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Pengaruh Konsentrasi Bap Pada Perkecambahan Biji Pamelo Asal Aceh Secara In-Vitro Handayani, Ira; Nazirah, Laila; Ismadi, Ismadi; Rusdi, Muhammad; Handayani, Rd. Selvy
Agrium Vol 17, No 2 (2020)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v17i2.2927

Abstract

Pamelo (Citrus  maxima  (Burm.)  Merr.) besar merupakan salah satu jenis tanaman hortikultura yang mendapat perhatian dari pemerintah untuk dikembangkan dan ditingkatkan produksinya karena memiliki prospek pemasaran yang baik. Permasalahan utama pengembangan tanaman pamelo Aceh adalah ketersediaan biji yang sangat sedikit atau bahkan sering dijumpai tanpa biji dan juga bijinya sulit sekali dikecambahkan dikecambahkan secara konvensional. Oleh karena itu untuk mengatasi permasalahan tersebut dapat dilakukan secara in vitro. Tujuan penelitian ini adalah untuk mengetahui pengaruh BAP terhadap keberhasilan perkecambahan biji pamelo lokal Aceh secara in vitro. Penelitian  dilaksanakan di Laboratorium Kultur Jaringan Tanaman Fakultas Pertanian Universitas Malikussaleh pada bulan Desember 2018 sampai dengan Februari 2019. Percobaan ini menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal. Perlakukuannya adalah konsentrasi BAP yaitu 0 dan 2 mg/L dengan 30 ulangan, sehingga didapat 60 satuan percobaan. Pada percobaan ini setiap eksplan ditanam pada botol kultur dengan jumlah 2 eksplan per botol. Data hasil pengamatan dianalisis secara statistik dengan menggunakan analisis ragam. Jika hasil uji F menunjukkan adanya pengaruh yang nyata antar perlakuan, maka analisis dilanjutkan dengan Uji DMRT (Duncan’s Multiple Range Test) pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian BAP dapat mempengaruhi pertumbuhan eksplan biji pamelo secara in vitro.  Pemberian BAP 2 mg/L pada awal menyebabkan biji lebih lambat membentuk tunas dan akar, namun pada akhir pengamatan didapatkan biji menghasilkan tunas dan daun yang lebih banyak.
Pertumbuhan dan Hasil Bawang Daun Akibat Perlakuan Pupuk Limbah Kulit Kopi dan Jarak Tanam Paiman, Paiman; Solihuddin, Mahin; Hafifah, Hafifah; Ismadi, Ismadi; Usnawiyah, Usnawiyah; Handayani, Rd. Selvy
Agrium Vol 16, No 2 (2019)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v16i2.5868

Abstract

Bawang daun adalah tanaman semusim yang memiliki nilai ekonomi yang tinggi. Petani sering mengandalkan pupuk kimia dalam membudidayakan komoditas ini, sehingga produksi bawang daun menjadi lebih kecil. Penelitian ini mencoba mengkaji penggunaan pupuk organik dengan memanfaatkan limbah kulit kopi untuk mengurangi penggunaan pupuk kimia. Jarak tanam yang sesuai merupakan salah satu upaya untuk meningkatkan produksi tanaman. Penelitian ini telah dilaksanakan di Gampong Ujung Gele, Kecamatan Bukit, Kabupaten Bener Meriah pada bulan Februari-Juli 2019. Percobaan dilakukan menggunakan Rancangan Acak Kelompok (RAK) dua faktor. Faktor pertama yaitu pupuk limbah kulit kopi menggunakan 4 taraf yaitu (P0) 0 g/tanaman, (P1) 60 g/tanaman, (P2) 90 g/tanaman, dan (P3) 120 g/tanaman. Faktor kedua yaitu  jarak tanam yang menggunakan empat taraf yaitu (J1) 10 cm x 20 cm, (J2) 15 cm x 20 cm  dan (J3) 20 cm x 20 cm. Pemberian pupuk tunggal berpengaruh terhadap semua variabel yang diamati, kecuali pertumbuhan tanaman dan hasil daun bawang. Pemberian pupuk 90 g per tanaman (P2) meningkatkan pertumbuhan dan hasil bawang hijau. Penerapan ruang tanam secara tunggal berpengaruh terhadap panjang akar, berat kering dan segar per rumpun, hasil per plot dan per hektar. Hasil penelitian menunjukkan bahwa jarak tanam terbaik yang diterapkan adalah 10 cm x 20 cm (J1). Terjadi interaksi antara pemberian pupuk kulit kopi dan jarak tanam terhadap jumlah anakan, jumlah daun dan panjang akar. Interaksi terbaik terdapat pada pemberian pupuk 90 g/tanaman dengan jarak tanam 10 cm x 20 cm (P2J1).
Keberhasilan Sambung Pucuk Durian (Durio zibethinus) Lokal Aceh Akibat Perlakuan Cara dan Lama Penyimpanan Batang Atas Liwanza, Nasrun; Muksalmina, Muksalmina; Ismadi, Ismadi; Handayani, Rd. Selvy
Agrium Vol 16, No 2 (2019)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v16i2.5869

Abstract

Sambung pucuk merupakan salah satu teknik penyambungan yang biasa digunakan pada perbanyakan tanaman durian. Kendala utama pada saat penyambungan adalah  jarak antara tempat pembibitan untuk  pengerjaan sambungan (sumber batang bawah) dan pohon induk unggul lokal Aceh (sumber batang atas).  Tempat penyambungan dan pohon induk  biasanya berjauhan, bahkan  bisa sampai berbeda pulau. Selain itu, jumlah tanaman yang akan disambungkan sangat banyak sehingga sulit diselesaikan dalam waktu satu hari.  Oleh karena itu batang atas harus dikemas kembali dan disimpan, karena tertundanya waktu penyambungan. Tujuan penelitian ini adalah untuk mengetahui pengaruh cara dan lama waktu penyimpanan batang atas terhadap keberhasilan sambung pucuk tanaman durian. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dua faktor. Faktor pertama  adalah  cara penyimpanan batang atas (P) terdiri dari 2 jenis yaitu batang atas tanpa dibungkus kertas koran (P1), dan batang atas yang dibungkus kertas koran (P2). Faktor kedua adalah lama penyimpanan batang atas (L) terdiri dari 6 taraf yaitu  batang atas langsung disambungkan (L0), disimpan satu hari (L1), disimpan dua hari (L2), disimpan tiga hari (L3), disimpan empat hari (L4), dan disimpan lima hari (L5). Hasil penelitian menunjukkan bahwa faktor cara penyimpanan batang atas (dibungkus dan tidak dibungkus koran) secara tunggal tidak memberikan pengaruh di semua peubah yang diamati. Faktor lama penyimpanan batang atas secara tunggal berpengaruh terhadap pertumbuhan bibit durian hasil sambungan. Batang atas dapat disimpan paling lama hanya 2 hari. Semakin lama batang atas disimpan sebelum disambungkan, akan menurunkan tingkat keberhasilan sambungan. Kombinasi perlakuan antara cara dan lama penyimpanan batang atas tidak memberikan pengaruh di semua peubah yang diamati.
Effect of Bap and Coconut Water on Pamelo (Citrus maxima (Burm.) Merr.) Seed Initiation in Vitro Zia, Khaliza; Nilahayati, Nilahayati; Nazirah, Laila; Handayani, Rd. Selvy; Hafifah, Hafifah
Journal of Tropical Horticulture Vol 4, No 1 (2021): April 2021
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v4i1.71

Abstract

Pomelo is a fruit commodity with high economic value and is in great demand by the public. Pomelo contains vitamins, proteins, and minerals the body needs to fulfill balanced nutrition. Aceh is one of the areas producing pomelo in Indonesia. The production of pamelo in Aceh is generally not optimal due to the limited supply of seeds that cannot be conventionally germinated. Plant tissue culture is one of the methods of propagation that can produce new plants in a relatively shorter time and large quantities. This research aims to determine the effect of natural and synthetic growth regulators on the growth of pomelo plants in vitro. The research used a factorial Randomized Block Design (RBD) of 2 factors. The first factor is the concentration of Benzyl Amino Purine (BAP) (0 mg/L, 1 mg/L, and 2 mg/L) and concentration of coconut water (0%, 15% and 30%).
In-vitro Callus Induction of Durian (Durio zibethinus Murr.) Leaves Using Kinetin and 2,4-D (Dichlorophenoxyacetic acid) Handayani, Rd. Selvy; Yunus, Ismadi; Sayuti, M.; Irawan, Endri
Journal of Tropical Horticulture Vol 2, No 2 (2019): October 2019
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1325.828 KB) | DOI: 10.33089/jthort.v2i2.23

Abstract

Durian (Durio zibethinus Murr.) is a tropical fruit grown in Southeast Asia and it has high nutritional and economic values and highly appreciated by consumers in Indonesia.  Unfortunately, there are several obstacles in planting durian in Indonesia, specifically in Aceh.  One of the abstacles is the scarcity of superior durian plants. Generally, plant conservations done conventionally through vegetative propagation.  However, this technique has disadvantage such as harming the initial plants.    Therefore, plant culture tissue has been used as a modern technique to develop durian plants. The objective of this research was to evaluate the effect of kinetin and 2,4 D on development of durian leaf explants through in-vitro bioassay. The research was conducted at Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University from January to April 2019 using Completely Randomized Design (CRD) Factorial with 2 factors observed. The first factor was Kinetin (K): 0.0, 0.1 and 0.5 ppm. The second factor was auxin 2,4 D: 0.0, 0.5 and 1.0 ppm.  The results showed that the in-vitro application of kinetin affected the induction of leaf callus.  The application of 0.5 ppm of kinetin gave the best result compared to others.  The in-vitro application of 2,4 D 0.5 ppm also possessed the best result compared to others. There was no interaction beween in-vitro application of kinetin and 2,4 D on the growth of durian leaf callus at all parameters observed.
The Effect of Limingtime and Bap Application on the Growth of Potato (Solanum tuberosum L.) Granola Varieties In Vitro Micro Sets Meutia, Indah; Handayani, Rd. Selvy; Hafifah, Hafifah; Nilahayati, Nilahayati; Nasruddin, Nasruddin
Journal of Tropical Horticulture Vol 5, No 1 (2022): April 2022
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v5i1.77

Abstract

Potato is a horticultural plant that contains carbohydrates and nutrients. However, the problem faced in potato cultivation is the difficulty of obtaining high-quality tubers. Efforts that can be made to obtain healthy potato seeds are by propagating them using tissue culture techniques. The research aimed to determine the effect of the length of irradiation and application of BAP on the growth of granola potato micro cuttings in vitro. This research was conducted using a Split Plot Design (SPD) in a Completely Randomized Design (CRD) with two factors: length of irradiation (24-hour dark and 24-hour light) as the primary plot and BAP (0, 1, 2 mg/L) as sub-plots. Each treatment was performed in five replications. The results showed that the length of irradiation affected shoot growth time, number of roots (4-10 WAP), number of leaves (1-10 WAP), and shoot length (2-4 and 10 WAP). The best treatment was found in the 24-hour light treatment (L1). BAP treatment affected the percentage of growth success (9 and 10 WAP), root growth time, number of shoots (1, 4-10 WAP), number of roots (1-10 WAP), number of leaves (4-10 WAP), and shoot length (1-10 WAP).
Potential Changes in Watermelon (Citrullus lannatus) Ploidy Treated By Colchicine Handayani, Rd. Selvy; Yusuf, Muhamad; Akmal, Ajmir
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1406.163 KB) | DOI: 10.33089/jthort.v1i1.6

Abstract

The purpose of this study was to determine the effect of colchicine on changes in ploidy watermelon. The research was conducted in Agroecotechnology laboratory Universitas Malikussaleh, Microtechnique laboratory Agronomy and Horticulture Departement, Bogor Agricultural University, and Beuringen, Murah Mulia subdistrict, North Aceh. This research used Completely Randomized Design (CRD) two factors. The first factor was watermelon seed soaking time in colchicines 0,02% ie.0, 24,36, and 48 hours. The second factor was the concentration of the colchicine solution on the sprout growth point i.e. 0, 0,1, and 0,2 %. The results showed that plants were given colchicine became to shorter and fewer number of leaves than plants without any treatment. Colchicine could increase the size of the stomata, but it did not change the shape of stomata. Plants that were given colchicine had the potential to ploidy multiplication.
Growth Response of Cymbidium Orchid Subcultures Due to the Treatment of Ms Media Concentration and Foliar Fertilizer in Vitro Arbi, Dwiki Prahdano; Nilahayati, Nilahayati; Nazirah, Laila; Handayani, Rd. Selvy; Hafifah, Hafifah
Journal of Tropical Horticulture Vol 4, No 1 (2021): April 2021
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v4i1.73

Abstract

Orchids are very popular in Indonesia and the world as ornamental plants and floral arrangements. Therefore, orchids should be maintained and cultivated as they have high economic value. Orchids can be propagated by using seeds. Orchid seeds are tiny and do not have endosperm, making growing difficult. Efforts can be made to propagate this plant by using tissue culture methods. This study was conducted to determine the effect of MS media concentration and foliar fertilizer on the growth of Cymbidium orchid subcultures in vitro. The research method used a factorial randomized block design (RBD) of two factors. The first factor is the MS media concentration with three levels, which are M0 (full of MS), M1 (½ of MS), and M2 (¼ of MS). The second factor is the concentration of foliar fertilizer, which consists of three levels: P0 (0 g/L), P1 (1 g/L), and P2 (2 g/L).
The Effect of Cutting Length and Coconut Water Concentration on the Success of Lemon Cuttings (Citrus Lemon L.) Elviana, Elviana; Handayani, Rd. Selvy; Safrizal, Safrizal; Hafifah, Hafifah; Hendrival, Hendrival
Journal of Tropical Horticulture Vol 4, No 2 (2021): October 2021
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v4i2.68

Abstract

Lemon (Citrus lemon L.) is one of the horticultural commodities used by Indonesian people in the form of juice or combined with cooking spices. The materials used for lemon cutting plants were topsoil, manure, coconut water, water, labeling paper, transparent plastic, and polybag size 12 x 17 cm. The tools used in this research were: hoe, machete, scissors, bucket, bamboo, paranet, raffia rope, meter, camera, watering can, measuring cup, scale, oven, and stationery. This research used Randomized Block Design (RBD) with a factorial pattern. The first factor was the length of the cuttings, and the second factor was the concentration of coconut water (0% (control), 25%, 50%, and 75%). It was repeated three times so that there were 36 experimental units.
The Relationship between Transpiration and Calcium Fertilization on Mangosteen (Garcinia mangostana L.) Seedlings Akmal, Ajmir; Santosa, Edi; Poerwanto, Roedhy; Yunus, Ismadi; Handayani, Rd. Selvy
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1559.113 KB) | DOI: 10.33089/jthort.v1i1.7

Abstract

The availability of nutrients in plants depends on the ability of plants to absorb nutrients from the soil. One of the processes absorbed by plants is by the presence of the transpiration process.  Good transpiration will provide enough nutrients for plants which increase the productivity and quality of mangosteen. One of the quality standards of mangosteen fruit for export quality is the free from yellow sap contamination. It can be possible that transpiration can improve the quality of mangosteen fruit by applying fertilization. In this study Ca fertilization was carried out on mangosteen seeds, where this study aims to investigate the effectiveness of Ca uptake by mangosteen plants with the level of water loss by transpiration. This research was conducted in November 2016-March 2017 at the Leuwikopo Experimental Garden, Bogor Agricultural University. Morphological observations were carried out in its Experimental Garden and the Postharvest Laboratory whileanalysis of calcium content of plant tissue was done at the Department of Agronomy and Horticulture, Bogor Agricultural University. Data obtained then were analyzed using ANOVA test. If the results were significant, Duncan Multiple Range Test (DMRT) was then tested at 0.05 probability level. The results revealed that the transpiration rate of mangosteen plants from several treatments showed a significant difference, the transpiration rate was higher, especially in fertilized plants. The size of the 12th leaf, plants treated with fertilizer have a larger size than the treatment without fertilization. Fertilizing and providing enough water can maintain the growth of the plants.