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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Soils Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati AGRIVITA, Journal of Agricultural Science Jurnal Agrotek Tropika Jurnal Agrotropika
DWI HAPSORO
The University of Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science

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PENGARUH PEMOTONGAN BIJI DAN APLIKASI GA3 TERHADAP PERKECAMBAHAN TANAMAN ALPUKAT (Persea americana Mill.) SEBAGAI UPAYA PENYEDIAAN ROOTSTOCK YANG BERADAPTASI LUAS Pratiwi, Bela Ayu; Yusnita, Yusnita; Hapsoro, Dwi; Karyanto, Agus; Ramadiana, Sri
Jurnal Agrotek Tropika Vol. 13 No. 1 (2025): JURNAL AGROTEK TROPIKA VOL 13, FEBRUARI 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i1.6802

Abstract

Batang bawah (rootstock) yang digunakan dalam perbanyakan alpukat melalui grafting umumnya berasal dari biji sehingga menghasilkan perakaran yang kuat dan dapat beradaptasi luas. Namun, biasanya beberapa biji mengalami perkecambahan dan pertumbuhan yang terhambat sehingga rootstock yang diperlukan untuk dilakukan penyambungan menjadi tidak seragam dalam pertumbuhannya. Upaya yang dapat dilakukan untuk mengatasi hal ini yaitu dengan cara pemotongan biji bagian bawah dan aplikasi zat pengatur tumbuh (GA3). Penelitian ini dilakukan bertujuan untuk mempelajari pengaruh pemotongan biji bagian bawah, perendaman biji dalam beberapa konsentrasi GA3, dan kombinasi antara pemotongan biji bagian bawah dan perendaman biji dalam beberapa konsentrasi GA3 terhadap perkecambahan dan pertumbuhan seedling alpukat yang beradaptasi luas. Percobaan ini dilaksanakan dengan rancangan acak lengkap dengan perlakuan yang disusun secara faktorial (2x3) dan tiga ulangan. Faktor pertama yaitu pemotongan biji bagian bawah yang terdiri dari dua taraf yaitu tanpa pemotongan dan pemotongan, sedangkan faktor kedua yaitu perendaman dalam beberapa konsentrasi GA3 yang terdiri dari 3 taraf yaitu 0, 250, dan 500 ppm. Data pengamatan yang diperoleh dilakukan uji F dan uji lanjut menggunakan beda nyata terkecil dengan taraf 5%. Hasil penelitian menunjukkan bahwa pemotongan biji bagian bawah mampu mempercepat tercapainya semua biji berkecambah (100%) dalam 4 minggu setelah tanam (MST) pada semua perlakuan GA3, sedangkan tanpa pemotongan biji belum semuanya berkecambah kecuali pada perlakuan aplikasi GA3 500 ppm. Perendaman biji dalam GA3 250 atau 500 ppm mempercepat perkecambahan biji dan meningkatkan pertumbuhan seedling alpukat.
Successful Grafting of Two Indonesian Clones of Piper nigrum L. with P. colubrinum Link.: Effects of IBA and NAA on Rooting and Effects of BA on Grafting Yusnita, Yusnita; Hapsoro, Dwi; Prayogi, Adi Noor; Agustiansyah, Agustiansyah; Karyanto, Agus
AGRIVITA Journal of Agricultural Science Vol 46, No 1 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i1.3899

Abstract

This study aimed to investigate the effects of auxin types and their concentrations on rooting of the rootstock cuttings and BA on grafting. First, IBA, NAA or IBA+NAA, each at 0, 1000, 2000, and 3000 ppm were used as treatments to study rooting of Piper colubrinum. In the second experiment, BA (0 and 50 ppm) was applied to P. nigrum scion clones of Natar-1 and Petaling-2, before being grafted to the rootstocks. Results showed, that all types of auxins (IBA, NAA or IBA+NAA) induced rooting and shoot growth, and the increase of auxin concentrations led to the increase of rooting. However, their effectiveness was different. NAA and IBA+NAA were superior to IBA, and at 2000 ppm, IBA+NAA resulted in the best plant growth, as indicated by higher values of both rooting and shoot growth parameters. Compared to control, BA treatment on scions resulted in higher grafting success (80% vs. 93%) for Natar-1, and (73% vs.100%) for Petaling-2 clones.  BA treatment on scions also induced more calluses in the graft union and better shoot growth. To our knowledge, this is the first report on the use of BA to increase success of grafting between P. nigrum and P. colubrinum.
Perbanyakan In Vitro Sansevieria trifasciata ‘Lorentii’: Regenerasi Tunas, Pengakaran, dan Aklimatisasi Planlet Yusnita, ,; Wahyuningsih, Triani; Sulistiana, Puji; Hapsoro, Dwi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.441 KB) | DOI: 10.24831/jai.v41i1.7080

Abstract

This research aimed to study effects of benzyladenine (BA) on in vitro shoot formation and effects of indolebutyric acid(IBA) and acclimatization media on ex vitro rooting and acclimatization of Sansevieria trifasciata ‘Lorentii’. Leaf segmentswere taken from young fully-expanded leaves, surface sterilized and cultured on Murashige and Skoog (MS) basal mediumcontaining 0.25 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) for 2 weeks, transferred to medium without growth regulatorfor 2 weeks, and then subcultured on MS medium containing BA (0, 0.5, 1 and 2 and 5 mg L-1). The results showed thatadventitious shoot regeneration occured after callus formation. The best BA concentration was 2 mg L-1, producing 4.5 shootsper explants in 3 months and 11.1 shoots per explant in 4 months. Application of 2000 ppm IBA and the use of acclimatization  medium consisting rice husk charcoal and compost (1:1) produced the highest number of primary roots, length of roots and root fresh weight. However, the ex vitro rooting did not influence the success of plantlet acclimatization, the survival rate being 96% and there were no significant difference in plant growth.Keywords: benzyladenine, ex vitro, indolebutyric acid, 2,4-dichlorophenoxyacetic acid, micropropagation, Sansevieriatrifasciata
Co-Authors , Agustiansyah , Yusnita Afriliyanti, Rizki Agus Karyanto Ainin Niswati, Ainin Bayu Lesmana, Bayu Bela Ayu Pratiwi Desi Maulida Dwi Asmono Ekawati Danial Eko Pramono Farida Hanum HAJRIAL ASWIDINNOOR Jumanto Jumanto, Jumanto Kukuh Setiawan Maera Zasari Motiq, Feria Wirana Nugroho, Rizki Bagus Prayogi, Adi Noor Puji Sulistiana Rafika, Eni Rahmadyah Hamiranti Ramadiana, Sri Rugayah Rugayah RUSMILAH SUSENO Sri Yusnaini Sudarsono Triani Wahyuningsih Ulumudin, Ade Utomo, Muhajir YUSNITA YUSNITA