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IDENTIFIKASI DAN UJI POTENSI BAKTERI LIPOLITIK DARI LIMBAH SBE (SPENT BLEACHING EARTH) SEBAGAI AGEN BIOREMEDIASI Fitralia Elyza; Nuni Gofar; Munawar Munawar
Jurnal Ilmu Lingkungan Vol 13, No 1 (2015): April 2015
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.012 KB) | DOI: 10.14710/jil.13.1.12-18

Abstract

Limbah minyak kelapa sawit yang terbanyak adalah SBE (Spent Bleaching Earth), limbah ini mengandung residu minyak tinggi yang dapat mencemari lingkungan, 30% residu minyak pada limbah SBE dapat digunakan bakteri untuk pertumbuhannya, sehingga adanya bakteri mampu menjadi agen bioremediasi pencemaran SBE. Penelitian dilakukan untuk mendapatkan bakteri lipolitik sebagai agen potensial bioremediasi pada limbah SBE. Metode pengambilan sampel limbah SBE secara random sampling. Sampel tanah diambil secara acak dari beberapa titik area limbah SBE. Bakteri diisolasi dari sampel limbah SBE, kemudian dilakukan tahapan yaitu : pemurnian, seleksi, uji potensi, bakteri berpotensi mereduksi lipid dikarakterisasi dan diidentifikasi genusnya. Hasil penelitian menunjukkan bahwa Aktivitas enzim lipase yang tinggi menandakan bahwa bakteri lipolitik bekerja optimal merombak zat pencemar. Bakteri yang memiliki potensi sebagai agen bioremediasi terdiri dari genus Citrobacter (B1), Enterobacter (B2) dan Acinetobacter (B3). The most palm oil waste is SBE (Spent Bleaching Earth), this waste had many reduced lipid that got pollution for inviroments, Bacteria can use lipid from SBE as much as 30% for growed. So that consist of bacteria in SBE as a potensial agent for remediation. This study aims to obtain lipolytic bacteria as a potential agent of bioremediation. The method of sampling soil were taken at random from SBE waste, Bacteria were isolated from the SBE waste, then they were selected into steps : performed purification, selection, potential test, then characterized and identified it’s genus of potential bacteria. The results showed that the higest activity enzyme of lipolytic indicated that the lipolytic bacteria worked optimal for reduce polution. Bacteria had potential as a bioremediation agent consisting of genus Citrobacter (B1), Enterobacter (B2) and Acinetobacter (B3).
Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract Sofia Sandi; Miksusanti Miksusanti; Meisji Liana Sari; Eli Sahara; Adi Supriyadi; Nuni Gofar; Asmak Asmak
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v4.i1.15

Abstract

Antibiotics are commonly used in poultry ration, and afterward these drug residues may persist in foods. There is an alternative source of antibiotics in poultry rations, one of which is using probiotics. Probiotics are living microorganisms capable of reaching the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be originated from bacteria such as lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. LAB must be able to live in the digestive system with various pH condition. pH resistance testing of LAB was performed by introducing diluted bacteria into several buffer solutions with different pH and a predetermined incubation time, then was grown in MRS agar medium. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. The design used was a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the value of LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.
PEMANFAATAN KOTORAN KERBAU SEBAGAI PUPUK ORGANIK Nuni Gofar
Jurnal Pengabdian Sriwijaya Vol 6, No 1 (2018)
Publisher : Lembaga Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37061/jps.v6i1.6014

Abstract

Kegiatan Pengabdian  kepada Masyarakat ini dilaksanakan di Desa SejaroSakti Kecamatan Indralaya Kabupaten Ogan Ilir Sumatera Selatan. Tujuan dari kegiatan ini adalah mengupayakan masyarakat  di   desa Sejaro Sakti memanfaatkan  Kotoran ternak kerbau  sebagai  salah satu sumber alternatif pupuk organik, sehingga kekurangan pupuk  selama ini dapat diatasi. Bahan yang digunakan adalah kotoran ternak kerbau  dan hijauan rawa, dengan teknologi fermentasi/ pengomposan. Karakteristik peserta menunjukkan bahwa  peserta  partisipasi aktif  tidak hanya dalam bentuk kehadiran waktu penyuluhan dan pelatihan, tetapi aktif berkomunikasi atau berdialog mengenai  cara pembuatan pupuk organik yang berasal dari kotoran ternak kerbau. Peserta  merasa tertarik dengan teknik fermentasi/pengomposan kotoran ternak kerbau sebagai pupuk organik. Hal demikian dapat dimengerti karena pada saat sekarang harga pupuk yang sangat mahal. Dengan ditemukannya inovasi tersebut diharapkan dapat menekan biaya produksi tanaman sehingga dapat meningkatkan pendapatan masyarakat. Kesimpulan dari kegiatan ini adalah  minat peternak  desa sejaro sakti  dalam pemanfaatan kotoran ternak kerbau sebagai pupuk organik   cukup tinggi dapat meningkatkan produktivitas tanaman
Bioremediation of Spent Bleaching Earth (SBE) Wastes using Lipolitic Bacteria (Bacillus cereus) with Variation of Inoculum Volume Maria Lusia; Nuni Gofar; Hary Widjajanti
Science and Technology Indonesia Vol. 3 No. 1 (2018): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.782 KB) | DOI: 10.26554/sti.2018.3.1.35-40

Abstract

Spent bleaching earth (SBE) is a solid waste was generated from the CPO refining step into cooking oil. SBE that was discharged directly into the environment has the potential to pollute the environment, because in the SBE waste contained oil and acid residues, which are easily to be oxidized and flammable. Therefore, SBE must be processed first before being discharged into the environment. One way to manage SBE is by bioremediation. Bioremediation is a method on cleaning the environment from contaminants by using biological agents, such as bacteria, fungi etc. The bacterial isolates used in this study were Bacillus cereus. This study aims to obtain the best inoculum and to know the ability of Bacillus cereus bacteria in degrading the oil content in SBE waste. This study used Completely Randomized Design with the volume of Bacillus cereus bacteria inoculum as a treatment, consisting of 6 treatment levels of 0 mL kg-1, 25 mL kg-1, 50 mL kg-1, 75 mL kg-1, 100 mL kg-1, 125 mL kg-1. Each treatment level was repeated 3 times, so taht 18 experimental units were obtained. Observation was done once a week, in a month. Parameters observed were bacterial population, percentage of oil degradation, and oil content degradation. The best treatment result for the bacterial population was obtained at the treatment of 100 mL kg-1, at week 4 which was 7,4 x 108 cfu g-1, and for the oil degradation was obtained at 50 mL kg-1 on the treatment at week 4 as big as 90,43%.
Potential of Anabaena Azollae Extract as Antimicrobial Agent For Paddy Crop Disease Nuni Gofar; Andi Diana; Eka Setianingsih
Science and Technology Indonesia Vol. 3 No. 4 (2018): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.042 KB) | DOI: 10.26554/sti.2018.3.4.183-188

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The research objective was to test antimicrobial activity from compound produced by A. azollae which symbiosis with Azolla sp. toward microbial test of X. oryzae and Pyricularia oryzae as cause of disease on paddy crop. Sampling of Azolla which symbiosis with A. azollae was done in Azolla cultivation pond at Faculty of Agriculture, Sriwijaya University. The available Azolla was consisted of two types, i.e. Azolla pinnata and Azolla mycrophiylla. The extraction of A. azollae from Azolla leaves was done by method of Ultrasound Assisted Extraction (UAE) using ethyl ester solvent and maseration method using methanol solvent. Test media for bacteria and fungus respectively are Mueller Hilton Agar (MHA) and Sabouraud Dextrose Agar (SDA). The diameter of produced clear zone is an indication of extract’s inhibitory power toward bacteria or fungus. The different of inhibitory zone diameter is compared by using t-test. Analysis of active compounds on Anabaena azollae extract was done by using HPLC. Ethyl acetate or methanol extract of A. azollae which symbiosis with A. pinnata or A. microphylla was capable to inhibit the growth of X. oryzae bacterium and P. oryzae fungus. The dominant compounds containing within extract with probability more than 60% and area of more than 10% are consisted of phytol, hexadecanoate acid and 2-(tert-butyl)-4,6-dimethyl phenol.
MICROALGAE POTENTIAL TO DECREASING DISSOLVED AMONIA CONCENTRATION (NH3) ON LIQUID WASTE OF UREA FERTILIZER FACTORY Fadjar Sidiq Hidayahtullah; Agus Hermawan; Nuni Gofar
TECHNO: JURNAL PENELITIAN Vol 8, No 1 (2019): Techno Jurnal Penelitian
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/tk.v8i1.977

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The purpose of this research to analyzing the abilities of microalgae from various water treatment processes to improving chemical properties (pH, dissolved NH3, BOD, NO3-) the liquid waste of urea fertilizer factory. The sampling method has used the grab sampling method for a certain period. Microalgae sources are obtained from various water treatment processes and mixed with waste of water at a dose of 300 mg L-1. The results showed the source of microalgae clarifier was significantly different in reducing dissolved ammonia concentration, pH, and nitrate. Based on the results of regression analysis and correlation showed that the microalgae population was partially correlated with pH, BOD, nitrate, and dissolved ammonia of 1.61%, 87.70%, 55.38%, and 9.63%. While the concentration of dissolved ammonia significantly affected pH, BOD, nitrate, and microalgae biomass growth of 84.40%.
Exploration and characterization of exopolysaccharide-producing bacteria from soil in West Kalimantan for improving sandy soil aggregation Nasrul Harahap; Dwi Andreas Santosa; Nuni Gofar
Journal of Degraded and Mining Lands Management Vol 5, No 4 (2018)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.355 KB) | DOI: 10.15243/jdmlm.2018.054.1373

Abstract

Exopolysaccharide (EPS) is a complex mix of macro-molecular electrolyte contained in the outer cells of bacteria excreted as mucus and has a role in soil aggregation. This study aims to obtain bacteria that have a high potential for exopolysaccharide-producing bacteria. A total of 112 isolates of exopolysaccharide-producing bacteria were isolated from rubber soil rhizosphere, secondary forest, and shrubs in PT. Hutan Ketapang Industri is the result of isolation on ATCC no.14 medium. Based on the observations of a morphological colony of these isolates, most of them similarities of color and shape but only 25 colonies are different isolates were obtained based on colony morphology. However, only 10 isolates formed a thick mucus or slimy when cultured on MacConcey agar. the results show that obtained three isolates of exopolysaccharide-producing bacteria have a higher value of the dry weight i.e. isolates RB292 (7.53 mg/mL) followed by RB51 (7.55 mg/mL), and RB241 (1.75 mg/mL) with 2% sucrose. Isolates RB51 and RB292 increasing significantly soil aggregate stability at 2% dosage of organic matter with soil aggregate stability index from 30.61% to 47.87% and 45.79%. Homology of the isolates with known bacteria i.e. isolate RB51 was 98.86% homolog with Klebsiella sp. LW-13, isolate RB241 was 98.65% homolog with Klebsiella pneumonia strain DSM 30104 and isolate RB292 was 98.83% homolog with Burkholderia anthina strain MYSP113
IMPROVEMENT OF RICE GROWTH AND PRODUCTIVITY THROUGH BALANCE APPLICATION OF INORGANIC FERTILIZER AND BIOFERTILIZER IN INCEPTISOL SOIL OF LOWLAND SWAMP AREA Neni Marlina; Nuni Gofar; Abdullah Halim Perdana Kusuma Subakti; Abdull Madjid Rohim
AGRIVITA Journal of Agricultural Science Vol 36, No 1 (2014)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v36i1.300

Abstract

The objective of this study was to obtain a proper balance dose between biofertilizer and inorganic fertilizer in order to increase the growth and yield of rice in Inceptisol soil of lowland swamp origin. Biofertilizer was made by enriching straw compost with N2 interceptor bacteria, phosphate solvent bacteria and growth stimulator bacteria isolated from swamp lowland in South Sumatra. This study was conducted from November 2012 to March 2013 in a greenhouse. The design used was completely randomized design (CRD) factorial, with two treatment factors consisting of inorganic fertilizer (0,25, 50, 75 and 100% recommended dosage) and biofertilizer (0, 100, 200, 300, 400 and 500 kg.ha-1). The results showed that the best treatment in term of plant height at 8 weeks after planting (WAP), the maximum number of tillers, number of productive tillers, number of grains per panicle and weight of milled dry rice were obtained in combination of 75% inorganic fertilizer and 300 - 400 kg.ha-1biofertilizer.Keywords: biofertilizer, inorganic fertilizer, lowland rice
Eksplorasi Bakteri Penghasil Eksopolisakarida dari Rizosfer Kelapa Sawit sebagai Pemantap Agregat Tanah [Exploration of exopolysaccharide-producing bacteria from Oil Palm Rhizosphere as a Soil Aggregate stabilizer] Nuni Gofar; Diana Utama
Buletin Palma Vol 21, No 1 (2020): Juni 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v21n1.2020.22-28

Abstract

Palm oil cultivated in sandy or sandy loam with aggregate not stable and easily eroded, will lack water and nutrients. To increase sandy soil aggregation, soil aggregate stabilizing agents must be added, one of which is exopolysaccharideproducing bacteria.This study aimed to explore exopolysaccharide-producing bacteria from the oil palm rhizosphere and analyze their ability to form soil aggregates. Bacterial exploration was carried out by isolating bacteria from various age groups of oil palm rhizosphere, then isolates were selected which were able to produce the best exopolysaccharide, then tested it ability in stabilizing the soil aggregate. Bacterial selection and isolation was carried out in the Soil Biology Laboratory, Department of Land, Faculty of Agriculture, Sriwijaya University. The results showed that six isolates had the potential to produce exopolysaccharide (S1A, S3A, S3B, S4A, S4B and S4C) characterized by the presence of thick slime and produce exopolysaccharide. Based on the calculation of exopolysaccharide weight, S4C isolates from oil palm rhizosphere samples over the age of 10 years were able to produce the best exopolysaccharide weight of 44.25 g mL-1. After 2 months incubation, the soil which was inoculated with S4C bacteria had very stable aggregates (excellent physical quality) which differed significantly compared to soil aggregates without bacterial inoculation (poor physical quality).It is recommended to continue the study of the ability of S4C isolates to increase aggregation and water and nutrient retention in soils with high sand content. ABSTRAKTanaman kelapa sawit yang dibudidayakan pada tanah berpasir atau lempung berpasir dengan kemantapan agregat rendah dan mudah tererosi, akan kekurangan air dan unsur hara. Untuk meningkatkan agregasi tanah berpasir, harus ditambahkan agen pemantap agregat tanah, salah satunya adalah bakteri penghasil eksopolisakarida. Penelitian ini bertujuan untuk mengeksplorasi bakteri penghasil eksopolisakarida asal rizosfer kelapa sawit dan menguji kemampuannya dalam membentuk agregat tanah. Eksplorasi bakteri dilakukan dengan mengisolasi bakteri dari rizosfer kelapa sawit berbagai umur, kemudian dipilih isolat yang mampu menghasilkan eksopolisakarida terbaik, lalu menguji kemampuannya dalam memantapkan agregat tanah. Seleksi dan isolasi bakteri dilakukan di Laboratorim Biologi Tanah, Jurusan Tanah, Fakultas Pertanian, Universitas Sriwijaya. Hasil penelitian menunjukkan enam isolat berpotensi menghasilkan eksopolisakarida (S1A, S3A, S3B, S4A, S4B dan S4C) ditandai dengan adanya slime tebal dan menghasilkan eksopolisakarida. Berdasarkan perhitungan bobot eksopolisakarida, isolat S4C asal sampel rizosfer kelapa sawit berumur lebih dari 10 tahun mampu menghasilkan bobot eksopolisakarida terbaik yaitu 44,25 g mL-1. Setelah diinkubasi 2 bulan, tanah yang diinokulasi bakteri S4C memiliki agregat dengan kualitas fisik sangat baik yang berbeda sangat nyata dibandingkan agregat tanah tanpa diinokulasi bakteri (kualitas fisik buruk). Disarankan untuk melanjutkan kajian kemampuan isolat S4C dalam meningkatkan agregasi serta retensi air dan hara pada tanah dengan kadar pasir yang tinggi.
Perbaikan Stabilitas Agregat Tanah Pasir Berlempung Menggunakan Bakteri Pemantap Agregat dan Bahan Organik Diana Utama; Nuni Gofar; Adipati Napoleon
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) Vol 42, No 2 (2018)
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v42n2.2018.161-167

Abstract

Abstrak. Penelitian ini bertujuan untuk menganalisis stabilitas agregat tanah dengan perlakuan berbagai isolat bakteri pemantap agregat (BPA) dan bahan organik berupa kompos yang terbuat dari campuran 90% rumput Cyperus pilosus Vahl dan 10% kotoran sapi, dengan masa inkubasi yang berbeda. Taraf perlakuan terdiri dari kontrol, kombinasi isolat I, II, dan III masing – masing dikombinasikan dengan komposisi bahan organik 0%, 0.5%, dan 1%. Hasil penelitian ini menunjukkan aplikasi isolat BPA pada tanah pasir berlempung disertai pemberian bahan organik menyebabkan populasi yang lebih tinggi dibandingkan tanpa aplikasi keduanya. Klebsiella sp. LW-13 yang dikombinasi dengan 1% bahan organik dan Bukholderia anthina MYSP113 yang dikombinasi dengan berbagai taraf bahan organik (0 hingga 1%) menyebabkan agregat menjadi sangat mantap sekali pada pengamatan 60 hari setelah aplikasi. Eksopolisakarida yang dihasilkan bakteri akan mengikat partikel tanah dan membentuk agregasi. Penggunaan bakteri Bukholderia anthina MYSP113 dinilai lebih efisien dalam pemanfaatannya untuk memantapkan agregat tanah karena memiliki kemampuan terbaik untuk memantapkan agregat tanah hingga sangat mantap sekali dengan atau tanpa penambahan bahan organik pada periode 60 hari pengamatan.Abstract. This study aimed to analyze the aggregate stability of soil with sdifferent treatments of aggregate-stabilizing bacteria and organic matter (compost made of mixture of 90% Cyperus pilosus Vahl grass biomass and 10% cattle manure) at different incubation period. Treatments consisted of control, combination of three different isolate with three different composition of organic matter (0%, 0.5%, and 1%). The results showed that the application of aggregate-stabilyzing bacteria to loamy sand soil and organic matter causes a higher bacteria population than without both applications. Klebsiella sp. LW-13 combined with 1% organic matter and Bukholderia anthina MYSP113 which was combined with various levels of organic matter (0 to 1%) showed high aggregation at observation of 60 days after application. The exopolysaccharide produced by bacteria binds soil particles and forms soil aggregation. The use of Bukholderia anthina MYSP113 bacteria is considered to be efficient in its utilization to stabilize soil aggregates because it has the best ability to stabilize soil aggregates to be highly stable with or without the addition of organic matter in the 60-day observation period. 
Co-Authors Abdul Madjid Abdull Madjid Rohim Abdullah Halim Perdana Kusuma Subakti Ace Baehaki Adi Supriyadi Adipati Napoleon Agus Hermawan Albertus Fajar Irawan Albertus Fajar Irawan Ali Amran Andi Diana Andika, Gusti Aditya ANDY MULYANA Angga Prasetya Mulya Anita Desiani Anjastari, Devi ANNISA NABILA Asmak Asmak Ayuputri, Niken Bambang Suprihatin Budi Untari Buyana, Nova Tri Chairu Nisa Apriyani Dade Jubaedah Dade Jubaedah Dedik Budianta Devi Anjastari Diana Sinurat Diana Utama Diana Utama Diana Utama DWI ANDREAS SANTOSA Eka Setianingsih Eli Sahara Ermatita - Erra Kartika Erra Kartika, Erra Erwin Saputra Fadjar Sidiq Hidayahtullah Fathona Nur Muzayyadah Fauzi Yusuf Syarifuddin fitra yosi, fitra Fitralia Elyza Fitri Siti Nurul Aidil Geovani, Dite Gusti Aditya Andika Hary Widjajanti Irmeilyana Kerenila Agustin Lestari, Primastya Ayu Lestari, Primastya Ayu M Kahfi Aldi Kurnia M. Umar Harun M. Umar Harun Maria Lusia Marini Wijayanti Marsi Meisji Liana Sari Miksusanti Miksusanti Muhammad Wahyu Ilahi Muhammat Rio Halim Munandar Munandar Munawar Munawar Nasrul Harahap Neni Marlina Ni Luh Putu SR Novi Wulandari Mustika Nyayu Nurul Husna Primastya Ayu Lestari Redina An Fadhila Chaniago Renih Hayati Rodiana Nopianti Rohim, A. Madjid Rosmiah Rosmiah, Rosmiah Satria Jaya Priatna, Satria Jaya Siti Komariah Hildayanti Siti Nurul Aidil Fitri Siti Nurul Aidil Fitri Siti Nurul Aidil, Fitri Sofia Sandi Sriati Sriati Sugandi Yahdin Susilawati Tanbiyaskur Tanbiyaskur Teguh Randi Pradana Tri Putri Nur Utama, Diana Utama, Diana wahyu tri patria Widya Irmawati Wuriesyliane Wuriesyliane Yopie Moelyohadi Yopie Moelyohadi Yuli Andriani