA) concentration and soaking time on the initial growth of white jasmine cuttings. The study employed a factorial completely randomized design with two factors: IBA concentration (0, 50, 100, and 150 parts per million) and soaking time (30, 60, and 90 minutes). The parameters observed included shoot emergence time, shoot length, number of leaves, number of primary roots, and root fresh weight. Data were analyzed using analysis of variance at a 5% significance level. The results showed a significant interaction between concentration and soaking time for all parameters. The combination of 100 ppm IBA with a 60-minute soaking time resulted in the fastest shoot emergence (11.27 days), the longest shoot length at eight weeks after planting (11.27 cm), the highest number of leaves (12.60), the greatest number of primary roots (12.52), and the largest fresh root weight (1.06 g). Treatments without growth regulators showed the lowest values for most parameters. Thus, a concentration of 100 ppm with a 60-minute soaking time was the most effective combination for enhancing the initial growth of white jasmine cuttings. Applying this treatment has the potential to improve seedling quality and uniformity, supporting the development of white jasmine cultivation.
Phenological Stage Application of Gibberellin to Enhance Fruit Quality of Melon (Cucumis melo ‘Fujisawa’) under NFT Hydroponic System Hidayat, Ramdan; Fasya, Elfira Rizki Oktaviana; Nugrahani, Pangesti; Zakiyah, Nur Meili
AGRIVITA Journal of Agricultural Science Vol 48, No 2 (2026): IN PRESS
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.5140

Abstract

National melon production remains suboptimal due to the crop’s sensitivity to the environment and lack of management practices. This study analyzed the interaction between GA₃ concentration (0, 50, 100, 150 ppm) and application timing (pre-anthesis, anthesis, post-anthesis) on growth, yield, and fruit quality of Fujisawa melon in an NFT hydroponic system. Significant concentration × timing interactions (p<0.05) optimized fruit traits at 100 ppm + anthesis: weight increased by 0.49 kg (24.1%) to 2.03 kg, flesh thickness by 1.33 cm (25.7%) to 5.17 cm, diameter by 1.79 cm (10.9%) to 16.38 cm, and volume by 413 cm³ (21.2%) to 1,950 cm³ vs. control. Regardless of timing, 100 ppm GA₃ accelerated flowering (2.71 days) and harvest (3.87 days) vs. control and increased total soluble solids by 2.33 to 16.33ºBrix. Pre-anthesis application enhanced plant length by 8.57–9.41 cm and hastened flowering by 0.87–0.99 days compared to other timings. Leaf number and fruit cavity were unaffected by either treatment (p>0.05). The regression for fruit traits at anthesis (R²=0.74–0.85) confirmed optimum concentrations of 67.50–138.50 ppm; ≥150 ppm diminished benefits. These findings provide recommendations: 100 ppm GA₃ at anthesis maximizes yield components, 75 ppm at flexible times increases sweetness levels.
Co-Authors Agus Sulistyono Ayu Setianingrum Berlina Intan Pratiwi Dewanti, Felicitas Deru Didik Utomo Pribadi Didik Utomo Pribadi Djarwatiningsih Dwi Iriyani Dwilyana, Lisa Elly Syafriani Fadila Suryandika Fajri, Ridwan Fasya, Elfira Rizki Oktaviana Fitriya Pebriana Hadi Suhardjono Holyness Nurdin Singadimedja Iis Purnamawati Kasana, Adinda Fany Kezia Putri Kirana Sekar Ayunda Kusumaningrum, Nora Augustien Larrisa, Damasa Ines Liastyn Dwika Putri Liastyn Dwika Putri Luthfi, Baharuddin Mohammad Makhziah Maroeto Maroeto Maryam Melinda Dwi Safitri Nita Anggraeni Nora Augustien Nora Augustien K. Nora Augustien Kusumaningrum Nova Triani Nur Izza, Siti Nurmala Putri Rukmana, Sarah Octavia Zuhrotun Nafiah Oktaviana Fasya, Elfira Rizki Penta SURYAMINARSIH Pikir, Juli Santoso Pratiwi, An nisa Eka Pribadi, Nathasya Yusvie Prismaya Andarema Putri, Aura Nabita Ramdan Hidayat Rifdah Nur Safitri Rr Khoirunnisa Asyahidah Safirah Mahmuda Sari, Efrilia Lukita Sri Wiyatiningsih Sutini Sutini Sutini, Sutini Sylvia Hasna Salsabila Utomo Pribadi, Didik Wardhana , Arya Wira Widiwurjani Windriyanti, Wiwin Zakiyah, Nur Meili