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All Journal Jurnal Hortikultura Indonesia (JHI) Jurnal Lanskap Indonesia Jurnal AGROTEKNOLOGI AGRIKA Plumula : Berkala Ilmiah Agroteknologi AGRIVITA, Journal of Agricultural Science REKAYASA UNEJ e-Proceeding Jurnal Matematika Sains dan Teknologi Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JURNAL AGROHITA Jurnal Ilmiah Hijau Cendekia Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrium Agro Bali: Agricultural Journal Jurnal Agrotropika Nusantara Science and Technology Proceedings Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI) Edu Spectrum: Journal of Multidimensional Education Jurnal Bioteknologi & Biosains Indonesia
Pangesti Nugrahani
Program Studi Agroteknologi, Fakultas Pertanian, UPN “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Other

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PENGARUH PEMBERIAN IAA DAN BAP TERHADAP WAKTU MUNCUL TUNAS TANAMAN PULE PANDAK (Rauvolfia serpentina) SECARA IN VITRO Pribadi, Nathasya Yusvie; Nugrahani, Pangesti; Makhziah, Makhziah
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.6839

Abstract

Rauvolfia serpentina merupakan tanaman obat yang memiliki berbagai manfaat farmakologis, terutama dalam mengatasi hipertensi, gangguan saraf, dan insomnia. Populasi tanaman ini semakin menurun akibat kesulitan perkecambahan dan eksploitasi bagian akar sebagai bahan obat. Penelitian ini bertujuan mengoptimalkan multiplikasi tunas pule pandak melalui kultur jaringan dengan kombinasi zat pengatur tumbuh (ZPT) indole-3-acetic acid (IAA) dan 6-benzylaminopurine (BAP). Rancangan yang dipergunakan adalah rancangan acak lengkap (RAL) faktorial yang terdiri dari dua faktor perlakuan yaitu IAA (0.0, 0.5, 1.0, dan 1.5 mg/l) dan BAP (0, 1, 2, dan 3 mg/l). Parameter yang diamati meliputi persentase hidup, waktu muncul tunas, dan waktu muncul daun. Hasil penelitian menunjukkan bahwa terdapat interaksi nyata pada kombinasi antara perlakuan IAA dan BAP pada waktu muncul daun. Kombinasi perlakuan 1.0 mg IAA dengan 2 ppm + 3 ppm BAP serta 1.5 ppm IAA + 1 ppm , 2 ppm dan 3 ppm BAP mengakibatkan daun muncul paling cepat, berturut-turut sebesar 15.33, 1567, 15.67, 16.00 dan 14.67 hari.  Sementara itu tidak terdapat interaksi nyata antara perlakuan IAA dan BAP pada parameter persentase hidup dan waktu muncul tunas. Persentase hidup akibat perlakuan 0.5 mg/l dan 1.5 mg/l lebih besar dibanding tanpa IAA, berturut-turut sebesar 86.22%, 83.33% dan 69.44%, sementara semua pemberian konsentrasi BAP mengakibatkan persentase hidup yang lebih tinggi dibanding tanpa BAP. Waktu muncul tunas tercepat diakibatkan perlakuan tunggal 1.0 ppm IAA, yaitu 16.66 hari, juga diakibatkan perlakuan 2 dan 3 ppm BAP, selama 16.00 dan 16.00 hari.
PENGARUH KONSENTRASI ZnNPs DAN ZAT PENGATUR TUMBUH TERHADAP PERTUMBUHAN EKSPLAN ANGGREK DENDROBIUM Wardhana , Arya Wira; Nugrahani, Pangesti; Sutini, Sutini
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.7387

Abstract

Salah satu upaya meningkatkan kualitas planlet Dendrobium adalah dengan menambahkan unsur mikro seperti ZnNPs ke dalam media kultur jaringan. Hal ini dapat mengatasi permasalahan yang timbul akibat batang yang berongga dan mudah patah, karena ZnNPs berperan mengoptimalkan aktivitas enzim dan sintesis lignin yang memperkuat dinding sel dan meningkatkan kepadatan jaringan batang. Penelitian ini bertujuan mengetahui pengaruh konsentrasi ZnNPs (Zinc Nanopartikel) terhadap morfogenesis dan pertumbuhan anggrek Dendrobium. Parameter yang diamati meliputi waktu muncul tunas, waktu muncul daun. Hasil penelitian menunjukkan bahwa Kombinasi antara ZnNPs dan zat pengatur tumbuh memberikan respon yang bervariasi terhadap parameter waktu muncul tunas, waktu muncul daun, waktu muncul akar, jumlah tunas dan jumlah daun/planlet. Kombinasi perlakuan berpengaruh nyata terhadap parameter waktu muncul tunas, waktu muncul daun, waktu muncul akar dan jumlah tunas.
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
The Effect of Zn Nanoparticle Concentration and the Comparison of Growth Regulator Concentration on the Growth of Cavendish Banana Explants (Musa acuminata) Nurmala Putri Rukmana, Sarah; Nugrahani, Pangesti; Utomo Pribadi, Didik
Edu Spectrum: Journal of Multidimensional Education Vol. 1 No. 2 (2024): Edu Spectrum: Journal of Multidimensional Education
Publisher : Pusat Riset dan Inovasi Nasional Mabadi Iqtishad Al Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70063/eduspectrum.v1i2.46

Abstract

Cavendish banana (Musa acuminata L.) propagation through conventional methods requires a considerable amount of time. Tissue culture techniques offer a more efficient alternative, enabling the production of plantlets with superior quality and quantity in a shorter period. The application of Zn nanoparticles has been shown to reduce media contamination and act as a biostimulant, enhancing propagation efficiency in tissue culture. Auxins, such as Naphthalene Acetic Acid (NAA), facilitate cell development and root induction, while cytokinins, including 6-Benzyl Amino Purine (BAP), are effective in stimulating cell division, morphogenesis, and shoot formation with improved stability and resistance to oxidation. This study was conducted from February to June 2023 at the Biotechnology Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional "Veteran" Jawa Timur. The research utilized a Completely Randomized Design (CRD) with two factors: the concentration of Zn nanoparticles and the ratio of ZPT concentrations. The results indicated that the best treatment for multiplication was obtained with 50 ppm Zn nanoparticles, resulting in the fastest shoot emergence at 24,63 days and the highest number of shoots per plantlet at 2,58 shoots. Furthermore, the treatment with a ZPT concentration ratio of NAA + BAP (0:0) produced the highest average number of plantlet leaves, with 5,78 leaves per plantlet.
Phenological Stage Application of Gibberellin to Enhance Fruit Quality of Melon (Cucumis melo ‘Fujisawa’) under NFT Hydroponic System Hidayat, Ramdan; Fasya, Elfira Rizki Oktaviana; Nugrahani, Pangesti; Zakiyah, Nur Meili
AGRIVITA Journal of Agricultural Science Vol 48, No 2 (2026)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.5140

Abstract

National melon production remains suboptimal due to the crop’s sensitivity to the environment and lack of management practices. This study analyzed the interaction between GA₃ concentration (0, 50, 100, 150 ppm) and application timing (pre-anthesis, anthesis, post-anthesis) on growth, yield, and fruit quality of Fujisawa melon in an NFT hydroponic system. Significant concentration × timing interactions (p<0.05) optimized fruit traits at 100 ppm + anthesis: weight increased by 0.49 kg (24.1%) to 2.03 kg, flesh thickness by 1.33 cm (25.7%) to 5.17 cm, diameter by 1.79 cm (10.9%) to 16.38 cm, and volume by 413 cm³ (21.2%) to 1,950 cm³ vs. control. Regardless of timing, 100 ppm GA₃ accelerated flowering (2.71 days) and harvest (3.87 days) vs. control and increased total soluble solids by 2.33 to 16.33ºBrix. Pre-anthesis application enhanced plant length by 8.57–9.41 cm and hastened flowering by 0.87–0.99 days compared to other timings. Leaf number and fruit cavity were unaffected by either treatment (p>0.05). The regression for fruit traits at anthesis (R²=0.74–0.85) confirmed optimum concentrations of 67.50–138.50 ppm; ≥150 ppm diminished benefits. These findings provide recommendations: 100 ppm GA₃ at anthesis maximizes yield components, 75 ppm at flexible times increases sweetness levels.
Effectiveness of Seed Priming on Vigor and Viability of Moringa Seeds (Moringa oleifera L.) Nela Octavia Sari; Nova Triani; Pangesti Nugrahani
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 3 (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 7 No. 3 September 2025
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v7i3.4383

Abstract

Moringa is often referred to as the "miracle tree" due to its rich nutrient content and medicinal properties. The increasing demand for moringa seeds has highlighted the need for effective propagation methods; however, germination remains limited by the hard seed coats and prolonged dormancy periods. This gap underscores the importance of studying moringa, particularly in efforts to enhance germination success and initial growth. This study aims to evaluate the effects of various seed priming materials, soaking durations, and the interactions between different types of seed priming materials and soaking times on the vigor and viability of moringa seeds. The research was conducted in the Biotechnology Laboratory and greenhouse at the Faculty of Agriculture, National Development University "Veteran" East Java. This study employed a Completely Randomized Design (CRD) with a factorial arrangement of two factors, comprising fifteen combinations and three replications. The first factor is the type of seed priming material (S), which includes Aqua Destillata, seaweed extract, coconut water, PEG 6000, and KNO3. The second factor is the soaking time for the priming solution (L), which consists of 12 hours, 24 hours, and 36 hours. Observations include germinability, maximum growth potential, growth rate, vigor index, and sprout weight. The combination treatment of PEG 6000 as the seed priming material with a soaking time of 12 hours yielded the highest average values for germination and maximum growth potential. A single treatment using PEG 6000 resulted in the highest average vigor index. Additionally, a single treatment with a soaking duration of 12 hours also produced the highest average vigor index. The results of this study can be recommended as optimal treatments to enhance the germination capacity and vigor of Moringa seeds.
Effectiveness of NPK Fertilizer and Gibberellic Acid (GA₃) Plant Growth Regulator on the Growth and Yield of Long Bean (Vigna sinensis L.) Inne Martina Puspita Sari; Pangesti Nugrahani; Puji Lestari Tarigan
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 3 (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 7 No. 3 September 2025
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v7i3.4414

Abstract

Long bean (Vigna sinensis L.) is a valuable vegetable commodity recognized for its high nutritional value; however, its production in Indonesia has declined. This decrease can be attributed to improper cultivation techniques. To enhance production, strategies such as NPK fertilization and the application of growth regulators, specifically gibberellic acid (GA3), can be employed to improve yields. The combination of these two methods has the potential to improve both the growth and yield of long beans. This study employed a randomized complete block design (RCBD) with two factors, where each treatment combination was replicated three times. Data analysis was conducted using analysis of variance (ANOVA). This research aims to provide insights and recommendations regarding the most effective doses of NPK fertilizer and GA3 concentrations to enhance growth and yield. Specifically, it seeks to determine the optimal concentration of GA3 that promotes the growth and yield of long bean plants. The combination of NPK fertilizer and GA3 had a significant influence on the growth and yield of long bean plants. The treatment of NPK at 15 g/plant combined with GA3 at 100 ppm resulted in increased plant height, while NPK at 5 g/plant combined with GA3 at 100 ppm led to a higher number of fruits. Additionally, the combination of NPK at 15 g/plant and GA3 at 150 ppm significantly increased pod weight. Furthermore, the individual treatments of NPK at 15 g/plant and GA3 at concentrations ranging from 100 to 150 ppm also positively affected the growth and yield of long bean plants.
In Vitro Mutation Induction of Chrysanthemum (Chrysanthemum morifolium) Maruta Variety for Lowlands Using EMS (Ethyl Methane Sulfonate) Abigail Febby Puspo Harijono; Sukendah; Pangesti Nugrahani; Ragapadmi Purnamaningsih
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 3 (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 7 No. 3 September 2025
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v7i3.4501

Abstract

Chrysanthemums (Chrysanthemum morifolium) are among the most popular ornamental plants due to their diverse types, shapes, and colors, making them highly valued in the horticultural market. In Indonesia, chrysanthemums are typically grown in highland regions, where production and flower quality are declining due to global temperature changes. To sustain production, new varieties adapted to lowland conditions are required. This study aimed to induce mutations in the Maruta chrysanthemum variety using Ethyl Methane Sulfonate (EMS) to enhance adaptability and biodiversity. Explants were treated in vitro with EMS concentrations of 1%, 1.5%, and 2%. Quantitative traits—including survival percentage, explant height, number of shoots, leaves, and roots—were analyzed using ANOVA and Tukey's test at the 5% level. Qualitative traits, such as stem and leaf color, were assessed using the Munsell Color Chart. Results showed that more than 50% of Maruta explants survived under all treatments. However, explant height, number of leaves, and number of roots decreased as EMS concentration increased, compared with the control.
Increasing the Growth and Yield of Java Sprouts Eggplant (Solanum melongena Var. Gelatik) by Administering Different Planting Media Compositions and Rabbit Urine LOF Dosages Rifdah Nur Safitri; Didik Utomo Pribadi; Pangesti Nugrahani
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 3 (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 7 No. 3 September 2025
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v7i3.4599

Abstract

Gelatik eggplant (Solanum melongena var. Gelatik), commonly referred to as Lalap eggplant, is a variety that can be processed into a vegetable. However, its production has declined due to the inaccurate selection of planting media compositions and the inadequate application of fertilizers. Therefore, optimizing the planting media composition and applying an appropriate dosage of rabbit urine LOF are expected to improve the growth and yield of Gelatik eggplant. The purpose of this study was (1) to examine the interaction between planting media composition and rabbit urine LOF dosage, (2) to assess the effect of planting media composition, and (3) to evaluate the effect of rabbit urine LOF dosage. The experiment was conducted in Tuban, East Java, using a Randomized Block Design (RBD) with two factors. The first factor was planting media composition, consisting of four levels: (a) soil (control), (b) soil: rice husk charcoal: manure (2:1:1), (c) soil: rice husk charcoal: manure (1:2:1), and (d) soil: rice husk charcoal: manure (1:1:2). The second factor was rabbit urine LOF dosage, also with four levels: (a) NPK (control), (b) 300 ml/plant, (c) 400 ml/plant, and (d) 500 ml/plant. The results showed that the combination of soil, rice husk charcoal, and manure (1:1:2) and 300 ml/plant of rabbit urine LOF produced a significant interaction effect on the number of fruits per plant per harvest period. Furthermore, the composition of the planting media alone (soil: rice husk charcoal: manure, 1:1:2) significantly affected plant height, leaf number, fruit weight per fruit, fruit weight per plant, and total fruit weight per plant. Similarly, rabbit urine LOF dosage alone (300 ml/plant) significantly influenced plant height, number of leaves, fruit weight per fruit, fruit weight per plant, and total fruit weight per plant.
Co-Authors Abigail Febby Puspo Harijono Agus Sulistyono Ayu Setianingrum Baharuddin Mohammad Luthfi Berlina Intan Pratiwi Dewanti, Felicitas Deru Didik Utomo Pribadi Didik Utomo Pribadi Djarwatiningsih Dwi Iriyani Dwilyana, Lisa Efrilia Lukita Sari Elly Syafriani Fadila Suryandika Fasya, Elfira Rizki Oktaviana Fitriya Pebriana Hadi Suhardjono Holyness Nurdin Singadimedja Iis Purnamawati Inne Martina Puspita Sari Kasana, Adinda Fany Kezia Putri Kirana Sekar Ayunda Kusumaningrum, Nora Augustien Larrisa, Damasa Ines Liastyn Dwika Putri Liastyn Dwika Putri Makhziah Maroeto Maroeto Maryam Nela Octavia Sari Nita Anggraeni Nora Augustien Nora Augustien K. Nora Augustien Kusumaningrum Nova Triani Nur Izza, Siti Nurmala Putri Rukmana, Sarah Octavia Zuhrotun Nafiah Oktaviana Fasya, Elfira Rizki Penta SURYAMINARSIH Pikir, Juli Santoso Pratiwi, An nisa Eka Pribadi, Nathasya Yusvie Prismaya Andarema Puji Lestari Tarigan Putri, Aura Nabita Ragapadmi Purnamaningsih Ramdan Hidayat Ridwan Fajri Rifdah Nur Safitri Rifdah Nur Safitri Rr Khoirunnisa Asyahidah Safirah Mahmuda Sri Wiyatiningsih Sukendah Sutini Sutini Sutini, Sutini Sylvia Hasna Salsabila Utomo Pribadi, Didik Wardhana , Arya Wira Widiwurjani Windriyanti, Wiwin Zakiyah, Nur Meili