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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Lanskap Indonesia Jurnal AGROTEKNOLOGI AGRIKA Plumula : Berkala Ilmiah Agroteknologi REKAYASA UNEJ e-Proceeding Jurnal Matematika Sains dan Teknologi Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JURNAL AGROHITA Jurnal Ilmiah Hijau Cendekia Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrium Agro Bali: Agricultural Journal Jurnal Agrotropika Nusantara Science and Technology Proceedings Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI) Edu Spectrum: Journal of Multidimensional Education Jurnal Bioteknologi & Biosains Indonesia
Pangesti Nugrahani
Program Studi Agroteknologi, Fakultas Pertanian, UPN “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Other

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The Effect of Dosage and Frequency of Potassium Fertilization on the Growth and Yield of White Eggplant (Solanum melongena L. var. Kania) Abdulloh, Kholid Ihsan; Hidayat, Ramdan; Nugrahani, Pangesti
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 6 (2025): December 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i6.2006-2016

Abstract

Eggplant plants have a high flower drop rate, resulting in a low fruit set. Potassium fertilizer plays an important role in plant physiology and can help reduce flower drop. This study aims to determine the effect of dosage and frequency of potassium fertilization on the growth and yield of white eggplants. The research was conducted in Mojosari District, Mojokerto Regency, East Java, from December 2023 to March 2024. This research was a factorial study with two factors arranged in a Completely Randomized Design. The first factor is potassium fertilizer dosage, which included 3 levels (3, 6, and 9 g/plant), and the second factor is fertilization frequency, which included 3 levels (2, 3, and 4 times). Results showed that the combination of potassium fertilizer dosage of 9 g/plant with potassium fertilization frequency of 4 times was the best treatment combination for fruit weight per plant, with an increase of 114% compared to the combination of potassium fertilizer dosage of 3 g/plant and potassium fertilization frequency of 2 times.
Pengaruh Konsentrasi dan Waktu Aplikasi Giberelin terhadap Pertumbuhan dan Hasil Tanaman Melon (Cucumis melo L.): Effect of Gibberellin Concentration and Application Timing on the Growth and Yield of Melon (Cucumis melo L.) Oktaviana Fasya, Elfira Rizki; Hidayat, Ramdan; Nugrahani, Pangesti
Agrocentrum Vol. 3 No. 2 (2025): Agrocentrum
Publisher : Agriculture Faculty - UPN "Veteran Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/agrocentrum.v3i2.87

Abstract

Melon (Cucumis melo L.) is a horticultural variety widely cultivated in Indonesia due to its sweet taste, nutritional content, and high economic value. This study aimed to determine the optimal concentration and application timing of gibberellin (GA₃) to enhance the growth and yield of Fujisawa melon plants. The research was arranged in a factorial design using a Completely Randomized Design (CRD) with two factors and four replications. The first factor was gibberellin concentration (0, 50, 100, and 150) ppm, and the second factor was application timing (pre-anthesis, anthesis, and post-anthesis stages). The results showed a significant interaction between gibberellin concentration and application timing on fruit volume. The single treatment of 100 ppm GA₃ had the best effect on plant height, harvest time, and number of fruits formed.
PENGARUH PEMBERIAN IAA DAN BAP TERHADAP WAKTU MUNCUL TUNAS TANAMAN PULE PANDAK (Rauvolfia serpentina) SECARA IN VITRO Pribadi, Nathasya Yusvie; Nugrahani, Pangesti; Makhziah, Makhziah
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.6839

Abstract

Rauvolfia serpentina merupakan tanaman obat yang memiliki berbagai manfaat farmakologis, terutama dalam mengatasi hipertensi, gangguan saraf, dan insomnia. Populasi tanaman ini semakin menurun akibat kesulitan perkecambahan dan eksploitasi bagian akar sebagai bahan obat. Penelitian ini bertujuan mengoptimalkan multiplikasi tunas pule pandak melalui kultur jaringan dengan kombinasi zat pengatur tumbuh (ZPT) indole-3-acetic acid (IAA) dan 6-benzylaminopurine (BAP). Rancangan yang dipergunakan adalah rancangan acak lengkap (RAL) faktorial yang terdiri dari dua faktor perlakuan yaitu IAA (0.0, 0.5, 1.0, dan 1.5 mg/l) dan BAP (0, 1, 2, dan 3 mg/l). Parameter yang diamati meliputi persentase hidup, waktu muncul tunas, dan waktu muncul daun. Hasil penelitian menunjukkan bahwa terdapat interaksi nyata pada kombinasi antara perlakuan IAA dan BAP pada waktu muncul daun. Kombinasi perlakuan 1.0 mg IAA dengan 2 ppm + 3 ppm BAP serta 1.5 ppm IAA + 1 ppm , 2 ppm dan 3 ppm BAP mengakibatkan daun muncul paling cepat, berturut-turut sebesar 15.33, 1567, 15.67, 16.00 dan 14.67 hari.  Sementara itu tidak terdapat interaksi nyata antara perlakuan IAA dan BAP pada parameter persentase hidup dan waktu muncul tunas. Persentase hidup akibat perlakuan 0.5 mg/l dan 1.5 mg/l lebih besar dibanding tanpa IAA, berturut-turut sebesar 86.22%, 83.33% dan 69.44%, sementara semua pemberian konsentrasi BAP mengakibatkan persentase hidup yang lebih tinggi dibanding tanpa BAP. Waktu muncul tunas tercepat diakibatkan perlakuan tunggal 1.0 ppm IAA, yaitu 16.66 hari, juga diakibatkan perlakuan 2 dan 3 ppm BAP, selama 16.00 dan 16.00 hari.
PENGARUH KONSENTRASI ZnNPs DAN ZAT PENGATUR TUMBUH TERHADAP PERTUMBUHAN EKSPLAN ANGGREK DENDROBIUM Wardhana , Arya Wira; Nugrahani, Pangesti; Sutini, Sutini
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.7387

Abstract

Salah satu upaya meningkatkan kualitas planlet Dendrobium adalah dengan menambahkan unsur mikro seperti ZnNPs ke dalam media kultur jaringan. Hal ini dapat mengatasi permasalahan yang timbul akibat batang yang berongga dan mudah patah, karena ZnNPs berperan mengoptimalkan aktivitas enzim dan sintesis lignin yang memperkuat dinding sel dan meningkatkan kepadatan jaringan batang. Penelitian ini bertujuan mengetahui pengaruh konsentrasi ZnNPs (Zinc Nanopartikel) terhadap morfogenesis dan pertumbuhan anggrek Dendrobium. Parameter yang diamati meliputi waktu muncul tunas, waktu muncul daun. Hasil penelitian menunjukkan bahwa Kombinasi antara ZnNPs dan zat pengatur tumbuh memberikan respon yang bervariasi terhadap parameter waktu muncul tunas, waktu muncul daun, waktu muncul akar, jumlah tunas dan jumlah daun/planlet. Kombinasi perlakuan berpengaruh nyata terhadap parameter waktu muncul tunas, waktu muncul daun, waktu muncul akar dan jumlah tunas.
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
The Effect of Zn Nanoparticle Concentration and the Comparison of Growth Regulator Concentration on the Growth of Cavendish Banana Explants (Musa acuminata) Nurmala Putri Rukmana, Sarah; Nugrahani, Pangesti; Utomo Pribadi, Didik
Edu Spectrum: Journal of Multidimensional Education Vol. 1 No. 2 (2024): Edu Spectrum: Journal of Multidimensional Education
Publisher : Pusat Riset dan Inovasi Nasional Mabadi Iqtishad Al Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70063/eduspectrum.v1i2.46

Abstract

Cavendish banana (Musa acuminata L.) propagation through conventional methods requires a considerable amount of time. Tissue culture techniques offer a more efficient alternative, enabling the production of plantlets with superior quality and quantity in a shorter period. The application of Zn nanoparticles has been shown to reduce media contamination and act as a biostimulant, enhancing propagation efficiency in tissue culture. Auxins, such as Naphthalene Acetic Acid (NAA), facilitate cell development and root induction, while cytokinins, including 6-Benzyl Amino Purine (BAP), are effective in stimulating cell division, morphogenesis, and shoot formation with improved stability and resistance to oxidation. This study was conducted from February to June 2023 at the Biotechnology Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional "Veteran" Jawa Timur. The research utilized a Completely Randomized Design (CRD) with two factors: the concentration of Zn nanoparticles and the ratio of ZPT concentrations. The results indicated that the best treatment for multiplication was obtained with 50 ppm Zn nanoparticles, resulting in the fastest shoot emergence at 24,63 days and the highest number of shoots per plantlet at 2,58 shoots. Furthermore, the treatment with a ZPT concentration ratio of NAA + BAP (0:0) produced the highest average number of plantlet leaves, with 5,78 leaves per plantlet.
Synergystic Role of Cocopeat Matrix and Foliar Nutrient Type on Aclimatization Success of Micropropagated Dendrobium Seedlings Luthfi, Baharuddin Mohammad; Hidayat, Ramdan; Nugrahani, Pangesti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8174

Abstract

Background: Dendrobium orchids have high economic value (in 2020 generated an export value of US$61.87 thousand), yet micropropagated seedlings commonly exhibit slow growth and high mortality during the acclimatization stage. This problem is largely associated with the use of unsuitable planting media and inadequate nutrient supply, which limit seedling adaptation and survival after tissue culture. This study aims to determine the best combination of planting media and foliar fertilizer types for the growth of Dendrobium orchid seedlings during the acclimatization stage. Methodology: The study was conducted in Penambangan Village, Semanding District, Tuban Regency, East Java Province from November 2024 to February 2025 using a Completely Randomized Design (CRD) with 9 treatment combinations and 4 replications. The first factor was the treatment of planting media types (M1: Black Moss, M2: Rice Husk Charcoal, M3: Cocopeat), and the second factor was the type of foliar fertilizer (P1: Gandasil D, P2: Gaviota 63, P3: Grow Quick LB). The observed research variables included the percentage of live seedlings, plant height, leaf area, seedling growth rate, number of shoots, and number of roots. Findings: The results showed that the use of cocopeat planting media resulted in the highest percentage of seedling survival at 88.33%. The combination of cocopeat planting media and Grow Quick LB leaf fertilizer produced the best relative growth rate (RGR) at 8-12 WAP of 41.22 mg/month.This research produces the best combination of planting media and foliar fertilizer for orchid seedlings at the acclimatization stage. Contribution: The findings contribute to a more systematic understanding of how substrate physical properties and foliar nutrient supply interact to influence survival and growth performance during orchid acclimatization, offering a scientifically grounded framework for improving ex vitro propagation efficiency
Co-Authors Abdulloh, Kholid Ihsan Agus Sulistyono Ayu Setianingrum Berlina Intan Pratiwi Dewanti, Felicitas Deru Didik Utomo Pribadi Didik Utomo Pribadi Dwi Iriyani Dwilyana, Lisa Elly Syafriani Fadila Suryandika Fitriya Pebriana Hadi Suhardjono Holyness Nurdin Singadimedja Iis Purnamawati Kasana, Adinda Fany Kezia Putri Kirana Sekar Ayunda Kusumaningrum, Nora Augustien Larrisa, Damasa Ines Liastyn Dwika Putri Liastyn Dwika Putri Luthfi, Baharuddin Mohammad Makhziah Maroeto Maroeto Maryam Melinda Dwi Safitri Nita Anggraeni Nora Augustien Nora Augustien K. Nora Augustien Kusumaningrum Nova Triani Nur Izza, Siti Nurmala Putri Rukmana, Sarah Octavia Zuhrotun Nafiah Oktaviana Fasya, Elfira Rizki Penta SURYAMINARSIH Pikir, Juli Santoso Pratiwi, An nisa Eka Pribadi, Nathasya Yusvie Prismaya Andarema Putri, Aura Nabita Ramdan Hidayat Rifdah Nur Safitri Rr Khoirunnisa Asyahidah Safirah Mahmuda Sri Wiyatiningsih Sutini Sutini Sutini, Sutini Sylvia Hasna Salsabila Utomo Pribadi, Didik Wardhana , Arya Wira Widiwurjani Windriyanti, Wiwin